The effects of consumption of n-3 polyunsaturated fatty acids (n-3 PUFAs) enriched hen eggs on endothelium-dependent and endothelium-independent vasodilation in microcirculation, and on endothelial ...activation and inflammation were determined in young healthy individuals. Control group (
= 21) ate three regular hen eggs/daily (249 mg n-3 PUFAs/day), and n-3 PUFAs group (
= 19) ate three n-3 PUFAs enriched hen eggs/daily (1053 g n-3 PUFAs/day) for 3 weeks. Skin microvascular blood flow in response to iontophoresis of acetylcholine (AChID; endothelium-dependent) and sodium nitroprusside (SNPID; endothelium-independent) was assessed by laser Doppler flowmetry. Blood pressure (BP), body composition, body fluid status, serum lipid and free fatty acids profile, and inflammatory and endothelial activation markers were measured before and after respective dietary protocol. Results: Serum n-3 PUFAs concentration significantly increased, AChID significantly improved, and SNPID remained unchanged in n-3 PUFAs group, while none was changed in Control group. Interferon-γ (pro-inflammatory) significantly decreased and interleukin-10 (anti-inflammatory) significantly increased in n-3 PUFAs. BP, fat free mass, and total body water significantly decreased, while fat mass, interleukin-17A (pro-inflammatory), interleukin-10 and vascular endothelial growth factor A significantly increased in the Control group. Other measured parameters remained unchanged in both groups. Favorable anti-inflammatory properties of n-3 PUFAs consumption potentially contribute to the improvement of microvascular endothelium-dependent vasodilation in healthy individuals.
Objectives: We aimed to assess whether a 7-day high-salt (HS) diet affects endothelium-dependent and/or endothelium-independent microvascular function in the absence of changes in arterial blood ...pressure (BP), and to determine whether such microvascular changes are associated with changes in body composition and fluid status in healthy young humans. Materials and Methods: Fifty-three young healthy individuals (28 women and 25 men) were assigned to a 7-day low-salt diet (<3.5 g salt/day) followed by a 7-day HS diet (∼14 g salt/day). Skin microvascular blood flow in response to iontophoresis of acetylcholine (ACh) and sodium nitroprusside (SNP) was assessed by laser Doppler flowmetry, and BP, heart rate (HR), plasma renin activity (PRA), serum aldosterone, serum and 24 h-urine sodium, potassium, urea and creatinine levels, together with body composition and fluid status measurement with a 4-terminal portable impedance analyzer were measured before and after diet protocols. Results: BP, HR, body composition and fluid status were unchanged, and PRA and serum aldosterone level were significantly suppressed after HS diet. ACh-induced dilation (AChID) was significantly impaired, while SNP-induced dilation was not affected by HS diet. Impaired AChID and increased salt intake, as well as impaired AChID and suppressed renin-angiotensin system were significantly positively correlated. Changes in body composition and fluid status parameters were not associated with impaired AChID. Conclusion: 7-day HS diet impairs microvascular reactivity by affecting its endothelium-dependent vasodilation in young healthy individuals. Changes are independent of BP, body composition changes or fluid retention, but are the consequences of the unique effect of HS on endothelial function.
Carnosine is a dipeptide synthesized in the body from β-alanine and L-histidine. It is found in high concentrations in the brain, muscle, and gastrointestinal tissues of humans and is present in all ...vertebrates. Carnosine has a number of beneficial antioxidant properties. For example, carnosine scavenges reactive oxygen species (ROS) as well as alpha-beta unsaturated aldehydes created by peroxidation of fatty acid cell membranes during oxidative stress. Carnosine can oppose glycation, and it can chelate divalent metal ions. Carnosine alleviates diabetic nephropathy by protecting podocyte and mesangial cells, and can slow down aging. Its component, the amino acid beta-alanine, is particularly interesting as a dietary supplement for athletes because it increases muscle carnosine, and improves effectiveness of exercise and stimulation and contraction in muscles. Carnosine is widely used among athletes in the form of supplements, but rarely in the population of cardiovascular or diabetic patients. Much less is known, if any, about its potential use in enriched food. In the present review, we aimed to provide recent knowledge on carnosine properties and distribution, its metabolism (synthesis and degradation), and analytical methods for carnosine determination, since one of the difficulties is the measurement of carnosine concentration in human samples. Furthermore, the potential mechanisms of carnosine's biological effects in musculature, metabolism and on immunomodulation are discussed. Finally, this review provides a section on carnosine supplementation in the form of functional food and potential health benefits and up to the present, neglected clinical use of carnosine.
