Abstract
In order to improve the equalization control efficiency of the battery charge equalization system, this paper applies the double closed-loop controller to the battery charge automatic ...equalization system. First, the overall block diagram of the hardware circuit of the charging system is designed, and the switching control of charging and discharging is completed by using TMS320F2812 chip. Secondly, the optimal charging current is calculated according to Mars law. Then, a double closed-loop controller is introduced to balance the battery charge, and the charge balance equation is constructed and solved. Finally, the adjusted parameters are obtained to realize the automatic charging equalization control. The experimental results show that the equalization control time of this method is always within 25s, which indicates that its charge equalization control effect is good.
Hexokinase II (HK2), a key enzyme involved in glucose metabolism, is regulated by growth factor signaling and is required for initiation and maintenance of tumors. Here we show that metabolic stress ...triggered by perturbation of receptor tyrosine kinase FLT3 in non-acute myeloid leukemia cells sensitizes cancer cells to autophagy inhibition and leads to excessive activation of chaperone-mediated autophagy (CMA). Our data demonstrate that FLT3 is an important sensor of cellular nutritional state and elucidate the role and molecular mechanism of CMA in metabolic regulation and mediating cancer cell death. Importantly, our proteome analysis revealed that HK2 is a CMA substrate and that its degradation by CMA is regulated by glucose availability. We reveal a new mechanism by which excessive activation of CMA may be exploited pharmacologically to eliminate cancer cells by inhibiting both FLT3 and autophagy. Our study delineates a novel pharmacological strategy to promote the degradation of HK2 in cancer cells.
Hepatocellular carcinoma (HCC) is believed to arise from tumor-initiating cells (T-ICs), which are responsible for tumor relapse and metastases. Portal vein tumor thrombus (PVTT) is raised from HCC ...and strongly correlated to a poor prognosis. However, the mechanism underling the formation of PVTT is largely unknown. Herein, we provide evidence that RNA polymerase II subunit 5 (RPB5)-mediating protein (RMP) was progressively upregulated in PVTT and overexpressed RMP appeared to increase T-ICs self-renewal. Moreover, RMP promoted metastases of PVTT cells and HCC cells in vitro and in vivo. Knockdown of RMP attenuated T-ICs self-renewal and reversed epithelial-mesenchymal transition (EMT) in HCC and PVTT cells. The neutralizing assays suggested that interleukin-6 (IL-6) had an indispensable role in RMP regulating metastases and self-renewal of HCC cells. Furthermore, the transcription of IL-6 was verified to be modulated by RMP via interaction with p65 and RPB5, through which expanding the T-IC/cancer stem cell populations, as well as inducing EMT was promoted. These results suggested that RMP may promote PVTT formation by promoting IL-6 transcription. Thus, RMP serves as a potent factor contributed to develop PVTT and a promising therapeutic target for HCC patients.
This paper presents a comparative analysis of point clouds acquired from a terrestrial laser scanner (TLS) survey and a 3D virtual tour using Matterport technology for heritage building information ...modeling (HBIM) development. The study uses the Cloister of the Royal College of Corpus Christi Seminary, an important cultural heritage site in Valencia, Spain, as a case study. The point clouds from the TLS survey and Matterport scans were compared both quantitively in CloudCompare software and visually to assess their accuracy and quality. The Matterport point cloud data was found to be slightly lower in quality and accuracy compared to the TLS data, but still sufficient for developing some low-tolerance geometry in the HBIM model. The study shows that Matterport point cloud data has potential to supplement TLS scans, particularly in areas missed during the TLS survey due to range limitations. Matterport technology is accessible, affordable, and easy to use, making it a feasible option for heritage sites with limited resources. Moreover, Matterport technology captures high-quality visual data, including color and texture, providing a detailed representation of the heritage site. Thus, Matterport technology can be a valuable contribution to cultural heritage documentation and preservation, particularly for sites requiring a quick and efficient surveying process. The findings of this research offer insights into the relative advantages and limitations of these two reality capture techniques for cultural heritage documentation and preservation, and could potentially inform decision-making processes for future heritage preservation projects.
Aim
This study aimed to understand the role of miR‐133a in progesterone actions, explore the regulative mechanism of the progesterone receptor, and investigate the effects of miR‐133a on the ...progesterone‐inhibited proliferation of mouse endometrial epithelial cells.
Methods
The expression of miR‐133a induced by progesterone was detected by quantitative real‐time PCR both in vivo and in vitro. Ishikawa subcell lines stably transfected with progesterone receptor subtypes were used to determine the receptor mechanism of progesterone inducing miR‐133a. Specific miR‐133a mimics or inhibitors were transfected into mouse uteri and primary cultured endometrial epithelial cells to overexpress or downregulate the miR‐133a. The roles of miR‐133a in the cell cycle and proliferation of endometrial epithelial cells were analysed by flow cytometry and Edu incorporation analysis. The protein levels of cyclinD2 in uterine tissue sections and primary cultured endometrial epithelial cells were determined by immunohistochemistry and Western blot analysis.
