The adequacy of a nonlinear equivalent circuit for the electrical admittance of the skin was investigated in relation to the type of decomposition and the number of members in a power series ...approximating the nonlinear conductivity. This work showed that in the case of decompositions of nonlinear components in terms of voltage powers, the response function of the model was adequate for five or more decomposition components, while in the case of decompositions of nonlinear components in terms of test current powers, the response function of the model was inadequate over the whole range of the number of decomposition components studied. These results provide evidence supporting selection of a model for describing the electrical admittance of human skin.
The role of methylation of 9 miRNA genes in the pathogenesis of metastatic clear cell renal cell carcinoma was determined by quantitative methylation-specific PCR (MS-PCR). For 5 genes (
MIR125B-1
,
...MIR137
,
MIR193A
,
MIR34B/C
, and
MIR375
), a significant correlation of high methylation level with late (III-IV) stages, large size (T3+T4) of the tumor, and metastasis to lymph nodes and/or distant organs was revealed. For another group of genes (
MIR125B-1
,
MIR1258
,
MIR193A
,
MIR34B/C
, and
MIR375
), a statistically significant correlation of high methylation level with loss of differentiation in the tumor (G3-G4) was found, and the opposite pattern was found for
MIR203A
. A total of 7 microRNA genes (
MIR125B-1
,
MIR1258
,
MIR137
,
MIR193A
,
MIR203A
,
MIR34B/C
, and
MIR375
) were identified, the methylation of which is associated with the progression of metastatic clear cell renal cell carcinoma. For 6 of them (except
MIR34B/C
) these data were obtained for the first time. Thus, new factors of the development and progression of clear cell renal cell carcinoma were identified as potential biomarkers for the early diagnosis and prognosis of metastatic clear cell renal cell carcinoma.
Systemic analysis of the relationship between the levels of methylation of 21 microRNA genes and the parameters of breast cancer progression was performed on a representative sample of 91 paired ...specimens of breast cancer and histologically normal tissues and a system of markers for prediction of metastasis was proposed. A significant association of hypermethylation of 11 genes with late (III-IV) clinical stages was found, and for 6 genes (
MIR124-1
,
MIR127
,
MIR34B/C
,
MIR9-3
,
MIR1258
, and
MIR339
) this association was highly significant (
p
≤0.001, FDR=0.01). For
MIR9-3
and
MIR339
, an association with tumor size was demonstrated (
p
<0.001, FDR=0.01). No association of the levels of methylation of the analyzed microRNA genes with the degree of differentiation were found. An association with lymph node metastasis was established for 9 microRNA genes; the most significant association was shown for 6 genes
MIR125B-1
,
MIR127
,
MIR9-3
,
MIR339
,
MIR124-3
, and
MIR1258
(
p
<0.005, FDR=0.05). Based on these 6 genes, a marker system for predicting breast cancer metastasis was developed by ROC analysis. This system is characterized by 87% sensitivity and 77% specificity (AUC=0.894). The proposed system may have clinical application in the personalized treatment of breast cancer patients.
The search for interacting long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and mRNAs of protein-coding genes through the mechanism of competing endogenous RNAs in tumors of ovarian cancer ...patients was carried out. The levels of expression of 24 lncRNAs, 20 miRNAs, and 28 mRNAs of protein-coding genes involved in oncogenesis were determined by real-time PCR on a set of representative samples. Correlations between lncRNAs/miRNA and miRNA/mRNA levels in ovarian cancer samples were analyzed. We identified 8 pairs of lncRNAs/miRNA and 17 pairs of miRNA/mRNA, the expression levels of which have a negative correlation. Five triplets of potentially interacting lncRNAs/miRNA/mRNA have been identified, among which the most significant triplet is the
OIP5
-
AS1
/
miR-203a-3p
/
ZEB1
. The data obtained determine new epigenetic profiles, as well as new potential biomarkers and targets for targeted therapy of ovarian cancer patients.
Neutrons from DD fusion in the interelectrode space of a table‐top, low‐energy, nanosecond vacuum discharge with a deuterium‐loaded Pd anode have been demonstrated earlier. In addition, the principal ...role of a virtual cathode and the corresponding deep potential well formed in the interelectrode space are recognized under detailed particle‐in‐cell simulations of the discharge experimental conditions using a fully electrodynamic code. PIC modelling has allowed the identification of the scheme of small‐scale experiments with a rather old branch of plasma physics as the inertial electrostatic confinement fusion. The goal of this work is to present and discuss in detail the available experimental results on deuteron oscillations followed by pulsating DD neutron yield in this scheme based on nanosecond vacuum discharge. PIC simulations of some experimental regimes of pulsating neutron yield are also shown and discussed, as well as comparisons with an available similar scheme of periodical oscillating plasmas spheres for fusion.
