•Traces of CH in the C4A3$-C2S system were characterized using XRD, TG, FTIR, and pH.•The RQPA method was used to determine the compositions of the C4A3$-C2S hydration.•The hydration process and ...evolution of the C4A3$-C2S system were investigated.
A C4A3$-C2S (ye'elimite-belite) clinker system with different C2S and C4A3$ contents is synthesized from analytical reagents and a C4A3$-C2S hydration system is established with a water-cement ratio of 0.4. The traces of calcium hydroxide or portlandite (CH) in the C4A3$-C2S hydration system were characterized using X-ray diffraction (XRD), thermo-gravimetric (TG) analysis, Fourier transform infrared (FTIR) spectroscopy and pH tests. The Rietveld quantitative phase analysis (RQPA) method was used to determine the compositions of the C4A3$-C2S hydration products. The hydration process and microstructure evolution of the system were also investigated. The hydration of the C4A3$-C2S involves two key process: one the consumption of AH3 phase and another the generation and stabilization of CH phase. The occurrence of the CH traces in C4A3$-C2S hydration system can be described in terms of the changes in the C2S content and the curing age. When the C2S content is less than 55 wt%, the newly generated AH3 preferentially reacts with the C2S to generate C2ASH8. When the content of C2S is between 55 wt% and 85 wt%, the C2S begins direct hydration but the generated CH is immediately consumed by the C2ASH8. When the C2S content is higher than 85 wt%, the CH generated by the hydration of C2S becomes stable in the C4A3$-C2S hydration system.
6-Gingerol, the main bioactive compound of ginger, has antioxidant, anti-inflammatory, anti-cancer and neuroprotective effects. However, it is unclear whether 6-Gingerol has protective effects ...against hepatic ischemia/reperfusion (I/R) injury. In this study, the mouse liver I/R injury model and the mouse AML12 cell hypoxia/reoxygenation (H/R) model were established by pretreatment with 6-Gingerol at different concentrations to explore the potential effects of 6-Gingerol. Serum transaminase levels, liver necrotic area, cell viability, inflammatory response, and cell apoptosis were used to assess the effect of 6-Gingerol on hepatic I/R or cell H/R injury. Quantitative polymerase chain reaction (qPCR) and Western blotting were used to detect the mRNA and protein expression. The results show that 6-Gingerol decreased serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) levels, liver necrosis, inflammatory cytokines IL-1β, IL-6, MCP-1, TNF-α expression, Ly6g+ inflammatory cell infiltration, protein phosphorylation of NF-κB signaling pathway, Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) positive cells, cell apoptosis rate, the protein expression of pro-apoptotic protein BAX and C-Caspase3, increased cell viability, and expression of anti-apoptotic protein BCL-2. Moreover, 6-Gingerol could increase the mRNA and protein expression of mitogen activated protein kinase phosphatase 5 (MKP5) and inhibit the activation of P38/JNK signaling pathway. In MKP5 knockout (KO) mice, the protective effect of 6-gingerol and the inhibition of P38/JNK pathway were significantly weakened. Therefore, our results suggest that 6-Gingerol exerts anti-inflammatory and anti-apoptotic effects to attenuate hepatic I/R injury by regulating the MKP5-mediated P38/JNK signaling pathway.
Mitogen activated protein kinase phosphatase 5 (MKP5) is a member of the MKP family and has been implicated in diverse biological and pathological conditions. However, it is unknown what role MKP5 ...plays in liver ischemia/reperfusion (I/R) injury. In the present study, we used MKP5 global knockout (KO) and MKP5 overexpressing mice to establish a liver I/R injury model in vivo, and MKP5 knockdown or MKP5 overexpressing HepG2 cells to establish a hypoxia-reoxygenation (H/R) model in vitro. In this study we demonstrated that protein expression of MKP5 was significantly downregulated in liver tissue of mice after I/R injury, and HepG2 cells subjected to H/R injury. MKP5 KO or knockdown significantly increased liver injury, as demonstrated by elevated serum transaminases, hepatocyte necrosis, infiltrating inflammatory cells, secretion of pro-inflammatory cytokines, apoptosis, oxidative stress. Conversely, MKP5 overexpression significantly attenuated liver and cell injury. Furthermore, we showed that MKP5 exerted its protective effect by inhibiting c-Jun N-terminal kinase (JNK)/p38 activity, and its action was dependent on Transforming growth factor-β-activated kinase 1 (TAK1) activity. According to our results, MKP5 inhibited the TAK1/JNK/p38 pathway to protect liver from I/R injury. Our study identifies a novel target for the diagnosis and treatment of liver I/R injury.
