The value of the "touching-bone" acupuncture technique in clinical application was explained through the investigation on the origin of the theory of the "touching-bone" acupuncture technique, the ...analysis on the characteristics of acupoint selection, the introduction of clinical characteristics and the discussion on the mechanism of acupuncture in treatment. The "touching-bone" acupuncture technique refers to deep needling method, originated from the short needling and
needling of the ancient needling methodslisted in the
. The target points are the reaction sites on meridian near to bone and the attachments of soft tissues on bone. During the needle insertion, the needle tip is thrust deeply to the bone or the needle body is closely attached to the bone so as to stimulate periosteum specifically. This needling technique contributes to the satisfactory effect on spasmodic, deep-located and intractable pain disorder, motor system diseases, mental diseases and cerebral diseases, etc. Hence, this acupuncture te
Background： Successful antiretroviral therapy （ART） has been demonstrated to be effective in reducing the infectivity of human immunodeficiency virus （HIV）. We conducted a study to predict the ...potential effect of ART on the spread of HIV in Chaoyang District, Beijing, China, using the Asian Epidemic Model （AEM）. Methods： The AEM baseline workbook was used to determine the current infection status and to project the future spread of HIV under current conditions. We changed the input on the ART coverage from 2014 to 2025 and also modified the treatment eligibility in the AEM intervention workbook, in order to allow for analysis of the projected downstream impact of ART. Results： By gradually increasing the ART coverage rate from 29.7% （rate of 2013） to 40.0%, 50.0%, 60.0%, 70.0%, 80.0%, and 90.0% （at CD4＋ ≤350 cells/μl）, and by changing the dates of coverage from 2014 to 2020, the number of new infections showed a cumulative decline of 0.60%, 1.59%, 2.94%, 5.33%, 9.32%, and 14.98%, respectively. After 2020, the projected rates of infection rebounded slightly, so with the exception of the years with very high coverage （90.0%）, new infections continued to decrease. When we changed the initial threshold of therapy to CD4＋ cell counts ≤500 cells/μl, new infections decreased 6.00%, 11.64%, 15.92%, 21.11%, 26.92%, 33.05%, and 38.75%, respectively, under varying ART coverages. Conclusion： Our study demonstrates that the early initiation of ART for people living with HIV/acquired immune deficiency syndrome （AIDS） has a positive effect in slowing the spread of HIV.
In order to have a better understanding about the role of the extracellular matrix molecules in the intermittent and constant exotropia, we measured the amounts of fibronectin and proteoglycan in the ...resected medial rectus muscles of patients with concomitant exotropia.
Thirty-one exotropic patients (including intermittent exotropia 17 cases and constant exotropia 14 cases) and 21 normal were chosen. In exotropic group, there were 7 cases with positive family history. Tissues of the medial rectus muscles were obtained from patients with concomitant exotropia during resection surgery and the specimen of medial rectus muscle from 21 normal persons was as control. All the tissues were weighted and then pulverized to obtain supernatant for enzyme linked immunosorbent assay (ELISA). The total amounts of fibronectin and proteoglycan were measured. The correlation of fibronectin and proteoglycan with age, gender, positive family history in different groups of exotropia was analyzed.
The amount of fibronectin in the
To establish a hepatoma specific murine IL-1beta (mIL-1beta) expression vector operated by AFP promoter and analysis its expression in H22 cell.
The chimeric operating sequence composed of the ...minimal AFP promoter and CMV enhancer(ECMV) was prepared through SOE-PCR. The sequence was inserted to replace the conventional enhancer and promoter in pIRES2-EGFP to establish the novel hepatoma specific vector p(afp)IRES2-EGFP. Full length of murine IL-1beta was amplified through RT-PCR by pfu DNA polymerase followed by cloning to establish the recombinant pIRES2-EGFP-mIL-1beta expression vector verified through PCR, restriction enzyme assay, DNA sequencing and cell transfection. p(afp)IRES2-EGFP-mIL-1beta was tranfected into H22 hepatoma cells and YAC-1 lymphoma cells in a transient transfection system mediated by jetPEI. Expression of the vector was observed under fluorescent microscope 48 h after transfection. Expression level of mIL-1beta was detected by RT-PCR.