The detection of genes related to the lifetime of patients with clear cell renal cancer provides information on the mechanisms of the tumor development and can be the basis for creating approaches to ...predict patient survival. In this paper, the expression of genes regulated by the HIF2α transcriptional factor was studied. Based on the results obtained here and previously identified genes regulated by the transcriptional factor HIF1α, a new panel of 6 genes, including the
BAP1
gene, was proposed. Expression of genes of this panel allows predicting the survival of patients with clear cell renal cancer with high sensitivity (93%), specificity (96%), and relative risk (21.5). After verification, the application of this panel can be useful for personalized treatment of patients with clear cell renal cancer, which will increase the effectiveness of therapy.
We have compared the impact of heavy metals (HMs: Cu 660 + Zn 1100 + Pb 650 mg/kg) on agrosoddy-podzolic soils (Albic Retisols (Loamic, Aric, Cutanic, Ochric)) of two arable fields (Chashnikovo, ...Moscow oblast) with different contents of organic carbon (C
org
3.86 and 1.30%) and different fertility levels using indicators of acute and chronic phytotoxicity. At the same level of polymetallic contamination, the responses of test plants to the presence of high concentrations of HMs and potential remediants (lignohumate and biochar) in soils of the same type with different C
org
contents noticeably differ with respect to plant growth parameters and metal accumulation in the phytomass. The HMs contamination of low-fertile soil leads to the complete death of plants in the pot experiment, while plants on highly fertile soil continued to develop until flowering with only slight deviations from the control. Experimental data on the contents of total and water-soluble forms of HMs and nutrients in the studied soils are presented. The relationships between the chemical composition of soils and the results of phytotests have been found using the principal component analysis. It is shown that the threefold difference in the content of C
org
between the soils of the same texture and acidity (pH) result in significantly different response of test plants to the same concentration of HMs. The necessity of correcting the standards for the permissible concentration of HMs in soils with due account for the C
org
content in addition to pH and texture is discussed.
An association was found between reduced expression of miR-34a, miR-146a with both metastasis to regional lymph nodes (relative risk RR=10.50 and RR=5.25, respectively) and the development of distant ...metastases (RR=9.50 and RR=4, 40, respectively) in gastric cancer. They are excellent classifiers: AUC>0.9 for both miRNAs. The association of miR-335 expression with metastasis to the lymph nodes is much weaker, but it is also a good classifier for identifying a group with distant metastasis (RR=5.90). A correlation was found between the expression of miR-34a and miR-146a during metastasis, which is absent in non-metastatic tumors. Thus, miR-34a, miR-146a, and miR-335 miRNAs can be proposed as candidates for biomarkers of the risk of gastric cancer metastasis.
—
In a laboratory experiment, the rate of mineralization of organic matter in samples of oil-contaminated and background soils formed in the main types of the middle-taiga biogeocenoses of Western ...Siberia was determined. The rate of mineralization was estimated by the amount of CO
2
released from wet samples at room temperature. The cumulative curves had a linear form. Therefore, the values of the average daily CO
2
release per 1 g of dry soil (
K
m
) were used to estimate the mineralization rate. The
K
m
values varied in the range of 0.1123–1.578 mg CO
2
/g per day. Minimum
K
m
values (<0.2) were typical of the oil-polluted forest soils with the maximum content of oil products; high
K
m
values (>0.8) were determined on the soils of uncontaminated background plots and oil-polluted plots of mesotrophic and eutrophic biogeocenoses with the neutral reaction and increased concentrations of biophilous elements. Moderate values (0.2 <
K
m
<0.8) were typical of the plots confined to initially oligotrophic bogs (ryams, ridged-hollow bog complexes) moderately polluted with oil. The
K
m
values negatively correlated with the contents of petroleum products (
K
= –0.50),
n
-alkanes (
K
= –0.73), and the coefficient of oil biodegradation (
K
= –0.72).
The problem of gene pool protection of wild plant species has gained a special relevance in modern times. In connection with the growth of population there is an increase in economic activity of ...human beings and their active influence on nature: wild lands plowing, overpasses plotting, construction of new and widening the boundaries of existing settlements. In addition, intensive harvesting of plants as medicinal, decorative, food, forage, etc is in progress. All this has a negative effect on the condition of wild plants: their stocks are declining, and some species are disappearing at all. A number of plants such as: spring adonis (
Adonis vernalis
L.), martagon lily
Liliumpilosisculum
(Freyn)) Miscz,L., butterfly orchid
Platantherabifolia
(L.) Rchb,
Paeoniaanomala
L. , rosewort (
Rhodiolarosea
L.) in their status, they belong to the group of rare and endangered species, they are listed in the Red Book of the Middle Urals, and their conservation requires introduction.
The study found that the species studied are culturally resistant, adapting well to new conditions. During the growth process, they went through all phenological phases, ending the development cycle with the fruiting phase with seed formation. Seeds with high enough sowing properties was provided by martagon lily, for which self-seeding is characteristic; low germination of seeds is noted in
Rhodiolarosea
L. and
Platantherabifolia
(L.) Rchb.
PDF