To explore the characteristics of Helicobacter pylori resistance in China and the association between antibiotic resistance and several clinical factors.
H. pylori strains were collected from ...patients in 13 provinces or cities in China between 2010 and 2016. Demographic data including type of disease, geographic area, age, gender and isolation year were collected to analyse their association with antibiotic resistance. Antibiotic resistance was detected using the Etest test and the Kirby-Bauer disc diffusion method.
H. pylori were successfully cultured from 1117 patients. The prevalence of metronidazole, clarithromycin (CLA), azithromycin, levofloxacin (LEV), moxifloxacin, amoxicillin (AMO), tetracycline and rifampicin resistance was 78.2, 22.1, 23.3, 19.2, 17.2, 3.4, 1.9 and 1.5%, respectively. No resistance to furazolidone was observed. The resistance rates to LEV and moxifloxacin were higher in strains isolated from patients with gastritis compared to those with duodenal ulcer and among women. Compared to patients ≥40 years old, younger patients exhibited lower resistance rates to CLA, azithromycin, LEV and moxifloxacin. The resistance rates to CLA and AMO were higher in strains isolated more recently, and we also found that the prevalence of resistance to metronidazole, CLA, azithromycin and AMO were significantly different among different regions of China.
The resistance rates to metronidazole, CLA and LEV were high in China. Patient age, gender, disease and location were associated with the resistance of H. pylori to some antibiotics. Furazolidone, AMO and tetracycline are better choices for H. pylori treatment in China.
Elastic neutrino scattering on electrons is a precisely known purely leptonic process that provides a standard candle for measuring neutrino flux in conventional neutrino beams. Using a total sample ...of 810 neutrino-electron scatters after background subtraction, the measurement reduces the normalization uncertainty on the ν μ NuMI beam flux between 2 and 20 GeV from 7.6 to 3.9%. This is the most precise measurement of neutrino-electron scattering to date, will reduce uncertainties on MINER ν A's absolute cross section measurements, and demonstrates a technique that can be used in future neutrino beams such as long baseline neutrino facility.
Aim To explore the altered spontaneous cerebral activity patterns and impaired functional regions in patients with diabetic retinopathy (DR) using resting-state functional magnetic resonance imaging ...(rs-fMRI) based on the amplitude of low-frequency fluctuations (ALFF) algorithm. Materials and methods Twenty-one patients with DR (mean age, 54.9±9.9 years; 11 females) and 17 healthy control subjects (54.8±5.7 years; 9 females) were prospectively studied. The DR patients underwent laboratory tests. All individuals underwent a neuropsychological test. The differences in the ALFF values between the two groups were compared. The relationships between ALFF values and clinical measurements were analysed using a multiple-factor analysis. Results Compared to the controls, the DR group showed significantly increased ALFF values in the bilateral occipital gyrus, right lingual gyrus, and precuneus, and decreased values in the right posterior/anterior cerebellar lobe and the parahippocampal, fusiform, superior temporal, inferior parietal, and angular gyrus. Furthermore, the Montreal Cognitive Assessment (MoCA) scores were negatively correlated with decreased ALFF values in the right occipital lobe of the DR group, while increased ALFF values in the right precuneus and lingual gyrus were found to be positively correlated with glycosylated haemoglobin (HbA1c) levels. Conclusions Patients with DR showed spontaneous cerebral activity abnormalities in many cerebral regions that were associated with cognitive impairments. HbA1c levels altered spontaneous cerebral activity in DR patients.
STUDY QUESTION
Is preimplantation genetic diagnosis (PGD) for translocation carriers more effective when done with a single-nucleotide polymorphism (SNP) array using trophectoderm (TE) biopsy and ...frozen embryo transfer (FET) compared with traditional PGD based on fluorescence in situ hybridization (FISH-PGD) using blastomere biopsy and fresh embryo transfer?
SUMMARY ANSWER
The procedure using the SNP array combined with TE biopsy and FET significantly improves the clinical pregnancy rate for translocation carriers. The miscarriage rate also slightly decreases.
WHAT IS KNOWN ALREADY
FISH-PGD has been widely used in translocation carriers but the clinical outcomes have not been ideal. SNP arrays can detect both chromosome segmental imbalances and aneuploidy, and may overcome the limitations of FISH in PGD for translocation carriers.
STUDY DESIGN, SIZE AND DURATION
This was a retrospective study of 575 couples with chromosomal translocations, including 169 couples treated by SNP-PGD between October 2011 and August 2012, and 406 couples treated by FISH-PGD between January 2005 and October 2011.
PARTICIPANTS/MATERIALS, SETTING, METHODS
The study was set in an IVF center at the Reproductive and Genetic Hospital of CITIC-Xiangya, China. In total, 169 couples underwent SNP analysis, including 52 Robertsonian translocation carriers and 117 carriers of reciprocal translocations. Blastocysts (n = 773) were biopsied and FET was carried out on the balanced embryos. Four hundred and six couples underwent FISH-PGD, including 149 Robertsonian translocation carriers and 257 reciprocal translocation carriers. In total, 3968 embryos were biopsied and balanced embryos were transferred fresh. The SNP-PGD results and clinical outcomes were compared with those of FISH-PGD.
