The Rights of Nature (RoN) promote a new understanding of the human environment, where natural entities are conceived as subjects with intrinsic value independent of human interests. The ...implementation of this idea gained momentum in the United States in 2006. One decade and a half later, the idea has spread all over the world. Despite some efforts, a sophisticated geographical inventory of the movement is missing. Building on Kauffman (2020), we identified and analysed 409 initiatives in 39 countries, creating the most comprehensive database of RoN initiatives to date. We developed a taxonomy that may guide further research. We also present two detailed maps which can help policymakers, legislators, judges, researchers, and the public at large to evaluate and compare initiatives. The findings of this investigation directly help the UN Harmony with Nature Programme and have contributed to the launch of the Eco-Jurisprudence Monitor, an online database of RoN initiatives.
The purpose of this study was to design and synthesize Palladium nanoparticles (PdNPs) using an environmentally friendly approach and evaluate the in vitro efficacy of PdNPs in human ovarian cancer ...A2780 cells. Ultraviolet-Visible (UV-Vis) spectroscopy was used to monitor the conversion of Pd(II) ions to Pd(0)NPs. X-ray diffraction (XRD) revealed the crystallinity of the as-synthesized PdNPs and Fourier transform infrared spectroscopy (FTIR) further confirmed the role of the leaf extract of Evolvulus alsinoides as a reducing and stabilizing agent for the synthesis of PdNPs. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) showed that the average size of the NPs was 5 nm. After a 24-h exposure to PdNPs, cell viability and light microscopy assays revealed the dose-dependent toxicity of the PdNPs. Furthermore, the dose-dependent cytotoxicity of the PdNPs was confirmed by lactate dehydrogenase (LDH), increased reactive oxygen species (ROS) generation, activation of PdNPs-induced autophagy, impairment of mitochondrial membrane potential (MMP), enhanced caspase-3 activity, and detection of TUNEL-positive cells. Our study demonstrates a single, simple, dependable and green approach for the synthesis of PdNPs using leaf extracts of Evolvulus alsinoides. Furthermore, the in vitro efficacy of PdNPs in human ovarian cancer cells suggests that it could be an effective therapeutic agent for cancer therapy.
Sustainable cultivation and safe agricultural production are needed to ensure the safety of all living organisms and ecosystems. Most agricultural foods currently contain unacceptable levels of ...pesticide residues. In an attempt to ensure pest control and high crop yields, farmers spray a large number of pesticides in quantities that exceed the safety limits for agricultural crops. Pesticide residues are highly toxic to humans, causing severe and even deadly diseases. Traditional analytical strategies for laboratory pesticide detection are often limited because they are time‐consuming and require trained personnel, which makes them very costly. Hence, accurate, rapid, and on‐site analysis of pesticides is drawing increasing attention for food safety reasons. Therefore, on‐site or point‐of‐care (POC) detection of pesticide residues has become an approach of paramount importance. Recently, various portable detection technologies, such as colorimetric, fluorescence, electrochemical, surface plasmon resonance, chemiluminescence, phosphorescence, microfluidic, and surface‐enhanced Raman scattering techniques, have been designed for on‐site monitoring of pesticide residues in vegetables and fruits. These devices have demonstrated great power of detection in preclinical agricultural settings. This review highlights the emerging insights and novel advances in portable devices, POC technologies, and on‐site sensing approaches for the detection of pesticide residues in agricultural foods. However, greater rigor in the design of portable pesticide‐detection devices is essential. The increasing demand for rapid detection of hazardous pesticide components has prompted a timely opportunity to summarize recent developments and progress in portable devices and on‐site sensing strategies to evaluate food safety.
Highlighted the emerging insights and novel advances in portable devices, point‐of‐care technologies, and on‐site sensing strategies for the detection of pesticide residues in agricultural foods. The developed sensing strategies have demonstrated great power of detection in preclinical agricultural settings.
Graphene oxide (GO) is a monolayer of carbon atoms that form a dense honeycomb structure, consisting of hydroxyl and epoxide functional groups on the two accessible sides and carboxylic groups at the ...edges. In contrast, graphene is a two-dimensional sheet of sp2-hybridized carbon atoms packed into a honeycomb lattice. Graphene has great potential for use in biomedical applications due to its excellent physical and chemical properties. In this study, we report a facile and environmentally friendly approach for the synthesis of reduced graphene oxide (rGO) using uric acid (UA). The synthesized uric acid-reduced graphene oxide (UA-rGO) was fully characterized by ultraviolet-visible (UV-Vis) absorption spectra, X-ray diffraction (XRD), dynamic light scattering (DLS), Fourier transform infrared (FTIR), scanning electron microscopy (SEM), and Raman spectroscopy. GO and UA-rGO induced a dose-dependent decrease in cell viability and induced cytotoxicity in human ovarian cancer cells. The results from this study suggest that UA-rGO could cause apoptosis in mammalian cells. The toxicity of UA-rGO is significantly higher than GO. Based on our findings, UA-rGO shows cytotoxic effects against human ovarian cancer cells, and its synthesis is environmentally friendly. UA-rGO significantly inhibits cell viability by increasing lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) generation, activation of caspase-3, and DNA fragmentation. This is the first report to describe the comprehensive effects of UA-rGO in ovarian cancer cells. We believe that the functional aspects of newly synthesized UA-rGO will provide advances towards various biomedical applications in the near future.
