Increasing evidence has underscored the role of long non-coding RNAs (lncRNAs) acting as competing endogenous RNAs (ceRNAs) in the development and progression of tumors. Nevertheless, lncRNA ...biomarkers in lncRNA-related ceRNA network that can predict the prognosis of breast cancer (BC) are still lacking. The aim of our study was to identify potential lncRNA signatures capable of predicting overall survival (OS) of BC patients.
The RNA sequencing data and clinical characteristics of BC patients were obtained from the Cancer Genome Atlas database, and differentially expressed lncRNA (DElncRNAs), DEmRNAs, and DEmiRNAs were then identified between BC and normal breast tissue samples. Subsequently, the lncRNA-miRNA-mRNA ceRNA network of BC was established, and the gene oncology enrichment analyses for the DEmRNAs interacting with lncRNAs in the ceRNA network was implemented. Using univariate and multivariate Cox regression analyses, a four-lncRNA signature was developed and used for predicting the survival in BC patients. We applied receiver operating characteristic analysis to assess the performance of our model.
A total of 1061 DElncRNAs, 2150 DEmRNAs, and 82 DEmiRNAs were identified between BC and normal breast tissue samples. A lncRNA-miRNA-mRNA ceRNA network of BC was established, which comprised of 8 DEmiRNAs, 48 DElncRNAs, and 10 DEmRNAs. Further gene oncology enrichment analyses revealed that the DEmRNAs interacting with lncRNAs in the ceRNA network participated in cell leading edge, protease binding, alpha-catenin binding, gamma-catenin binding, and adenylate cyclase binding. A univariate regression analysis of the DElncRNAs revealed 7 lncRNAs (ADAMTS9-AS1, AC061992.1, LINC00536, HOTAIR, AL391421.1, TLR8-AS1 and LINC00491) that were associated with OS of BC patients. A multivariate Cox regression analysis demonstrated that 4 of those lncRNAs (ADAMTS9-AS1, LINC00536, AL391421.1 and LINC00491) had significant prognostic value, and their cumulative risk score indicated that this 4-lncRNA signature independently predicted OS in BC patients. Furthermore, the area under the curve of the 4-lncRNA signature associated with 3-year survival was 0.696.
The current study provides novel insights into the lncRNA-related ceRNA network in BC and the 4 lncRNA biomarkers may be independent prognostic signatures in predicting the survival of BC patients.
Currently, traditional predictors of prognosis (tumor size, nodal status, progesterone receptor PR, estrogen receptor ER, or human epidermal growth factor receptor‐2 HER2) are insufficient for ...precise survival prediction for triple‐negative breast cancer (TNBC). Long noncoding RNAs (lncRNAs) have been observed to exert critical functions in cancer, including in TNBC. Nevertheless, systematically tracking expression‐based lncRNA biomarkers based on the sequence data for the prediction of prognosis in TNBC has not yet been investigated. To ascertain whether biomarkers exist that can distinguish TNBC from adjacent normal tissue or nTNBC, we implemented a comprehensive analysis of lncRNA expression profiles and clinical data of 1097 BC samples from The Cancer Genome Atlas database. A total of 1510 differentially expressed lncRNAs in normal and TNBC samples were extracted. Similarly, 672 differentially expressed lncRNAs between nTNBC and TNBC samples were detected. The receiver operating characteristic curve analysis indicated that three upregulated lncRNAs (AC091043.1, AP000924.1, and FOXCUT) may be of strong diagnostic value for predicting the existence of TNBC in the training and validation sets (area under the curve (AUC > 0.85). Kaplan‐Meier analysis demonstrated that the other three lncRNAs (AC010343.3, AL354793.1, and FGF10‐AS1) were associated with the prognosis of TNBC patients (P < 0.05). We used the three overall survival (OS)‐related lncRNAs to establish a three‐lncRNA signature. Multivariate Cox regression analysis suggested that the three‐lncRNA signature was a prognostic factor independent of other clinical variables (
P < 0.01) for predicting OS in TNBC patients that could be utilized to classify patients into high‐ or low‐risk subgroups. Our results might provide efficient signatures for clinical diagnosis and prognostic evaluation of TNBC.
