Markomanske vojne so bile največji in najobsežnejši oboroženi spopad rimske vojske in barbarskih plemen ob osrednjem Podonavju – Markomanov, Kvadov in Sarmatov skupaj s še drugimi plemeni. Vojne z ...germanskimi plemeni so svoje prve kali kazale že v obdobju Antonina Pija, miroljubnega predhodnika Marka Avrelija, ki je težnje uzurpatorjev skrbno umirjal s konsistentnim pacifizmom. V Panonijo so prihajala poslanstva germanskih kraljevin izza Donave, ki so vznemirjala z vestmi o premikih barbarskih ljudstev s severa. Trgovanje barbarskih ljudstev z rimskimi državljani na mejah rimske države in migracije v germanskem bazenu plemen so v pasu ob limesu predstavljali težnje le-teh po dodatnem habitatu znotraj imperija. Apijan piše, da so poslanstva teh plemen prišla celo v Rim k cesarju Antoninu Piju in ga prosila za sprejem v imperij. Kontinuirani pritiski so eskalirali najverjetneje v l. 166, ko je bil velik del rimske garnizije stacioniran na vzhodni meji v boju s Parti. Limes je bil v tem obdobju slabo zavarovan, saj so bile čete legij, stacioniranih od Rena do Donave, poslane na fronto s Parti. Kljub prizadevanju cesarskih namestnikov v obeh Panonijah, da bi preprečili izbruh vojne na utrjeni državni meji z Donavo, je barbarom uspel preboj čez limes in posledično prodor vse do Akvileje in Opitergija. Boleča zavest o ranljivosti Italije, ki jo je avgustejski mir (Pax Romana) uspaval za več rodov, je prinesla nagel zasuk v obrambi. Pričujoče besedilo pretresa dva glavna vira za dogodke med germansko-sarmatsko vojno in postavlja vprašanja o odsotnosti omemb pomembnega obrambnega sistema, t.i. »Pretenture Italije in Alp«.
The positive effects of honey bee venom on human health and the high market price are the main reasons for beekeepers' increasing interest in its production. However, until today there is no ...standardized technology for honey bee venom collection. Today's methods are mainly based on collectors placed at the hive entrance. The main problems with this method are the application of the collector, and the contamination that collected venom may contain which consequently reduces its quality. The disadvantages of this technology can at least partially be eliminated by the use of collectors placed inside the hive. Therefore, this study aimed to evaluate the effect of the collection method on the honey bee venom quality and quantity. The results of the study showed that significantly higher amounts of venom could be collected with collectors within the hive, while at the same time the quality of the venom, considering the melittin content, remained equal. Having in mind that honey bee venom collection has not had a significant impact on strength of the colonies (in comparison to control colonies that were not subjected to venom collection), collection technology with collectors placed inside the hive is a better option.
The effect of maternal smoking as a source of exposure to toxic metals Cd and Pb on superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity, metallothionein (MT), Cd, Pb, Cu, Fe, Mn, Se ...and Zn concentrations were assessed in maternal and umbilical cord blood and placenta in 74 healthy mother-newborn pairs after term delivery. Sparse discriminant analysis (SDA) was used to identify elements with the strongest impact on the SOD, GPx and MT in the measured compartments, which was then quantified by multiple regression analysis. SOD activity was lower in maternal and cord plasma, and higher in the placenta of smokers compared to non-smokers, whereas GPx activity and MT concentration did not differ between the groups. Although active smoking during pregnancy contributed to higher maternal Cd and Pb concentrations, its contribution to the variability of SOD, GPx or MT after control for other elements identified by SDA was not significant. However, an impaired balance in the antioxidant defence observed in the conditions of relatively low-to-moderate exposure levels to Cd and Pb could contribute to an increased susceptibility of offspring to oxidative stress and risk of disease development later in life. Further study on a larger number of subjects will help to better understand complex interactions between exposure to toxic elements and oxidative stress related to maternal cigarette smoking.
This study evaluates the interaction of toxic elements cadmium (Cd) and lead (Pb) due to exposure from cigarette smoking, essential elements, and steroidogenesis in the maternal–placental–fetal unit. ...In a cohort of 155 healthy, postpartum women with vaginal term deliveries in clinical hospitals in Zagreb, Croatia, samples of maternal blood/serum and urine, placental tissue, and umbilical cord blood/serum were collected at childbirth. The biomarkers determined were concentrations of Cd, Pb, iron (Fe), zinc (Zn), copper (Cu), and selenium (Se), and steroid hormones progesterone and estradiol in maternal and umbilical cord blood and the placenta. Three study groups were designated based on self-reported data on cigarette smoking habits and confirmed by urine cotinine levels: never smokers (n = 71), former smokers (n = 48), and active smokers (n = 36). Metal(loid)s, steroid hormones, urine cotinine, and creatinine levels were analyzed by ICP–MS, ELISA, GC–MS, and spectrophotometry. Cigarette smoking during pregnancy was associated with increased Cd levels in maternal, placental, and fetal compartments, Pb in the placenta, and with decreased Fe in the placenta. In active smokers, decreased progesterone and estradiol concentrations in cord blood serum were found, while sex steroid hormones did not change in either maternal serum or placenta. This study provides further evidence regarding toxic and essential metal(loid) interactions during prenatal life, and new data on sex steroid disruption in cord serum related to cigarette smoking. The results indicate that umbilical cord sex steroid levels may be a putative early marker of developmental origins of the future burden of disease related to harmful prenatal exposure to cigarette smoke.
