The objective of this study was to evaluate and compare the cytotoxicity and genotoxicity on human fibroblast cell lines of sodium hypochlorite (NaOCl), chitosan and propolis as root canal irrigating ...solutions. Human fibroblast cells were exposed to chitosan, propolis and NaOCl for 4 and 24 h. Cell viability was assessed by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide, and oxidative DNA damage was assessed by determination of 8-hydroxydeoxyguanosine (8-OHdG) level with an ELISA kit. The data of cell cytotoxicity were analysed statistically using a test of one-way analysis of variance at a significance level of p < 0.05. In the NaOCI group, the 8-OHdG level was higher than in the chitosan group, but there was no statistical difference when compared with the other groups (p < 0.05). It was determined that the irrigation solutions were cytotoxic, depending on the dose and time. NaOCl was the most toxic solution after both 4 and 24 h of exposure (p < 0.05). Chitosan and propolis may be alternatives to NaOCl for irrigation solutions, because they are both less toxic and produce less oxidative DNA damage.
It is very important that the dentists have sufficient information about possible variations in the expected root canal configurations in order to achieve success in endodontic treatment. In addition ...to having adequate knowledge on the variations of the root canal anatomy, periapical radiographs from different angles, careful examination of the pulp chamber floor, and use of dental operation microscope during the procedure are also important factors that contribute to the diagnosis of the additional roots and canals. The aims of this article are to present the diagnostic approach and root canal treatments of two maxillary first premolar teeth with three canals in two patients.
The aim of this study was to evaluate the efficacy of R-Endo® and K3® rotary nickel-titanium instruments compared with manual instrumentation with H-files, with use of a solvent, for removal of ...gutta-percha during retreatment.
Forty five freshly extracted human single-rooted teeth, each with one root canal, were instrumented with K-files and filled using cold lateral compaction of gutta-percha and AH 26® sealer. The teeth were randomly divided into three groups of 15 specimens each. Removal of gutta-percha was performed with the following devices and techniques: Group 1 (H-files), Group 2 (R-Endo®), and Group 3 (K3®). The specimens were rendered transparent for the evaluation of the area of remaining gutta-percha/sealer in buccolingual and mesiodistal directions. Statistical analysis as performed by using one-way ANOVA and Kruskal-Wallis tests (p=0.05).
All retreatment techniques used in this study left some filling material inside the root canal. Images in buccolingual and mesiodistal directions showed no significant differences between the groups (p>0.05).
Under the experimental conditions, the remaining filling material after retreatment was similar for each group.
The infected pulp, microorganisms toxins, metabolic products, chemical agents, mechanical irritations, foreign substances, trauma and host defense plays a role in the periapical tissue diseases. ...Options for the treatment of large periapical lesions range from non-surgical root canal treatment and/or apical surgery to extraction. Although apical surgery may be a treatment option in these cases, conventional root canal treatment should be the first choice. The aim of this case report is to report that the large size of a periapical lesion does not always need a surgical approach and even large periapical lesions heal following a conservative endodontic therapy.
Kırk adet yapay kanal rastgele iki gruba ayrıldı (n=20). Kanallar Mtwo ve S5 eğelerle apikal çap 30’a kadar şekillendirildi. Dijital kamera kullanılarak elde edilen preoperatif ve postoperatif kanal ...görüntüleri birleştirildi ve normalden sapmalar kaydedildi. Madde kaldırma 5 farklı noktada ölçüldü. İstatistiksel değerlendirme 0.05 anlamlılık düzeyinde Kolmogorov-Smirnov testi, independent t-testi ve ki-kare testi kullanılarak yapıldı.
BULGULAR: Kanalların ortalama toplam genişlikleri, şekillendirmenin apikal son noktası hariç Mtwo grubunda S5 grubundan anlamlı olarak daha fazlaydı (p
Amaç:
Bu çalışmada, QMix ™, etilendiamintetraasetik asit ve klorheksidin irrigasyon
solüsyonlarının human osteoblast hücreleri hattı üzerindeki zamana bağlı toksik
etkisi değerlendirdi.
Materyal ve ...metod: Human
osteoblast hücreleri, % 5 CO2 ve% 100 bağıl nem içeren bir ortamda 37ºC'de
tek tabakalı olacak şekilde kültüre edildi. Hücreler 4 saat ve 24 saat boyunca etilendiamintetraasetik
asit (EDTA), klorheksidin (CHX) ve QMix ™ 'e maruz bırakıldı. Hücre canlılığı
2,3-bis (2-metoksi-4-nitro-5-sülfenil) -5 - (fenilamino) karbonil -2H-tetrazolyum
hidroksit kiti (XTT) ile değerlendirildi. Human osteoblast hücrelerinin
ortalama yaşayabilirliğindeki farklılıklar istatistiksel olarak
değerlendirildi.
Bulgular:
Deney solüsyonlarındaki ve kontrol grubundaki canlı hücrelerin ortalama yüzdesi
arasında hem 4 saatlik (p˂0,001) hem de
24 saatlik (p =0,004) uygulamanın
sonrasında istatistiksel olarak anlamlı
fark görüldü. Canlı hücrelerin ortalama yüzdesi, EDTA, CHX ve QMixTM
grubunda uygulama süresinin artması ile istatistiksel olarak anlamlı derecede
azaldı (p˂0,05). 4 saat süresince uygulamanın ardından CHX ‘e göre EDTA ve Qmix
istatistiksel olarak anlamı derecede daha az toksik etki gösterdi
(p˂0,05). 24 saat süresince uygulamanın
ardından ise irrigasyon solüsyonlarının toksisitesi arasında istatistiksel
olarak anlamlı fark bulunmadı (p>0,05).
