Mosquitoes have adapted to various environmental conditions. Symbionts with mosquitoes impact this adaptation in different environments. In the field, mosquitoes could get exposed to antibiotics ...during their developmental period, which could reduce or eliminate their symbiotic microbes. However, the side effects of the antibiotics on the ovary and reproductive physiology of the Asian tiger mosquito, Aedes albopictus remains unknown. In this study, we investigated the effects of tetracycline and combinations of rifampicin and tetracycline at environmentally acceptable levels on the reproductive physiology of ovaries in Ae. albopictus. Rifampicin and tetracycline in combination reduced the hatching rate and fertility of Ae. albopictus compared to the untreated control group. These antibiotics induced histopathological damage and reactive oxygen species production in the ovaries. The combination of antibiotics decreased the expression of surface protein of Wolbachia (WSP) in Ae. albopictus. Additionally, the expression of Toll like receptor 2 (TLR2) and Myd88 were triggered by the combinations. The findings demonstrate the detrimental effects of antibiotics, particularly combinations of rifampicin and tetracycline, on the reproductive capacity of Ae. albopictus females.
We aimed to assess the trends in urinary tract infections (UTIs) and prognosis of patients with prostate cancer after radical prostatectomy (RP) and radiation therapy (RT) as definitive treatment ...options.
The data of patients diagnosed with prostate cancer between 2007 and 2016 were collected from the National Health Insurance Service database. The incidence of UTIs was evaluated in patients treated with RT, open/laparoscopic RP, and robot-assisted RP. The proportional hazard assumption test was performed using the scaled Schoenfeld residuals based on a multivariable Cox proportional hazard model. Kaplan-Meier analysis were performed to assess survival.
A total of 28,887 patients were treated with definitive treatment. In the acute phase (< 3 months), UTIs were more frequent in RP than in RT; in the chronic phase (> 12 months), UTIs were more frequent in RT than in RP. In the early follow-up period, the risk of UTIs was higher in the open/laparoscopic RP group (aHR, 1.63; 95% CI, 1.44-1.83; p < 0.001) and the robot-assisted RP group (aHR, 1.26; 95% CI, 1.11-1.43; p < 0.001), compared to the RT group. The robot-assisted RP group had a lower risk of UTIs than the open/laparoscopic RP group in the early (aHR, 0.77; 95% CI, 0.77-0.78; p < 0.001) and late (aHR, 0.90; 95% CI, 0.89-0.91; p < 0.001) follow-up periods. In patients with UTI, Charlson Comorbidity Index score, primary treatment, age at UTI diagnosis, type of UTI, hospitalization, and sepsis from UTI were risk factors for overall survival.
In patients treated with RP or RT, the incidence of UTIs was higher than that in the general population. RP posed a higher risk of UTIs than RT did in early follow-up period. Robot-assisted RP had a lower risk of UTIs than open/laparoscopic RP group in total period. UTI characteristics might be related to poor prognosis.
Melanogenesis is the biological process which the skin pigment melanin is synthesized to protect the skin against ultraviolet irradiation and other external stresses. Abnormal biology of melanocytes ...is closely associated with depigmented skin disorders such as vitiligo. In this study, we examined the effects of maclurin on melanogenesis and cytoprotection. Maclurin enhanced cellular tyrosinase activity as well as cellular melanin levels. We found that maclurin treatment increased the expression of microphthalmia-associated transcription factor (MITF), tyrosinase-related protein- (TRP-) 1, TRP-2, and tyrosinase. Mechanistically, maclurin promoted melanogenesis through cyclic adenosine monophosphate (cAMP) response element binding (CREB) protein-dependent upregulation of MITF. CREB activation was found to be mediated by p38 mitogen-activated protein kinase (MAPK) or cAMP-protein kinase A (PKA) signaling. In addition, maclurin-induced CREB phosphorylation was mediated through the activation of both the cAMP/PKA and the p38 MAPK signaling pathways. Maclurin-induced suppression of p44/42 MAPK activation also contributed to its melanogenic activity. Furthermore, maclurin showed protective effects against H2O2 treatment and UVB irradiation in human melanocytes. These findings indicate that the melanogenic effects of maclurin depend on increased MITF gene expression, which is mediated by the activation of both p38 MAPK/CREB and cAMP/PKA/CREB signaling. Our results thus suggest that maclurin could be useful as a protective agent against hypopigmented skin disorders.
Peroxiredoxin 2 (Prdx2), a thiol-specific peroxidase, has been reported to regulate proinflammatory responses, vascular remodeling, and global oxidative stress.
Although Prdx2 has been proposed to ...retard atherosclerosis development, no direct evidence and mechanisms have been reported.
