Piperacillin antibody-induced immune hemolytic anemia is not rare in adults, and there have been reports of anti-HLA antibody-induced newborn platelet transfusion refractoriness. However, there has ...been no report of piperacillin-accompanied anti-HLA antibody-induced newborn pancytopenia.
We herein present the case of a newborn with pancytopenia from a mother who carried anti-HLA-B55, anti-HLA-DR11, and piperacillin antibodies. The newborn HLA genotypes were HLA*B55:02 and HLA*DRB1*11:01. IgG antibodies can be transferred to the newborn via the placenta and induce the destruction of the platelet and white blood cells, which carry the corresponding antigens. Piperacillin antibodies coupling with newborn red blood cells (RBCs) led to the destruction of the RBCs and hemolytic anemia.
The direct anti-globulin test was positive for RBCs in the newborn, and piperacillin antibodies were positive in both the newborn and his mother. Anti-HLA antibodies were positive in the maternal serum, whereas homologous antigens were positive in the newborn. The direct anti-globulin test of platelet was weekly positive in the newborn.
Piperacillin and anti-HLA antibodies can pass through the placenta, induce incompatible blood cell destruction, and cause a series of clinical syndromes in newborns.
Most small diameter vascular grafts (inner diameter<6 mm) evaluation studies are performed in healthy animals that cannot represent the clinical situation. Herein, an hypercholesterolemia (HC) rat ...model with thickened intima and elevated expression of pro-inflammatory intercellular adhesion molecular-1 (ICAM-1) in the carotid branch is established. Electrospun polycaprolactone (PCL) vascular grafts (length: 1 cm; inner diameter: 2 mm) are implanted into the HC rat abdominal aortas in an end to end fashion and followed up to 43 days, showing a relative lower patency accompanied by significant neointima hyperplasia, abundant collagen deposition, and slower endothelialization than those implanted into healthy ones. Moreover, the proliferation, migration, and adhesion behavior of endothelial cells (ECs) isolated from the HC aortas are impaired as evaluated under both static and pulsatile flow conditions. DNA microarray studies of the HC aortic endothelium suggest genes involved in EC proliferation (Egr2), apoptosis (Zbtb16 and Mt1), and metabolism (Slc7a11 and Hamp) are down regulated. These results suggest the impaired proliferative, migratory, and adhesive abilities of ECs are associated with the bad performances of grafts in HC rat. Future pre-clinical evaluation of small diameter vascular grafts may concern more disease animal models with clinical complications.
During the development of small diameter vascular grafts (D<6 mm), young and healthy animal models from pigs, sheep, dogs, to rabbits and rats are preferred. However, it cannot represent the clinic situation, where most cardiovascular grafting procedures are performed in the elderly and age is the primary risk factor for disease development or death. Herein, the performance of electrospun polycaprolactone (PCL) vascular grafts implanted into hypercholesterolemia (HC) or healthy rats were evaluated. Results suggest the proliferative, migratory, and adhesive abilities of endothelial cells (ECs) are already impaired in HC rats, which contributes to the observed slower endothelialization of implanted PCL grafts. Future pre-clinical evaluation of small diameter vascular grafts may concern more disease animal models with clinical complications.
Display omitted
Thermal barrier coatings (TBCs) suffer from the thermo-chemo-mechanical coupling action of thermal shock and calcium–magnesium–alumina–silicate (CMAS) corrosion. However, the failure mechanism of ...TBCs under the synergistic effect of thermal shock and CMAS corrosion is still unclear due to a lack of an environmental simulator. Herein, an 8YSZ ceramic coating is deposited on a PtAl bond coating/DD419 nickel-based single crystal superalloy substrate using the electron beam physical vapor deposition (EB-PVD) method. The thermo-chemo-mechanical coupling effect of TBCs is achieved in a self-developed environmental simulator. The interaction of volume expansion induced by the phase transition of ZrO2, structural degradation and thermal fatigue further increases the out-of-plane tensile stress and in-plane shear stress in the ceramic coating, which accelerates the initiation and propagation of surface vertical cracks and horizontal cracks. As multiple surface vertical cracks propagate to the interface and merge with interfacial cracks, the ceramic coating spalls from the substrate.
The molecular landscapes of diffuse large B-cell lymphoma (DLBCL) remained to be comprehensively investigated with an urgent need to identify novel prognostic biomarkers guiding prognostic ...stratification and disease monitoring. Baseline tumor samples of 148 DLBCL patients were analyzed using targeted next-generation sequencing (NGS) for mutational profiling, whose clinical reports were retrospectively reviewed. In this cohort, the subgroup of old DLBCL patients (age at diagnosis > 60,
N
= 80) exhibited significantly higher Eastern Cooperative Oncology Group scores and International Prognostic Index than their young counterparts (age at diagnosis ≤ 60,
N
= 68). As revealed by the NGS results,
PIM1
(43.9%),
KMT2D
(31.8%),
MYD88
(29.7%), and
CD79B
(27.0%) were identified as the most frequently mutated genes. Aberrations of genes of the immune escape pathway were significantly enriched in the young subgroup, while the altered epigenetic regulators were more abundant in the old patients.
