Background Tumor cells in malignant pleural effusions (MPEs) are an important source of monocyte chemoattractant protein (MCP)-1. However, the role of tumor-derived MCP-1 in the pathogenesis and ...progression of MPE has not been determined. Methods B16 mouse skin melanoma cells, which are deficient in MCP-1 expression, and mouse Lewis lung cancer (LLC) cells, which express high levels of MCP-1, were engineered to stably express MCP-1 and short hairpin RNAs (shRNAs) targeting the MCP-1 transcript, respectively. Cells were injected into the pleural cavities of syngeneic immunocompetent mice, and MPE volume and pleural tumors were quantified at necropsy (day 14). MCP-1 and other mediators were determined by cytometric bead array and enzyme-linked immunosorbent assay, and mononuclear and endothelial cells were identified by immunolabeling of F4/80 and factor VIII–related antigen respectively. Mouse survival was assessed using Kaplan–Meier analysis. Vascular permeability in mice with MPE was assessed using albumin-binding Evans blue. Statistical tests were two-sided. Results LLC cells expressing shRNA against MCP-1 elaborated less than 5% of the MCP-1 level in cells expressing nonspecific shRNA (control cells), and intrapleural delivery of these cells resulted in less MPE (mean MPE volume = 86 and 585 μL, respectively; difference = 499 μL; 95% confidence interval CI = 331 to 669 μL; P < .001), reduced MCP-1 levels in the pleural fluid, and lower mortality than when control cells were delivered. Overexpression of MCP-1 in intrapleurally injected B16 melanoma cells led to increased MPE and reduced survival. In mice with MPE, MCP-1 was a potent inducer of vascular permeability, mononuclear recruitment, and, in pleural tumors, of angiogenesis. Conclusion MCP-1 produced by tumor cells is an important determinant of their capacity to induce the formation of MPE and may be a useful target for the treatment of malignant pleural disease.
Adsorptive wells arrays are an innovative outline of Permeable Reactive Barrier (PRBs) made of a definite number of passive deep wells opportunely distributed in the aquifer, known as PAB-D ...(Discontinuous Permeable Adsorptive Barrier). They are generally located downstream the contaminated groundwater flow and perpendicularly to the groundwater flow direction. Being PAB-D wells filled with adsorbing media, whose hydraulic permeability is higher than the surrounding media, the array will create a targeted capture zone, which will force the contaminated water to pass through the whole PAB-D, allowing for both the interception of the contaminant plume and its treatment. In this work, an optimized configuration of PAB-D is presented, for the in situ-remediation of an aquifer simultaneously contaminated by benzene and toluene. The design optimization of the PAB-D was performed by using COMSOL Multiphysics®, in which numerical simulations reproduced the transport and the adsorptive phenomena occurring inside the aquifer and the barrier itself. The proposed technique was applied to the remediation of an aquifer located in an urban area in the north of Naples (Italy), in proximity of numerous landfills, where the contamination was spread over an area of 0.10 km2. Simulation results confirm the effectiveness of the PAB-D, being both pollutant plumes intercepted and their concentrations reduced below their correspondent Italian regulatory threshold values. The best array configuration of PAB-D resulted made of 741 wells, each having a diameter of 0.6 m, which was also compared with a continuous barrier (PABC) showing a reduction of about 49% of the volume and 35% of the overall remediation cost.
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•BTEX polluted groundwater treatment via adsorptive Permeable Adsorptive Barriers (PABs).•Application of PABs in case of multiple contamination and in particular for benzene and toluene removal.•Numerical modelling of advective and reactive processes occurring in the barrier.•Optimization of the intervention with cost analysis.
Objective To examine transcutaneous bilirubin (TcB) levels in late preterm neonates. Study design Between July 2006 and December 2009, we performed 4387 TcB measurements with a BiliCheck ...bilirubinometer in 793 healthy late preterm neonates at designated times up to 120 postnatal hours. TcB percentiles are presented on an hour-specific nomogram. Mean increment TcB rates and the rates of increase for different percentiles are calculated as well. Results We present a percentile-based nomogram that reflects the natural history of TcB in late preterm neonates up to the fifth day of life. TcB levels demonstrated a different pattern of increase in neonates who developed significant hyperbilirubinemia compared with those who did not. However, the rates of TcB increase were quite similar up to age 48 hours, with a substantial overlap of TcB values between the two groups. Conclusions We developed of a TcB nomogram designated for hour-specific evaluation of hyperbilirubinemia in neonates born between 350/7 and 376/7 weeks' gestation.
