Licorice is a common herb which has been used in traditional Chinese medicine for centuries.More than 20 triterpenoids and nearly 300 flavonoids have been isolated from licorice. Recent studies have ...shown that these metabolites possess many pharmacological activities, such as antiviral,antimicrobial, anti-inflammatory, antitumor and other activities. This paper provides a summary of the antiviral and antimicrobial activities of licorice. The active components and the possible mechanisms for these activities are summarized in detail. This review will be helpful for the further studies of licorice for its potential therapeutic effects as an antiviral or an antimicrobial agent.
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•At least 23 OP diesters have been reported as contaminants or as degradation products of OP triesters.•OP diesters containing halogen atoms and aryl groups could be more persistent ...in environmental matrices.•Multiple sources of OP diesters existed, i.e. industrial production and biotic or abiotic degradation from OP triesters.•Total annual output of OP diesters was estimated to be 17,050 metric tons (underestimated).•Various adverse effects following exposure to OP diesters in organisms have been reported.
Over the course of the continual phase-outs of toxic halogenated flame retardants (HFRs), there has been an increasing demand for organophosphate esters (OPEs) in global FR markets. OPE-FRs have largely been identified as OP triesters, which have a basic chemical structure of O = P(OR)3. In addition to OP triesters, OPEs can refer to another class of related substances, namely, OP diesters that have a typical chemical structure of O = P(OR)2(OH)). OP diesters are known as biotic or abiotic degradation products of OP triesters. In recent years, environmental scientists have proven that OP diesters widely exist in a variety of environmental matrices and biotic samples around the world, implying the potential risks from OP diester exposure to biota and humans in the environment. Here, we have reviewed the scientific literature for studies involving OP diesters and up to the end of 2020. The aim of the present review is to assess the present understanding of the physicochemical properties, sources (industrial production and degradation), environmental occurrence of OP diesters, and adverse effects to exposed organisms. Based on the literature in the Web of Science core collection, we found that at least 23 OP diesters have been reported as contaminants in various environments or as degradation products of OP triesters. The physicochemical properties of OP diesters vary depending on their specific chemical structures. OP diesters containing halogen atoms and aryl groups seem to be more persistent (with greater estimated half-life (t1/2) values) in environmental matrices. There were multiple sources of OP diesters, including industrial production and biotic or abiotic degradation from OP triesters. Specifically, we found that ten OP diesters are produced somewhere in the world, and the total annual output was estimated to be 17,050 metric tons (this number is underestimated due to the limitation of the available information). In addition, the wide application of OP triesters worldwide makes the degradation of OP triesters another critical source of OP diesters to the environment and to organisms. Current monitoring studies have demonstrated that some OP diesters were detectable in the human body (via both blood and urine samples), indoor dust, wastewater, or sewage sludge worldwide. The highest concentrations of diphenyl phosphate (DPHP) in human urine have been reported as high as 727 ng/mL (children (aged 0–5 years) urine samples from Australia). In addition, adverse effects following direct or indirect exposure to 11 OP diesters in organisms (including animals, bacteria, and algae) have been reported, and the recorded adverse outcomes following exposure to OP diesters included developmental toxicity, alteration of gene expression, and disturbance of nuclear receptor activity. Biomonitoring studies regarding human samples have frequently reported statistically significant associations between the concentrations of OP diesters and markers of human health (mainly related to reproductive toxicity). Finally, on the basis of current knowledge on OP diesters, we propose prospects for related research directions in future studies.
•TPHP decreased growth of D. magna.•TPHP decreased fecundity of D. magna.•TPHP changed the expressions of genes involved in 15 pathways.
The additive flame retardant triphenyl phosphate (TPHP) has ...been frequently detected in environments and biota. Evidences indicate that TPHP has potential risks to aquatic organisms. Seldom has been reported about its chronic effects to aquatic organism at low trophic levels, such as Cladocera. In the present study, <12 h old Daphnia magna (D. magna) were exposed to 0, 5, 50 or 500 μg/L TPHP for 21 days to investigate the chronic effects of TPHP on body length, fecundity and survival. Meanwhile, D. magna PCR arrays were used to evaluate the transcriptional responses of 155 genes involved in 40 pathways. Exposure to 500 μg/L TPHP for 21 days significantly decreased the body lengths of both F0 and F1 generation and inhibited the fecundity of F0 generation. Results of RT-qPCR showed that the expressions of 76 genes involved in 15 pathways were significantly altered after exposure to 500 μg/L TPHP for 21 days. The significantly altered pathways related to genetic information processing, cellular process and metabolism might be responsible for the observed effects of TPHP. Overall, our results showed that chronic exposure to TPHP caused developmental and reproductive toxicities to D. magna.
Glycyrrhizin is an important bioactive compound that is used clinically to treat chronic hepatitis and is also used as a sweetener world-wide. However, the key UDP-dependent glucuronosyltransferases ...(UGATs) involved in the biosynthesis of glycyrrhizin remain unknown.
