With the goal of providing a comprehensive, high-quality resource for both plant transcription factors (TFs) and their regulatory interactions with target genes, we upgraded plant TF database ...PlantTFDB to version 4.0 (http://planttfdb.cbi.pku.edu.cn/). In the new version, we identified 320 370 TFs from 165 species, presenting a more comprehensive genomic TF repertoires of green plants. Besides updating the pre-existing abundant functional and evolutionary annotation for identified TFs, we generated three new types of annotation which provide more directly clues to investigate functional mechanisms underlying: (i) a set of high-quality, non-redundant TF binding motifs derived from experiments; (ii) multiple types of regulatory elements identified from high-throughput sequencing data; (iii) regulatory interactions curated from literature and inferred by combining TF binding motifs and regulatory elements. In addition, we upgraded previous TF prediction server, and set up four novel tools for regulation prediction and functional enrichment analyses. Finally, we set up a novel companion portal PlantRegMap (http://plantregmap.cbi.pku.edu.cn) for users to access the regulation resource and analysis tools conveniently.
With advances in next-generation sequencing technologies, numerous novel transcripts in a large number of organisms have been identified. With the goal of fast, accurate assessment of the coding ...ability of RNA transcripts, we upgraded the coding potential calculator CPC1 to CPC2. CPC2 runs ∼1000 times faster than CPC1 and exhibits superior accuracy compared with CPC1, especially for long non-coding transcripts. Moreover, the model of CPC2 is species-neutral, making it feasible for ever-growing non-model organism transcriptomes. A mobile-friendly web server, as well as a downloadable standalone package, is freely available at http://cpc2.cbi.pku.edu.cn.
Extracellular vesicles (EVs), including exosomes and microvesicles, are present in a variety of bodily fluids, and the concentration of these sub-cellular vesicles and their associated biomarkers ...(proteins, nucleic acids, and lipids) can be used to aid clinical diagnosis. Although ultracentrifugation is commonly used for isolation of EVs, it is highly time-consuming, labor-intensive and instrument-dependent for both research laboratories and clinical settings. Here, we developed an integrated double-filtration microfluidic device that isolated and enriched EVs with a size range of 30-200 nm from urine, and subsequently quantified the EVs via a microchip ELISA. Our results showed that the concentration of urinary EVs was significantly elevated in bladder cancer patients (n = 16) compared to healthy controls (n = 8). Receiver operating characteristic (ROC) analysis demonstrated that this integrated EV double-filtration device had a sensitivity of 81.3% at a specificity of 90% (16 bladder cancer patients and 8 healthy controls). Thus, this integrated device has great potential to be used in conjunction with urine cytology and cystoscopy to improve clinical diagnosis of bladder cancer in clinics and at point-of-care (POC) settings.
Objective
The efficacy of single-incision plus one-port laparoscopic surgery (SILS + 1) versus conventional laparoscopic surgery (CLS) for colorectal cancer treatment remains unclear. This study ...compares the short-term and long-term outcomes of SILS + 1 and CLS using a high-quality systematic review and meta-analysis.
Method
Literature search followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, drawing from PubMed, Embase, Web of Science, and the Cochrane Library until December 10, 2023. Statistical analysis was conducted using RevMan and Stata.
Result
The review and meta-analysis included seven studies with 1740 colorectal cancer patients. Compared to CLS, SILS + 1 showed significant improvements in operation time (WMD = − 18.33,
P
< 0.00001), blood loss (WMD = − 21.31,
P
< 0.00001), incision length (WMD = − 2.07,
P
< 0.00001), time to first defecation (WMD = − 14.91,
P
= 0.009), time to oral intake (WMD = − 11.46,
P
= 0.04), and time to ambulation (WMD = − 11.52,
P
= 0.01). There were no significant differences in lymph node harvest, resection margins, complications, anastomotic leakage, hospital stay, disease-free survival, overall survival, and postoperative recurrence.
Conclusions
Compared to CLS, SILS + 1 demonstrates superiority in shortening the surgical incision and promoting postoperative recovery. SILS + 1 can provide a safe and feasible alternative to CLS.
Diabetic nephropathy (DN) is one of the most important complications of diabetes mellitus (DM) and has become the second cause of end-stage renal disease (ESRD). This study intends to investigate the ...molecular mechanism of increased mitochondrial fission in podocytes under the effect of high glucose (HG), and to preliminarily study the role of mitochondrial fission factor (MFF)-mediated mitochondrial fission in podocyte injury of DN. In vitro studies, we found that HG induced increased mitochondrial fission and podocyte damage. At the same time MFF mRNA and protein levels was increased, suggesting that MFF was transcriptional upregulated under HG conditions. Consistent with this, in vivo studies found that mitochondrial fission was also significantly increased in podocytes of diabetic nephropathy mice, and MFF expression was up-regulated. Therefore, our study proves that mitochondrial fission increases in podocytes under DM both in vitro and in vivo, and the up-regulation of MFF expression may be one of the reasons for the increase of mitochondrial fission. After inhibiting the expression of MFF, the survival rate of podocytes was significantly decreased under HG conditions, suggesting that MFF may play a protective role in podocyte injury in DN.
•High glucose stimulation caused podocyte damage and increased mitochondrial fission.•The expression of mitochondrial fission factor was significantly up-regulated after high glucose stimulation.•Mitochondrial fission factor was protective against high glucose-stimulated podocytes.