Repaired tetralogy of Fallot (rTOF) has an excellent long-term prognosis; however, survival is somewhat less than normal. Of all the residual lesions and sequellae after rTOF, pulmonary regurgitation ...(PR) is the most important, correlating with right ventricular (RV) size, exercise intolerance and serious ventricular arrhythmias. Pulmonary valve replacement (PVR) has beneficial effects on RV size and function, provided it is performed early, before irreversible RV dysfunction ensues. Moreover, PVR is associated with an improvement in patients' symptoms and exercise tolerance and combined with arrhythmia surgery (cryoablation) it leads to a dramatic decrease in the incidence of fatal ventricular arrhythmias. Associated lesions, especially branch pulmonary artery stenosis, which aggravates PR, and tricuspid regurgitation, which further impacts on RV size and function, need addressing. Large right ventricular outflow (RVOT) akinetic and aneurysmal regions are frequent and further compromise RV function; therefore, resection during PVR should be attempted. Despite excellent mid-term results, homografts and xenografts, usually used for RVOT reconstruction, suffer late dysfunction and failure, committing patients and surgeons to further operations. Therefore, the decision to operate should be based on the balance between progressive RV dilatation, exercise intolerance, symptoms, arrhythmias and the fact that further reoperations will be needed. Research on the ideal valve for RVOT reconstruction is ongoing. Prospective follow-up of patients with rTOF with exercise testing and assessment of RV size and function, preferably with magnetic resonance, will define better the natural history of the disease and will probably provide firm guidelines for PVR timing especially in asymptomatic patients.
Mammographic density (MD) and malignant-appearing microcalcifications (MAMCs) represent the earliest mammographic findings of non-palpable breast carcinomas. Matrix proteoglycans versican and decorin ...are frequently over-expressed in various malignancies and are differently involved in the progression of cancer. In the present study, we have evaluated the expression of versican and decorin in non-palpable breast carcinomas and their association with high risk mammographic findings and tumor characteristics.
Three hundred and ten patients with non-palpable suspicious breast lesions, detected during screening mammography, were studied. Histological examination was carried out and the expression of decorin, versican, estrogen receptor α (ERα), progesterone receptor (PR) and c-erbB2 (HER-2/neu) was assessed by immunohistochemistry.
Histological examination showed 83 out of 310 (26.8%) carcinomas of various subtypes. Immunohistochemistry was carried out in 62/83 carcinomas. Decorin was accumulated in breast tissues with MD and MAMCs independently of the presence of malignancy. In contrast, versican was significantly increased only in carcinomas with MAMCs (median ± SE: 42.0 ± 9.1) and MD (22.5 ± 10.1) as compared to normal breast tissue with MAMCs (14.0 ± 5.8), MD (11.0 ± 4.4) and normal breast tissue without mammographic findings (10.0 ± 2.0). Elevated levels of versican were correlated with higher tumor grade and invasiveness in carcinomas with MD and MAMCs, whereas increased amounts of decorin were associated with in situ carcinomas in MAMCs. Stromal deposition of both proteoglycans was related to higher expression of ERα and PR in tumor cells only in MAMCs.
The specific accumulation of versican in breast tissue with high MD and MAMCs only in the presence of malignant transformation and its association with the aggressiveness of the tumor suggests its possible use as molecular marker in non-palpable breast carcinomas.
An activated carbon commercially available named HGR, produced by Calgon-Carbon Group, was used to adsorbe metallic mercury. The work is part of a wider research activity by the same group focused on ...the removal of metallic and divalent mercury from combustion flue gas. With respect to previously published papers, this one is aimed at studying in depth thermodynamic equilibria of metallic mercury adsorption onto a commercial activated carbon. The innovativeness lies in the wider operative conditions explored (temperature and mercury concentrations) and in the evaluation of kinetic and thermodynamic data for a commercially available adsorbing material. In detail, experimental runs were carried out on a laboratory-scale plant, in which Hg° vapors were supplied in a nitrogen gas stream at different temperature and mercury concentration. The gas phase was flowed through a fixed bed of adsorbent material. Adsorbate loading curves for different Hg° concentrations together with adsorption isotherms were achieved as a function of temperature (120, 150, 200°C) and Hg° concentrations (1.0−7.0 mg/m
3
). Experimental runs demonstrated satisfying results of the adsorption process, while Langmuir parameters were evaluated with gas-solid equilibrium data. Especially, they confirmed that adsorption capacity is a favored process in case of lower temperature and they showed that the adsorption heat was -20 kJ/mol. Furthermore, a numerical integration of differential equations that model the adsorption process was proposed. Scanning electron microscopy (SEM) investigation was an useful tool to investigate about fresh and saturated carbon areas. The comparison between them allowed identification of surface sites where mercury is adsorbed; these spots correspond to carbon areas where sulfur concentration is greater.
