Mesenchymal stem/stromal cells （MSCs） possess some characteristics of immune cells, including a pro-inflammatory phenotype, an immunosuppressive phenotype, antibacterial properties and the expression ...of Toll-like receptor proteins. Here we show that, similar to immune cells, MSCs retain information from danger signals or environmental stimuli for a period of time. When treated with the pro-inflammatory factors lipopolysaccharide （LPS） or tumor necrosis factor-a （TNF-a）, MSCs display increased expression of IL-6, IL-8 and MCP-1. Following re-plating and several rounds of cell division in the absence of stimulating factors, the expression of IL-6, IL-8 and MCP-1 remained higher than in untreated cells for over 7 days. A spike in cytokine secretion occurred when cells were exposed to a second round of stimulation. We primed MSCs with LPS and LPS-primed MSCs had better therapeutic efficacy at promoting skin flap survival in a diabetic rat model than did unprimed MSCs. Finally, we found that several microRNAs, including miR146a, miR150 and miR155, along with the modification of DNA by 5-hydroxymethylcytosine （5hmC）, mediate the MSC response to LPS and TNF-e stimulation. Collectively, our data suggest that MSCs have a short-term memory of environmental signals, which may impact their therapeutic potential.
The double-strand DNA (dsDNA) can act as an efficient template for the formation of copper nanoparticles (Cu NPs) with high fluorescence, whereas the single-strand DNA (ssDNA) cannot support the ...formation of Cu NPs. This difference in fluorescent signal generation can be used for the detection of nuclease cleavage activity. Thus, a label-free strategy for sensitive detection of nuclease has been developed. The sensor contains a complete complementary dsDNA which acts as a template for the formation of Cu NPs and generation of fluorescence signal. The enzyme S1 nuclease was taken as the model analyte. Upon addition of S1 nuclease into the sensing system, the DNA was cleaved into fragments, preventing the formation of the Cu NPs and resulting in low fluorescence. In order to achieve the system's best sensing performance, a series of experimental conditions were optimized. Under the optimized experimental conditions, the sensor exhibits excellent performance (e.g., a detection limit of 0.3UmL−1 with high selectivity). This possibly makes it an attractive platform for the detection of S1 nuclease and other biomolecules.
► A fluorescent probe based on double-strand DNA-templated formation of copper nanoparticles was developed for label-free nuclease enzyme detection. ► The probe shows a high sensitivity to S1 nuclease activity with a low detection limit of 0.3UmL−1 observed. ► It also exhibits high selectivity to S1 nuclease over other nucleases.
To investigate the substrate specificity of mouse aldo-keto reductase AKR7A5 protein towards naphthoquinone and its derivatives.
The recombinant His-tagged AKR7A5 fusion protein in E.coli BL21pLysS ...cell strain was induced by IPTG and purified using FPLC system through HiTrap affinity column. The purified recombinant AKR7A5 protein was confirmed by SDS-PAGE and Western blotting. AKR enzyme assay was applied to measure the substrate specificity of recombinant AKR7A5 protein towards naphthoquinone and its derivatives.
Recombinant His-AKR7A5 was successfully purified as confirmed by SDS-PAGE and Western blotting. AKR enzyme assay indicated that the recombinant AKR7A5 protein exhibited mild substrate specificity towards lawsone and low specificity towards juglone and vitamine K3, but no activity towards 1, 4-naphthoquinone.
AKR7A5 has selective substrate specificity towards naphthoquinone derivatives, suggesting that the aldo-keto reductase could play an important role in metabolism of certain naphthoquinone deriv
Two new neolignans, (7S,8R)-guaiacylglycerol-8-O-4'-sinapyl ether 9'-O-beta-D- glucopyranoside (1) and (7S,8R)-syringylglycerol-8-O-4'-sinapyl ether 9'-O-beta-D-gluco- pyranoside (2) were isolated ...from the leaves of Syringa velutina Kom.. Their structures were established by chemical properties and spectroscopic evidence.
