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•Legacy, short chain and emerging PFASs in residential soils of 89 cities were studied.•Serious pollution of PFASs occurred in Hunan and Eastern Coastal China.•F-53B and short-chain ...analogues already became the dominant PFAS pollutants.•GenX and FC-98 were detectable in residential soil with a relatively low level.
With the worldwide regulation of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS), the alternatives (short chain analogues and emerging per- and polyfluoroalkyl substances, PFASs) have gradually attracted global attention. This study analysed the replacing of legacy PFASs in China using PFASs data from residential soils, which might be good environmental indicators of their present usage. The total concentrations of 21 PFASs ranged from 244 to 13564 pg/g, and PFOA was the dominant compound among the studied PFASs, with a concentration of 354 ± 439 pg/g. Serious PFASs pollution in residential soils mainly occurred in Eastern Coastal China as a result of locally developed industry and economies. Weak but significant correlations were found between PFASs and environmental and socioeconomic factors, suggesting that various factors determine PFASs contamination in residential soils. The concentration and detection frequency (DF) of short-chain analogues (C < 8) (375 ± 509 pg/g and 100%), and F-53B (216 ± 306 pg/g and 98.9%) were higher than those for PFOS (193 ± 502 pg/g and 85.4%), indicating that these compounds have been widely used as PFOS alternatives and their consumption has already exceeded that of PFOS in China. In addition, GenX (the PFOA alternative) had a concentration and DF of 19.1 ± 104 pg/g and 40.5%, respectively. These values were much lower than those for PFOA (354 ± 439 pg/g and 96.6%), indicating GenX consumption is still limited at the national scale of China, despite its use as a PFOA replacement. Moreover, the low concentration and DF of FC-98 (2.31 ± 11.1 pg/g and 27.0%) indicate that its consumption might be negligible. Our study demonstrated that short chain analogues and emerging alternatives have become the dominant PFAS pollutants in Chinese residential soils, and further studies need to be conducted to understand their toxicity and environmental risks.
Previous studies on the pneumonia outbreak caused by the 2019 novel coronavirus disease (COVID-19) were based on information from the general population. Limited data are available for pregnant women ...with COVID-19 pneumonia. This study aimed to evaluate the clinical characteristics of COVID-19 in pregnancy and the intrauterine vertical transmission potential of COVID-19 infection.
Clinical records, laboratory results, and chest CT scans were retrospectively reviewed for nine pregnant women with laboratory-confirmed COVID-19 pneumonia (ie, with maternal throat swab samples that were positive for severe acute respiratory syndrome coronavirus 2 SARS-CoV-2) who were admitted to Zhongnan Hospital of Wuhan University, Wuhan, China, from Jan 20 to Jan 31, 2020. Evidence of intrauterine vertical transmission was assessed by testing for the presence of SARS-CoV-2 in amniotic fluid, cord blood, and neonatal throat swab samples. Breastmilk samples were also collected and tested from patients after the first lactation.
All nine patients had a caesarean section in their third trimester. Seven patients presented with a fever. Other symptoms, including cough (in four of nine patients), myalgia (in three), sore throat (in two), and malaise (in two), were also observed. Fetal distress was monitored in two cases. Five of nine patients had lymphopenia (<1·0 × 10⁹ cells per L). Three patients had increased aminotransferase concentrations. None of the patients developed severe COVID-19 pneumonia or died, as of Feb 4, 2020. Nine livebirths were recorded. No neonatal asphyxia was observed in newborn babies. All nine livebirths had a 1-min Apgar score of 8–9 and a 5-min Apgar score of 9–10. Amniotic fluid, cord blood, neonatal throat swab, and breastmilk samples from six patients were tested for SARS-CoV-2, and all samples tested negative for the virus.
The clinical characteristics of COVID-19 pneumonia in pregnant women were similar to those reported for non-pregnant adult patients who developed COVID-19 pneumonia. Findings from this small group of cases suggest that there is currently no evidence for intrauterine infection caused by vertical transmission in women who develop COVID-19 pneumonia in late pregnancy.
Hubei Science and Technology Plan, Wuhan University Medical Development Plan.
The concentrations and spatial occurrences of 17 legacy per- and polyfluoroalkyl substances (PFAS) and 4 emerging PFAS in the coastal water-dissolved phase, surface sediment phase and suspended ...particulate matter (SPM) in the coastal areas of Bohai Bay were investigated. In addition, the partition behaviors of PFAS in the water-SPM system and water-sediment system and the potential sources of PFAS in the marine environment were revealed. The total concentrations of PFAS (∑PFAS) in the water-dissolved phase, surface sediment and SPM were 20.5–684 ng/L, 2.69–25.0 ng/g dry weight (dw) and 4.39–527 ng/g dw, respectively. The level of PFAS contamination in the coastal areas of Shandong Province was higher than that in other areas. The average partition coefficients (log Kd) of PFAS in the water-SPM system and water-sediment system were 1.56–3.57 and 0.72–2.95, respectively. Long-chain PFAS and PFECHS (perfluoroethylcyclohexane sulfonate) have a higher log Kd than that of short-chain PFAS. PFAS with short carbon chains were mainly detected in the water-dissolved phase, but long-chain PFAS mainly occurred in the surface sediment and SPM phases. Source analysis based on the positive matrix factorization (PMF) model found that erosion inhibitor factories, aqueous film-forming foam factories, metal plating plants, fluoropolymer chemical manufacture and food contact materials were the main sources of PFAS in Bohai Bay. These results improved our understanding of the partitioning behavior and sources of PFAS in aquatic environments.