Hepatic ischemia-reperfusion (I/R) injury, a common clinical complication of liver transplantation, gravely affects patient prognosis. Krüppel-like factors (KLFs) constitute a family of C2/H2 zinc ...finger DNA-binding proteins. KLF6, a member of the KLF protein family, plays crucial roles in proliferation, metabolism, inflammation, and injury responses; however, its role in HIR is largely remains unknown. After I/R injury, we found that KLF6 expression in mice and hepatocytes was significantly upregulated. Mice were then subjected to I/R following injection of shKLF6- and KLF6-overexpressing adenovirus through the tail vein. KLF6 deficiency markedly exacerbated liver damage, cell apoptosis, and activation of hepatic inflammatory responses, whereas hepatic overexpression of KLF6 in mice produced the opposite results. In addition, we knocked out or overexpressed KLF6 in AML12 cells before exposing them to a hypoxia-reoxygenation challenge. KLF6 knockout decreased cell viability and increased hepatocyte inflammation, apoptosis, and ROS, whereas KLF6 overexpression had the opposite effects. Mechanistically, KLF6 inhibited the overactivation of autophagy at the initial stage, and the regulatory effect of KLF6 on I/R injury was autophagy-dependent. CHIP-qPCR and luciferase reporter gene assays confirmed that KLF6 bound to the promoter region of Beclin1 and inhibited its transcription. Additionally, KLF6 activated the mTOR/ULK1 pathway. Finally, we performed a retrospective analysis of the clinical data of liver transplantation patients and identified significant associations between KLF6 expression and liver function following liver transplantation. In conclusion, KLF6 inhibited the overactivation of autophagy via transcriptional regulation of Beclin1 and activation of the mTOR/ULK1 pathway, thereby protecting the liver from I/R injury. KLF6 is expected to serve as a biomarker for estimating the severity of I/R injury following liver transplantation.
With the development of computer technology and the arrival of the era of artificial intelligence, the analysis of user demand bias is of great significance to the operation optimization of ...e-commerce platforms. Combined with CS domain signaling data, IP packet data of PS domain, and customer CRM data provided by operators, this research studies each dimension index of operator user portrait, after that the operator user portrait platform is divided into some individual subunits, and then the corresponding data mining technology is carried out to study the implementation scheme of each subunit. The system can process and mine multidimensional data of operators’ users and form user portraits on the basis of user data aggregation. Finally, based on the operator user portrait platform studied in this paper, the operator user data are analyzed from both the user’s mobile phone use behavior and user consumption behavior. Furthermore, the application value of this research in the precision marketing and personalized service of operators is illustrated.
In order to solve the problems of low security and response efficiency and slow running speed of the current designed higher education system, a higher education system based on artificial ...intelligence technology is designed. Firstly, according to the characteristics of artificial intelligence technology, intelligent teaching system, agent technology, and data mining technology are introduced in detail. Then it analyzes the overall and detailed functional requirements of the system and adaptively generates knowledge content and teaching mode suitable for students’ ability and personality by using intelligent reasoning ability and the collection and analysis of students’ personality characteristics. Through data mining of intelligent teaching system, the decision tree about curriculum is obtained, and the students’ cognitive ability is calculated. Based on the theory of cognitive science, using the “double master” teaching mode, combined with agent technology and intelligent teaching system, the system function is divided into six modules. Through the design of database structure and data table, the design of higher education system based on artificial intelligence technology is realized. The experimental results show that the proposed method has high security and response efficiency, fast running speed, and good teaching effect.
The electroreduction of carbon dioxide (CO2) to multi‐carbon (C2+) compounds offers a viable approach for the up‐conversion of greenhouse gases into valuable fuels and feedstocks. Nevertheless, ...current industrial applications face limitations due to unsatisfactory conversion efficiency and high overpotential. Herein, a facile and scalable plasma fluorination method is reported. Concurrently, self‐evolution during CO2 electroreduction is employed to control the active sites of Cu catalysts. The copper catalyst modified with fluorine exhibits an impressive C2+ Faradaic efficiency (FE) of 81.8% at a low potential of −0.56 V (vs a reversible hydrogen electrode) in an alkaline flow cell. The presence of modified fluorine leads to the exposure and stabilization of high‐activity Cu+ species, enhancing the adsorption of *CO intermediates and the generation of *CHO, facilitating the subsequent dimerization. This results in a notably improved conversion efficiency of 13.1% and a significant reduction in the overpotential (≈100 mV) for the C2+ products. Furthermore, a superior C2+ FE of 81.6% at 250 mA cm−2, coupled with an energy efficiency of 31.0%, can be achieved in a two‐electrode membrane electrode assembly electrolyzer utilizing the fluorine‐modified copper catalyst. The strategy provides novel insights into the controllable electronic modification and surface reconstruction of electrocatalysts with practical potential.