Abstract
Esophageal squamous cell carcinoma (ESCC) is one of the most life- and health-threatening malignant diseases worldwide, especially in China. Long noncoding RNAs (lncRNAs) have emerged as ...important regulators of tumorigenesis and tumor progression. However, the roles and mechanisms of lncRNAs in ESCC require further exploration. Here, in combination with a small interfering RNA (siRNA) library targeting specific lncRNAs, we performed MTS and Transwell assays to screen functional lncRNAs that were overexpressed in ESCC.
TMPO-AS1
expression was significantly upregulated in ESCC tumor samples, with higher
TMPO-AS1
expression positively correlated with shorter overall survival times. In vitro and in vivo functional experiments revealed that
TMPO-AS1
promotes the proliferation and metastasis of ESCC cells. Mechanistically,
TMPO-AS1
bound to fused in sarcoma (FUS) and recruited p300 to the
TMPO
promoter, forming biomolecular condensates in situ to activate
TMPO
transcription
in cis
by increasing the acetylation of histone H3 lysine 27 (H3K27ac). Targeting
TMPO-AS1
led to impaired ESCC tumor growth in a patient-derived xenograft (PDX) model. We found that
TMPO-AS1
is required for cell proliferation and metastasis in ESCC by promoting the expression of
TMPO
, and both
TMPO-AS1
and
TMPO
might be potential biomarkers and therapeutic targets in ESCC.
Re‐extracting environmentally transportable hexavalent uranium from wastewater produced by spent fuel reprocessing using the photocatalytic technology is a crucial strategy to avoid uranium pollution ...and recover nuclear fuel strategic resources. Here, we have designed S‐scheme 2D/0D C3N5/Fe2O3 heterojunction photocatalysts based on the built‐in electric field and the energy band bending theory, and have further revealed the immobilization process of hexavalent uranium conversion into relatively insoluble tetravalent uranium in terms of thermodynamics and kinetics. According to the results, the hexavalent uranium removal and recovery ratios in wastewater are as high as 93.38% and 83.58%, respectively. Besides, C3N5/Fe2O3 heterojunctions also exhibit satisfactory catalytic activity and selectivity even in the presence of excessive impurity cations (including Na+, K+, Ca2+, Mg2+, Sr2+, and Eu3+) or various organics (such as xylene, tributylphosphate, pyridine, tannic acid, citric acid, and oxalic acid). It is believed that this work can provide a potential opportunity for S‐scheme heterojunction photocatalysts to re‐enrich uranium from spent fuel wastewater.
Efficient recovery of uranium species from nuclear wastewater through S‐scheme C3N5/Fe2O3 heterojunctions.
Chinese marine materia medica (CMMM) is a vital part of traditional Chinese medicine (TCM). Compared with terrestrial TCM, CMMM, derived from specific marine habitats, possesses peculiar chemical ...components with unique structures reflecting as potent pharmacological activities, distinct drug properties and functions. Nowadays, CMMM appears to be especially effective in treating such difficult diseases as cancers, diabetes, cardio-cerebrovascular diseases, immunodeficiency diseases and senile dementia, and therefore has become an important medicinal resource for the research and development of new drugs. In recent years, such development has attracted wide attention in the field of medicine. In this study, the CMMM resources in China were systematically investigated and evaluated. It was found that the historic experiences of Chinese people using CMMM have continuously accumulated over a period of more than 3600 years, and that the achievements of the research on modern CMMM are especially outstanding. By June 2015, 725 kinds of CMMMs from Chinese coastal sea areas have been identified and recorded, covering 1552 organisms and minerals. More than 3100 traditional prescriptions containing CMMMs have been imparted and inherited. However, the number of CMMMs is less than the 8188 terrestrial TCMs, from more than 12,100 medicinal terrestrial plants, animals and minerals. In the future, the research and development of CMMM should focus on the channel entries (TCM drug properties), compatibility, effective ingredients, acting mechanisms, drug metabolism and quality standard. This study reveals the high potential of CMMM development.
This study aims to develop new nanoformulations of EGFR T790M targeted inhibitor AZD9291 and paclitaxel (PTX) for combination therapy of lung cancer.
We prepared and characterized PTX- and ...AZD9291-loaded disulfide cross-linking micelles (DCMs), and evaluate their combination effect and toxicity in vitro and in lung cancer-bearing mice.
Drug-loaded DCMs were relatively small in size, and possessed glutathione-responsive drug release. The combination of PTX-DCMs and AZD92921-DCMs exhibited strong synergistic effects in both cell line and in vivo without additional toxicity. Molecular studies demonstrated the synergistic modification in both IKB-α/NF-κB/Bcl-2 and EGFR/Akt pathways.
The combination of DCM-loaded AZD9291 and PTX could potentially offer more effective and less toxicity treatment options for lung cancer patients.
The acoustic environments of Han Chinese Buddhist temples have long played an important role in the development of Buddhism. This study explored the effects of layouts and spatial elements of Han ...Chinese Buddhist temples on courtyard sound fields. First, sound fields of three traditional Han Chinese courtyards were measured, and results were compared with sound field simulations to determine the appropriate acoustic and software parameter setting for ancient building materials in the context of sound field simulation. Next, a sound field model for standard forms of Han Chinese Buddhist temples was built and analysed. Results indicate that in traditional Buddhist temples, spatial elements—such as the height and sound absorption coefficient of temple courtyard walls, position of courtyard partition walls, and the position and height of bell towers—could significantly affect the sound pressure level (SPL), reverberation time (RT), and musical clarity (C80) of each courtyard. However, enclosure materials, such as those used in roofs, on the ground, and in windows of Han Chinese Buddhist temples, had relatively small effects on temple courtyard sound fields.
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