Apoptosis is a genetically programmed cell death process with profound roles in development and disease. MicroRNAs modulate the expression of many proteins and are often deregulated in human ...diseases, such as cancer. C. elegans germ cells undergo apoptosis in response to genotoxic stress by the combined activities of the core apoptosis and MAPK pathways, but how their signalling thresholds are buffered is an open question. Here we show mir-35-42 miRNA family play a dual role in antagonizing both NDK-1, a positive regulator of MAPK signalling, and the BH3-only pro-apoptotic protein EGL-1 to regulate the magnitude of DNA damage-induced apoptosis in the C. elegans germline. We show that while miR-35 represses EGL-1 by promoting transcript degradation, repression of NDK-1 may be through sequestration of the transcript to inhibit translation. Importantly, dramatic increase in NDK-1 expression was observed in cells about to die. In the absence of miR-35, increased NDK-1 activity enhanced MAPK signalling that lead to significant increases in germ cell death. Our findings demonstrate that NDK-1 acts upstream of (or in parallel to) EGL-1, and that miR-35 targets both egl-1 and ndk-1 to fine-tune cell killing in response to genotoxic stress.
The neutron and its hypothetical mirror counterpart, a sterile state degenerate in mass, could spontaneously mix in a process much faster than the neutron β-decay. Two groups have performed a series ...of experiments in search of neutron – mirror-neutron (
n
−
n
′) oscillations. They reported no evidence, thereby setting stringent limits on the oscillation time τ
nn
′
. Later, these data sets have been further analyzed by Berezhiani et al.(2009–2017), and signals, compatible with
n
−
n
′ oscillations in the presence of mirror magnetic fields, have been reported. The Neutron Electric Dipole Moment Collaboration based at the Paul Scherrer Institute performed a new series of experiments to further test these signals. In this paper, we describe and motivate our choice of run configurations with an optimal filling time of 29 s, storage times of 180 s and 380 s, and applied magnetic fields of 10 μT and 20 μT. The choice of these run configurations ensures a reliable overlap in settings with the previous efforts and also improves the sensitivity to test the signals. We also elaborate on the technique of normalizing the neutron counts, making such a counting experiment at the ultra-cold neutron source at the Paul Scherrer Institute possible. Furthermore, the magnetic field characterization to meet the requirements of this
n
−
n
′ oscillation search is demonstrated. Finally, we show that this effort has a statistical sensitivity to
n
−
n
′ oscillations comparable to the current leading constraints for
B
′ = 0.
We report on the strategy used to optimize the sensitivity of our search for a neutron electric dipole moment at the Paul Scherrer Institute. Measurements were made upon ultracold neutrons stored ...within a single chamber at the heart of our apparatus. A mercury cohabiting magnetometer together with an array of cesium magnetometers were used to monitor the magnetic field, which was controlled and shaped by a series of precision field coils. In addition to details of the setup itself, we describe the chosen path to realize an appropriate balance between achieving the highest statistical sensitivity alongside the necessary control on systematic effects. The resulting irreducible sensitivity is better than 1 × 10
−26
e
cm. This contribution summarizes in a single coherent picture the results of the most recent publications of the collaboration.
We present the result of an experiment to measure the electric dipole moment (EDM) of the neutron at the Paul Scherrer Institute using Ramsey's method of separated oscillating magnetic fields with ...ultracold neutrons. Our measurement stands in the long history of EDM experiments probing physics violating time-reversal invariance. The salient features of this experiment were the use of a ^{199}Hg comagnetometer and an array of optically pumped cesium vapor magnetometers to cancel and correct for magnetic-field changes. The statistical analysis was performed on blinded datasets by two separate groups, while the estimation of systematic effects profited from an unprecedented knowledge of the magnetic field. The measured value of the neutron EDM is d_{n}=(0.0±1.1_{stat}±0.2_{sys})×10^{-26} e.cm.
The neutron gyromagnetic ratio has been measured relative to that of the 199Hg atom with an uncertainty of 0.8 ppm. We employed an apparatus where ultracold neutrons and mercury atoms are stored in ...the same volume and report the result γn/γHg=3.8424574(30).
We present for the first time a detailed and comprehensive analysis of the experimental results that set the current world sensitivity limit on the magnitude of the electric dipole moment (EDM) of ...the neutron. We have extended and enhanced our earlier analysis to include recent developments in the understanding of the effects of gravity in depolarizing ultracold neutrons (UCN); an improved calculation of the spectrum of the neutrons; and conservative estimates of other possible systematic errors, which are also shown to be consistent with more recent measurements undertaken with the apparatus. We obtain a net result of $d_\mathrm{n} = -0.21 \pm 1.82 \times10^{-26}$ $e$cm, which may be interpreted as a slightly revised upper limit on the magnitude of the EDM of $3.0 \times10^{-26}$ $e$cm (90% CL) or $ 3.6 \times10^{-26}$ $e$cm (95% CL). This paper is dedicated by the remaining authors to the memory of Prof. J. Michael Pendlebury.
We present the new spectrometer for the neutron electric dipole moment (nEDM) search at the Paul Scherrer Institute (PSI), called n2EDM. The setup is at room temperature in vacuum using ultracold ...neutrons. n2EDM features a large UCN double storage chamber design with neutron transport adapted to the PSI UCN source. The design builds on experience gained from the previous apparatus operated at PSI until 2017. An order of magnitude increase in sensitivity is calculated for the new baseline setup based on scalable results from the previous apparatus, and the UCN source performance achieved in 2016.
A validity test of E = m ; c(2) Jentschel, M; Krempel, J; Mutti, P
The European physical journal. ST, Special topics,
06/2009, Letnik:
172
Journal Article
Recenzirano
The comparison of mass and energy variation in a nuclear reaction allows an experimental verification of Einstein's energy - mass equivalence principle. Mass measurements are performed in a high ...precision Penning trap and yield values in unified atomic mass units. The energies of emitted gamma radiation are determined via Laue-diffraction with perfect crystals. The according values of the gamma ray wave lengths are expressed in units of the crystal lattice constant. The comparison of masses and wave lengths requires a conversion factor, which represents the unified atomic mass unit within the SI unit system. The latter is given by the molar Planck constant N(A)h, which itself is known via its relation to the fine structure constant. In the present paper we report on measurements carried out until 2003 with an uncertainty level of 4 ; 10(-7). We discuss the main limitations of these experiments and outline the possibilities for future measurements at the 10(-8) level. Such measurements would allow a direct representation of the unified atomic mass unit in terms of a Compton frequency and are of utmost importance for a future re-definition of the kilogram mass unit.
The design of the n2EDM experiment Ayres, N. J.; Ban, G.; Bienstman, L. ...
The European physical journal. C, Particles and fields,
2021/6, Letnik:
81, Številka:
6
Journal Article
Recenzirano
Odprti dostop
We present the design of a next-generation experiment, n2EDM, currently under construction at the ultracold neutron source at the Paul Scherrer Institute (PSI) with the aim of carrying out a ...high-precision search for an electric dipole moment of the neutron. The project builds on experience gained with the previous apparatus operated at PSI until 2017, and is expected to deliver an order of magnitude better sensitivity with provision for further substantial improvements. An overview is of the experimental method and setup is given, the sensitivity requirements for the apparatus are derived, and its technical design is described.
The comparison of gamma ray energies to mass variation in a nuclear reaction provides a mean to close the metrological triangle connecting together the Planck and the Avogadro constant by measuring ...the molar Planck constant. We suggest a scheme to experimentally validate the angle interferometers used in this experiment. Further, the results of this validation can be used to transform the gamma spectrometer in a universal lattice constant comparator.
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