We estimated the rate of reactivation tuberculosis (TB) in the United States, overall and by population subgroup, using data on TB cases and Mycobacterium tuberculosis isolate genotyping reported to ...the Centers for Disease Control and Prevention during 2006-2008. The rate of reactivation TB was defined as the number of non-genotypically clustered TB cases divided by the number of person-years at risk for reactivation due to prevalent latent TB infection (LTBI). LTBI was ascertained from tuberculin skin tests given during the 1999-2000 National Health and Nutrition Examination Survey. Clustering of TB cases was determined using TB genotyping data collected by the Centers for Disease Control and Prevention and analyzed via spatial scan statistic. Of the 39,920 TB cases reported during 2006-2008, 79.7% were attributed to reactivation. The overall rate of reactivation TB among persons with LTBI was estimated as 0.084 (95% confidence interval (CI): 0.083, 0.085) cases per 100 person-years. Rates among persons with and without human immunodeficiency virus coinfection were 1.82 (95% CI: 1.74, 1.89) and 0.073 (95% CI: 0.070, 0.075) cases per 100 person-years, respectively. The rate of reactivation TB among persons with LTBI was higher among foreign-born persons (0.098 cases/100 person-years; 95% CI: 0.096, 0.10) than among persons born in the United States (0.082 cases/100 person-years; 95% CI: 0.080, 0.083). Differences in rates of TB reactivation across subgroups support current recommendations for targeted testing and treatment of LTBI.
Women from racial and ethnic minority groups face a disproportionate burden of cervical and breast cancers in the United States. The Coronavirus Disease 2019 (COVID-19) pandemic might exacerbate ...these disparities as supply and demand for screening services are reduced. The National Breast and Cervical Cancer Early Detection Program (NBCCEDP) provides cancer screening services to women with low income and inadequate health insurance. We examined COVID-19's impact on NBCCEDP screening services during January-June 2020. We found the total number of NBCCEDP-funded breast and cervical cancer screening tests declined by 87% and 84%, respectively, during April 2020 compared with the previous 5-year averages for that month. The extent of declines varied by geography, race/ethnicity, and rurality. In April 2020, screening test volume declined most severely in Health and Human Services Region 2 - New York (96% for breast, 95% for cervical cancer screening) compared to the previous 5-year averages. The greatest declines were among American Indian/Alaskan Native women for breast cancer screening (98%) and Asian Pacific Islander women for cervical cancer screening (92%). Test volume began to recover in May and, by June 2020, NBCCEDP breast and cervical cancer screening test volume was 39% and 40% below the 5-year average for that month, respectively. However, breast cancer screening remained over 50% below the 5-year average among women in rural areas. NBCCEDP programs reported assisting health care providers resume screening.
•Women of color face a disproportionate burden of cervical and breast cancers in the United States.•The COVID-19 pandemic reduced breast and cervical cancer screening among low-income women.•Prolonged delays in screening due to COVID-19 threaten to increase disparities in cancer outcomes.
The cranial anatomy of the early non‐mammalian cynodont Galesaurus planiceps from the South African Karoo Basin is redescribed on the basis of a computed tomographic reconstruction of the skull. ...Previously, little was known about internal skull morphology and the nervous and sensory system of this taxon. The endocranial anatomy of various cynodonts has been intensively studied in recent years to understand the origin of mammalian characters in the nasal capsule, brain and ear. However, these studies have focused on only a few taxa, the earliest of which is another Early Triassic cynodont, Thrinaxodon liorhinus. Galesaurus is phylogenetically stemward of Thrinaxodon and thus provides a useful test of whether the mammal‐like features observed in Thrinaxodon were present even more basally in cynodont evolution. The cranial anatomy of G. planiceps is characterized by an intriguing mosaic of primitive and derived features within cynodonts. In contrast to the very similar internal nasal and braincase morphology of Galesaurus and Thrinaxodon, parts of the skull that seem to be fairly conservative in non‐prozostrodont cynodonts, the morphology of the maxillary canal differs markedly between these taxa. Unusually, the maxillary canal of Galesaurus has relatively few ramifications, more similar to those of probainognathian cynodonts than that of Thrinaxodon. However, its caudal section is very short, a primitive feature shared with gorgonopsians and therocephalians. The otic labyrinth of Galesaurus is generally similar to that of Thrinaxodon, but differs in some notable features (e.g. proportional size of the anterior semicircular canal). An extremely large, protruding paraflocculus of the brain and a distinct medioventrally located notch on the anterior surface of the tabular, which forms the dorsal border of the large parafloccular lobe, are unique to Galesaurus among therapsids with reconstructed endocasts. These features may represent autapomorphies of Galesaurus, but additional sampling is needed at the base of Cynodontia to test this.
