We discuss the main properties of the Galactic globular cluster (GC)blue straggler stars (BSSs), as inferred from our new catalog containingnearly 3000 BSSs. The catalog has been extracted from ...thephotometrically homogeneous V versus (B-V) color-magnitude diagrams(CMDs) of 56 GCs, based on Wide Field Planetary Camera 2 images of theircentral cores. In our analysis, we used consistent relative distancesbased on the same photometry and calibration. The number of BSSs hasbeen normalized to obtain relative frequencies (F BSS ) andspecific densities (N S ) using different stellar populationsextracted from the CMD. The cluster F BSS is significantlysmaller than the relative frequency of field BSSs. We find a significantanticorrelation between the BSS relative frequency in a cluster and itstotal absolute luminosity (mass). There is no statistically significanttrend between the BSS frequency and the expected collision rate. Thevalue of F BSS does not depend on other cluster parameters,apart from a mild dependence on the central density. Post-core-collapseclusters act like normal clusters as far as the BSS frequency isconcerned. We also show that the BSS luminosity function for the mostluminous clusters is significantly different, with a brighter peak andextending to brighter luminosities than in the less luminous clusters.These results imply that the efficiency of BSS production mechanisms andtheir relative importance vary with the cluster mass.Based on observations with the NASA/ESA Hubble Space Telescope, obtainedat the Space Telescope Science Institute, which is operated by theAssociation of Universities for Research in Astronomy, Inc., under NASAcontract NAS 5-26555.
In Hubble Space Telescope (HST) Cycle 17, we imaged the well-known globular star cluster 47 Tucanae for 121 orbits using the Wide Field Channel of the Advanced Camera for Surveys (ACS) and both the ...UV/visible (UVIS) and IR channels of the newly installed Wide Field Camera 3 (WFC3) instrument (GO-11677, PI: H. Richer). This unique data set was obtained to address many scientific questions that demand a very deep, panchromatic, and panoramic view of the cluster's stellar populations. In total, the program obtained over 0.75 Ms of imaging exposure time with the three HST cameras, over a time span of 9 months in 2010. The primary ACS field was imaged in the two broadband filters F606W and F814W, at 13 orientations, for all 121 orbits. The parallel WFC3 imaging provides a panchromatic (0.4-1.7 Delta *mm) and contiguous imaging swath over a 250? azimuthal range at impact radii of 6.5-17.9 pc in 47 Tuc. This imaging totals over 60 arcmin2 in area and utilizes the F390W and F606W broadband filters on WFC3/UVIS and the F110W and F160W broadband filters on WFC3/IR. In this paper, we describe the observational design of the new survey and one of the methods used to analyze all of the imaging data. This analysis combines over 700 full-frame images taken with the three HST cameras into a handful of ultra-deep, well-sampled combined images in each of the six filters. We discuss in detail the methods used to calculate accurate transformations that provide optimal alignment of the input images, the methods used to perform sky background offsets in the input stack and the flagging of deviant pixels, and the balance reached between the input-pixel drop size onto an output supersampled pixel grid. Careful photometric, morphological, and astrometric measurements are performed on the stacks using iterative PSF-fitting techniques, and reveal unprecedented color-magnitude diagrams of the cluster extending to >30th magnitude in the optical, 29th magnitude in the UV, and 27th magnitude in the IR. The data set provides a characterization of the complete stellar populations of 47 Tuc, extending from the faintest hydrogen-burning dwarfs through the main-sequence and giant branches down to very cool white dwarf remnants in the cluster. The imaging also provides the deepest probe of the stellar populations of the background Small Magellanic Cloud galaxy, resolving low-mass main-sequence dwarfs with M 0.2 M .
We engineered the oncolytic Salmonella typhimurium-derived bacterium VNP20009 as a vector to target delivery to tumors of the prodrug-activating enzyme carboxypeptidase G2 (CPG2) and to show enhanced ...antitumor efficacy on administration of different prodrugs.
We characterized CPG2 expression in vectors by immunoblotting, immunofluorescence, and enzyme activity. We assessed prodrug activation by high-performance liquid chromatography. Target human tumor cell and bacterial vector cell cytotoxicity was measured by flow cytometry and colony-forming assays. Therapy was shown in two human tumor xenografts and one mouse allograft with postmortem analysis of bacterial and CPG2 concentration in the tumors.
