Aims
This study was performed to evaluate the efficacy of butanoic acid against bacterial pathogens including Acinetobacter baumannii and Staphylococcus pseudintermedius.
Methods and Results
...Vegetative bacteria were exposed to butanoic acid in vitro and log reduction was quantified using viable count assays. The maximum (8 and 9) log inactivation was determined by qualitatively assaying for growth/no‐growth after a 48‐h incubation (37°C). Membrane integrity after exposure to butanoic acid was determined by propidium iodide staining, scanning electron microscopy, membrane depolarization and inductively coupled plasma analysis. Cytosolic pH was measured by 5‐(6‐)carboxyfluorescein succinimidyl ester.
Conclusions
Inhibitory concentrations of butanoic acid ranged between 11 and 21 mmol l−1 for Gram‐positive and Gram‐negative species tested. The maximum log reduction of A. baumannii was achieved with a 10‐s exposure of 0·50 mol l−1 of butanoic acid. Staphylococcus pseudintermedius required 0·40 mol l−1 of butanoic acid to achieve the same level of reduction in the same time period. Inactivation was associated with membrane permeability and acidification of the cytosol.
Significance and Impact of the Study
Antibiotic resistance among bacterial pathogens necessitates the utilization of novel therapeutics for disinfection and biological control. These results may facilitate the development of butanoic acid as an effective agent against a broad‐spectrum of antibiotic‐resistant bacterial pathogens.
During the rapid mineralization in soil of sucralose (4-chloro-4-deoxy-alpha,D-galactopyranosyl-1, 6-dichloro-1,6-dideoxy-beta,D-fructofuranoside), a metabolic product was formed that appears to be ...the corresponding unsaturated aldehyde. During the slow and incomplete mineralization of sucralose in lake water, which was not increased by the addition of nitrogen and phosphorus, the same compound was produced. That product was further metabolized by microorganisms in lake water and soil. Mineralization was also slow in sewage under aerobic conditions, but organic products were not detected. Little or no CO2 was formed from the disaccharide in flooded soil or anaerobic sewage. Bacteria in culture did not use sucralose as a carbon source but did convert it to the presumed unsaturated aldehyde, 1.6-dichloro-1.6-dideoxy-D-fructose and possibly the uronic acid of sucralose. Sucralose carbon was not incorporated into cells of two sucralose-metabolizing bacteria or the microbial biomass of sewage or lake water. The chlorinated disaccharide was slowly metabolized by a galactose oxidase preparation. It is concluded that the chlorinated sugar is acted on microbiologically by cometabolism.
The CCube reconstruction algorithm for the SoLid experiment Abreu, Y.; Amhis, Y.; Arnold, L. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
September 2024, Letnik:
1066
Journal Article
Recenzirano
The SoLid experiment is a very-short-baseline experiment aimed at searching for nuclear-reactor-produced active-to-sterile antineutrino oscillations. The detection principle is based on the pairing ...of two types of solid scintillators: polyvinyl toluene and 6LiF:ZnS(Ag), which is a new technology used in this field of Physics. In addition to good neutron-gamma discrimination, this setup allows the detector to be highly segmented (the basic detection unit is a 5 cm side cube). High segmentation provides numerous advantages, including the precise location of inverse beta decay (IBD) products, the derivation of the antineutrino energy estimator based on the isolated positron energy, and a powerful background reduction tool based on the topological signature of the signal. Finally, the system is read out by a network of wavelength-shifting (WLS) fibres coupled to a photodetectors. This paper describes the design of the reconstruction algorithm that allows maximum use of the granularity of the detector. The goal of the algorithm is to convert the output of the optical-fibre readout to the list of the detection units from which it originated. This paper provides a performance comparison for three methods and concludes with a choice of the baseline approach for the experiment.
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