We show that Prdx2 is highly expressed in endothelial and immune cells in atherosclerotic lesions and blocked the increase of endogenous H(2)O(2) by atherogenic stimulation. Deficiency of Prdx2 in apolipoprotein E-deficient (ApoE(-/-)) mice accelerated plaque formation with enhanced activation of p65, c-Jun, JNKs, and p38 mitogen-activated protein kinase; and these proatherogenic effects of Prdx2 deficiency were rescued by administration of the antioxidant ebselen. In bone marrow transplantation experiments, we found that Prdx2 has a major role in inhibiting atherogenic responses in both vascular and immune cells. Prdx2 deficiency resulted in increased expression of vascular adhesion molecule-1, intercellular adhesion molecule-1, and monocyte chemotactic protein-1, which led to increased immune cell adhesion and infiltration into the aortic intima. Compared with deficiency of glutathione peroxidase 1 or catalase, Prdx2 deficiency showed a severe predisposition to develop atherosclerosis.
Prdx2 is a specific peroxidase that inhibits atherogenic responses in vascular and inflammatory cells, and specific activation of Prdx2 may be an effective means of antiatherogenic therapy.
•The combustion and ash agglomeration characteristics of coal and BSW mixture were investigated.•Addition of BSW into the coal lowered the combustion temperature profiles during non-isothermal ...heating conditions.•Carbon and reagent gas reaction occurred on exterior and interior of the particle during char combustion.•BSW containing high phosphorus contents made a high agglomeration tendency and low melting mineral phase.
The co-combustion of sewage sludge and woody biomass is a key issue in coal power plants. Different combustion and ash behaviors of sewage sludge and woody biomass cause unpredictable operating concerns. In this study, the combustion and ash agglomeration behavior of blended fuel of sewage sludge and woody biomass (BSW) were investigated while coal co-combusted with it. Thermogravimetric analysis (TGA) revealed that adding a high amount of BSW into the coal lowered volatilization, ignition, and burn-out temperature. The char combustion reactivity of coal differed from that of BSW. The shrinking core model (SCM) and volumetric reaction model (VRM) were used to fit the char combustion reactivity of coal and BSW. In the case of ash agglomeration behavior, BSW addition led to increasing particle agglomeration at fouling temperatures. In particular, phosphorus composition influenced particle growth, which was verified using scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM–EDX) analysis. Furthermore, the ash mixture ratio of BSW and coal changed the intensity of the phosphorus-bearing mineral phase from X-ray diffraction (XRD) analysis, and finally influenced the melting temperature of the ash.
To investigate the influence of thermal cycling and mechanical loading (TCML) aging on fracture resistance and wear behavior of various chairside ...computer-aided-designed/computer-assisted-manufactured (CAD/CAM) premolar crowns cemented on standardized tooth abutments.
Eighty chairside CAD/CAM crowns were prepared using lithium disilicate (IPS e.max CAD; EM), zirconia-infiltrated lithium silicate (Celtra Duo; CD), polymer-infiltrated ceramic network (Vita Enamic; VE), and resin nanoceramics (Cerasmart; CS) (n = 20). The specimens were divided into two groups (n = 10). In one group, they were subjected to TCML: thermocycling (6000 cycles in distilled water at 5–55 °C) and mechanical loading (50 N for 1.2 × 106 cycles), while in control group they were stored in distilled water (37 °C for 24 h). The fracture load, height loss, and volume wear of the crowns were measured after TCML. Fractography was performed on fractured specimens. Data were analyzed using analysis of variance and multiple comparison tests (α=0.05).
The mean fracture loads of EM and CD were significantly higher than those of EC and CS (p<0.05). There was no significant change in the fracture load of any CAD/CAM crowns after TCML (p>0.05). CS exhibited a significantly higher volume wear than the other materials investigated. The wear tracts of all TCML crowns acted as failure origins during the fracture test.
The fracture resistance of glass-ceramic CAD/CAM crowns was significantly higher than that of resin composite crowns. A 5-year TCML aging did not affect the fracture resistance of CAD/CAM crowns investigated. However, TCML treatment produces a larger wear track in CS than in other materials.
Appropriate chairside CAD/CAM restorative material should be selected for successful clinical practice after considering the fracture and wear resistance of the crowns.
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Porous activated carbon as cathode of Li-ion capacitor was prepared through a twostep fabrication process using kenaf as biomass carbon precursor possible easily scaled-up for industrial production. ...The biomass-derived porous carbon shows an extremely high specific surface area of 2,719 m2 g−1 and relatively applicable pore volume of 1.6 m3 g−1 as well as well-developed carbon structure. As a result, it exhibits excellent capacitive performances with specific capacity of ~195 mA h g−1 at a current density of 0.1 A g−1, good rate capability at current rates ranging from 0.1 to 4 A g−1, and outstanding cycling stability in organic electrolyte.