A facile and scalable plasma fluorination method is proposed to manufacture integrated gas diffusion electrodes of fluorine‐modified copper catalysts, which can precisely regulate the amount and depth of fluorine implantation on the copper to exhibit an impressive multi‐carbon products Faradaic efficiency of 81.8% at a low potential of −0.56 V in an alkaline flow cell.
The correct individual tree segmentation of the forest is necessary for extracting the additional information of trees, such as tree height, crown width, and other tree parameters. With the ...development of LiDAR technology, the research method of individual tree segmentation based on point cloud data has become a focus of the research community. In this work, the research area is located in an underground coal mine in Shenmu City, Shaanxi Province, China. Vegetation information with and without leaves in this coal mining area are obtained with airborne LiDAR to conduct the research. In this study, we propose hybrid clustering technique by combining DBSCAN and K-means for segmenting individual trees based on airborne LiDAR point cloud data. First, the point cloud data are processed for denoising and filtering. Then, the pre-processed data are projected to the XOY plane for DBSCAN clustering. The number and coordinates of clustering centers are obtained, which are used as an input for K-means clustering algorithm. Finally, the results of individual tree segmentation of the forest in the mining area are obtained. The simulation results and analysis show that the new method proposed in this paper outperforms other methods in forest segmentation in mining area. This provides effective technical support and data reference for the study of forest in mining areas.
Bound states in the continuum (BICs) have a superior ability to confine electromagnetic waves and enhance light–matter interactions. However, the quality‐factor of quasi‐BIC is extremely sensitive to ...structural perturbations, thus the BIC metasurfaces usually require a very‐high precision nanofabrication technique that greatly restricts their practical applications. Here, distinctive 2.5D out‐of‐plane architectures based plasmonic symmetry protected (SP)‐BIC metasurfaces are proposed, which could deliver robust quality factors even with large structural perturbations. The high‐throughput fabrication of such SP‐BIC metasurfaces is realized by using the binary‐pore anodic aluminum oxide template technique. Moreover, the deep neural network (DNN) is adapted to conduct multiparameter fittings, where the 2.5D hetero‐out‐of‐plane architectures with robust high quality‐factors and figures of merit are rapidly predicted and fabricated. Finally, owning to its large second‐order surface sensitivity, the desired 2.5D hetero‐out‐of‐plane architecture demonstrates a detection limit of endotoxin as low as 0.01 EU mL‐1, showing a good perspective of biosensors and others.
A series of 2.5D out‐of‐plane architectures are proposed by using a combination of binary‐pore anodic aluminum oxide template technique and deep neural network to efficiently realize plasmonic symmetry protected bound states in the continuum (BIC) metasurfaces with robust high quality‐factors and figures of merits that show a good perspective of biosensors and others.
Acute pancreatitis (AP) is a common emergency of the digestive system and serious cases can develop into severe acute pancreatitis (SAP), which ortality rates up to 30%. Sirtuin4 (SIRT4) is a member ...of the sirtuin family, and plays a key role in inflammation and oxidative stress. However, the potential role of SIRT4 in SAP has yet to be elucidated. In the present study, we found that the expression level of SIRT4 in human AP was downregulated by screening a public database, suggesting that SIRT4 may play a role in AP. Subsequently, we used L-arginine (L-Arg) to induce SAP in SIRT4 knockout (SIRT4_KO) and SIRT4 overexpression (AAV_SIRT4) mice. The results showed that the pancreatic tissue injury and related lung and kidney injury were serious in SIRT4_KO mice after SAP induction, but were significantly reduced in AAV_SIRT4 mice. More importantly, we found that the levels of antioxidant factors GSH and SOD were decreased in SIRT4_KO mice, and the production of oxidative products and lipid peroxidation markers was increased, suggesting that SIRT4 was involved in inflammation and oxidative stress during SAP. Further studies showed that the absence or overexpression of SIRT4 affected the expression level of Hypoxia-inducible factor-1α (HIF-1α) after SAP induction, and regulated the expression of ferroptosis related proteins by mediating HIF-1α/HO-1 pathway. Collectively, our study revealed that SIRT4 plays a protective role in SAP by regulating the HIF-1α/HO-1 pathway to inhibit ferroptosis.
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