Abstract
The use of rhizobacteria provide great benefits in terms of nitrogen supply, suppression of plant diseases, or production of vitamins and phytohormones that stimulate the plant growth. At ...the same time, cyanobacteria can photosynthesize, fix nitrogen, synthesize substances that stimulate rhizogenesis, plant aerial growth, or even suppose an extra supply of carbon usable by heterotrophic bacteria, as well as act as biological control agents, give them an enormous value as plant growth promoters. The present study focused on the in vitro establishment of consortia using heterotrophic bacteria and cyanobacteria and the determination of their effectiveness in the development of tomato seedlings. Microbial collection was composed of 3 cyanobacteria (SAB-M612 and SAB-B866 belonging to
Nostocaceae
Family) and GS (unidentified cyanobacterium) and two phosphate and potassium solubilizing heterotrophic bacteria (
Pseudomonas putida
-BIO175 and
Pantoea cypripedii
-BIO175). The results revealed the influence of the culture medium, incubation time and the microbial components of each consortium in determining their success as biofertilizers. In this work, the most compatible consortia were obtained by combining the SAB-B866 and GS cyanobacteria with either of the two heterotrophic bacteria. Cyanobacteria GS promoted the growth of both rhizobacteria in vitro (increasing logarithmic units when they grew together). While Cyanobacteria SAB-B866 together with both rhizobacteria stimulated the growth of tomato seedlings
in planta
, leading to greater aerial development of the treated seedlings. Parameters such as fresh weight and stem diameter stood out in the plants treated with the consortia (SAB-B866 and both bacteria) compared to the untreated plants, where the values doubled. However, the increase was more discrete for the parameters stem length and number of leaves. These results suggest that the artificial formulation of microbial consortia can have positive synergistic effects on plant growth, which is of enormous agro-biotechnological interest.
Flow cytometry has become a highly valuable method to monitor minimal residual disease (MRD) and evaluate the depth of complete response (CR) in bone marrow (BM) of multiple myeloma (MM) after ...therapy. However, current flow-MRD has lower sensitivity than molecular methods and lacks standardization. Here we report on a novel next generation flow (NGF) approach for highly sensitive and standardized MRD detection in MM. An optimized 2-tube 8-color antibody panel was constructed in five cycles of design-evaluation-redesign. In addition, a bulk-lysis procedure was established for acquisition of ⩾10
cells/sample, and novel software tools were constructed for automatic plasma cell gating. Multicenter evaluation of 110 follow-up BM from MM patients in very good partial response (VGPR) or CR showed a higher sensitivity for NGF-MRD vs conventional 8-color flow-MRD -MRD-positive rate of 47 vs 34% (P=0.003)-. Thus, 25% of patients classified as MRD-negative by conventional 8-color flow were MRD-positive by NGF, translating into a significantly longer progression-free survival for MRD-negative vs MRD-positive CR patients by NGF (75% progression-free survival not reached vs 7 months; P=0.02). This study establishes EuroFlow-based NGF as a highly sensitive, fully standardized approach for MRD detection in MM which overcomes the major limitations of conventional flow-MRD methods and is ready for implementation in routine diagnostics.
Cells exposed to extreme physicochemical or mechanical stimuli die in an uncontrollable manner, as a result of their immediate structural breakdown. Such an unavoidable variant of cellular demise is ...generally referred to as 'accidental cell death' (ACD). In most settings, however, cell death is initiated by a genetically encoded apparatus, correlating with the fact that its course can be altered by pharmacologic or genetic interventions. 'Regulated cell death' (RCD) can occur as part of physiologic programs or can be activated once adaptive responses to perturbations of the extracellular or intracellular microenvironment fail. The biochemical phenomena that accompany RCD may be harnessed to classify it into a few subtypes, which often (but not always) exhibit stereotyped morphologic features. Nonetheless, efficiently inhibiting the processes that are commonly thought to cause RCD, such as the activation of executioner caspases in the course of apoptosis, does not exert true cytoprotective effects in the mammalian system, but simply alters the kinetics of cellular demise as it shifts its morphologic and biochemical correlates. Conversely, bona fide cytoprotection can be achieved by inhibiting the transduction of lethal signals in the early phases of the process, when adaptive responses are still operational. Thus, the mechanisms that truly execute RCD may be less understood, less inhibitable and perhaps more homogeneous than previously thought. Here, the Nomenclature Committee on Cell Death formulates a set of recommendations to help scientists and researchers to discriminate between essential and accessory aspects of cell death.
Recent high angular resolution ( 40 mas) ALMA observations at 1.14 mm resolve a compact (R 200 au), flattened dust structure perpendicular to the HH 80-81 jet emanating from the GGD 27-MM1 high-mass ...protostar, making it a robust candidate for a true accretion disk. The jet-disk system (HH 80-81/GGD 27-MM1) resembles those found in association with low- and intermediate-mass protostars. We present radiative transfer models that fit the 1.14 mm ALMA dust image of this disk, which allow us to obtain its physical parameters and predict its density and temperature structure. Our results indicate that this accretion disk is compact (Rdisk 170 au) and massive ( 5 M ), at about 20% of the stellar mass of 20 M . We estimate the total dynamical mass of the star-disk system from the molecular line emission, finding a range between 21 and 30 M , which is consistent with our model. We fit the density and temperature structures found by our model with power-law functions. These results suggest that accretion disks around massive stars are more massive and hotter than their low-mass siblings, but they still are quite stable. We also compare the temperature distribution in the GGD 27-MM1 disk with that found in low- and intermediate-mass stars and discuss possible implications for the water snow line. We have also carried out a study of the distance based on Gaia DR2 data and the population of young stellar objects in this region and from the extinction maps. We conclude that the source distance is within 1.2 and 1.4 kpc, closer than what was derived in previous studies (1.7 kpc).
