Incubation of dialyzed Elapid venoms with the human plasma proteinase inhibitor, alpha 1-antichymotrypsin, resulted in enzymatic inactivation of the inhibitor by metalloproteinases in the crude ...venoms. Dendroaspis angusticeps venom exhibited the highest activity on alpha 1-antichymotrypsin. However, venoms from seven genera inactivated the inhibitor, indicating that the metalloproteinases responsible for the inactivation are widespread among snakes of the family Elapidae. Electrophoretic analysis revealed that intact alpha 1-antichymotrypsin (64,000 daltons) was converted to a 60,000 dalton inactive inhibitor. No stable complexes between alpha 1-antichymotrypsin and venom proteinases were observed, and no random proteolysis of the inhibitor occurred. The Elapid venoms showed little or no proteolytic activity on casein or hide powder azure, confirming observations from other laboratories. However, all venoms tested completely inactivated native alpha 1-antichymotrypsin by limited proteolysis.
The American Gear Manufacturers Association has issued a new product standard on the rating of mill and kiln gears. The base document for the new standard is also new and can lead to some confusion. ...The author attempts to clear up the difference in rating between the old and new girth gear standards. The maintenance of girth gears is described. Lubrication of girth gears is a key to their long life and is discussed.< >
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