Sonuç: Bu
çalışmada kullanılan irrigasyon solüsyonları human osteoblast hücre hattı
üzerinde çeşitli derecede toksik etki gösterdi. Uygulama süresinin artması kullanılan
irrigasyon solüsyonlarının human
osteoblast hücre hattı üzerindeki toksisitesini de arttırdı.
Amaç:
Bu çalışmada, QMix ™, etilendiamintetraasetik asit ve klorheksidin irrigasyon
solüsyonlarının human osteoblast hücreleri hattı üzerindeki zamana bağlı toksik
etkisi değerlendirdi.Materyal ve metod: Human
osteoblast hücreleri, % 5 CO2 ve% 100 bağıl nem içeren bir ortamda 37ºC'de
tek tabakalı olacak şekilde kültüre edildi. Hücreler 4 saat ve 24 saat boyunca etilendiamintetraasetik
asit (EDTA), klorheksidin (CHX) ve QMix ™ 'e maruz bırakıldı. Hücre canlılığı
2,3-bis (2-metoksi-4-nitro-5-sülfenil) -5 - (fenilamino) karbonil -2H-tetrazolyum
hidroksit kiti (XTT) ile değerlendirildi. Human osteoblast hücrelerinin
ortalama yaşayabilirliğindeki farklılıklar istatistiksel olarak
değerlendirildi.Bulgular:
Deney solüsyonlarındaki ve kontrol grubundaki canlı hücrelerin ortalama yüzdesi
arasında hem 4 saatlik (p˂0,001) hem de
24 saatlik (p =0,004) uygulamanın
sonrasında istatistiksel olarak anlamlı
fark görüldü. Canlı hücrelerin ortalama yüzdesi, EDTA, CHX ve QMixTM
grubunda uygulama süresinin artması ile istatistiksel olarak anlamlı derecede
azaldı (p˂0,05). 4 saat süresince uygulamanın ardından CHX ‘e göre EDTA ve Qmix
istatistiksel olarak anlamı derecede daha az toksik etki gösterdi
(p˂0,05). 24 saat süresince uygulamanın
ardından ise irrigasyon solüsyonlarının toksisitesi arasında istatistiksel
olarak anlamlı fark bulunmadı (p>0,05).
Sonuç: Bu
çalışmada kullanılan irrigasyon solüsyonları human osteoblast hücre hattı
üzerinde çeşitli derecede toksik etki gösterdi. Uygulama süresinin artması kullanılan
irrigasyon solüsyonlarının human osteoblast hücre hattı üzerindeki toksisitesini
de arttırdı.
Abstract Background/purpose The aim of the this study was to compare the amount of debris apically extruded during endodontic retreatment using two rotary nickel–titanium (NiTi) instruments (K3 and ...R-Endo) and Hedström files. Materials and methods Forty-five extracted human mandibular premolar teeth were used in this study. Root canals of the teeth were filled before being randomly assigned to three groups. In group 1, gutta-percha was removed with K3 (SybronEndo, West Collins, CA, USA) rotary NiTi instruments. In group 2, gutta-percha was removed with R-Endo (Micro-Mega, Besançon, France) rotary NiTi instruments. In group 3, gutta-percha was removed using Hedström files (Dentsply Maillefer, Ballaigues, Switzerland). Debris extruded from the apical foramen was collected into Eppendorf tubes. The liquid inside the tubes was dried, and the debris in each group was weighed and compared. Data were statistically analyzed using one-way analysis of variance, and Kruskal–Wallis test and Mann–Whitney U test. Results Although all retreatment techniques resulted in apical extrusion, groups 1 and 2 produced significantly less apical extrusion than group 3 (P < 0.05). No statistically significant difference was found between groups 1 and 2 (P > 0.05). Conclusion All retreatment techniques produced extruded debris during endodontic retreatment; however, both rotary NiTi systems were associated with less apical extrusion than manual instrumentation with Hedström files.
Objectives: The aim was to determine the strength of signals derived from the dental pulp and those from the surrounding tissues in human teeth using the laser Doppler flowmetry (LDF).
Methods: ...Twenty-six vital teeth (13 experimental and 13 controls) from 12 patients were used. Experimental teeth had root canal treatment in a single visit and their healthy antimeres were controls. Blood flow measurements were made before and after local anaesthesia, after root canal preparation, following root canal filling and coronal restoration.
Results: Perfusion unit (PU) values recorded from empty pulp chamber were determined to be only 30% lower than the values recorded before anaesthesia from the experimental intact teeth. When the measurements in the group were compared with each other the values of variation in the both groups were not statistically significant. The variation between the groups when the measurement values before anaesthesia, 10
min after anaesthesia and after coronal restoration were compared, it was not statistically significant (
P>0.05). The variation between the groups after preparation and root canal filling in their values of measurements was important statistically (
P<0.05). The measurement values of the study group were lower than that of the control group.
Conclusions: In pulpal, LDF a major portion of the signal comes from tissues other than pulp. Without taking precautions (such as rubber-dam application) in pulp blood flow measurements, the results may be inconsistent.
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