1.
Receiver operating characteristic (ROC) curve showed that three upregulated long noncoding RNAs (lncRNAs) (AC091043.1, AP000924.1, and FOXCUT) may be of strong diagnostic value to predict the existence of triple‐negative breast cancer (TNBC) in the training and validation sets (area under the curve AUC > 0.85.
2.
Kaplan‐Meier analysis was carried out to detect other three‐lncRNAs (AC010343.3, AL354793.1, and FGF10‐AS1) associated with patients’ prognosis (P < 0.05).
3.
Multivariate Cox regression analysis suggested that the three‐lncRNA signature was an independent prognostic factor of other clinical variables (P < 0.01) for predicting overall survival (OS) in TNBC patients that was able to classify patients into high‐ and low‐risk subgroups.
Abstract
Kinematic signatures of the jet, winds, multicavities, and episodic shells arising in the unified model of bipolar outflows developed in Shang et al. (Paper I), in which an outflow forms by ...radially directed, wide-angle toroidally magnetized winds interacting with magnetized isothermal toroids, are extracted in the form of position–velocity diagrams. Elongated outflow lobes, driven by magnetized winds and their interplay with the environment, are dominated by extended bubble structures with mixing layers beyond the conventional thin-shell models. The axial cylindrically stratified density jet carries a broad profile near the base, across the projected velocity of the wide-angle wind, and narrows down along the axis with the collimated flow. The reverse shock encloses the magnetized free wind, forms an innermost cavity, and deflects the flow pattern. Shear, Kelvin–Helmholtz instabilities, and pseudopulses add fine and distinctive features between the jet–shell components, and the fluctuating jet velocities. The broad webbed velocity features connect the extremely high and the low velocities across the multicavities, mimicking nested outflowing slower-wind components. Rings and ovals in the perpendicular cuts trace multicavities at different heights, and the compressed ambient gap regions enrich the low-velocity features with protruding spikes. Our kinematic signatures capture the observed systematics of the high-, intermediate-, and low-velocity components from Class 0 to II jet–outflow systems in molecular and atomic lines. The nested shells observed in HH 212, HH 30, and DG Tau B are naturally explained. Outflows as bubbles are ubiquitous and form an inevitable integrative outcome of the interaction between wind and ambient media.
The real-time electricity consumption data can be used in value-added service such as big data analysis, meanwhile the single user's privacy needs to be protected. How to balance the data utility and ...the privacy preservation is a vital issue, where the privacy-preserving data aggregation could be a feasible solution. Most of the existing data aggregation schemes rely on a trusted third party (TTP). However, this assumption will have negative impact on reliability, because the system can be easily knocked down by the denial of service attack. In this paper, a practical privacy-preserving data aggregation scheme is proposed without TTP, in which the users with some extent trust construct a virtual aggregation area to mask the single user's data, and meanwhile, the aggregation result almost has no effect for the data utility in large scale applications. The computation cost and communication overhead are reduced in order to promote the practicability. Moreover, the security analysis and the performance evaluation show that the proposed scheme is robust and efficient.
