Post-stroke depression is a common complication of stroke, with a high incidence rate and low recognition rate. Many patients do not receive effective intervention at the onset, which affects ...subsequent treatment outcomes. Post-stroke depression not only impacts the patient's mental well-being but also increases the risk of stroke recurrence and poor prognosis. Therefore, it has become a significant public health concern. Acupuncture has gained significant popularity in the treatment of post-stroke depression. However, there are inconsistent clinical research results regarding its efficacy and safety. This systematic review aims to gather and critically assess all available evidence regarding the effectiveness and safety of acupuncture in the treatment of post-stroke depression in patients.
We will conduct thorough searches for relevant studies in multiple electronic databases (PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure, VIP Database, Wan-fang Data and China Biomedical Database). Our search scope will encompass studies published from the inception of each database until September 2023. To evaluate the potential bias in all the included studies, we will adhere to the guidelines offered in the Cochrane Handbook. The total effective rate will be the primary outcome. To conduct a systematic review, we will employ RevMan 5.4 software.
This study will obtain efficacy and safety of acupuncture for the treatment of post-stroke depression.
The conclusions of this study will provide evidence-based perspectives that can guide clinical decision-making regarding the practicality and recommended timing of using acupuncture to treat post-stroke depression. Furthermore, this study will help advance the clinical application of acupuncture treatment for post-stroke depression and enhance its efficacy while ensuring patient safety.
This paper explored how the government provides low-carbon subsidies for the manufacturers, retailers, and consumers in a secondary supply chain under cap-and-trade scheme. We calculated the best ...prices, emissions reductions, and the demands for common and low-carbon products when subsidizing each of the abovementioned market players. In particular, a comparative analysis of their equilibrium outcomes was made thereafter. The MATLAB simulation found that the optimal emissions reductions under the three subsidy modes were even and positively correlated to low-carbon subsidies, which, however, negatively correlated to the prices of both product types. Higher subsidies drove up demand for low-carbon products and dragged down that for common goods. But the prices of these products maintained the highest levels when consumers were subsidized; demand for common products was greater when subsidies went to retailers than to manufacturers or consumers, consequently generating the largest emissions and highest profits. When the subsidies were greater than Formula: see text, all three subsidy modes saw a drop in total carbon emissions. That being so, the government should offer proper subsidies before seeing energy-saving progress.
The effects of solution and aging treatments on the microstructure, mechanical properties and its anisotropy of hot extruded Al-Mg-Si plate were studied. The results showed that all specimens ...consisted of equiaxed grains, and the obvious grain growth occurred during high temperature solution. GP zones with different morphologies always existed in the aged specimens, and β″ phase was formed after 2 h aging and gradually grew up to around 100 nm in length. The {001} Cube and {011}〈100〉 Goss are the main textures. The fraction of Cube drops significantly after solution and aging treatments, while the variation tendency of Goss is opposite. The peak hardness of 115 HV and tensile strength of 314 MPa were achieved after aging for 20 h. The elongation gradually decreased with prolonging the aging time, and the fracture mechanism was transformed from dimple fracture to intergranular fracture. The specimen along 90° with the extrusion direction always exhibited higher r value. The solution and aging treatments played important roles on the plastic anisotropy, and the anisotropy can be overall improved after aging treatment.
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•GP zones with different morphologies always existed in the aged specimens.•The main textures were Cube and Goss, while their fractions varied during extrusion, solution and aging.•The dimple fracture was transformed to intergranular mode with prolonging of aging time.•Solution and aging played important roles on the anisotropy, and the anisotropy was improved after aging.