This randomized interventional study aimed to determine the effects of n-3 polyunsaturated fatty acids, selenium, vitamin E, and lutein supplementation in the form of enriched chicken egg consumption ...on microvascular endothelium-dependent vasodilation, oxidative stress, and microvascular response to an acute strenuous training session (ASTS) in competitive athletes. Thirty-one male athletes were assigned to a control (n = 17) or a Nutri4 group (n = 14) who consumed three regular or enriched chicken eggs per day, respectively, for 3 weeks. Significantly enhanced endothelium-dependent responses to vascular occlusion (PORH) and iontophoresis of acetylcholine (AChID) were observed in the Nutri4 group but not in the control group after egg consumption. Formation of peroxynitrite and hydrogen peroxide in peripheral blood mononuclear cells, as well as serum concentration of 8-iso prostaglandin F2α, decreased in the Nutri4 group while remaining unchanged in controls. PORH and AChID were reduced post-ASTS compared with pre-ASTS, both before and after the diets, in both groups. However, the range of PORH responsiveness to ASTS (ΔPORH) increased after consumption of enriched eggs. These results suggest that consumption of enriched chicken eggs has a beneficial effect on microvascular endothelium-dependent vasodilation and the reduction of oxidative stress levels in competitive athletes. Also, microvascular adaptation to the ASTS was improved after consumption of Nutri4 eggs.
This study aimed to examine whether the oral supplementation of vitamins C and E during a seven-day high salt diet (HS; ~14 g salt/day) prevents microvascular endothelial function impairment and ...changes oxidative status caused by HS diet in 51 (26 women and 25 men) young healthy individuals. Laser Doppler flowmetry measurements demonstrated that skin post-occlusive reactive hyperemia (PORH), and acetylcholine-induced dilation (AChID) were significantly impaired in the HS group, but not in HS+C+E group, while sodium nitroprusside-induced dilation remained unaffected by treatments. Serum oxidative stress markers: Thiobarbituric acid reactive substances (TBARS), 8-iso prostaglandin-F2α, and leukocytes' intracellular hydrogen peroxide (H
O
) production were significantly increased, while ferric-reducing ability of plasma (FRAP) and catalase concentrations were decreased in the HS group. All these parameters remained unaffected by vitamins supplementation. Matrix metalloproteinase 9, antioxidant enzymes Cu/Zn SOD and glutathione peroxidase 1, and leukocytes' intracellular superoxide production remained unchanged after the protocols in both HS and HS+C+E groups. Importantly, multiple regression analysis revealed that FRAP was the most powerful predictor of AChID, while PORH was strongly predicted by both FRAP and renin-angiotensin system activity. Hereby, we demonstrated that oxidative dis-balance has the pivotal role in HS diet-induced impairment of endothelial and microvascular function in healthy individuals which could be prevented by antioxidative vitamins consumption.
The present study aimed to determine the effect of n-3 polyunsaturated fatty acid (PUFA)-enriched hen eggs on microvascular vasodilation, microvascular responsiveness to a stress challenge and ...markers of oxidative stress in competitive athletes. Competitive athletes (n=23) were divided to a control group (n=9), who consumed three regular hens' eggs daily (249 mg n-3 PUFAs/d), and n-3 PUFAs group (n=14), who consumed three n-3 PUFA-enriched hen eggs daily (1,053 g n-3 PUFAs/d) for 3 weeks. Endothelium-dependent responses post-occlusive reactive hyperemia (PORH) and acetylcholine-induced dilation (AChID) and endothelium-independent responses sodium nitroprusside-induced dilation (SNPID) of skin microvascular blood flow were assessed by laser Doppler flowmetry in pre- and post-acute exhausting exercise (AEE) sessions. Blood pressure, serum lipid, free fatty acids profiles, and biomarkers of oxidative stress were measured before and after each dietary protocol. Consumption of serum n-3 PUFAs significantly decreased the n-6/n-3 ratio and enhanced PORH and AChID, but did not affect SNPID at rest. Furthermore, serum glutathione peroxidase and superoxide dismutase activities were significantly decreased in the n-3 PUFAs group but remained unchanged in the control group. In both groups, PORH, AChID, and SNP were significantly reduced post-AEE compared with pre-AEE, both before and after consumption of each diet. Only AChID responsiveness to AEE (ΔAChID) significantly increased following consumption of n-3 PUFAs. Overall, n-3 PUFAs supplementation as n-3 PUFA-enriched hen eggs enhanced microvascular endothelial function at rest and may contribute to adaptation to AEE in competitive athletes.
