In this review, an overview was given of the mutual interactions between nematodes and fungi of the genus Trichoderma sp. due to the potential of these fungi to protect plant roots from ...plant-parasitic nematodes on the one hand and the influence of nematodes (fungivores) on the efficacy of the fungus on the other. In addition, an overview of the advantages of Trichoderma sp. for agricultural production was given. The basis of sustainable agricultural production is the healthy functioning of the soil ecosystem. The diversity of organisms—bacteria, protozoa, algae, metazoans (nematodes) and fungi—improves the quality and performance of the soil by maintaining biological productivity. Root exudates in the rhizosphere support microbial communities that play a key role in regulating the dynamics of organic matter decomposition and the availability of plant nutrients. The microbial activity of organisms in the soil is interconnected and interacts to form a soil food web that reflects the condition, function and health of the soil. The energy in food webs flows through trophic chains of consumers, which are divided into energy channels. Root, bacterial and fungal channels increase soil biomass, carbon (C) and energy flow through the soil food web. The structure of the nematode community is an effective tool for the biological assessment of soil quality. This is due to a number of characteristics that nematodes have, including the following: a great diversity of species, the possibility of subdivision according to different criteria such as trophic groups and c-p groups, the duration of reproduction, the ease of sampling, the identification of genera and preservation, etc. Nematodes are involved in various ecological functions in the soil, of which the interaction between them and fungi is based on antagonism or mutualism, which is the basis for a better understanding of their impact on the ecosystem. Fungi of the genus Trichoderma sp. are successful colonizers of all habitats, secondary opportunists and fast growing.
Maslina (Olea europaea L.) je mediteranska voćna kultura koja zahtjeva navodnjavanje kako bi se postigao optimalni prinos i kvaliteta. Prakse navodnjavanja i štete od maslininog svrdlaša (Rhodocyrtus ...cribripennis (Desbrochers, 1869.)),štetnika koji napada plod, mogu biti bitan čimbenik pri određivanju stupanja zrelosti, jednog od glavnih indikatora kakvoće ploda. Cilj rada je utvrditi kako različita praksa, pri određivanju količine i učestalosti obroka navodnjavanja, uz prisutnost svrdlaša, utječe na indeks zrelosti masline, sorte 'Coratina'. Istraživanje je provedeno na 24 stabla masline na dvije lokacije u Zadarskoj županiji: Žman (Dugi otok) i Novigrad. Navodnjavanje je provedeno sustavom kap na kap sa četiri varijante u tri ponavljanja: K(0%) samo kišenje, T1(PP) proizvođač određivao obroke prema iskustvu, T2(SAN) obroci određivani obzirom na evapotranspiraciju i fenofazu razvoja, s do 80% poljskog kapaciteta tla i T3(100%) dodavanje vode do 100% od izračunate evapotranspiracije. Analizom tla određen je poljski kapacitet, a evapotranspiracija je očitavana s meteopostaje Pinova™. Berbe su obavljene u listopadu 2020. i 2021. Za određivanje štete od svrdlaša te indeksa zrelosti ubrano je 100 plodova po uzorku. Obradom podataka jednosmjernom analizom varijance (ANOVA) i povratnim testom (Tukey), utvrđen je statistički značajan utjecaj praksi navodnjavanja na indeks zrelosti ploda masline po tretmanima, godinama i lokacijama, jednostruko i u međuovisnosti. Nije ustanovljen utjecaj između prisutnosti svrdlaša i indeksa zrelosti.
This study evaluated the use of fluctuating asymmetry (FA) of shape, as a bio-indicator developmental stability (DS) in multiple populations of two different agricultural productions a) perennial ...(orchard) and b) annual (arable) crops on the carabid beetle Pterostichus melas melas (Creutzer, 1799) morphology. Shape variation and FA levels were estimated using geometric morphometrics. The results obtained using geometric morphometric analyses such as regressions (FA scores vs Shape) and partial least squares showed that carabids that inhabited the perennial agro-ecosystem seem to have adapted to the strong anthropogenic influence (i.e. IPM practices) at the phenotypic level, while the carabids inhabiting annual agro-ecosystems experience more unstable environments and their phenotypes seem to have been changed more recently. It was expected that phenotypes of the annual agro-ecosystems would be more variable than the long-established ones. Different IPM practices in agro-ecosystems generate different disturbance degrees in insect communities, and these effects can be successfully quantified by applying geometric morphometric techniques.
