In the process of asphalt pavement maintenance management, the technical condition of the pavement is accurately predicted, its performance trend is predicted, and the right time is chosen to take ...targeted maintenance measures that will play a multiplier role, so predicting the technical condition of the pavement is important significance. This paper mainly studies the cluster dimensionality reduction evaluation method of the technical condition of expressway asphalt pavement. This paper summarizes the experience of regular highway inspection data inspection and analysis, and mainly studies the standardized analysis method of highway pavement inspection data, aiming to meet the data requirements at all levels through effective data analysis indicators. The experimental data in this paper show that the driving quality of the two-way pavement is good, the total average SRI is 91.2, and the evaluation is “excellent,” and the left width is slightly better than the right width; the transverse crack penetration of most reconstructed road sections is greater than 0.4, indicating that the transverse crack penetrates the road surface more serious. The experimental results of this paper show that the technical status of asphalt pavement based on cluster analysis has practical significance, and the analysis results show that the treatment effect is ideal.
Redox homeostasis, as an innate cellular defense mechanism, not only contributes to malignant transformation and metastasis of tumors, but also seriously restricts reactive oxygen species ...(ROS)-mediated tumor therapies, such as chemotherapy, radiotherapy, photodynamic therapy (PDT), and chemodynamic therapy (CDT). Therefore, the development of the redox dyshomeostasis (RDH) strategy based on nanomaterials chemistry is of great significance for developing highly efficient tumor therapy. This review will firstly introduce the basic definition and function of cellular redox homeostasis and RDH. Subsequently, the current representative progress of the nanomaterial-based RDH strategy for tumor therapy is evaluated, summarized and discussed. This strategy can be categorized into three groups: (1) regulation of oxidizing species; (2) regulation of reducing species and (3) regulation of both of them. Furthermore, the current limitations and potential future directions for this field will be briefly discussed. We expect that this review could attract positive attention in the chemistry, materials science, and biomedicine fields and further promote their interdisciplinary integration.
This review summarizes the current progress of the redox dyshomeostasis (RDH) strategy for tumor therapy. This strategy makes tumor cells more sensitive to current therapy patterns through using nanomaterials to disrupt redox homeostasis.
MYB transcription factors (TFs), as one of the largest gene families in plants, play important roles in multiple biological processes, such as plant growth and development, cell morphology and ...pattern building, physiological activity metabolism, primary and secondary metabolic reactions, and responses to environmental stresses. The function of MYB TFs in crops has been widely studied, but few studies have been done on medicinal plants. In this review, we summarized the MYB TFs that play important roles in secondary metabolism and emphasized the possible mechanisms underlying how MYB TFs are regulated at the protein, posttranscriptional, and transcriptional levels, as well as how they regulate the downstream target gene networks related to secondary metabolism in plants, especially in medicinal plants.
Using two datasets from the Canadian Perspectives Survey Series (CPSS), this study provides a longitudinal analysis of information sources Canadians consulted regarding COVID-19 and their ...associations with poor self-perceived mental health (SPMH) during March and July 2020. Nearly 20% of Canadians who were surveyed reported poor SPMH. The logistic regression results revealed that at Time 2 (July 2020), after controlling for demographic, socio-economic, and psychobehavioural factors, using social media was significantly associated with higher odds of poor SPMH than using six other information sources including news outlets, federal health agencies, provincial health agencies, provincial daily announcements, places of employment, and other sources (for example, schools, colleges, universities). Checking the accuracy of online information more frequently was also associated with lower odds of poor SPMH.
Severe acute respiratory syndrome-coronavirus (SARS-CoV) and SARS-like coronavirus are a potential threat to global health. However, reviews of the long-term effects of clinical treatments in SARS ...patients are lacking. Here a total of 25 recovered SARS patients were recruited 12 years after infection. Clinical questionnaire responses and examination findings indicated that the patients had experienced various diseases, including lung susceptibility to infections, tumors, cardiovascular disorders, and abnormal glucose metabolism. As compared to healthy controls, metabolomic analyses identified significant differences in the serum metabolomes of SARS survivors. The most significant metabolic disruptions were the comprehensive increase of phosphatidylinositol and lysophospha tidylinositol levels in recovered SARS patients, which coincided with the effect of methylprednisolone administration investigated further in the steroid treated non-SARS patients with severe pneumonia. These results suggested that high-dose pulses of methylprednisolone might cause long-term systemic damage associated with serum metabolic alterations. The present study provided information for an improved understanding of coronavirus-associated pathologies, which might permit further optimization of clinical treatments.
