Physician-dentist and severe hemorrhage Jeanty, L
Bulletin de la Société des sciences médicales du Grand-Duché de Luxembourg
114, Številka:
2
Journal Article
Effects of Single Event Upsets (SEU) and Single Event Transients (SET) are studied in the FE-I4B chip of the innermost layer of the ATLAS pixel system. SEU/SET affect the FE-I4B Global Registers as ...well as the settings for the individual pixels, causing, among other things, occupancy losses, drops in the low voltage currents, noisy pixels, and silent pixels. Quantitative data analysis and simulations indicate that SET dominate over SEU on the load line of the memory. Operational issues and mitigation techniques are presented.
Highly Multiplexed Subcellular RNA Sequencing in Situ Lee, Je Hyuk; Daugharthy, Evan R.; Scheiman, Jonathan ...
Science (American Association for the Advancement of Science),
03/2014, Letnik:
343, Številka:
6177
Journal Article
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Understanding the spatial organization of gene expression with single-nucleotide resolution requires localizing the sequences of expressed RNA transcripts within a cell in situ. Here, we describe ...fluorescent in situ RNA sequencing (FISSEQ), in which stably cross-linked complementary DNA (cDNA) amplicons are sequenced within a biological sample. Using 30-base reads from 8102 genes in situ, we examined RNA expression and localization in human primary fibroblasts with a simulated wound-healing assay. FISSEQ is compatible with tissue sections and whole-mount embryos and reduces the limitations of optical resolution and noisy signals on single-molecule detection. Our platform enables massively parallel detection of genetic elements, including gene transcripts and molecular barcodes, and can be used to investigate cellular phenotype, gene regulation, and environment in situ.
For cognitive radio one of the most challenging issue is how to get the intelligence necessary to make the best possible allocation decision. Techniques such as sensing to get the information on the ...occupancy of the radio environment are being discussed. And designing terminals with scanning/sensing capabilities is a vibrant research topic; such terminals, and the sensing principles, can be very helpful when limited parts of spectrum are to be scanned. But in cases where there is no knowledge about the amount of spectrum to be scanned, this can result in time-and power-consuming operations. An alternative approach, described in this paper, consists of a "Cognition supporting Pilot Channel" (CPC) which directly provides relevant information to the terminal. This paper looks at the concept of this CPC, it initially describes the expected functionality and discusses the regulatory implications such a cognition supporting pilot channel, in the context of flexible spectrum management faces.
•Higher levels of social capital are associated with reductions in poverty.•The presence of poverty is a deterrent to building social capital.•Endogeneity and spatiality are critical dimensions of ...community well-being.•Impoverished areas should pair poverty reduction and social capital strategies.
This study explores the interrelationship between social capital and poverty, a negative indicator of well-being, in the Western United States. Econometric models that account for the endogeneity of poverty and social capital, spatial dependence, and cross-equation error correlation were used to explore two questions: is the presence of social capital associated with reduced poverty levels and does the presence of poverty impact social capital stocks? We found evidence that communities with higher social capital levels tend to have lower poverty rates and that poverty may pose barriers to social capital formation. This suggests that policies to reduce poverty will be more effective if coupled with policies to support social capital formation. The study's findings are particularly salient for communities in persistent poverty. These results emerged only after accounting for endogeneity and spatial relationships. Because many factors contributing to well-being are jointly determined with well-being and indicators of well-being are frequently spatially clustered, this situation is likely to be more common than has been typically recognized in the literature.
Glioblastoma (GBM) is the most aggressive primary brain tumor characterized by infiltrative growth of malignant glioma cells into the surrounding brain parenchyma. In this study, our analysis of GBM ...patient cohorts revealed a significantly higher expression of Glycosyltransferase 8 domain containing 1 (GLT8D1) compared to normal brain tissue and could be associated with impaired patient survival. Increased in vitro expression of GLT8D1 significantly enhanced migration of two different sphere-forming GBM cell lines. By in silico analysis we predicted the 3D-structure as well as the active site residues of GLT8D1. The introduction of point mutations in the predicted active site reduced its glycosyltransferase activity in vitro and consequently impaired GBM tumor cell migration. Examination of GLT8D1 interaction partners by LC-MS/MS implied proteins associated with cytoskeleton and intracellular transport as potential substrates. In conclusion, we demonstrated that the enzymatic activity of glycosyltransferase GLT8D1 promotes GBM cell migration.
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•The glycosyltransferase GLT8D1 is enriched in GBM tissue and cells•In silico analysis predicts the 3D structure and the active site of GLT8D1•Enzymatically active GLT8D1 promotes GBM migration•Manipulation of GLT8D1 enzymatic activity decreases GBM migration
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