Abstract The young stellar source HH 30 is a textbook example of an ionic optical jet originating from a disk in an edge-on system shown by the Hubble Space Telescope. It has a remnant envelope in 12 ...CO observed by the Atacama Large Millimeter/submillimeter Array. The optical jet is characterized by its narrow appearance, large line width at the base, and high temperature inferred from line diagnostics. Three featured structures can be identified, most evident in the transverse position–velocity diagrams: an extremely-high-velocity wide-angle wind component with large spectral widths in the optical, a very-low-velocity ambient surrounding medium seen in 12 CO, and a low-velocity region traced by 12 CO nested both in velocity and location between the primary wind and ambient environment. A layered cavity with multiple shells forms nested morphological and kinematic structures around the optical jet. The atomic gas originating from the innermost region of the disk attains a sufficient temperature and ionization to emit brightly in forbidden lines as an optical jet. The wide-angle portion expands, forming a low-density cavity. The filamentary 12 CO encompassing the wind cavity is mixed and advected inward through the action of the magnetic interplay of the wide-angle wind with the molecular ambient medium. The magnetic interplay results in the layered shells penetrating deeply into the vast cavity of tenuous atomic wind material. The HH 30 system is an ideal manifestation of the unified wind model of Shang et al. (2020, 2023), with clearly distinguishable atomic and molecular species mixed through the atomic lightly ionized magnetized wind and the surrounding cold molecular ambient material.
Abstract
We explore a new, efficient mechanism that can power toroidally magnetized jets up to two to three times their original terminal velocity after they enter a self-similar phase of magnetic ...acceleration. Underneath the elongated outflow lobe formed by a magnetized bubble, a wide-angle free wind, through the interplay with its ambient toroid, is compressed and accelerated around its axial jet. The extremely magnetic bubble can inflate over its original size, depending on the initial Alfvén Mach number
M
A
of the launched flow. The shape-independent slope ∂
v
r
/∂
r
= 2/3
t
is a salient feature of the self-similarity in the acceleration phase. Peculiar kinematic signatures are observable in the PV diagrams and can combine with other morphological signatures as probes for density-collimated jets arising in toroidally dominated magnetized winds. The apparent second acceleration is powered by the decrease of the toroidal magnetic field but operates far beyond the scales of the primary magnetocentrifugal launch region and the free asymptotic terminal state. Rich implications may connect the jets arising from the youngest protostellar outflows such as HH 211 and HH 212 and similar systems with parsec-scale jets across the mass and evolutionary spectra.
MicroRNA miR-31 is implicated in the neoplastic process of various malignancies including oral squamous cell carcinoma (OSCC). Silent information regulator 3 (Sirtuin3 or SIRT3) is a NAD-dependent ...deacetylase that regulates metabolic process. Suppressor role of SIRT3 has been found in neoplasms. This study investigates the disruptions of miR-31-SIRT3 cascade to explore their potential association with metabolic change in OSCC. We identified that miR-31 directly targeted SIRT3 in OSCC cells, and a reverse correlation between miR-31 expression and SIRT3 expression was noted in OSCC tumors. SIRT3 expression attenuated the miR-31 enhanced tumor cell migration and invasion. It also reduced the tumorigenic potential of FaDu cell line. miR-31-SIRT3 impaired the mitochondrial membrane potential and structural integrity. The dis-regulation of this axis also contributed to the genesis of oxidative stress. In addition, miR-31 switched tumor cells from aerobic metabolism to glycolytic metabolism. This study provides novel evidences demonstrating the presence of miR-31-mediated post-transcriptional regulation of SIRT3 in OSCC. The disruption of miR-31-SIRT3 cascade and the consequential metabolic aberrances are involved in OSCC progression.
•miR-31 targets SIRT3 to drive OSCC invasion.•OSCC tumors exhibit miR-31 upregulation and SIRT3 downregulation.•miR-31-SIRT3 disruption underlies the mitochondrial disturbance.•miR-31-SIRT3 cascade induces ROS, glycolytic metabolism and lactate production.