To discover unknown UGATs, we fully annotated potential UGATs from Glycyrrhiza uralensis using deep transcriptome sequencing. The catalytic functions of candidate UGATs were determined by an in vitro enzyme assay.
Systematically screening 434 potential UGATs, we unexpectedly found one unique GuUGAT that was able to catalyse the glucuronosylation of glycyrrhetinic acid to directly yield glycyrrhizin via continuous two-step glucuronosylation. Expression analysis further confirmed the key role of GuUGAT in the biosynthesis of glycyrrhizin. Site-directed mutagenesis revealed that Gln-352 may be important for the initial step of glucuronosylation, and His-22, Trp-370, Glu-375 and Gln-392 may be important residues for the second step of glucuronosylation. Notably, the ability of GuUGAT to catalyse a continuous two-step glucuronosylation reaction was determined to be unprecedented among known glycosyltransferases of bioactive plant natural products.
Our findings increase the understanding of traditional glycosyltransferases and pave the way for the complete biosynthesis of glycyrrhizin.
Growth hormone/insulin-like growth factors (GH/IGFs) axis PCR array of zebrafish (Danio rerio) larvae was developed based on the quantification of mRNA expressions of 19 genes that were confirmed to ...play vital roles in the regulation of fish growth. Zebrafish embryos were exposed to each of four concentrations of the six representative chemicals, bisphenol A (BPA), perfluorooctane sulfonates (PFOS), tris (1,3-dichloroisopropyl) phosphate (TDCIPP), cadmium chloride (CdCl2), mercury (II) chloride (HgCl2) and lead (II) acetate (PbAc) from 2 h post fertilization (hpf) to 96 hpf. Developmental endpoints and transcriptional profiles of the genes involved in GH/IGFs axis of zebrafish larvae were examined at 96 hpf. Body length of zebrfish larvae was found to be a more susceptible endpoint in zebrafish embryo toxicity test than other endpoints, including survival rate, hatching rate, malformation incidence and heart rate after exposure to each of those representative chemicals selected. Perturbation of mRNA expressions of GH/IGF axis genes accompanied by decreased body length indicated that indicated that the growth inhibition observed might be attributed to the dysregulation of GH/IGFs axis. Therefore, GH/IGF axis PCR array of zebrafish larvae could be used to evaluate the effects of chemicals on GH/IGF endocrine system.
•Our present study developed GH/IGF axis PCR array of 96-hpf zebrafish embryos/larvae.•Perturbation of mRNA expressions of GH/IGF axis genes accompanied by growth inhibition after exposure to these chemicals.•GH/IGF axis PCR array of 96-hpf zebrafish embryos/larvae could be a reliable tool to identify EDCs.
Traffic signs recognition (TSR) is an important part of some advanced driver-assistance systems (ADASs) and auto driving systems (ADSs). As the first key step of TSR, traffic sign detection (TSD) is ...a challenging problem because of different types, small sizes, complex driving scenes, and occlusions. In recent years, there have been a large number of TSD algorithms based on machine vision and pattern recognition. In this paper, a comprehensive review of the literature on TSD is presented. We divide the reviewed detection methods into five main categories: color-based methods, shape-based methods, color- and shape-based methods, machine-learning-based methods, and LIDAR-based methods. The methods in each category are also classified into different subcategories for understanding and summarizing the mechanisms of different methods. For some reviewed methods that lack comparisons on public datasets, we reimplemented part of these methods for comparison. The experimental comparisons and analyses are presented on the reported performance and the performance of our reimplemented methods. Furthermore, future directions and recommendations of the TSD research are given to promote the development of the TSD.
Searching for efficient electrode materials with excellent electrochemical performance is of great significance to the development of magnesium-ion batteries (MIBs). Two-dimensional Ti-based ...materials are appealing for use in MIBs due to their high cycling capability. On the basis of density functional theory (DFT) calculations, we comprehensively investigate a novel two-dimensional Ti-based material, namely, TiClO monolayer, as a promising anode for MIBs. Monolayer TiClO can be exfoliated from its experimentally known bulk crystal with a moderate cleavage energy of 1.13 J/m
. It exhibits intrinsically metallic properties with good energetical, dynamical, mechanical, and thermal stabilities. Remarkably, TiClO monolayer possesses an ultra-high storage capacity (1079 mA h g
), a low energy barrier (0.41-0.68 eV), and a suitable average open-circuit voltage (0.96 V). The lattice expansion for the TiClO monolayer is slight (<4.3%) during the Mg-ion intercalation. Moreover, bilayer and trilayer TiClO can considerably enhance the Mg binding strength and maintain the quasi-one-dimensional diffusion feature compared with monolayer TiClO. All these properties indicate that TiClO monolayers can be utilized as high-performance anodes for MIBs.