Canine babesiosis is a rapidly spreading tick-borne disease in Europe, which entails protozoan parasites invading red blood cells. Small extracellular vesicles (EVs) (< 200 nm) were isolated from the ...serum of 15 healthy and 15 by Babesia canis naturally infected dogs aimed to distinguish EV characteristics and protein profiles. There were no significant differences (P = 0.05) observed in the mean sizes and concentrations of serum EVs between the healthy and canine babesiosis groups. Despite a higher number of Canis lupus proteins detected in EVs from serum of diseased dogs, there were no statistically significant differences (P < 0.05) in the number of protein IDs between the experimental groups. We successfully identified 211 Canis lupus proteins across both experimental groups, of which 147 Canis lupus proteins were validated as being EV-associated. This data set is accessible via the ProteomeXchange PXD047647. EVs isolated from serum of B. canis infected dogs were Cd9+, Cd63+, Cd81+, and Cd82+. Furthermore, 73 Canis lupus proteins were validated as EV-associated and specific for EVs isolated from serum of B. canis-infected dogs. These were predominantly membrane and cytosolic proteins, and innate and adaptive immune system-related proteins, especially those involved in adhesion and proteoglycan mechanisms like integrins. Enrichment was also observed for proteins involved in vascular and cellular responses, including signalling pathways such as VEGF, VEGFR, and the LKB1 network. When only blood-related sites of EV expression were evaluated, the origins of EV proteins were mostly cells of immune system. These were dendritic cells, neutrophils, B cells, monocytes and platelets. In general, proteins were enriched in pathways that collectively regulate various cellular processes, including immune responses, communication, signal transduction, membrane trafficking, and apoptosis. Serum EVs and their protein cargo may have an important role in both the invasion of B. canis and the host's response to the parasitic infection, nevertheless, additional experimental research is warranted. The overall count of identified EV proteins of parasitic origin, meeting cut off criteria of two peptides and 1 % FDR, was relatively low.
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•No significant differences in EV sizes and concentrations were observed between groups.•There were difference in the EV protein profile between groups.•73 proteins in B. canis-infected dogs were validated as EV-associated.•EV proteins were predominantly membrane and cytosolic proteins.•In canine babesiosis, identified EV proteins were part of immune system.
Due to its high nutritive value, royal jelly usage is increasing, both in human nutrition in native form and as bioactive component in other products (dietary supplements, medicines). The database ...and regulations on royal jelly characteristics are established in several countries, but not in Croatia. Physicochemical characteristics: moisture, protein content, pH value, total acidity, carbohydrate composition and 10-HDA content in 13 Croatian royal jelly samples were determined with the aim of getting insight to quality of royal jelly produced in Croatia. The obtained results showed that regarding 10-HDA content, one of the most important quality parameter, all samples fulfilled the international standard for royal jelly specifications. Moisture of three samples was higher than prescribed (69.5%, 76.3% and 72.0%, respectively) while one sample had slightly lower protein content than minimum 11% prescribed in international standard. Sucrose content in two royal jelly samples was higher than 3%. Statistically significant correlations were obtained between moisture and protein content, 10-HDA and total acidity as well as between fructose and glucose content. The results of this study will contribute to creation the database of Croatian royal jelly physicochemical characteristics and thus help in setting the royal jelly quality criteria at national level
Abstract Background and Study Design It is well documented that copper (Cu) blood levels are elevated in combination oral contraceptive (COC) users. The aim of this study was to establish the range ...of Cu increase in OC users compared to nonusers through a systematic literature overview and quantitative data analysis. Results Twenty-six articles were included in the meta-analysis. The increase in Cu level exponentially decreased in COC users over time, with a rapid decline through the 1960s and 1970s. After controlling for the publication year, use of COC increases the mean serum/plasma Cu level by 0.57 mg/L (95% confidence interval 0.49–0.66 mg/L). Conclusion COCs commonly raise serum Cu to levels between 1.5 and 2 mg/L, which are above reference levels. Although these levels are not considered toxic, there are suggestions that such Cu increase could be implicated in oxidative pathophysiological processes in the body. Further research on safety of COCs use, including oxidative-stress-related effects, is warranted.
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