Direct detection of circularly polarized light (CPL) is a challenging task due to limited materials and ambiguous structure–property relationships that lead to low distinguishability of the light ...helicities. Perovskite ferroelectric semiconductors incorporating chirality provide new opportunities in dealing with this issue. Herein, a pair of 2D chiral perovskite ferroelectrics is reported, which have enhanced CPL detection performance due to interplays among lattice, photon, charge, spin, and orbit. The chirality‐transfer‐induced chiral&polar ferroelectric phase enhances the asymmetric nature of the photoactive sublattice and achieves a switchable self‐powered detection via the bulk photovoltaic effect. The single‐crystal‐based device exhibits a CPL‐sensitive detection performance under 430 nm with an asymmetric factor of 0.20 for left‐ and right‐CPL differentiation, about two times that of the pure chiral counterparts. The enhanced CPL detection performance is ascribed to the Rashba–Dresselhaus effect that originates from the bulk inversion asymmetry and strong spin–orbit coupling, shown with a large Rashba coefficient, which is demonstrated by density functional theory calculation and circularly polarized light excited photoluminescence measurement. These results provide new perspectives on chiral Rashba ferroelectric semiconductors for direct CPL detection and ferroelectrics‐based chiroptics and spintronics.
A pair of 2D chiral perovskite Rashba ferroelectric semiconductors is reported. The fabricated single‐crystal device responds to circularly polarized light (CPL) under 430 nm with an anisotropy factor of 0.20 for the left‐ and right‐CPL differentiation, about two times of reported pure chiral counterparts. The enhanced performance is ascribed to the Rashba–Dresselhaus effect with a large Rashba coefficient of 0.93 eV Å.
Abstract
The presence of complex organic molecules (COMs) in the interstellar medium is of great interest since it may link to the origin and prevalence of life in the universe. Aiming to investigate ...the occurrence of COMs and their possible origins, we conducted a chemical census toward a sample of protostellar cores as part of the Atacama Large Millimeter/submillimeter Array Survey of Orion Planck Galactic Cold Clumps project. We report the detection of 11 hot corino sources, which exhibit compact emissions from warm and abundant COMs, among 56 Class 0/I protostellar cores. All of the hot corino sources discovered are likely Class 0, and their sizes of the warm region (>100 K) are comparable to 100 au. The luminosity of the hot corino sources exhibits positive correlations with the total number of methanol and the extent of its emissions. Such correlations are consistent with the thermal desorption picture for the presence of hot corinos and suggest that the lower-luminosity (Class 0) sources likely have a smaller region with COM emissions. With the same sample selection method and detection criteria being applied, the detection rates of the warm methanol in the Orion cloud (15/37) and the Perseus cloud (28/50) are statistically similar when the cloud distances and the limited sample size are considered. Observing the same set of COM transitions will bring a more informative comparison between the cloud properties.
Abstract
3D hydrogel structures fabricated by two‐photon polymerization (TPP) have become attractive in biomedical fields. Generally, conventional organic solvents are toxic and the residues left in ...the fabricated 3D structures are harmful to cells. Hence, anion ionic carbazole‐based water‐soluble two‐photon initiator (TPI) 3,6‐bis2‐(1‐methylpyridinium)vinyl‐9‐methylcarbazole ditosylate (BT) is proposed to construct arbitrary 3D hydrogel structures. Cucurbit7uril (CB7) and BT form a host‐guest complex (CB7/BT) with a binding ratio of 1:1, which further improves the water solubility, biocompatibility and nonlinear absorption property of TPI. A water‐soluble photoresist consisting of photoinitiator CB7/BT and monomer poly(ethylene glycol) diacrylate is prepared to explore the TPP fabrication capacity. The electron paramagnetic resonance measurement shows that CB7/BT initiates photopolymerization by alkyl radicals. The laser threshold power of the photoresist is 6.3 mW and the feature size is 127 nm in TPP at 780 nm. The initiator with
p
‐toluenesulfonate anion exhibits higher binding energy, larger two‐photon absorption cross‐section and two‐photon fabrication resolution compared with the previous work using iodide as an anion, indicating a promising way to improve the fabrication capacity of water‐soluble TPI through changing the anion ionic group. The proposed strategy will provide high potential for the further application in the biomedical field.
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