∑PFAS (ng/L) and composition profile in water dissolved phase in the coastal area of Bohai bay, China. Display omitted
•Occurrences and sources of PFAS in water, surface sediment and SPM of Bohai bay were investigated.•HFPO-DA and Cl-PFAESs were ubiquitous in water dissolved phase.•Short-chain PFAS were mostly presented in water, while long-chain PFAS prevailed in sediment and SPM.•AFFFs factories, metal plating plants, FP manufacture and food contact materials are the key contributing to PFAS.
The occurrence, partition behaviors and potential source of legacy and emerging PFAS in coastal areas were investigated.
Advances in CD247 Dexiu, Chen; Xianying, Lei; Yingchun, Hu ...
Scandinavian journal of immunology,
July 2022, Letnik:
96, Številka:
1
Journal Article
Recenzirano
CD247, which is also known as CD3ζ, CD3H, CD3Q, CD3Z, IMD25, T3Z and TCRZ, encodes CD3ζ protein, which is expressed primarily in natural killer (NK) and T cells. Since the discovery of the ζ peptide ...in 1986, it has been continuously investigated. In this paper, we review the composition, molecular mechanisms and regulatory factors of CD247 expression in T cells; and review the autoimmune diseases, tumours and inflammatory diseases associated with CD247, providing a detailed and comprehensive reference for further research on the mechanism of CD247 and related diseases.
Spacer acquisition step in CRISPR-Cas system involves the recognition and subsequent integration of protospacer by the Cas1-Cas2 complex in CRISPR-Cas systems. Here we report an anti-CRISPR protein, ...AcrVA5, and reveal the mechanisms by which it strongly inhibits protospacer integration. Our biochemical data shows that the integration by Cas1-Cas2 was abrogated in the presence of AcrVA5. AcrVA5 exhibits low binding affinity towards Cas2 and acetylates Cas2 at Lys
on the binding interface of the Cas2 and AcrVA5 N-terminal peptide complex to inhibit the Cas2-mediated endonuclease activity. Moreover, a detailed structural comparison between our crystal structure and homolog structure shows that binding of AcrVA5 to Cas2 causes steric hindrance to the neighboring protospacer resulting in the partial disassembly of the Cas1-Cas2 and protospacer complex, as demonstrated by electrophoretic mobility shift assay. Our study focuses on this mechanism of spacer acquisition inhibition and provides insights into the biology of CRISPR-Cas systems.
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•Contamination of MPs in Bohai Bay coast was at moderate level.•Total number of MPs in the sediments of Bohai Sea was 534 trillion.•Densities of some PE and PP particles were higher ...than that of seawater.•Density increasing rates were 7.4%–12.6% and 12.1%–17.5% for PE and PP, respectively.•Density increasing was caused by the co-contribution of biofouling and minerals.
Size, shape and color are the basic parameters of environmental microplastic (MPs). However, the density of microplastics which is an important parameter to influence the fate of MPs in the environment has not been measured. Meanwhile, the MPs characteristics from coastal sediments of Bohai Bay remain unclear. In this study, the concentrations, distribution and composition (shapes and sizes) of MPs were investigated. The average concentration of MPs in the coastal sediments of Bohai Bay was 192.3 ± 138.9 items/kg dw, which was at a moderate pollution level. The MPs with fiber shape (54.5%), small size (52.0%) and low density (PE and PP) were dominant. In addition, flotation experiment showed that most of PE and PP had the higher density compared with seawater. Digestion experiments suggested that the density change of MPs was caused by the co-contribution of biofouling and inorganic minerals. The density increasing rates of PE and PP were 7.4%–12.6% and 12.1%–17.5%, respectively. Finally, we also estimated that the total number of MPs in the sediments of Bohai Sea was 534 trillion, which were significantly higher than that in global ocean surface, suggesting that the coastal sediments could be the sink of MPs.