Long non-coding RNA (lncRNA) plays important roles in many diseases, including pulmonary hypertension. The anti-diabetic drug metformin has been discovered to have protective effect on experimental ...pulmonary artery hypertension (PAH). However, the exact mechanism of metformin on the expression of lncRNA in PAH remains unclear. Here, we applied microarray analysis to examine lncRNA and mRNA expression proflies in pulmonary arteries (PAs) tissues of PAH rats with or without metformin treatment. A total of 24 lncRNAs (14 upregulated and 10 down-regulated) and 82 mRNAs (17 upregulated and 65 down-regulated) were differently expressed in PAH rats induced by MCT, whereas 83 lncRNAs (59 upregulated and 24 down-regulated) and 145 mRNAs (110 upregulated and 35 down-regulated) were differently expressed after metformin treatment. The expression levels of lncRNAs (NONRATT015587.2, NONRATT006975.2, NONRATT031226.2 and NONRATT024291.2 et al) were verified through realtime-PCR, and the results of NONRATT015587.2 and NONRATT024291.2 were consistent with RNA sequencing. Bioinformatics analysis was performed, including gene ontology (GO) analysis and genomes (KEGG) analysis. Transcription factor (TF)-target network analysis revealed that metformin regulated gene expression potentially via TFs including Tp53, Est1, Sp1 and Hif1α. The analysis illustrated that overexpression of NONRATT015587.2 promoted proliferation and knockdown of NONRATT015587.2 increased apoptosis of pulmonary artery smooth muscle cells in vitro, both of which were implicated in vascular remodeling in PAH. In addition, we found that the p53 and Hif1α signaling pathways may be involved in this regulation process. NONRATT015587.2 can be expected as a novel candidate in diagnostic markers for PAH. Our results provide novel insight into the mechanisms of the pivotal lncRNA-mRNA interactions, and indicate that NONRATT015587.2 acts as a pro-proliferative factor in regulation of pulmonary vascular remodeling.
Vanadium dioxide (VO2) with semiconductor-metal phase transition characteristics has presented great application potential in various optoelectrical smart devices. However, the preparation of doped ...VO2 film with a lower phase transition threshold on Si substrate needs more investigation for the exploration of silicon-based VO2 devices. In this work, the VO2 films doped with different contents of W element were fabricated on high-purity Si substrate, assisted with a post-annealing process. The films exhibited good crystallinity and uniform thickness. The X-ray diffraction and X-ray photoelectron spectroscopy characterizations illustrated that W element can be doped into the lattice of VO2 and lead to small lattice distortion. In turn, the in situ FT-IR measurements indicated that the phase transition temperature of the VO2 films can be decreased continuously with W doping content. Simultaneously, the doping would lead to largely enhanced conductivity in the film, which results in reduced optical transmittance. This work provides significant insights into the design of doped VO2 films for silicon-based devices.
The phytohormones abscisic acid (ABA) and indole-3-acetic acid (IAA) response pathways interact synergistically or antagonistically to regulate plant development and environmental adaptation. Here, ...we show that ABI1, a key negative regulator of ABA signaling, is essential for auxin-modulated root development. We performed a microarray analysis using the loss-of-function mutant abi1-3 and Col-0 seedlings treated with IAA. For sHSP22, an endoplasmic reticulum (ER) small heat shock protein-encoding gene, the induction by IAA was dependent on ABI1. shsp22 displayed enhanced sensitivity to ABA in primary root growth. In contrast, overexpression of full-length, but not truncated sHSP22 lacking signal peptide and ER-retention sequences, resulted in decreased ABA sensitivity. Overexpressed (OX) sHSP22 partially rescued the ABA hypersensitivity of abi1-3. In addition, sHSP22 is involved in auxin-regulated hypocotyl elongation at high temperature treatment. sHSP22 also affected accumulation of auxin efflux carrier PIN proteins due to potentiated intracellular trafficking. And sHSP22 OX lines initiated more lateral roots after auxin application. Our results suggest that sHSP22 regulates auxin response through modulating auxin polar transport, and ABI1-sHSP22 provides a novel module orchestrating ABA and auxin signaling crosstalk in Arabidopsis (Arabidopsis thaliana).
