Purified L-cell colony-stimulating factor (CSF) was coupled to cyanogen-bromide-activated Sepharose and used to selectively fractionate antibodies to this factor. With the use of a simplified ...two-step washing and elution technique, there was 50 %–70% binding of the anti-CSF. with recovery of 60%-100% of the bound material. Both the native antiserum and purified anti-CSF fractions were inhibitory to murine granulocyte-macrophage colony formation. The purified antibodies contained only IgG and were reduced in protein concentration to 0.1 % of the serum IgG values. These fractions should prove useful tools for the study of granulocyte and macrophage differentiation.
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