The sesquiterpene alkaloid dendrobine, widely recognized as the main active compound and a quality control standard of medicinal orchids in the Chinese Pharmacopoeia, demonstrates diverse biological ...functions. In this study, we engineered
as a chassis plant for the production of dendrobine through the screening and pyramiding of key biosynthesis genes. Initially, previously predicted upstream key genes in the methyl-D-erythritol 4-phosphate (MEP) pathway for dendrobine synthesis, including
(
),
(
),
(
), and
(
), and a few downstream post-modification genes, including
(
),
(
), and
(
), were chosen due to their deduced roles in enhancing dendrobine production. The seven genes (
) were then stacked and transiently expressed in the leaves of
, resulting in a dendrobine yield that was two-fold higher compared to that of the empty vector control (
). Further, RNA-seq analysis identified
(
) as a strong candidate with predicted functions in the post-modification processes of alkaloid biosynthesis. Overexpression of
increased dendrobine content by two-fold. Additionally, co-expression analysis of the differentially expressed genes (DEGs) by weighted gene co-expression network analysis (WGCNA) retrieved one regulatory transcription factor gene
. Overexpression of
increased dendrobine levels by more than two-fold in
. In short, this work provides an efficient strategy and prospective candidates for the genetic engineering of
to produce dendrobine, thereby improving its medicinal value.
Magnetically coupled rodless cylinders are widely used in the coordinate positioning of mechanical arms, electrostatic paintings, and other industrial applications. However, they exhibit strong ...nonlinear characteristics, which lead to low servo control accuracy. In this study, a mass-flow equation through the valve port was derived to improve the control performance, considering the characteristics of the dynamics and throttle-hole flow. Subsequently, a friction model combining static, viscous, and Coulomb friction with a zero-velocity interval was proposed. In addition, energy and dynamic models were set for the experimental investigation of the magnetically coupled rodless cylinder. A nonlinear mathematical model for the position of the magnetically coupled rodless cylinder was proposed. An incremental PID controller was designed for the magnetically coupled rodless cylinder to control this system, and the PID parameters were adjusted online using RBF neural network. The response results of the PID parameters based on the RBF neural network were compared with those of the traditional incremental PID control, which proved the superiority of the optimization control algorithm of the incremental PID parameters based on the RBF neural network servo control system. The experimental results of this model were compared with the simulation results. The average error between the established model and the actual system was 0.005175054 (m), which was approximately 2.588% of the total travel length, demonstrating the accuracy of the theoretical model.
The research on biomimetic robots, especially soft robots with flexible materials as the main structure, is constantly being explored. It integrates multi-disciplinary content, such as bionics, ...material science, mechatronics engineering, and control theory, and belongs to the cross-disciplinary field related to mechanical bionics and biological manufacturing. With the continuous development of various related disciplines, this area has become a hot research field. Particularly with the development of practical technologies such as 3D printing technology, shape memory alloy, piezoelectric materials, and hydrogels at the present stage, the functions and forms of soft robots are constantly being further developed, and a variety of new soft robots keep emerging. Soft robots, combined with their own materials or structural characteristics of large deformation, have almost unlimited degrees of freedom (DoF) compared with rigid robots, which also provide a more reliable structural basis for soft robots to adapt to the natural environment. Therefore, soft robots will have extremely strong adaptability in some special conditions. As a type of robot made of flexible materials, the changeable pose structure of soft robots is especially suitable for the large application environment of the ocean. Soft robots working underwater can better mimic the movement characteristics of marine life in the hope of achieving more complex underwater tasks. The main focus of this paper is to classify different types of underwater organisms according to their common motion modes, focusing on the achievements of some bionic mechanisms in different functional fields that have imitated various motion modes underwater in recent years (e.g., the underwater sucking glove, the underwater Gripper, and the self-powered soft robot). The development of various task types (e.g., grasping, adhesive, driving or swimming, and sensing functions) and mechanism realization forms of the underwater soft robot are described based on this article.
Nasopharyngeal carcinoma (NPC) is a highly metastatic cancer, frequently occurring in Southeast Asia and Southern China. Several microRNAs (miRNAs) have been shown to have an inhibitive effect on ...NPC, while the effect of miR-15a on NPC remains unclear. Thus, our study aimed to investigate
the potential effect of miR-15a on NPC cell proliferation, apoptosis, and possible functional mechanism. Human NPC CNE1 cells were transfected with miR-15a mimics, miR-15a inhibitors, or a control. Afterward, cell viability and apoptosis were assayed by using CCK-8, BrdU assay, and flow cytometry.