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•Porous activated carbons were prepared through a two-step process using biomass carbon precursor.•The kAC-6 displays an extremely high specific surface area of 2,719 m2 g−1 and pore volume of 1.6 m3 g−1 with well-developed carbon structure.•The kAC-6 exhibits excellent capacitive performances as cathode of Li-ion capacitor with specific capacity of ~ 195 mA h g−1 at a current density of 0.1 A g−1, good rate capability at current rates ranging from 0.1 to 4 A g−1, and outstanding cycling stability.
On this work, we prepared the porous activated carbon as cathode of lithium-ion capacitor through two-step fabrication process using kenaf as biomass carbon precursor possible easily scaled-up for industrial production. The biomass-derived porous carbon shows an extremely high specific surface area of 2,719 m2 g−1 and relatively applicable pore volume of 1.6 m3 g−1 as well as well-developed carbon structure. As a result, it exhibits excellent lithium-ion capacitive performances with specific capacity of ~195 mA h g−1 at a current rate of 0.1 A g−1, good rate capability at current rates ranging from 0.1 to 4 A g−1, and outstanding cycling stability in organic electrolyte. These results suggest that the optimization of pore and carbon microstructure of biomass-derived carbon is contributed to outstanding electrochemical performance for improving the energy density of lithium-ion capacitor.
Melanogenesis is the process of production of melanin pigments that are responsible for the colors of skin, eye, and hair and provide protection from ultraviolet radiation. However, excessive levels ...of melanin formation cause hyperpigmentation disorders such as freckles, melasma, and age spots. Liver X receptors (LXR) are nuclear oxysterol receptors belonging to the family of ligand-activated transcription factors and physiological regulators of lipid and cholesterol metabolism. In the skin, activation of LXRs stimulates differentiation of keratinocytes and augments lipid synthesis in sebocytes. However, the function of LXRs in melanogenesis has not been clearly elucidated. In addition, although beauvericin, a well-known mycotoxin primarily isolated from several fungi, has various biological properties, its involvement in melanogenesis has not been reported. Therefore, in this study, we examined the effects of beauvericin on melanogenesis and its molecular mechanisms. Beauvericin decreased melanin content and tyrosinase activity without any cytotoxicity. Beauvericin also reduced protein levels of MITF, tyrosinase, TRP1, and TRP2. In addition, beauvericin suppressed cAMP-PKA-CREB signaling and upregulated expression of LXR-α, resulting in the suppression of p38 MAPK. Our results indicate that beauvericin attenuates melanogenesis by regulating both cAMP/PKA/CREB and LXR-α/p38 MAPK pathways, consequently leading to a reduction of melanin levels.
An effective method, involving time and frequency domains was developed to interpret seismic precursors by comparing groundwater-level fluctuations recorded immediately and long before the occurrence ...of a known earthquake. The proposed method, consisting of the pre-processing (3-point filtering, band-pass filtering, and spectrum analysis) and post-processing (weighted moving average method and histogram and spectrum analyses) stages, was applied to the groundwater-level time series measured at three monitoring wells on Jeju Island, South Korea, from 00:00 on 8 September 2016 to 00:00 on 22 September 2016. The Gyeongju earthquake (Mw 5.4) occurred at 20:32 on 12 September2016. The histogram analysis exhibited an accentuating bellshape as the total number of waveforms, including those caused by the earthquake, of the groundwater-level fluctuations increased. The weighted moving average analysis indicated that various abnormal waveforms with different periods occurred in the fluctuations approaching the occurrence of the earthquake. The periods of seismic precursors in the groundwater-level fluctuations were determined by spectrum analysis and varied among the monitoring wells. Seismic precursor responses attributable to the Gyeongju earthquake were identified at least 8 hours before the earthquake, and the method used in this study indicates its good potential to predict an impending earthquake.
To evaluate the formation of encrustation on double J stents (DJSs) using artificial urine.
In this study, a static urinary system containing artificial urine was created, and a total of 45 DJSs were ...used to evaluate the formation of encrustation. Three groups of 15 DJSs were tested for 4, 8, or 14 weeks. The formation of encrustation on the DJSs over the weeks was analyzed using methods including X-ray powder diffraction (XRD), inductively coupled plasma spectrophotometer (ICP), and scanning electron microscope (SEM). Statistical analysis and the uncertainty test were used for data analysis using R language.
The ICP analyzed the weight of the calcium and magnesium, which are the major components of urinary stones and encrustation, and showed that it was the heaviest at 14 weeks. Measurement of the area of encrustation on the outer surface of the DJSs revealed that the encrustation area at the bottom of the stent was greater than that at the top of the stent, regardless of the experimental period (proximal part: ≤ 41,099 µm
, distal part: ≤ 183,259 µm
). Encrustation occurred around the side holes of DJSs and became bigger over time to fill up the side holes.
Encrustation spots included the bottom zone of the DJS and around the side holes. These results indicate that the performance of DJSs would be improved by modifying the shape of DJSs located near the bladder and side holes.
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