Results
Progesterone could induce miR‐133a expression in a PRB‐dependent manner in endometrial epithelial cells. miR‐133a inhibited endometrial epithelial cell proliferation by arresting cell cycle at the G1‐S transition. Moreover, miR‐133a acted as an inhibitor in downregulating cyclinD2 in endometrial epithelial cells.
Conclusion
We showed for the first time that progesterone‐induced miR‐133a inhibited the proliferation of endometrial epithelial cells by downregulating cyclinD2. Our research indicated an important mechanism for progesterone inhibiting the proliferation of endometrial epithelial cells by inducing special miRNAs to inhibit positive regulatory proteins in the cell cycle.
Objectives
Early recognition of coronavirus disease 2019 (COVID-19) severity can guide patient management. However, it is challenging to predict when COVID-19 patients will progress to critical ...illness. This study aimed to develop an artificial intelligence system to predict future deterioration to critical illness in COVID-19 patients.
Methods
An artificial intelligence (AI) system in a time-to-event analysis framework was developed to integrate chest CT and clinical data for risk prediction of future deterioration to critical illness in patients with COVID-19.
Results
A multi-institutional international cohort of 1,051 patients with RT-PCR confirmed COVID-19 and chest CT was included in this study. Of them, 282 patients developed critical illness, which was defined as requiring ICU admission and/or mechanical ventilation and/or reaching death during their hospital stay. The AI system achieved a C-index of 0.80 for predicting individual COVID-19 patients’ to critical illness. The AI system successfully stratified the patients into high-risk and low-risk groups with distinct progression risks (
p
< 0.0001).
Conclusions
Using CT imaging and clinical data, the AI system successfully predicted time to critical illness for individual patients and identified patients with high risk. AI has the potential to accurately triage patients and facilitate personalized treatment.
Key Point
• AI system can predict time to critical illness for patients with COVID-19 by using CT imaging and clinical data.
The process e^{+}e^{-}→D_{s}^{*+}D_{s}^{*-} is studied with a semi-inclusive method using data samples at center-of-mass energies from threshold to 4.95 GeV collected with the BESIII detector ...operating at the Beijing Electron Positron Collider. The Born cross sections of the process are measured for the first time with high precision in this energy region. Two resonance structures are observed in the energy-dependent cross sections around 4.2 and 4.4 GeV. By fitting the cross sections with a coherent sum of three Breit-Wigner amplitudes and one phase-space amplitude, the two significant structures are assigned masses of (4186.8±8.7±30) and (4414.6±3.4±6.1) MeV/c^{2}, widths of (55±15±53) and (122.5±7.5±8.1) MeV, where the first errors are statistical and the second ones are systematic. The inclusion of a third Breit-Wigner amplitude is necessary to describe a structure around 4.79 GeV.
Previous research has shown that stacking unidirectional laminates helicoidally with small interply angles resulted in improvements in transverse load resistance. Guided by computational simulations, ...damage evolution within helicoidal and cross-ply laminates was tracked with further experiments to offer an insight into key differences responsible for their distinct load bearing characteristics. Under transverse loads, the first form of damage is delamination. Unlike cross-plys, which suffer multiple delamination of about the same size throughout the thickness, delamination is harder to initiate in helicoidal laminates due to the small angle between each ply. A large delamination eventually formed at the mid-plane. Transverse cracks then appear on the tensile surface of both types of specimens and propagate upwards with increasing loads. Load drops occur whenever the transverse cracks propagate to the delamination immediately above them. In the case of cross-plys, where there are multiple delamination, multiple load drops occur. Load drops in helicoidal laminates are delayed until the cracks reach the large delamination in the mid-plane. Helicoidal specimens do not experience multiple loads drops and can attain high peak load before catastrophic failure. It is shown that even higher peak load is achieved by selectively seeding delamination in helicoidal laminates to further delay the merging of transverse cracks with the dominant delamination.
Space charge effect of intense ion beam has critical influence on separation efficiency in the electromagnetic isotope separator. In this paper, a ribbon beam extraction system with slit electrodes ...for an electromagnetic isotope separator was designed and studied. The extracted beam currents were varied from 10 to 40 mA and the corresponding extracted beam energies were 40 keV and 100 keV respectively. The simulated output beam density distributions were used in the subsequent multi species particle transmission simulation with the space charge effect included. The separated isotope beam spot distributions at the focal plane were simulated under different space charge compensation factors and thus the optimum operation gas pressures in vacuum box were roughly estimated. For the case of high intensity and high power isotope beam collection, an isotope collector with a deceleration electrode was proposed to mitigate the effect of high power beam bombardment and the resultant temperature rising on the collector surface.
•The ribbon beam extraction systems were simulated for an electromagnetic isotope separator.•The influence of space charge effect on isotopes separation efficiency was studied via numerical simulations.•The optimal residual gas pressure for fully beam space charge neutralization was roughly estimated.
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