The pollination services provided by bees are essential for supporting natural and agricultural ecosystems. However, bee population declines have been documented across the world. Many of the factors ...known to undermine bee health (e.g., poor nutrition) can decrease immunocompetence and, thereby, increase bees' susceptibility to diseases. Given the myriad of stressors that can exacerbate disease in wild bee populations, assessments of the relative impact of landscape habitat conditions on bee pathogen prevalence are needed to effectively conserve pollinator populations. Herein, we assess how landscape-level conditions, including various metrics of floral/nesting resources, insecticides, weather, and honey bee (Apis mellifera) abundance, drive variation in wild bumble bee (Bombus impatiens) pathogen loads. Specifically, we screened 890 bumble bee workers from varied habitats in Pennsylvania, USA for three pathogens (deformed wing virus, black queen cell virus, and Vairimorpha (= Nosema) bombi), Defensin expression, and body size. Bumble bees collected within low-quality landscapes exhibited the highest pathogen loads, with spring floral resources and nesting habitat availability serving as the main drivers. We also found higher loads of pathogens where honey bee apiaries are more abundant, a positive relationship between Vairimorpha loads and rainfall, and differences in pathogens by geographic region. Collectively, our results highlight the need to support high-quality landscapes (i.e., those with abundant floral/nesting resources) to maintain healthy wild bee populations.
L. is an important medicinal plant with documented use for the treatment of gout, headache and migraine reaching back to the Middle Ages. Triterpenoid saponins from roots and flowers are used in ...up-to-date phytotherapeutic treatment of bronchitis and colds due to their expectorant and secretolytic effects. In addition to the wild type plants with yellow petals, a red variant and an intermediate orange form of
L. have recently been found in a natural habitat. The secondary metabolite profiles of roots, leaves and flowers of these rare variants were investigated and compared with the wild type metabolome. Two flavonoids, six flavonoid glycosides, four novel methylated flavonoid glycosides, five anthocyanins and three triterpenoid saponins were identified in alcoholic extracts from the petals, leaves and roots of the three variants by high performance liquid chromatography (HPLC)-diode array detection (DAD)/mass spectrometry (MS
) analyses. Anthocyanins were detected in the petals of the red and orange variety, but not in the wild type. No other effects on the metabolite profiles of the three varieties have been observed. The possibility is discussed that a regulatory step of the anthocyanin biosynthetic pathway may have been affected by mutation thus triggering color polymorphism in the petals.
Essential oils (EOs) and their individual volatile organic constituents have been an inherent part of our civilization for thousands of years. They are widely used as fragrances in perfumes and ...cosmetics and contribute to a healthy diet, but also act as active ingredients of pharmaceutical products. Their antibacterial, antiviral, and anti-inflammatory properties have qualified EOs early on for both, the causal and symptomatic therapy of a number of diseases, but also for prevention. Obtained from natural, mostly plant materials, EOs constitute a typical example of a multicomponent mixture (more than one constituent substances, MOCS) with up to several hundreds of individual compounds, which in a sophisticated composition make up the property of a particular complete EO. The integrative use of EOs as MOCS will play a major role in human and veterinary medicine now and in the future and is already widely used in some cases,
e.g.
, in aromatherapy for the treatment of psychosomatic complaints, for inhalation in the treatment of respiratory diseases, or topically administered to manage adverse skin diseases. The diversity of molecules with different functionalities exhibits a broad range of multiple physical and chemical properties, which are the base of their multi-target activity as opposed to single isolated compounds. Whether and how such a broad-spectrum effect is reflected in natural mixtures and which kind of pharmacological potential they provide will be considered in the context of ONE Health in more detail in this review.
Tubulin tyrosine ligase (TTL) catalyzes the post-translational retyrosination of detyrosinated α-tubulin. Despite the indispensable role of TTL in cell and organism development, its molecular ...mechanism of action is poorly understood. By solving crystal structures of TTL in complex with tubulin, we here demonstrate that TTL binds to the α and β subunits of tubulin and recognizes the curved conformation of the dimer. Biochemical and cellular assays revealed that specific tubulin dimer recognition controls the activity of the enzyme, and as a consequence, neuronal development. The TTL-tubulin structure further illustrates how the enzyme binds the functionally crucial C-terminal tail sequence of α-tubulin and how this interaction catalyzes the tyrosination reaction. It also reveals how TTL discriminates between α- and β-tubulin, and between different post-translationally modified forms of α-tubulin. Together, our data suggest that TTL has specifically evolved to recognize and modify tubulin, thus highlighting a fundamental role of the evolutionary conserved tubulin tyrosination cycle in regulating the microtubule cytoskeleton.