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•Intense turnover of organic matter was induced by inoculation of composting piles.•More simple compounds were released from polymeric fractions in inoculated piles.•Humification was ...more intense and earlier achieved in inoculated piles.•Inoculation clearly stimulated growth and activity of the composting microbiota.•Inoculation affected microbiota structure but its functionality remained unaltered.
Enhanced organic matter turnover was detected in lignocellulosic composting piles inoculated with microorganisms specifically capable of decomposing polymeric compounds. In comparison to uninoculated piles, the following results were obtained in the inoculated piles: degradation of hemicellulose, cellulose and lignin were 28%, 21% and 25% respectively higher. Total organic matter, total sugars and phenolic compounds also decreased more intensely. Greater amounts of soluble organic carbon, reducing sugars and soluble proteins were available to the composting microbiota. Recycling of organic to inorganic nitrogen was improved and humification was more intense and earlier attained. Microbial community structure was also affected by inoculation. It was initially thought that these effects were due to enzymatic capabilities of inoculants, however, microbial counts, especially those corresponding to functional groups, revealed that inoculation induced a true stimulation of microbial growth and activity in the entire composting microbiota which was actually responsible for all the beneficial effects reported here.
Cyanogen (NCCN) is the simplest member of the series of dicyanopolyynes. It has been hypothesized that this family of molecules can be important constituents of interstellar and circumstellar media, ...although the lack of a permanent electric dipole moment prevents its detection through radioastronomical techniques. Here we present the first solid evidence of the presence of cyanogen in interstellar clouds by detection of its protonated form toward the cold dark clouds TMC-1 and L483. Protonated cyanogen (NCCNH+) has been identified through the J = 5−4 and J = 10−9 rotational transitions using the 40 m radiotelescope of Yebes and the IRAM 30 m telescope. We derive beam-averaged column densities for NCCNH+ of (8.6 ± 4.4) × 1010 cm-2 in TMC-1 and (3.9 ± 1.8) × 1010 cm-2 in L483, which translate into fairly low fractional abundances relative to H2, in the range (1–10) × 10-12. The chemistry of protonated molecules in dark clouds is discussed, and it is found that, in general terms, the abundance ratio between the protonated and non-protonated forms of a molecule increases with increasing proton affinity. Our chemical model predicts an abundance ratio NCCNH+/NCCN of ~10-4, which implies that the abundance of cyanogen in dark clouds could be as high as (1–10) × 10-8 relative to H2, i.e., comparable to that of other abundant nitriles such as HCN, HNC, and HC3N.
The Little Ice Age in Iberian mountains Oliva, M.; Ruiz-Fernández, J.; Barriendos, M. ...
Earth-science reviews,
February 2018, 2018-02-00, Letnik:
177
Journal Article
Recenzirano
The Little Ice Age (LIA) is known as one of the coldest stages of the Holocene. Most records from the Northern Hemisphere show evidence of significantly colder conditions during the LIA, which in ...some cases had substantial socio-economic consequences. In this study we investigated the magnitude and timing of climate variability during the LIA in the mountains of the Iberian Peninsula, based on a wide range of natural records (including from glacial, periglacial, and lacustrine/peatland areas; fluvial/alluvial deposits; speleothems; and tree rings), historical documents, and early instrument data. The onset of the LIA commenced in approximately CE1300, and cold conditions with alternating moisture regimes persisted until approximately CE1850; the environmental responses ranged from rapid (e.g. tree rings) to delayed (e.g. glaciers). The colder climate of the LIA was accompanied by severe droughts, floods, and cold/heat waves that showed significant spatio-temporal variation across the Iberian mountains. Several phases within the LIA have been detected, including (a) 1300–1480: increasing cooling with moderate climate oscillations; (b) 1480–1570: relatively warmer conditions; (c) 1570–1620: gradual cooling; (d) 1620–1715: coldest climate period of the LIA, particularly during the Maunder Minimum, with temperatures approximately 2°C below those at present; (e) 1715–1760: warmer temperatures and a low frequency of extreme events; (f) 1760–1800: climate deterioration and more climate extremes (i.e. cold and heat waves, floods and droughts); (g) 1800–1850: highly variable climate conditions alternating with stability (1800–1815), extreme events (1815–1835), and a slight trend of warming associated with intense hydrometeorological events (1835–1850); (h) since 1850: a gradual staggered increase in temperature of approximately 1°C. Post-LIA warming has led to substantial changes in geo-ecological dynamics, mainly through shrinking of the spatial domain affected by cold climate processes.
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•Heterogeneity of composting process hinds the establishment of bioindicators.•Biodiversity and respirometric index affect phytostimulant power of compost.•Phenolic content and ...nitrification index could impact compost biodiversity.•Compost biodiversity is greater the lower the activity of xylanase.•Different raw materials evolve similar products by different bioestrategies.
Currently, the metagenomic study of the composting process has gained great importance since it has allowed the identification of the existence of microorganisms that, until now, had not been isolated during the process by traditional techniques. However, it is still complex to determine which bioindicators could reveal the degree of maturity and stability of a particular compost. Thereby, the main objective of this work was to demonstrate the possible correlation between traditional parameters of maturity and stability of compost, with other indicators of biodiversity in products highly heterogeneous from composting processes on an industrial scale.
The results demonstrated the enormous influence of the raw materials in characterizing the products obtained. Even so, important relationships were established between the Chao1 and Shannon indexes, and certain parameters related to the maturity, stability and toxicity of the samples, such as nitrification index, humification rate, phenolic content, germination index or oxygen consumption.
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