Perfluoroalkyl acids (PFAAs) are a group of emerging persistent organic pollutants (POPs), which have been ubiquitously detected in the environmental media. However, national scale investigations on ...their occurrence and distribution in drinking water are still insufficient. In this study, we detected the 17 priority PFAAs in drinking water from 79 cities of 31 provincial-level administrative regions throughout China, and investigated their occurrence and distribution. Additionally, we also analyzed the influencing factors on their profiles, such as the existence of industrial sources, socioeconomic factors (population density and GDP), and assessed levels of risk associated with contaminated drinking water. On the national scale, the sum concentrations of the 17 PFAAs (∑17PFAAs) in drinking water was in a range of 4.49–174.93 ng/L with a mean value of 35.13 ng/L. Among the 17 individual PFAAs, perfluorobutanoic acids (PFBA) was the most abundant individual PFAAs with the median concentration of 17.87 ng/L, followed by perfluorooctanoic acid (PFOA, 0.74 ng/L), perfluorononanoic acid (PFNA, 0.40 ng/L) and perfluorooctane sulfonic acid (PFOS, 0.25 ng/L). The geographic distribution characteristic of ∑17PFAAs in drinking water was in a descending order of Southwestern China (57.67 ng/L) > Eastern coastal China (32.85 ng/L) > Middle China (29.89 ng/L) > Northwestern China (28.49 ng/L) > Northeastern China (22.03 ng/L), and in general, the existence of the industrial sources could positively affect the contamination levels of PFAAs in drinking water. The pollution level of PFAAs in drinking water also varied among the three different city levels (medium-sized city > big city > town). In towns, the positive correlations were observed between the population density and the ∑17PFAAs (R2 = 0.45, p < 0.01), and the individual concentration of PFHxA, PFBS, and PFOA (p < 0.01). Moreover, besides PFAAs in Yunnan, Jiangsu, and Jiangxi, concentrations of related PFAAs in drinking water from 28 provinces were less than the suggested drinking water advisories. The relatively higher concentrations of PFAAs in Yunnan, Jiangsu, and Jiangxi suggest that further studies focusing on their sources and potential health risk to humans are needed.
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•The nationwide occurrence of PFAAs in drinking water of China was investigated.•PFBA, PFOA, and PFHxS were dominated components in drinking water.•PFAAs concentration in drinking water varied among different regions and city levels of China.•PFAAs in drinking water might not pose a serious risk in most sample sites.
Assessing the health risk of PAHs in sediments was quite difficult because sediment occurred in sea floor, and it was very hard to contact with them directly for humans. This study was attempted to ...reveal the relationship between concentrations of PAHs in surface sediments and health risk of seafood consumers. The transfer (bioaccumulation) of PAHs from surface sediment into benthic organisms was predicted. Source contributions to PAHs and related toxicity and health risks (from intake of PAHs-contaminated benthic organisms) were studied based on PMF model and Monte Carlo simulation, respectively. Total concentrations of PAHs (TPAHs) ranged from 149.40 to 1211.97ngg−1 in sediments of Bohai Sea (BS), China. Petroleum and vehicular emission, coal combustion and coke oven constituted 40.0%, 32.2% and 27.8% of PAHs, respectively, but contributed 53.0%, 22.8% and 24.2% of toxicity posed by PAHs in sediment. For children, teens and adults, the 95th percentile carcinogenic and non-carcinogenic risks were below the threshold values of 10−6 and 1.0, respectively, suggesting no potential health risk. Sensitivity analysis suggested that exposure duration (ED) and PAH concentrations (CS) were the two most sensitive parameters in risk assessment. The results provided a method to evaluate the quality of sediments and the potential health risk related to PAHs in marine sediments.
•Transfer of PAHs from sediment to mollusk, shrimp and crab in BS was predicted.•Petroleum and emission are the key contributing to both PAHs and the related TEQ.•95th percentile CR and non-CR values suggested no potential health risk in BS.
•Coal combustion, vehicle emission and coke oven are key PAH sources in BS and NPYS.•Combined biological toxicity of PAHs is lower than that of the individual PAHs.•TEQcarcs suggest potentially low ...carcinogenicity of PAHs exposure in BS and NPYS.
Sources and ecological risk of sixteen priority PAHs in surface sediments from Bohai Sea (BS) and northern part of the Yellow Sea (NPYS) were investigated. The total concentrations of PAHs varied in ranges 149.24–1211.81 and 148.28–907.47ng/g for BS and NPYS, respectively. Principal component analysis-multiple linear regression (PCA–MLR) suggested that coal combustion, vehicular emission and coke oven could be the primary PAH contributors, accounting for 56.6%, 29.2% and 14.2% of PAH concentrations, respectively. Analysis with sediment quality guidelines (SQGs) indicated that acenaphthylene, fluorene, phenanthrene, fluoranthene, pyrene and dibenzo(a,h)anthracene may occasionally cause adverse biological effects in some stations. Organic carbon (OC)-normalized analysis and mean effects range-median quotient (M-ERM-Q) suggested that the combined ecological risk of PAHs was generally low. The toxic equivalent concentrations of carcinogenic PAHs were 12.87–64.6ng/g-BaPeq and 5.95–68.80ng/g-BaPeq in BS and NPYS, respectively, suggesting low carcinogenic risk for both BS and NPYS.
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