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•Two step process coupled diluted acid hydrolysis with direct acidogenic fermentation.•Hydrolysis released up to 0.44 g carbohydrates per gram dried brewery spent grain.•Acidogenic ...fermentation of leachate by non-pretreated anaerobic granular sludge.•Highest volatile fatty acid production (16.9 g COD/L, 93.0 % CODsol) found at pH 6.0.
Brewery spent grain (BSG) is an industrial waste stream with large potential for biorefining purposes. This work evaluated the production of volatile fatty acids (VFAs) by a two-step process using BSG as renewable feedstock by combining a single direct hydrolysis step (without removing the acid or potential inhibiting compounds) with an acidogenic fermentation step of the carbohydrate rich leachate. For the first step, a thermal diluted acid hydrolysis was carried (20 min at 121 °C), using eighteen different combinations in terms of total solid (TS) of BSG (4, 7 and 10 % w/w) and H2SO4 (0.0, 0.5, 1.0, 1.5, 2.0 and 3.0 % v/v). The 7.0 % TS of BSG and 1.5 % of H2SO4 combination was the most efficient in terms of total carbohydrate recovery (0.44 g of total carbohydrates per gram of TS). For the second step, an acidogenic batch fermentation of the hydrolysate was performed using anaerobic granular sludge at five different pH conditions (uncontrolled pH from an initial pH 7.0, and constant pH controlled at 4.5, 5.0, 6.0 and 8.0). The highest VFAs concentration was obtained at pH 6.0 and reached 16.89 (± 1.33) g COD/L, composed of mainly (99.5–99.8 %) acetate and butyrate.
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•Production of Se-enriched microalgae in HRAP as feed supplement was assessed.•HRAP system showed a good wastewater treatment performance.•Microalgae have high ability to transform ...inorganic Se to organic Se.•49–63% of Se in the Se-enriched microalgae was bioaccessible for animals.•The nutritions of the Se-enriched microalgae were comparable to that of soybeans.
This study assessed the selenium (Se) removal efficiency of two pilot-scale high-rate algae ponds (HRAPs) treating domestic wastewater and investigated the production of Se-enriched microalgae as potential feed supplement. The HRAP-Se had an average Se, NH4+-N, total phosphorus and COD removal efficiency of, respectively, 43%, 93%, 77%, and 70%. Inorganic Se taken up by the microalgae was mainly (91%) transformed to selenoamino acids, and 49–63% of Se in the Se-enriched microalgae was bioaccessible for animals. The crude protein content (48%) of the microalgae was higher than that of soybeans, whereas the essential amino acid content was comparable. Selenium may induce the production of the polyunsaturated fatty acids omega-3 and omega-6 in microalgae. Overall, the production of Se-enriched microalgae in HRAPs may offer a promising alternative for upgrading low-value resources into high-value feed supplements, supporting the drive to a circular economy.
A sustainable biorefining and bioprocessing strategy was developed to produce edible-ulvan films and non-edible polyhydroxybutyrate films. The preparation of edible-ulvan films by crosslinking and ...plasticisation of ulvan with citric acid and xylitol was investigated using Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC) analysis. The edible ulvan film was tested for its gut-friendliness using Lactobacillus and Bifidobacterium spp. (yoghurt) and was shown to improve these gut-friendly microbiome's growth and simultaneously retarding the activity of pathogens like Escherchia coli and Staphylococcus aureus. Green macroalgal biomass refused after the extraction of ulvan was biologically processed by dark fermentation to produce a maximum of 3.48 (± 0.14) g/L of volatile fatty acids (VFAs). Aerobic processing of these VFAs using Cupriavidus necator cells produced 1.59 (± 0.12) g/L of biomass with 18.2 wt% polyhydroxybutyrate. The present study demonstrated the possibility of producing edible and non-edible packaging films using green macroalgal biomass as the sustainable feedstock.
Extracellular polymeric substances (EPS) were extracted from four anaerobic granular sludges with different procedures to study their involvement in biosorption of metallic elements. EPS extracts are ...composed of closely associated organic and mineral fractions. The EPS macromolecules (proteins, polysaccharides, humic-like substances, nucleic, and uronic acids) have functional groups potentially available for the binding of metallic elements. The acidic constants of these ionizable groups are: p
K
a1
(4–5) corresponding to the carboxyl groups; p
K
a2
(6–7) corresponding to the phosphoric groups; p
K
a3
(8–10) and p
K
a4
(≈10) corresponding to the phenolic, hydroxyl, and amino groups. The polarographic study confirms the higher affinity of the EPS to bind to lead than to cadmium. Moreover, the binding of these metallic compounds with the EPS is a mix of several sorption mechanisms including surface complexation, ion exchange, and flocculation. Inorganic elements were found as ions linked to organic molecules or as solid particles. The mineral fraction affects the binding properties of the EPS, as the presence of salts decreases the EPS binding ability. Calcite and apatite particles observed on SEM images of EPS extracts can also sorb metallic elements through ion exchange or surface complexation.