Poljoprivredna je proizvodnja od velike važnosti za prehranu ljudi i životinja. Budući da su potrebe za hranom svaki dan sve veće, a razina šteta se, ovisno o kulturi, umnogome ne mijenja, potrebno ...je naći nova rješenja. Svaki uzgoj bilja prati i njegova problematika ekonomski značajnih štetnih organizama i stresa uzrokovanog vanjskim i unutrašnjim čimbenicima. Takvi se problemi ne mogu više nadgledati samo ljudskim okom, pogotovu na većim parcelama, pa se rješenja traže u područjima precizne poljoprivrede. Suvremene tehnike uzimanja i obrade vizualnih podataka usmjerene su prema automatskom analiziranju usjeva preko slika i tako uvelike skraćuju vrijeme i rad inače potreban za otkrivanje prve pojave štetnih organizama i abiotskog stresa. Tehnike analize slika omogućuju lakše određivanje stupnja razvoja štetnog organizma (štetnika, biljnih bolesti, korova), klasifikaciju biljaka na zdrave i zaražene te otkrivanje biljnog stresa. Cilj je ovih analiza provjeriti zdravstveno stanje većeg broja biljaka u kraćem vremenu te predvidjeti mogućnost razvoja štetnog organizma i stresa bilja. U tu se svrhu danas koristi umjetna inteligencija kao najbolje rješenje. Ove tehnike i metode moraju biti brže, bolje i ekonomičnije od ljudskog rada. Rad rasvjetljuje nove spoznaje u području istraživanja i primjena tehnika detekcije štetnih organizama i stresa biljaka obradom vizualnih podataka usmjerenih kao alat za pomoć agronomu te pregledom dostupnih istraživanja u fitomedicini.
Nužna obrana jedan je od najstarijih oblika samopomoći te je, kao takva, često objekt doktrinarnih razmatranja. Općenito se smatra da napadna bilo koje zaštićeno dobro daje pravo na nužnu obranu. Ne ...zahtijeva se razmjernost između napadnutog dobra i dobra koje sepovreduje u obrani, lako hrvatsko zakonodavstvo, u skladu sa suvremenom doktrinom, prihvaća teoriju jednakovrijednosti ljudskih prava prema kojoj se ona ne mogu graduirati po svojem intenzitetu već je, u svakom pojedinom slučaju u kojem ona dolaze u koliziju, potrebno konkretno ocijeniti pretežnost interesa, još uvijek se ponekad nailazi na shvaćanja koja odriču časti i ugledu biti objektom nužne obrane. Cilj ovog rada je dokazati postojanje prava, odnosno moguće situacije nužne obrane u slučaju napada na čast i ugled čovjeka, kao i razraditi pretpostavke pod kojima je ona primjenjiva u okviru hrvatskog kaznenopravnog sustava.
A type of management and the use of pesticides in arable land may negatively affect a range of soil biota and thus their food webs important for ecosystem functioning. By analysing trophic ...interactions we could reveal the extent of potential benefits that certain organisms can provide in biocontrol and maintaining healthy ecosystems. To evaluate the role of predatory arthropods within olive orchards and vineyards under Integrated Pest Management (IPM) and Ecological Pest Management (EPM) we collected carabid beetles together with other dominant predatory arthropods in the field (e.g. ladybugs, antlions, spiders, centipedes) and subdued the individuals to molecular gut content analyses using NGS. DNA metabarcoding diet analysis approach allowed detecting a wide variety of taxa from gut contents of the predators. In addition, using ICP-MS and LC-MS/MS we quantified Cu, pesticides and its residues in soil and animals representing different trophic guilds. Since concentrations of some toxic compounds detected in carabids body were negatively correlated with those in the soil, we aim to identify a potential vector for possible transfer of toxicants to general predators via predation. The results contribute to the risk assessment of proliferation of detected chemical compounds including copper in the ecosystem and to the knowledge on the overall field sustainability of predatory invertebrates to maximize their role in pest control. The study was conducted under the project activity of HRZZ – Mediteratri.