Alcohol use disorder (AUD) is a common mental illness with high morbidity and disability. The discovery of laboratory biomarkers has progressed slowly, resulting in suboptimal diagnosis and treatment ...of AUD. This study aimed to identify promising biomarkers, as well as the potential miRNA-mRNA networks associated with AUD pathogenesis. RNA sequencing was performed on plasma-derived small extracellular vesicles (sEVs) from AUD patients and healthy controls (HCs) to harvest miRNAs expression profiles. Machine learning (ML) models were built to screen characteristic miRNAs, whose target mRNAs were analyzed using TargetScan, miRanda and miRDB databases. Gene Expression Omnibus (GEO) datasets (GSE181804 and GSE180722) providing postmortem hippocampal gene expression profiles of AUD subjects were mined. A total of 247 differentially expressed (DE) plasma-derived sEVs miRNAs and 122 DE hippocampal mRNAs were obtained. Then, 22 overlapping sEVs miRNAs with high importance scores were gained by intersecting 5 ML models. As a result, we established a putative sEVs miRNA-hippocampal mRNA network that can effectively distinguish AUD patients from HCs. In conclusion, we proposed 5 AUD-representative sEVs miRNAs (hsa-miR-144-5p, hsa-miR-182-5p, hsa-miR-142-5p, hsa-miR-7-5p, and hsa-miR-15b-5p) that may participate in the pathogenesis of AUD by modulating downstream target hippocampal genes. These findings may provide novel insights into the diagnosis and treatment of AUD.
To establish an HPLC method for the simultaneous determination of four bioactive cucurbitacins in Cucubita pepo cv Dayangua.
The HPLC chromatography was carried out with a lineal gradient programming ...and detection wavelength at 215 nm. Kromasil C8 column (150 mm x 4.6 mm ID, 5 microm)was used. The mobile phase was acetonitrile-water (containing 2.0% HAc) and the flow rate was 1.0 ml/min.
The linear range of 23, 24-dihydrocucurbitacin F was 0.28-5.6 microg/ml (r = 0.9978), 23, 24-dihydrocucurbitacin D 0.39-7.8 microg/ml (r = 0.9986), cucurbitacin B 0.304-6.08 microg/ml (r = 0.9983), cucurbitacin E 2. 52 -50. 4 microg/ml (r = 0.9998). The method was accurate with variation less than 5% and recovery more than 95%.
The method is successfully applied to determination of the four cucurbitacins from Cucubita pepo cv dayangua.
AIM：To assess the corneal sensitivity and the incidences of dry eye after small incision lenticule extraction（SMILE） and femtosecond laser-assisted in situ keratomileusis（FSLASIK）.METHODS：The ...Meta-analysis was performed using Rev Man 5.3.We searched on Pub Med from inception to March 2016.Summary weighted mean difference（WMD） and 95% confidence intervals（CIs） were used to analyze the datum.Random-effects or fixed-effects models were chosen up to between-study heterogeneity.The main outcomes were composed of the Ocular Surface Disease Index（OSDI） scores,tear film break-up time（TBUT）,Schirmer Test and corneal sensitivity.RESULTS：Eight eligible studies including 772 eyes（386 in SMILE group and 386 in FS-LASIK group） were identified.The parameters have no significiant difference heterogeneity between SMILE and FS-LASIK group preoperatively.There were significant differences between the two groups in OSDI scores at one and three months postoperatively,in TBUT at one and three months postoperatively,in corneal sensitivity at one week,about one month and three months postoperatively.However,there was no significant difference observed in Schirmer Test at the follow-up periods.CONCLUSION：Compare to FS-LASIK,dry eye and the corneal sensitivity recover better in the SMILE group,in first three months after the surgery.
This study aims to explore the mechanism of Liujunzi Decoction in the treatment of 4-nitroquinoline-N-oxide(4NQO)-induced esophageal cancer in mice. One hundred mice of 35-45 days were randomized ...into blank, model, and low-, medium-, and high-concentration(18.2, 36.4, and 54.6 g·kg~(-1), respectively) Liujunzi Decoction groups. The mice in other groups except the blank group had free access to the water containing 100 μg·mL~(-1) 4NQO for 16 weeks for the modeling of esophageal cancer. The mice in the Liujunzi Decoction groups were fed with the diets supplemented with corresponding concentrations of Liujunzi Decoction. The body weight and organ weights were weighed for the calculation of organ indexes. The pathological changes of the esophageal tissue were observed by hematoxylin-eosin(HE) staining. Ultra performance liquid chromatography-mass spectrometry(UPLC-MS/MS) was employed to collect metabolites from mouse serum samples, screen out potential biomarkers, and predict related metabolic pathways. Compared
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