FAT4
mutation was identified as a positive prognostic biomarker, associated with longer progression-free survival and overall survival in the entire cohort and the old subgroup, using the Cox regression analyses. However, the prognostic function of
FAT4
was not reproduced in the young subgroup. We comprehensively analyzed the pathological and molecular characteristics of old and young DLBCL patients and demonstrated the prognostic value of
FAT4
mutation, which requires further validation with sizable cohorts in future research.
It has long been puzzling that water nanodroplets undergo simultaneously “supercooling” at freezing and “superheating” at melting. Recent progress (Sun et al. J. Phys. Chem. Lett. 2013, 4, 2565, ...3238) enables us to resolve this anomaly from the perspective of hydrogen bond (O:H–O) specific heat disparity. A superposition of the specific heat η x (T) curves for the H–O bond (x = H) and the O:H nonbond (x = L) defines two intersecting temperatures that form the ice/quasi-solid/liquid phase boundaries. Molecular undercoordination (with fewer than four nearest neighbors in the bulk) stretches the ηH(T) curve by raising the Debye temperature ΘDH through H–O bond shortening and phonon stiffening. The ηH(T) stretching is coupled with the ηL(T) depressing because of the Coulomb repulsion between electron pairs on oxygen ions. The extent of dispersion varies with the size of a droplet that prefers a core–shell structure configurationthe bulk interior and the skin. Understandings may open an effective way of dealing with the thermodynamic behavior of water droplets and bubbles from the perspective of O:H–O bond cooperativity.
Room-temperature phosphorescence (RTP) carbon dots (CDs) materials have garnered considerable attention from researchers because of their potential applications in anti-counterfeiting. However, the ...in situ preparation of CDs-based RTP materials with long lifetime and excellent stabilitie remains a considerable challenge. Herein, an in situ manufacturing strategy is proposed to fabricate CDs@Allophane core–shell nanoparticles with double confinement via a simple one-step calcination method. The double confinement arises due to the formation of strong covalent bonds and highly rigid structures via calcination. This double confinement mechanism effectively suppresses the vibration of light-emitting molecules, nonradiative transition of triplet excitons, and environment-induced quenching, affording CDs@Allophane materials with a 1.02 s long lifetime (more than 14 s to the naked eye) and excellent stability. These composites can be preliminarily applied in anti-counterfeiting. This study provides a design approach for the in situ manufacturing of CDs-based RTP materials with a long lifetime and high stability.
The process and mechanism of gold mineralization are frontier issues. The Dunbasitao deposit is the most important gold deposit discovered along the Armantai suture zone, East Junggar, NW China, ...which indicates the potential for future ore exploration in this area. Orebodies are mainly hosted in Lower Carboniferous Jiangbasitao Formation volcano-sedimentary rocks, and the ores are characterized by multistage pyrites. Based on microscopy and backscattered electron imaging studies, pyrites are classified into five types: the pre-ore framboidal/colloidal Py0; the early-stage coarse-grained, cubic, and homogeneous Py1; and the middle-stage fine-grained, cubic/pyritohedron Py2 that includes Py2a (core), Py2b (mantle), and Py2c (rim). The results of the EPMA and in situ LA-ICP-MS analyses show that trace elements of pyrite mainly occur in two forms: solid solutions and invisible or visible inclusions. Mn, Co, Ni, and As enter the pyrite lattice, whereas Ti occurs as mineral inclusions, and Au, Cu, Zn, Sb, and Pb can occur in both forms. Au and As show a positive linear relationship with r = 0.850. Py2b has much higher Au contents (20.1 to 201 ppm) than other pyrite types (Py0: 0.01 to 0.36 ppm; Py1: 0.01 to 0.02 ppm; Py2a: 0.31 to 2.48 ppm; and Py2c: 0.18 to 18.0 ppm). The Dunbasitao deposit is classified as an orogenic gold deposit using the two latest machine learning classifiers based on pyrite trace element data. Fluid immiscibility, sudden cooling, and the substitution of S1− with As1− might be crucial mechanisms leading to Au precipitation. Initial ore-forming fluids brought major amounts of As, Au, Co, Ni, Se, Zn, Ag, Cd, Sn, and other elements, and the Jiangbasitao Formation host rocks contributed a certain amount of As, Ni, Cu, Sb, Pb, and Bi, at least.
Here we demonstrate that "concentration polarization" of ox-LDL enhances LOX-1 expression and ox-LDL uptake. It damages cell surface heparan sulfate proteoglycans (HSPG) and activates LOX-1 dependent ...autophagy and apoptosis. We also show that ox-LDL concentration polarization occurs on the surface of rabbit thoracic aorta and induces autophagy and apoptosis. In order to investigate the significance of swirling flow on LOX-1 expression, HSPG damage, autophagy and apoptosis in the arterial system, an ex vivo model of swirling flow was developed. We observed that swirling flow decreases relative wall concentration of ox-LDL, inhibits LOX-1 expression, protects HSPG from damage, and decreases both autophagy and apoptosis. Taken together, our data suggest that ox-LDL concentration polarization plays an important role in the localization of atherosclerotic lesions concomitant with LOX-1 dependent autophagy and apoptosis. These observations also suggest a novel mechanism by which swirling flow in the arterial system protects arterial wall from atherogenesis.
PDF