Different conformations, including planar, corrugated, as well as the deficient structure of the two-dimensional materials, play a relevant role in determining their catalytic reaction performances. ...Here, we systematically investigated the stabilities, electronic properties, and nitrogen activities capacity of various vacancy-modified g-C3N4 considering two different conformations (planar and corrugated) to explore the effects of nitrogen vacancy (NV) and conformations on the photocatalytic performance of g-C3N4 by means of density functional theory computations. Our results found that not only can the nitrogen vacancy (NV) promote separation efficiency of the photoinduced carriers in g-C3N4 but also the distortion conformation can activate more n → π* transitions of NV g-C3N4, resulting in a red shift of optical absorption spectra. More importantly, our results reveal that the corrugation configuration structure, compared to planar conformation, is much more favorable to photocatalytic nitrogen fixation reaction from the aspects of nitrogen absorption capacity and free-energy change, in which corrugation model with N2C vacancy has the smallest onset potential (1.32 V) for the most difficult step through the alternating pathway.
The present study delineates the interaction of a typical PRRSV1.1 isolate 3267 (moderate virulence) with
in vitro
derived pig conventional dendritic cells, cDC1, cDC2, and a CD14
+
population ...(designated as CD14
+
DCs). cDC1 and cDC2 were not susceptible to 3267 infection, but a fraction of CD14
+
DCs were infected. After exposure to the virus, all three DC types remained immature as determined by no increase of maturation molecules (MHC-I, MHC-II, CD80/86, CCR7), no release of cytokines, no modification of antigen presentation abilities, and no alteration of endocytic/phagocytic capabilities. However, when infected MARC-145 cells were used as a source of viral antigens, cDC2 and CD14
+
DCs showed a significant increase in the expression of maturation molecules and substantial release of cytokines, notably IL-12/IL-23p40 (by both DC types) and IL-10 (by CD14
+
DCs). To address the impact of PRRSV1 3267 on TLR3- and TLR7-mediated activation, cDC1, cDC2, and CD14
+
DCs were inoculated by the virus (live or UV-inactivated) for 6 h prior to or simultaneously with the addition of poly I:C (TLR3 ligand) or gardiquimod (TLR7 ligand; not used for cDC1). Compared with using TLR ligand alone, combination with the virus did not result in any alteration to the maturation markers on all DC types but changed the cytokine response to either TLR3 or TLR7 ligand. Pre-exposure of cDC2 or CD14
+
DCs to the live virus resulted in an increased production of IFN-α upon poly I:C stimulation, while pre-exposure to UV-inactivated virus tended to enhance the release of IL-10 upon gardiquimod stimulation. Simultaneous addition of the live virus and the TLR ligand either had no effect (mainly in cDC2) or impaired most of the cytokine release after gardiquimod stimulation (in CD14
+
DCs). When used as antigen presenting cells, cDC2 pre-inoculated by the live virus before addition of gardiquimod impaired the proliferation of CD4
–
CD8
–
T cells. In the case of CD14
+
DCs, pre-exposure to the live virus or simultaneously added with TLR3 or TLR7 ligand largely decreased the proliferation of CD4
–
CD8
+
and CD4
–
CD8
+
T-cell subsets. For cDC1, no significant changes were observed in cytokine responses or T-cell proliferation after poly I:C stimulation. Of note, cDC1 had a short life during
in vitro
culturing, for which the results obtained might be biased. Overall, exposure to PRRSV1 did not induce maturation of cDC1, cDC2, or CD14
+
DCs, but modified TLR3 and TLR7-associated responses (except for cDC1), which may affect the development of adaptive immunity during PRRSV1 infection. Moreover, the sensing of infected cells was different from that of the free virus.
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