Ease.ml/snoopy in action Renggli, Cedric; Rimanic, Luka; Kolar, Luka ...
Proceedings of the VLDB Endowment,
08/2020, Letnik:
13, Številka:
12
Journal Article
Recenzirano
We demonstrate ease.ml/snoopy, a data analytics system that performs
feasibility analysis
for machine learning (ML) applications
before
they are developed. Given a performance target of an ML ...application (e.g., accuracy above 0.95), ease.ml/snoopy provides a decisive answer to ML developers regarding whether the target is achievable or not. We formulate the feasibility analysis problem as an instance of Bayes error estimation. That is, for a data (distribution) on which the ML application should be performed, ease.ml/snoopy provides an estimate of the Bayes error - the
minimum error rate
that can be achieved by
any
classifier. It is well-known that estimating the Bayes error is a notoriously hard task. In ease.ml/snoopy we explore and employ estimators based on the combination of (1) nearest neighbor (NN) classifiers and (2) pre-trained feature transformations. To the best of our knowledge, this is the first work on Bayes error estimation that combines (1) and (2). In today's cost-driven business world, feasibility of an ML project is an ideal piece of information for ML application developers - ease.ml/snoopy plays the role of a reliable "
consultant.
"
SHiFT Renggli, Cedric; Yao, Xiaozhe; Kolar, Luka ...
Proceedings of the VLDB Endowment,
10/2022, Letnik:
16, Številka:
2
Journal Article
Recenzirano
Odprti dostop
Transfer learning can be seen as a data- and compute-efficient alternative to training models from scratch. The emergence of rich model repositories, such as TensorFlow Hub, enables practitioners and ...researchers to unleash the potential of these models across a wide range of downstream tasks. As these repositories keep growing exponentially, efficiently selecting a good model for the task at hand becomes paramount. However, a single generic search strategy (e.g., taking the model with the highest linear classifier accuracy) does not lead to optimal model selection for diverse downstream tasks. In fact, using hybrid or mixed strategies can often be beneficial. Therefore, we propose SHiFT, the first downstream task-aware, flexible, and efficient model search engine for transfer learning. Users interface with SHiFT using the SHiFT-QL query language, which gives users the flexibility to customize their search criteria. We optimize SHiFT-QL queries using a cost-based decision maker and evaluate them on a wide rang of tasks. Motivated by the iterative nature of machine learning development, we further support efficient incremental executions of our queries, which requires a special implementation when jointly used with our optimizations.
In our experience of working with domain experts who are using today's AutoML systems, a common problem we encountered is what we call "unrealistic expectations" - when users are facing a very ...challenging task with a noisy data acquisition process, while being expected to achieve startlingly high accuracy with machine learning (ML). Many of these are predestined to fail from the beginning. In traditional software engineering, this problem is addressed via a feasibility study, an indispensable step before developing any software system. In this paper, we present Snoopy, with the goal of supporting data scientists and machine learning engineers performing a systematic and theoretically founded feasibility study before building ML applications. We approach this problem by estimating the irreducible error of the underlying task, also known as the Bayes error rate (BER), which stems from data quality issues in datasets used to train or evaluate ML models. We design a practical Bayes error estimator that is compared against baseline feasibility study candidates on 6 datasets (with additional real and synthetic noise of different levels) in computer vision and natural language processing. Furthermore, by including our systematic feasibility study with additional signals into the iterative label cleaning process, we demonstrate in end-to-end experiments how users are able to save substantial labeling time and monetary efforts.
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