N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) is used as a ubiquitous rubber antioxidant worldwide and has been shown to be potentially toxic to aquatic organisms. In this study, ...zebrafish embryos were exposed to 6PPD for five days starting at two hours post-fertilization at concentrations of 0, 0.0022, 0.022, and 0.22 mg/L to investigate its effects on embryonic development, the growth hormone/insulin-like growth factor (GH/IGF) axis, and the hypothalamic-pituitary-thyroid (HPT) axis. The results showed that the 96 h LC50 of 6PPD was 2.2 mg/L. 6PPD exposure decreased hatchability, lowered autonomous movement, reduced body length in zebrafish embryos and caused deformities. The hormones levels and the expression of genes related to GH/IGF and HPT axis were altered after exposure to 6PPD in zebrafish larvae. These results indicated that the GH/IGF and HPT axis was disturbed. Moreover, treatment of 6PPD produced oxidative stress in zebrafish embryos. Overall, the present study thus demonstrated that exposure to 0.22 mg/L 6PPD caused developmental toxicity and disrupted the GH/IGF and HPT axis of zebrafish, which could be responsible for developmental impairment and growth inhibition.
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•The 96 h LC50 of 6PPD was 2.2 mg/L.•6PPD exposure cause developmental toxicity in zebrafish embryos/larvae.•The developmental toxicity induced by 6PPD is the result of GH/IGF endocrine and thyroid system disruption.•6PPD exposure affected the oxidative stress state of zebrafish embryos/larvae.
► TDCPP exposure altered whole-body thyroid hormones concentrations in zebrafish embryos/larvae. ► The up-regulation of genes related to thyroid hormones metabolism might be responsible for decreased ...thyroxine (T4) concentrations. ► TDCPP exposure up-regulated expression of genes related to thyroid hormones synthesis as a compensatory mechanism for decreased T4 concentrations.
Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) has been frequently detected in the environment and in various biota, including fish, and has been implicated in disruption of the thyroid endocrine system. In the present study, zebrafish (Danio rerio) embryos were exposed to different concentrations of TDCPP (10, 50, 100, 300 and 600μg/L) from 2h post-fertilization (hpf) to 144hpf. Developmental endpoints, and whole-body concentrations of thyroid hormones and transcriptional profiles of genes involved in the hypothalamic–pituitary–thyroid (HPT) axis were examined. Exposure to TDCPP caused a dose-dependent developmental toxicity, including decreased body weight, reduced hatching, survival and heartbeat rates, and increased malformation (spinal curvature). Treatment with the positive control chemical 3,3′,5-triiodo-l-thyronine (T3) significantly decreased whole-body thyroxin (T4) concentrations, increased whole-body T3 concentrations, and upregulated mRNA expression involved in the HPT axis as a compensatory mechanism. These results suggested that the HPT axis in 144-hpf zebrafish larvae was responsive to chemical exposure and could be used to evaluate the effects of chemicals on the thyroid endocrine system. TDCPP exposure significantly decreased whole-body T4 concentrations and increased whole-body T3 concentrations, indicating thyroid endocrine disruption. The upregulation of genes related to thyroid hormone metabolism (dio1 and ugt1ab) might be responsible for decreased T4 concentrations. Treatment with TDCPP also significantly increased transcription of genes involved in thyroid hormone synthesis (tshβ, slc5a5 and tg) and thyroid development (hhex, nkx2.1 and pax8) as a compensatory mechanism for decreased T4 concentrations. Taken together, these results suggest that TDCPP alters the transcription of genes involved in the HPT axis and changes whole-body concentrations of thyroid hormones in zebrafish embryos/larvae, thus causing an endocrine disruption of the thyroid system.
Accelerators for machine learning artificial intelligence (AI) inferencing applications are homogeneous designs composed of identical cores. Each core or processing element (PE) contains ...multiply-and-accumulate units, control logic, and registers for storing and forwarding weights and activations. Testing homogeneous array-based AI accelerator chips by running automatic test pattern generation (ATPG) at the array level results in a high CPU time and pattern count. We propose a constant-testable (C-testable) method for test generation at the PE level such that the ATPG effort does not increase with the number of PEs. Our results show that compared to the traditional array-level testing, the proposed method achieves up to <inline-formula> <tex-math notation="LaTeX">4.2\times </tex-math></inline-formula> (<inline-formula> <tex-math notation="LaTeX">3.5\times </tex-math></inline-formula>), <inline-formula> <tex-math notation="LaTeX">1530\times </tex-math></inline-formula> (<inline-formula> <tex-math notation="LaTeX">2388\times </tex-math></inline-formula>), and <inline-formula> <tex-math notation="LaTeX">170\times </tex-math></inline-formula> (<inline-formula> <tex-math notation="LaTeX">142\times </tex-math></inline-formula>) reduction in the test pattern count, ATPG runtime, and test cycle count, respectively, for stuck-at (transition) faults in a <inline-formula> <tex-math notation="LaTeX">256\times 256 </tex-math></inline-formula> array, while preserving the test coverage. A reconfigurable scan architecture is introduced to enable the proposed C-testable solution for the entire accelerator array. The design-space exploration of a hierarchical test-compaction framework is presented. We also describe four debug solutions for fault localization and diagnosis.
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