Ginsenosides are active components found abundantly in ginseng which has been used as a medicinal herb to modify disease status for thousands of years. However, the pharmacological activity of ...ginsenoside Re in the neuronal system remains to be elucidated. Neuroprotective activity of ginsenoside Re was investigated in SH-SY5Y cells exposed to 6-hydroxydopamine (6-OHDA) to induce cellular injury. Ginsenoside Re significantly inhibited 6-OHDA-triggered cellular damage as judged by analysis of tetrazolium dye reduction and lactose dehydrogenase release. In addition, ginsenoside Re induced the expression of the antioxidant protein glutathione peroxidase 4 (GPX4) but not catalase, glutathione peroxidase 1, glutathione reductase, or superoxide dismutase-1. Furthermore, upregulation of GPX4 by ginsenoside Re was mediated by phosphoinositide 3-kinase and extracellular signal-regulated kinase but not by p38 mitogen-activated protein kinase or c-Jun N-terminal kinase. Ginsenoside Re also suppressed 6-OHDA-triggered cellular accumulation of reactive oxygen species and peroxidation of membrane lipids. The GPX4 inhibitor (1S,3R)-RSL3 reversed ginsenoside Re-mediated inhibition of cellular damage in SH-SY5Y cells exposed to 6-OHDA, indicating that the neuronal activity of ginsenoside Re is due to upregulation of GPX4. These findings suggest that ginsenoside Re-dependent upregulation of GPX4 reduces oxidative stress and thereby alleviates 6-OHDA-induced neuronal damage.
Basella alba, a green leafy vegetable with remarkable nutraceutical potential is widely used since ancient times to maintain a healthy colon. This plant has been investigated for its medicinal ...potential due to the increase in young adult cases of colorectal cancer each year. This study was accomplished to investigateBasella albamethanolic extract (BaME) antioxidantand anticancer properties. BaME consisted of a substantial amount of both phenolic and flavonoid compounds which exhibited significant antioxidant reactivity. Both colon cancer cell lines experienced a cell cycle arrest at the G0/G1 phase after receiving treatment with BaME, which inhibited pRb and cyclin D1 and raised p21 expression levels. This was associated with the survival pathway molecule inhibition and downregulation of E2F-1. The results of the current investigation confirm that BaME inhibits CRC cell survival and expansion. To conclude, thebioactive principles in the extract act as potential antioxidants and antiproliferative agents against colorectal cancer.
Silver nanoparticles (AgNPs) possess unique physical, chemical, and biological properties. AgNPs have been increasingly used as anticancer, antiangiogenic, and antibacterial agents for the treatment ...of bacterial infections in open wounds as well as in ointments, bandages, and wound dressings. The present study aimed to investigate the effects of two different sizes of AgNPs (10 nm and 20 nm) in male somatic Leydig (TM3) and Sertoli (TM4) cells and spermatogonial stem cells (SSCs).
Here, we demonstrate a green and simple method for the synthesis of AgNPs using Bacillus cereus culture supernatants. The synthesized AgNPs were characterized using ultraviolet and visible absorption spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and transmission electron microscopy (TEM). The toxicity of the synthesized AgNPs was evaluated by the effects on cell viability, metabolic activity, oxidative stress, apoptosis, and expression of genes encoding steroidogenic and tight junction proteins.
AgNPs inhibited the viability and proliferation of TM3 and TM4 cells in a dose- and size-dependent manner by damaging cell membranes and inducing the generation of reactive oxygen species, which in turn affected SSC growth on TM3 and TM4 as feeder cells. Small AgNPs (10 nm) were more cytotoxic than medium-sized nanoparticles (20 nm). TEM revealed the presence of AgNPs in the cell cytoplasm and nucleus, and detected mitochondrial damage and enhanced formation of autosomes and autolysosomes in the AgNP-treated cells. Flow cytometry analysis using Annexin V/propidium iodide staining showed massive cell death by apoptosis or necrosis. Real-time polymerase chain reaction and western blot analyses indicated that in TM3 and TM4 cells, AgNPs activated the p53, p38, and pErk1/2 signaling pathways and significantly downregulated the expression of genes related to testosterone synthesis (TM3) and tight junctions (TM4). Furthermore, the exposure of TM3 and TM4 cells to AgNPs inhibited proliferation and self-renewal of SSCs.
Our results suggest that AgNPs exhibit size-dependent nanoreprotoxicity in male somatic cells and SSCs, strongly suggesting that applications of AgNPs in commercial products must be carefully evaluated. Further studies of AgNPs-induced nanoreprotoxicity in animal models are required.