•Interdiffusion coefficients within Mg(Al) and Mg(Zn) solid solutions.•Impurity diffusion of Al and Zn in Mg.•Interdiffusion coefficient in Mg(Zn) was higher than that of Mg(Al).•Zn impurity ...diffusion coefficient was higher than that of Al in Mg.
Interdiffusion and impurity diffusion in Mg binary solid solutions, Mg(Al) and Mg(Zn) were investigated at temperatures ranging from 623 to 723K. Interdiffusion coefficients were determined via the Boltzmann–Matano Method using solid-to-solid diffusion couples assembled with polycrystalline Mg and Mg(Al) or Mg(Zn) solid solutions. In addition, the Hall method was employed to extrapolate the impurity diffusion coefficients of Al and Zn in pure polycrystalline Mg. For all diffusion couples, electron microprobe analysis was utilized for the measurement of concentration profiles. The interdiffusion coefficient in Mg(Zn) was higher than that of Mg(Al) by an order of magnitude. Additionally, the interdiffusion coefficient increased significantly as a function of Al content in Mg(Al) solid solution, but very little with Zn content in Mg(Zn) solid solution. The activation energy and pre-exponential factor for the average effective interdiffusion coefficient in Mg(Al) solid solution were determined to be 186.8 (±0.9)kJ/mol and 7.69×10−1 (±1.80×10−1)m2/s, respectively, while those determined for Mg(Zn) solid solution were 139.5 (±4.0)kJ/mol and 1.48×10−3 (±1.13×10−3)m2/s. In Mg, the Zn impurity diffusion coefficient was an order of magnitude higher than the Al impurity diffusion coefficient. The activation energy and pre-exponential factor for diffusion of Al impurity in Mg were determined to be 139.3 (±14.8)kJ/mol and 6.25×10−5 (±5.37×10−4)m2/s, respectively, while those for diffusion of Zn impurity in Mg were determined to be 118.6 (±6.3)kJ/mol and 2.90×10−5 (±4.41×10−5)m2/s.
BACKGROUNDCulture-based diagnostics are the gold standard for diagnosing pulmonary TB (PTB). We characterized culture practices by comparing cases with documented sputum culture to those without. ...METHODSUsing multivariable logistic regression, we examined associations between PTB case characteristics and no documented sputum culture reported to the U.S. National TB Surveillance System during 2011-2021. RESULTSAmong 69,538 PTB cases analyzed, no sputum culture attempt was documented for 5,869 (8%). Non-sputum culture specimens were documented for 54%, 80%, and 89% of cases without documented sputum culture attempts among persons aged <15 years, 15-64, and 65+ years, respectively; bronchial fluid and lung tissue were common non-sputum specimens among cases in persons >15 years old. Having no documented sputum culture was associated with age <15 years (aOR 23.84, 99% CI 20.09-28.27) or ≥65 years (aOR 1.22, 99% CI 1.07-1.39), culture of a non-sputum specimen (aOR 6.57, 99% CI 5.93-7.28), residence in a long-term care facility (aOR 1.58, 99% CI 1.23-2.01), and receiving TB care outside of a health department (aOR 1.79, 99% CI 1.61-1.98). CONCLUSIONSInability to obtain sputum from children and higher diagnostic suspicion for disease processes that require tissue-based diagnostics could explain these findings. .
Abstract We present JWST MIRI Medium Resolution Spectrograph (MRS) observations of the β Pictoris system. We detect an infrared excess from the central unresolved point source from 5 to 7.5 μ m which ...is indicative of dust within the inner ∼7 au of the system. We perform point-spread function (PSF) subtraction on the MRS data cubes and detect a spatially resolved dust population emitting at 5 μ m. This spatially resolved hot dust population is best explained if the dust grains are in the small grain limit (2 π a ≪ λ ). The combination of unresolved and resolved dust at 5 μ m could suggest that dust grains are being produced in the inner few astronomical units of the system and are then radiatively driven outwards, where the particles could accrete onto the known planets in the system, β Pictoris b and c. We also report the detection of an emission line at 6.986 μ m that we attribute to Ar ii . We find that the Ar ii emission is spatially resolved with JWST and appears to be aligned with the dust disk. Through PSF-subtraction techniques, we detect β Pictoris b at the 5 σ level in our MRS data cubes and present the first mid-infrared spectrum of the planet from 5 to 7 μ m. The planet’s spectrum is consistent with having absorption from water vapor between 5 and 6.5 μ m. We perform atmosphere model grid fitting of the spectra and photometry of β Pictoris b and find that the planet’s atmosphere likely has a substellar C/O ratio.
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