Moreover, Western blot was used to detect the expression changes of proliferation and apoptosis of related proteins. As a result, miR-15a overexpression significantly reduced cell proliferation (p < 0.01 or p < 0.001) and induced cell apoptosis
(p < 0.001), while miR-15a suppression got the opposite result for cell proliferation and apoptosis. In addition, miR-15a overexpression upregulated the protein levels of p27, GSK-3β, Bax, procaspase 3, and active caspase 3, whereas miR-15a suppression downregulated
these proteins. The protein level of p21 was not significantly regulated by miR-15a overexpression or suppression. These results indicated that miR-15a played a role for inhibition of proliferation and induction of apoptosis in CNE1 cells.
Abstract
Checkpoint suppressor 1 (CHES1), a transcriptional regulator, had been dysregulated in many types of malignancies including breast cancer, and its expression level is strongly associated ...with progression and prognosis of patients. However, the underlying regulatory mechanisms of CHES1 expression in the breast cancer and the effects of post-translational modifications (PTMs) on its functional performance remain to be fully investigated. Herein, we found that CHES1 had a high abundance in triple-negative breast cancer (TNBC) and its expression was tightly associated with malignant phenotype and poor outcomes of patients. Furthermore, we confirmed that CHES1 was an acetylated protein and its dynamic modification was mediated by p300 and HDAC1, and CHES1 acetylation enhanced its stability via decreasing its ubiquitination and degradation, which resulted in the high abundance of CHES1 in TNBC. RNA-seq and functional study revealed that CHES1 facilitated the activation of oncogenic genes and pathways leading to proliferation and metastasis of TNBC. Taken together, this research established a novel regulatory role of acetylation on the stability and activity of CHES1. The results demonstrate the significance of CHES1 acetylation and underlying mechanisms in the progression of TNBC, offering new potential candidate for molecular-targeted therapy in breast cancer.
Chitin is the second most abundant polysaccharide in nature after cellulose. The degradation products of chitin, amino-oligosaccharides, possess good biological activities and promising applications. ...Chitinase plays an important role in the degradation of chitin, and its highly efficient production has attracted wide attention. In this study, the Bacillus licheniformis-derived chitinase BlChiA was recombinantly expressed in Bacillus subtilis. The optimal promoter SpoVG and signal peptide YqxI were obtained by screening the endogenous promoters and signal peptides. The extracellular enzyme activity of the recombinant strain reached 1.96 U mL−1, 1.94-fold higher than that of the original, previously constructed strain. On this basis, mutant libraries were constructed using error-prone PCR, and mutants with enhanced activities were obtained using high-throughput activity assays. After combination, the mutant E336K/A472T exhibited 3.61-fold greater activity than the previous result, which represented the highest expression level of BlChiA in Bacillus subtilis. In addition, the mutant also exhibited greater acid adaptability. These results provided a foundation for further exploration of the function and industrial application of the enzyme.
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•This is the inaugural effort to efficiently express BlChiA in Bacillus subtilis.•The promoter SpoVG and signal peptide YqxI facilitated BlChiA expression.•The E336K/A472T mutant with enhanced activity was obtained by directed evolution.•The expression level of BlChiA was increased by 3.61 times.•The chitinase BlChiA activity represented the highest level reported to date.
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Photo-Fenton-like catalysis allows development of novel advanced oxidation technology with promising application in wastewater treatment. In this work, carbon dots (CDs) were ...intercalated between CuO nanoparticles and coralloid flower-like graphitic carbon nitride (g-C3N4) to fabricate a ternary CuO/CDs/g-C3N4 hybrid for synergetic visible-light-driven photo-Fenton-like oxidation. The CuO/CDs/g-C3N4 hybrid showed remarkable degradation efficiency towards recalcitrant organic contamination, excellent tolerance to realistic environmental conditions, exceptional stability and wide universality, declaring great potential for practical applications. •OH and •O2− radicals were demonstrated to be the primary contributors in the photo-Fenton-like system. Mechanism studies reveal dual charge transfer pathways in the Z-scheme CuO/g-C3N4 heterojunction assisted by interfacial electron transmission bridges of CDs, which can simultaneously boost the reduction of Cu2+ to Cu+ in the Fenton-like cycle and accelerate the Z-scheme electron flow from CuO to g-C3N4, leading to synergistic enhancement of the catalytic performance. This work would afford a feasible strategy to develop reinforced solar energy-assisted photo-Fenton-like catalysis systems for water remediation.
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