A 537 bp chimeric AFP promoter and ECMV was yield a
Abstract
A family of monodisperse YF
3
, YF
3
:Ce
3+
and YF
3
:Ce
3+
/Ln
3+
(Ln=Tb, Eu) mesocrystals with a morphology of a hollow spindle can be synthesized by a solvothermal process using yttrium ...nitrate and NH
4
F as precursors. The effects of reaction time, fluorine source, solvents, and reaction temperature on the synthesis of these mesocrystals have been studied in detail. The results demonstrate that the formation of a hollow spindle‐like YF
3
can be ascribed to a nonclassical crystallization process by means of a particle‐based reaction route in ethanol. It has been shown that the fluorine sources selected have a remarkable effect on the morphologies and crystalline phases of the final products. Moreover, the luminescent properties of Ln
3+
‐doped and Ce
3+
/Ln
3+
‐co‐doped spindle‐like YF
3
mesocrystals were also investigated. It turns out that Ce
3+
is an efficient sensitizer for Ln
3+
in the spindle‐like YF
3
mesocrystals. Remarkable fluorescence enhancement was observed in Ce
3+
/Ln
3+
‐co‐doped YF
3
mesocrystals. The mechanism of the energy transfer and electronic transition between Ce
3+
and Ln
3+
in the host material of YF
3
mesocrystals was also explored. The cytotoxicity study revealed that these YF
3
‐based nanocrystals are biocompatible for applications, such as cellular imaging.
A family of monodisperse YF sub(3), YF sub(3):Ce super(3+) and YF sub(3):Ce super(3+)/Ln super(3+) (Ln=Tb, Eu) mesocrystals with a morphology of a hollow spindle can be synthesized by a solvothermal ...process using yttrium nitrate and NH sub(4)F as precursors. The effects of reaction time, fluorine source, solvents, and reaction temperature on the synthesis of these mesocrystals have been studied in detail. The results demonstrate that the formation of a hollow spindle-like YF sub(3) can be ascribed to a nonclassical crystallization process by means of a particle-based reaction route in ethanol. It has been shown that the fluorine sources selected have a remarkable effect on the morphologies and crystalline phases of the final products. Moreover, the luminescent properties of Ln super(3+)-doped and Ce super(3+)/Ln super(3+)-co- doped spindle-like YF sub(3) mesocrystals were also investigated. It turns out that Ce super(3+) is an efficient sensitizer for Ln super(3+) in the spindle-like YF sub(3) mesocrystals. Remarkable fluorescence enhancement was observed in Ce super(3+)/Ln super(3+)-co- doped YF sub(3) mesocrystals. The mechanism of the energy transfer and electronic transition between Ce super(3+) and Ln super(3+) in the host material of YF sub(3) mesocrystals was also explored. The cytotoxicity study revealed that these YF sub(3)-based nanocrystals are biocompatible for applications, such as cellular imaging. Spindle-like YF sub(3), YF sub(3):Ce super(3+) and YF sub(3):Ce super(3+)/Ln super(3+) (Ln=Tb, Eu) mesocrystals have been synthesized by a robust solvothermal route. The luminescent properties of these mesocrystals were carefully investigated and the cytotoxicity study revealed that these YF sub(3)-based nanocrystals are biocompatible for bio-applications such as cellular imaging.
Electromagnetic acoustic transducers (EMATs) have been applied to generate guided waves. The type of guided waves in steel plates is transformed as the bias angle of horizontal magnetic field varies. ...A simple analytical model to predict three transduction mechanisms including Lorentz, magnetization and magnetostriction force has been put forward in this paper. Three coefficients were determined to explain the effect. The experiments on single-coil EMAT presented the echo amplitude distribution of SH guided waves and Lamb waves. It shows that 40° and 70° are the type transition angles. The most efficient generation angle for Lamb waves is 90°, but that for SH guided waves is dependent on the field magnitude.
In attempts to delay tumor progression after surgery or minimally invasive local treatments, multidisciplinary strategies have been broadly studied in patients with hepatocellular carcinoma (HCC). ...The objective of this present study was to evaluate the efficacy of autologous transplantations of cytokine-induced killer (CIK) cells as an adjuvant therapy for patients with HCC. A total of 264 patients with HCC were enrolled in this retrospective study. Of these patients, 165 received either CIK cell therapy alone or as adjuvant therapy to surgery, transcatheter arterial chemoembolization (TACE), or TACE-based comprehensive treatments (CT). The remaining 99 patients received only surgery or TACE. Kaplan-Meier analysis and the Chi-squared test were used to analyze the overall survival (OS), progression-free survival (PFS), and clinical characteristics of the patients in the different treatment subgroups. Kaplan-Meier analysis suggested that patients in the Surgery+CIK group had a significantly improved OS compared with those in the other three groups (P < 0.001). Furthermore, patients who developed a fever after the CIK cell treatments manifested a likely better OS (P = 0.028). Subgroup analysis indicated that patients in the Surgery+CIK group likely had an improved PFS but a similar OS compared with the patients in the Surgery-alone group (P = 0.055 for PFS, and P = 0.746 for OS). Further subgroup analysis showed that the OS in both the TACE+CIK and CT+CIK groups was prolonged significantly compared with that in the TACE-alone group (P = 0.015 and P = 0.018, respectively). However, similar OS was observed between the TACE+CIK and CT+CIK groups (P = 0.686). Autologous transplantation of CIK cells as an adjuvant therapy was associated with better survival for patients with HCC, especially for those who had also undergone TACE. A fever reaction might be a potential event for assessing the curative effect of the CIK treatment.
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