H2S removal performance by hollow fibre membrane bioreactors (HFMBs) was investigated for 271 days at ambient (20 ± 2 °C) temperature employing an inlet H2S concentrations up to 3600 ppmv and empty ...bed residence time (EBRT) of 187, 92 and 62 s. Different operating conditions including pH control (with or without), famine period, shock loads (4–72 h) and different biomass types (presence or absence of suspended biomass) were investigated. The H2S flux and mass-transfer coefficient were significantly higher for the biotic HFMBs (R1 and R2) compared to the abiotic control (R3) at all employed EBRTs. Significant differences in H2S removal efficiency (RE) and elimination capacity (EC) were noted for different inlet H2S concentrations, EBRTs, pH and biomass type. The HFMB achieved >99% RE at steady-state for biotic operation with an EC of 33.8, 30.0 and 30.9 g m−3 h−1 at an EBRT of 187, 92 and 62 s, respectively. Sulfate (92–93%) was the main sulfur species in the H2S bioconversion process. The HFMB showed a good resilience to shock loads and showed quick recovery (<24 h) after withdrawal of the shock loads. The HFMB had a critical loading rate of H2S about 135 g m−3 h−1 under transient-state.
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•The HFMB treated H2S up to 1255 ppmv under steady state with an elimination capacity of 30–34 g m−3 h−1•The critical loading rate of H2S was about 135 g m−3 h−1 under transient-state.•The mass-transfer coefficient was 16–35 times higher for the biotic process compared to abiotic process.•H2S removal efficiency dropped to 83% when pH was in the range of 4.4–5.5•SO42− generation increased from 63 to 87% when the EBRT was switched from 92 to 62 s.
Pyritic minerals generally occur in nature together with other trace metals as impurities, that can be released during the ore oxidation. To investigate the role of such impurities, the presence of ...copper (Cu(II)), arsenic (As(III)) and nickel (Ni(II)) during pyrite mediated autotrophic denitrification has been explored in this study at 30 °C with a specialized microbial community of denitrifiers as inoculum. The three metal(loid)s were supplemented at an initial concentration of 2, 5, and 7.5 ppm and only Cu(II) had an inhibitory effect on the autotrophic denitrification. The presence of As(III) and Ni(II) enhanced the nitrate removal efficiency with autotrophic denitrification rates between 3.3 7.5 ppm As(III) and 1.6 7.5 ppm Ni(II) times faster than the experiment without any metal(loid) supplementation. The Cu(II) batches, instead, decreased the denitrification kinetics with 16, 40 and 28% compared to the no-metal(loid) control for the 2, 5 and 7.5 ppm incubations, respectively. The kinetic study revealed that autotrophic denitrification with pyrite as electron donor, also with Cu(II) and Ni(II) additions, fits better a zero-order model, while the As(III) incubation followed first-order kinetic. The investigation of the extracellular polymeric substances content and composition showed more abundance of proteins, fulvic and humic acids in the metal(loid) exposed biomass.
Graphical Abstract
•Under CO2 sparging, T. neapolitana efficiently transforms starch to H2 and lactate.•Over 40% of acetate and exogenous CO2 recycled to produce lactate.•T. neapolitana could potentially valorize ...starch-rich waste without pretreatment.
This study investigated the feasibility of hydrogen (H2) and L-lactic acid production from starch under capnophilic lactic fermentation (CLF) conditions by using Thermotoga neapolitana. Batch experiments were performed in 120 mL serum bottles and a 3 L pH-controlled continuous stirred-tank reactors (CSTR) system with potato and wheat starch as the substrates. A H2 yield of 3.34 (±0.17) and 2.79 (±0.17) mol H2/mol of glucose eq. was achieved with, respectively, potato and wheat starch. In the presence of CO2, L-lactic acid production by the acetyl-CoA carboxylation was significantly higher for the potato starch (0.88 ± 0.39 mol lactic acid/mol glucose eq.) than wheat starch (0.33 ± 0.11 mol lactic acid/mol glucose eq.). A kinetic model was applied to simulate and predict the T. neapolitana metabolic profile and bioreactor performance under CLF conditions. The CLF-based starch fermentation suggests a new direction to biotransform agri-food waste into biofuels and valuable biochemicals.