Ferroptosis is a recently recognized process of cell death characterized by accumulation of iron-dependent lipid peroxides. Herein, we demonstrate that peroxisome proliferator-activated receptor δ ...(PPARδ) inhibits ferroptosis of mouse embryonic fibroblasts (MEFs) derived from cysteine/glutamate transporter (xCT)-knockout mice. Activation of PPARδ by the specific ligand GW501516 led to a dose-dependent decrease in ferroptotic cell death triggered by xCT deficiency, along with decreased levels of intracellular iron accumulation and lipid peroxidation. These effects of GW501516 were abolished by PPARδ-targeting small interfering RNA (siRNA) and the PPARδ inhibitor GSK0660, indicating that PPARδ inhibits xCT deficiency-induced ferroptosis. In addition, GW501516-activated PPARδ time- and dose-dependently upregulated catalase expression at both the mRNA and protein levels. This PPARδ-mediated upregulation of catalase was markedly attenuated in cells treated with PPARδ-targeting siRNA and GSK0660, indicating that expression of catalase is dependent on PPARδ. Consistently, the effects of GW501516 on ferroptosis of xCT-deficient MEFs were counteracted in the presence of 3-amino-1,2,4-triazole, a specific inhibitor of catalase, suggesting that catalase is essential for the effect of PPARδ on ferroptosis triggered by xCT deficiency. GW501516-activated PPARδ stabilized peroxisomes through catalase upregulation by targeting peroxisomal hydrogen peroxide-mediated lysosomal rupture, which led to ferroptosis of xCT-deficient MEFs. Collectively, these results demonstrate that PPARδ modulates ferroptotic signals in xCT-deficient MEFs by regulating catalase expression.
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•PPARδ rescues MEFs from xCT deficiency which triggers ferroptotic cell death.•PPARδ upregulates the expression of catalase, an oxidoreductase typically expressed in peroxisomes.•PPARδ stabilizes the peroxisomes through cellular redox homeostasis.•PPARδ prevents lysosomal rupture by quenching peroxisomal hydrogen peroxide.
Scheme 1. Antibacterial and cytotoxic effect of NS-IONPs.
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•Green synthesis of uniformly-sized (5–6 nm) iron oxide nanoparticles (NS-IONP)•Polyphenols of Nigella sativa seeds act as ...reducing agents to synthesize NS-IONP.•NS-IONP promote growth inhibition in gram negative bacteria, lung cancer cells.•Prominent BAX expression and cell necrosis upon NS-IONPs accumulation in cells.•In vitro analyses proved NS-IONPs excellent antibacterial and anticancer activity.•Enhanced therapeutic efficacy due to efficient cellular uptake of NS-IONPs.
Several metal-based nanoparticles (NPs) have been found to be toxic and are known to exert adverse health outcomes with irreversible side effects. This highlights the need to discover effective,stable, and biocompatibletherapeutic components using natural sources.Here, a hexane extract ofNigella sativa seeds was used to synthesize iron oxide NPs (NS-IONPs) embedded with N. sativa phytoconstituents.The extract acted as a reducing agent that restricted the size of the NS-IONPs to 5–6 nm, signifying the potential to be cleared through the renal system.The fabricated NS-IONPs had a prominent effect on pathogenic gram-negative bacteria, E. coli (19.3 mm) and Salmonella typhi (14.2 mm) and lung cancer cells (lowest IC50of 18.75 µg/mL) mainly by binding to the phospholipid components of the cell membrane. This resulted in cell shrinkage and further inhibited cell growth. Transmission electron microscopy analyses revealed that themechanisms of cellular NP uptake varied depending on the celltype. Accumulation of NS-IONPs inside the cell increased BAX expression and arrested the cells at the G0/G1 phase, thereby conspicuously extending the G0 phase to initiate necrosis. Thus, these finding suggest that the synthesized NS-IONPs exhibited high antibacterial activity and effective cytotoxicity against cancer cell lines A549 and HCT116 compared to IONPs. The innovation of the current study is that the biogenic fabrication of IONPs is simple and cost effective results in stable nanomaterial, NS-IONPs with potential antibacterial and anticancer activity, which can be explored furthermore for various biomedical applications.
Insight about the impact of malathion on human physiology is still a challenge in environmental health. The present work was focused on the neurotoxic effects of malathion followed by impact of Aloe ...vera, if any, which is not known. The malathion has significantly altered the levels of the body weight, brain weight and relative weight of the brain. The significant levels of alteration were also observed in the levels of antioxidant potential and oxidative stress biomarkers such as NO, PCO, MDA, GSH, SOD, CAT, GST, and GPx antioxidants. The increased levels of SOD, CAT, GPx and decreased levels of GST were observed in the malathion treated experimental rats. In addition of these, the contents of inflammatory markers such as IL-6, COX-2, TNF-α, and NF-κB were also found to be altered significantly. A significant alteration was also recorded in the activity of AChE. The histological examination of liver tissue sections revealed the severe injury to the central vein and hepatic cords due to malathion toxicity. However, the pre-administration of A. vera markedly ameliorated the neurotoxic effect of malathion. These results suggested that the metabolites present in A. vera may be utilized as a potential and sustainable supplement in the proper management of pesticide induced neurotoxicity in association with the relevant therapeutics.
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