CPG2 is expressed within the bacterial periplasm. It activates prodrugs and induces cytotoxicity in human tumor cells but not in host bacteria. Following systemic administration, bacteria multiply within xenografts reaching 2 x 10(7)/g to 2 x 10(8)/g at 40 days postinoculation. The concentration of CPG2 in these tumors increases steadily to therapeutic levels of 1 to 6 units/g. The bacteria alone reduce the growth of the tumors. Subsequent administration of prodrugs further reduces significantly the growth of the xenografts.
The bacteria multiply within tumors, resulting in a selective expression of CPG2. The CPG2-expressing bacteria alone reduce the growth of tumors. However, in the presence of prodrugs activated by CPG2, this oncolytic effect is greatly increased. We conclude that bacterial oncolytic therapy, combined with CPG2-mediated prodrug activation, has great potential in the treatment of a range of cancers.
Genetically modified bacteria are a potentially powerful anticancer therapy due to their tumor targeting capacity, inherent antitumor activity, and ability to serve as efficient vectors for gene ...delivery. This study sought to characterize the acute and short-term toxicities and tumor colonization rates of a genetically modified Salmonella typhimurium (VNP20009) in dogs with spontaneous tumors, in the context of a phase I dose escalation trial.
Forty-one pet dogs with a variety of malignant tumors received weekly or biweekly i.v. infusions of VNP20009, at doses ranging from 1.5 x 10(5) to 1 x 10(8) cfu/kg. Vital signs and clinicopathologic variables were monitored regularly. Incisional biopsies were obtained before and 1 week following the first infusion for histopathology and bacterial culture.
The nominal maximum tolerated dose was 3 x 10(7) cfu/kg, with refractory fever and vomiting being the dose-limiting toxicities. One treatment-related acute death occurred. Bacteria were cultured from tumor tissue in 42% of cases. Thirty-five patients were evaluable for antitumor response. Major antitumor responses were seen in 15% (4 complete response and 2 partial response), and disease stabilization for at least 6 weeks in 10%.
Administration of VNP20009 at doses with acceptable toxicity results in detectable bacterial colonization of tumor tissue and significant antitumor activity in tumor-bearing dogs.
The White Dwarf Cooling Sequence in NGC 6791 Bedin, Luigi R; Salaris, Maurizio; Piotto, Giampaolo ...
The Astrophysical journal,
05/2005, Letnik:
624, Številka:
1
Journal Article
Using Hubble Space Telescope Advanced Camera for Surveys Wide Field Channel images, we derive the color-magnitude diagram of the old, metal-rich open cluster NGC 6791 to nearly 29th magnitude in V, ...which is the neighborhood of the hydrogen-burning limit. Comparison with isochrones leads to a discussion of the distance modulus, the reddening, and the age of the cluster. By making a statistical correction for field stars, we derive a preliminary luminosity function and a very tentative mass function. The white dwarf sequence is clearly shown and has been discussed in a separate paper.
Probing the Faintest Stars in a Globular Star Cluster Richer, Harvey B; Anderson, Jay; Brewer, James ...
Science (American Association for the Advancement of Science),
08/2006, Letnik:
313, Številka:
5789
Journal Article
Recenzirano
Odprti dostop
NGC 6397 is the second closest globular star cluster to the Sun. Using 5 days of time on the Hubble Space Telescope, we have constructed an ultradeep color-magnitude diagram for this cluster. We see ...a clear truncation in each of its two major stellar sequences. Faint red main-sequence stars run out well above our observational limit and near to the theoretical prediction for the lowest mass stars capable of stable hydrogen burning in their cores. We also see a truncation in the number counts of faint blue stars, namely white dwarfs. This reflects the limit to which the bulk of the white dwarfs can cool over the lifetime of the cluster. There is also a turn toward bluer colors in the least luminous of these objects. This was predicted for the very coolest white dwarfs with hydrogen-rich atmospheres as the formation of H₂ and the resultant collision-induced absorption cause their atmospheres to become largely opaque to infrared radiation.
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