•H2 and by-products production were investigated in solid state DF of food waste.•Effects of TS content and H2 partial pressure on substrate conversion were studied.•15% TS was found to be limiting ...for H2 production during solid state DF.
Production of biohydrogen and related metabolic by-products was investigated in Solid State Dark Fermentation (SSDF) of food waste (FW) and wheat straw (WS). The effect of the total solids (TS) content and H2 partial pressure (ppH2), two of the main operating factors of SSDF, were investigated. Batch tests with FW at 10, 15, 20, 25 and 30% TS showed considerable effects of the TS on metabolites distribution. H2 production was strongly inhibited for TS contents higher than 15% with a concomitant accumulation of lactic acid and a decrease in substrate conversion. Varying the ppH2 had no significant effect on the conversion products and overall degradation of FW and WS, suggesting that ppH2 was not the main limiting factor in SSDF. This study showed that the conversion of complex substrates by SSDF depends on the substrate type and is limited by the TS content.
The effect of temperature on selenium (Se) removal by upflow anaerobic sludge blanket (UASB) reactors treating selenate and nitrate containing wastewater was investigated by comparing the performance ...of a thermophilic (55 °C) versus a mesophilic (30 °C) UASB reactor. When only selenate (50 μM) was fed to the UASB reactors (pH 7.3; hydraulic retention time 8 h) with excess electron donor (lactate at 1.38 mM corresponding to an organic loading rate of 0.5 g COD L−1 d−1), the thermophilic UASB reactor achieved a higher total Se removal efficiency (94.4 ± 2.4%) than the mesophilic UASB reactor (82.0 ± 3.8%). When 5000 μM nitrate was further added to the influent, total Se removal was again better under thermophilic (70.1 ± 6.6%) when compared to mesophilic (43.6 ± 8.8%) conditions. The higher total effluent Se concentration in the mesophilic UASB reactor was due to the higher concentrations of biogenic elemental Se nanoparticles (BioSeNPs). The shape of the BioSeNPs observed in both UASB reactors was different: nanospheres and nanorods, respectively, in the mesophilic and thermophilic UASB reactors. Microbial community analysis showed the presence of selenate respirers as well as denitrifying microorganisms.
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•UASB reactor at 55 °C achieves a 10–15% higher total Se removal efficiency than at 30 °C.•BioSeNPs produced at 55 °C are nanorods, whereas nanospheres at 30 °C.•Adding NO3– to the feed decreases the Se removal efficiency.
The effects of voltage intensity on the nutrient removal performance and microbial community in the iron electrolysis-integrated aerobic granular sludge (AGS) system were investigated over a period ...of 15 weeks. Results revealed that the application outcomes of iron electrolysis for AGS systems relied on voltage intensity. When a constant voltage of 1.5 V was applied, the sludge granulation was most obviously accelerated with a specific growth rate of the sludge diameter of 0.078 day−1, and the removal efficiencies of total nitrogen (TN) and total phosphorus (TP) increased by 14.1% and 20.2%, respectively, compared to the control reactor (without the iron electrolysis-integration). Moreover, the AGS developed at different voltages included different microbial communities, whose shifts were driven by the Fe content and the average diameter of AGS. Both heterotrophic nitrifiers and mixotrophic denitrifiers were significantly enriched in the AGS developed at 1.5 V, which effectively enhanced TN removal. Together with the response of the functional genes involved in Fe, N, and P metabolism, the electrolytic iron-driven nutrient degradation pathway was further elaborated. Overall, this study clarified the optimum voltage condition when iron electrolysis was integrated into the AGS system, and revealed the enhancement mechanism of this coupling technology on nutrient removal during the treatment of low-strength municipal wastewater.
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•Nutrient removal of AGS was notably enhanced by iron electrolysis at 1.5 V.•Microbial community structure was shaped by Fe content and average diameter of AGS.•Heterotrophic nitrifiers and mixotrophic denitrifiers were enriched in AGS.•Variation in abundance of functional genes under iron electrolysis was revealed.•Pathway of N and P metabolism mediated by electrolytic ferrous was elaborated.
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