Implications: Mercury compounds can cause severe harm to human health and to the ecosystem. There are a lot of sources that emit mercury species to the atmosphere; the main ones are exhaust gases from coal combustion and municipal solid waste incineration. Furthermore, certain CO
2
capture processes, particularly oxyfuel combustion in a pulverized fuel coal-fired power station, produce a raw CO
2
product containing several contaminants, mainly water vapor, oxygen, and nitrogen but also mercury, that have to be almost completely removed; otherwise these would represent a strong drawback to the success of the process.
Wet limestone scrubbing is one of the most widely used flue gas desulfurization processes for control of sulfur dioxide emissions from combustion of fossil fuels. A key step in the process is forced ...oxidation in the scrubber loop, which substantially improves the dewatering properties of the sludge, leading to the formation of gypsum (CaSO
4·2H
2O). This paper deals with the experimental study of calcium bisulfite oxidation in the presence of catalysts (ferrous and cuprous ions) added to the reaction vessel. A laboratory-scale apparatus was used; the experiments were performed at a fixed oxygen partial pressure (21.3
kPa) and at a temperature of 45
°C. In particular, the effect of the simultaneous addition of both catalysts has been studied. The analysis of the experimental results, carried out by using the theory of mass transfer with a chemical reaction, indicates that the slow reaction regime has been explored, and the transition from the kinetic to the diffusional sub-regime has been reached. Experimental results have been compared with those obtained in the presence of the single catalytic species (Cu
+ alone and Fe
2+ alone), showing that copper is less effective than iron as a catalyst; no synergy between the two catalysts added simultaneously has been observed.
In this paper the technical feasibility of various adsorbents for mercury removal from contaminated waters has been studied. Adsorption isotherms of mercury ions in aqueous solution have been ...experimentally measured on a granular activated carbon (Aquacarb 207EA™), a char, a pozzolana and a yellow tuff. The experimental evidences show that the mercury capture capacity of yellow tuff and char is of few tenths of milligrams per gram of sorbent while for the pozzolana and the activated carbon this value is of the order of 1
mg/g of sorbent. Moreover, for a mercury concentration as high as 3000
μg/l the pozzolana shows the highest adsorption capacity. This result seems to be quite interesting, especially in consideration of the extremely low cost of this natural sorbent.
This paper presents an experimental study of calcium bisulfite oxidation, a key step in the wet limestone-gypsum flue gas desulfurization (FGD) process, in the presence of catalysts (e.g., cobalt ...ions and a mixture of ferrous and cobalt ions). A fundamental approach is followed, by reproducing a simplified synthetic FGD liquor in which both catalyst ions, alone or mixed together, are present. A laboratory-scale apparatus is used, in which sulfurous solution is contacted with a gas phase at a fixed oxygen partial pressure (21.3 kPa) and at different temperature levels (25, 45, and 55 °C). The experimental results are analyzed using the theory of gas-liquid mass transfer with chemical reaction, showing that the slow reaction regime is explored and the transition from the kinetic to the diffusional subregime is identified. The experimental results are compared with those obtained in the presence of other catalytic species (manganese and ferrous ions), showing that cobalt is effective in catalyzing the oxidation of calcium bisulfite to sulfate, but to a minor extent with respect to iron and manganese.
With the depletion of many commercial fish stocks and an increasing demand for marine protein for human consumption, cephalopods have become more important as a fishery resource. In EU waters, ...cephalopod stocks are not routinely assessed and exploitation of these species by large-scale fisheries is largely unregulated. For sustainable exploitation, adequate assessment and scientifically-supported management strategies are needed. However, there is still a lack of data on stock status and inadequate knowledge of the life history and ecology of these species. The present review examined more than 200 scientific articles, on life history and ecology of European cephalopods, published since 2013. It describes recent contributions to knowledge in the context of previously identified research priorities, along with recent advances towards sustainable fishing and aquaculture. It also identifies outstanding knowledge gaps. While some priority areas, such as the development of the species identification guides and evaluation of climate change impacts on cephalopods, have seen significant advances, other challenges remain for the future. These include monitoring of the life history traits and fishery status for the main commercially exploited species in the area, implementation of improved species identification methods during scientific surveys and fisheries monitoring, development of tools to identify stock units, and the study of the environmental and anthropogenic impacts on the stocks of cephalopods inhabiting European waters.
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