Several species of the genus Fusarium can cause apple fruit to rot while stored. Since Fusarium taxonomy is very complex and has constantly been revised and updated over the last years, the aim of ...this study was to identify Fusarium species from rotten apples, based on combined morphological characteristics and molecular data. We identified 32 Fusarium isolates from rotten apple fruit of cultivars Golden Delicious, Jonagold, Idared, and Pink Lady, stored in Ultra Low Oxygen (ULO) conditions. Fusarium rot was detected in 9.4 % to 33.2 % of naturally infected apples, depending on the cultivar. The symptoms were similar in all four cultivars: a soft circular brown necrosis of different extent, with or without visible sporulation. Fusarium species were identified by the morphology of cultures grown on potato-dextrose agar (PDA) and carnation leaf agar (CLA). Twenty one isolates were identified as Fusarium avenaceum and confirmed as such with polymerase chain reaction (PCR) using specific primer pair FA-ITSF and FA-ITSR. F. pseudograminearum,F. semitectum, F. crookwellense, and F. compactum were identified by morphological characteristics. F.avenaceum can produce several mycotoxins and its dominance in Fusarium rot points to the risk of mycotoxin contamination of apple fruit juices and other products for human consumption. Pathogenicity tests showed typical symptoms of Fusarium rot in most of the inoculated wounded apple fruits. In this respect Fusarium avenaceum, as the dominant cause of Fusarium rot in stored apple fruits is a typical wound parasite.
Lisne uši (Insecta: Aphidoidea) pripadaju jednoj od najvažnijih skupina poljoprivrednih štetnika širom svijeta. Često uzrokuju ozbiljne, ekonomske probleme na različitim poljoprivrednim kulturama. ...Ponekad, zbog pojave rezistentnosti ovih kukaca na konvencionalna sredstva za zaštitu bilja zaštita je jako složena. Za razliku od kemijskih sredstava biljni ekstrakti pokazuju pozitivni učinak na okoliš, biološku raznolikost i ljudsko zdravlje. Cilj ovog istraživanja bio je odrediti učinkovitost različitih koncentracija vodenog i alkoholnog ekstrakta autohtonog (lokalni sortiment) i uvoznog češnjaka (Alium sativum L.) na šljivinu zelenu uš (Hyalopterus pruni Geoffr.). Pokus je obavljen u laboratorijskim uvjetima korištenjem Petrijevih posuda prethodno obloženih filter papirom na koji su postavljene lisne uši. U svaku Petrijevu posudu postavljeno je 15 jedinki. Vodeni i alkoholni ekstrakti su razrijeđeni u tri koncentracije (100%, 50% i 25%). Smrtnost kukaca je kontrolirana nakon 24 h i 48 h. Za izračun i korekciju mortaliteta korištena je Abott-ova formula. Statistički značajna razlika je uočena između tretmana i kontrole. Najveći učinak unutar 24 h je pokazao je 100 %-tni vodeni ekstrakt, kao i sve koncentracije alkoholnog ekstrakta. Najlošiji rezultat (9.99%) nakon 24 h je uočen kod 25%-tne vodene otopine. Nakon 48 h smrtnost je kod svih koncentracija bila veća od 75%.
Ionospheric delay is the major source of satellite positioning system performance degradation. Designers of satellite positioning systems attempt to mitigate the impact of the ionospheric delay by ...deployment of correction models. For instance, the American GPS utilises a global standard (Klobuchar) model, based on the assumption that the daily distribution of GPS ionospheric delay values follows a biased cosine curve during day-time, while during the night-time the GPS ionospheric delay remains constant. Providing a compromise between computational complexity and accuracy, the Klobuchar model is capable of correcting up to 70% of actual ionospheric delay, mainly during quiet space weather conditions. Unfortunately, it provides a very poor performance during severe space weather, geomagnetic and ionospheric disturbances. In addition, a global approach in Klobuchar model development did not take into account particularities of the local ionospheric conditions that can significantly contribute to the general GPS ionospheric delay. Current research activities worldwide are concentrating on a better understanding of the observed GPS ionospheric delay dynamics and the relation to local ionosphere conditions. Here we present the results of a study addressing daily GPS ionospheric delay dynamics observed at a Croatian coastal area of the northern Adriatic (position ϕ=45°N, λ=15°E) in the periods of quiet space weather in 2007. Daily sets of actual GPS ionospheric delay values were assumed to be the time series of composite signals, consisting of DC, cosine and residual components, respectively. Separate models have been developed that describe components of actual GPS ionospheric delay in the northern Adriatic for summer and winter, respectively. A special emphasis was given to the statistical description of the residual component of the daily distribution of GPS ionospheric delay, obtained by removing DC (bias) and cosine components from the composite GPS ionospheric delay. Future work will be focused on further evaluation and validation of a quiet space weather GPS ionospheric delay model for the northern Adriatic, transition to a non-Klobuchar model, and on research in local GPS ionospheric delay dynamics during disturbed and severe space weather conditions.
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