MAIN RESULTS AND THE ROLE OF CHANCE
Reliable SNP-PGD results were obtained for 717 out of 773 (92.8%) biopsied blastocysts. The proportions of normal/balanced embryos, embryos with translocation-related and translocation-unrelated abnormalities, the median number of embryos per patient, the ongoing pregnancy rate per embryo transfer and the miscarriage rate were 58, 23, 19, 2, 69 and 12%, respectively, for Robertsonian translocation carriers and 36, 52, 12, 1, 74 and 11%, respectively, in reciprocal translocation carriers. Reliable FISH-PGD results were obtained for 3452 out of 3968 (87.0%) biopsied embryos. The proportions of normal/balanced embryos, unbalanced embryos, the median number of embryos per patient, the ongoing pregnancy rate per transfer and the miscarriage were 36, 64, 3, 38 and 17%, respectively, for Robertsonian translocation carriers and 20, 80, 1, 39 and 16%, respectively, for reciprocal translocation carriers. Thus, SNP-PGD achieved a higher pregnancy rate but a lower miscarriage rate than FISH-PGD. There were no significant differences in maternal age, basal endocrine level and the average number of retrieved oocytes and good-quality D3 embryos in the SNP-PGD group compared with the FISH-PGD group.
LIMITATIONS, REASONS FOR CAUTION
This was a retrospective study with the two groups treated in different periods; therefore, there is a chance of sample bias and a possibility that the results were influenced by other factors that changed over time. Furthermore, the two treatment protocols differ in several respects and we cannot say which makes the greatest contribution to the difference in success. Complete pregnancy outcomes of SNP-PGD have not been obtained as some embryos have not been transferred yet. We cannot exclude differences between the final data and the data in the present manuscript.
WIDER IMPLICATIONS OF THE FINDINGS
The adoption of SNP-PGD combined with TE biopsy and FET may significantly improve the clinical pregnancy rate, and decrease the miscarriage rate after PGD for translocation carriers.
STUDY FUNDING/COMPETING INTEREST(S)
This work was supported by a grant from the Major State Basic Research Development Program of China (No. 2012CB944901) and National Science Foundation of China (No. 81222007). The authors have no competing interests to declare.
TRIAL REGISTRATION NUMBER
Not applicable.
Iron selenide superconductors exhibit a number of unique characteristics that are helpful for understanding the mechanism of superconductivity in high-Tc iron-based superconductors more generally. ...However, in the case of AxFe2Se2 (A = K, Rb, Cs), the presence of an intergrown antiferromagnetic insulating phase makes the study of the underlying physics problematic. Moreover, FeSe-based systems intercalated with alkali metal ions, NH3 molecules or organic molecules are extremely sensitive to air, which prevents the further investigation of their physical properties. It is therefore desirable to find a stable and easily accessible FeSe-based superconductor to study its physical properties in detail. Here, we report the synthesis of an air-stable material, (Li0.8Fe0.2)OHFeSe, which remains superconducting at temperatures up to ~40 K, by means of a novel hydrothermal method. The crystal structure is unambiguously determined by a combination of X-ray and neutron powder diffraction and nuclear magnetic resonance. Moreover, antiferromagnetic order is shown to coexist with superconductivity. This synthetic route opens a path for exploring superconductivity in other related systems, and confirms the appeal of iron selenides as a platform for understanding superconductivity in iron pnictides more broadly.
Faced with unresolved tensions between neutrino interaction measurements at few-GeV neutrino energies, current experiments are forced to accept large systematic uncertainties to cover discrepancies ...between their data and model predictions. The widely used pion production model in GENIE is compared to four MINERνA charged current pion production measurements using nuisance. Tunings, i.e., adjustments of model parameters, to help match GENIE to MINERνA and older bubble chamber data are presented. We find that scattering off nuclear targets as measured in MINERνA is not in good agreement with expectations based upon scattering off nucleon (hydrogen or deuterium) targets in existing bubble chamber data. An additional ad hoc correction for the low−Q2 region, where collective nuclear effects are expected to be large, is presented. While these tunings and corrections improve the agreement of GENIE with the data, the modeling is imperfect. The development of these tunings within the nuisance framework allows for straightforward extensions to other neutrino event generators and models, and allows omitting and including new datasets as they become available.
An 8‐week feeding trial was performed to investigate effects of dietary selenium (sodium selenite, Na2SeO3; selenium nanoparticle, Nano‐Se; selenium yeast, Se‐yeast) on the growth, selenium status, ...antioxidant activities, muscle composition and meat quality of blunt snout bream. Na2SeO3 and Nano‐Se were supplemented at 0.2 mg Se kg−1, and Se‐yeast was supplemented at 0.1, 0.2, 0.4 and 0.8 mg Se kg−1 in basal diet; no Se was added as a control. The results indicated that groups of 0.2 and 0.4 mg kg−1 Se‐yeast had significantly higher weight gain and Nano‐Se, 0.2, 0.4 mg kg−1 Se‐yeast had significantly lower feed conversation ratio compared with the control group. The Se concentrations of whole body, muscle and liver linearly increased with increasing dietary Se‐yeast levels. Group of 0.4 mg kg−1 Se‐yeast significantly increased activities of catalase and glutathione peroxidase. Muscle colour of Nano‐Se, 0.2, 0.4 mg kg−1 Se‐yeast groups, the water‐holding capacity of 0.4, 0.8 mg kg−1 Se‐yeast groups showed significant differences compared with the control group. The results suggest that Se‐yeast and Nano‐Se had a better growth performance than Na2SeO3 at 0.2 mg Se kg−1, and supplementing appropriate Se‐yeast in diet can increase antioxidant activities and enhance meat quality of blunt snout bream.
Primary ovarian insufficiency (POI) is the depletion or loss of normal ovarian function, which cause infertility in women before the age of 40 years. Two homozygous germline truncation mutations in ...STAG3 gene had been reported to causes POI in consanguineous families. Here, we aimed to identify the genetic cause of POI in 2 affected sisters manifested with primary amenorrhea and partial development of secondary sexual characters with normal range of height of a consanguineous Han Chinese family. Whole‐exome and Sanger sequencing identified a homozygous donor splice‐site mutation (NM_012447.2: c.1573+5G>A) in the STAG3 gene. RT‐PCR revealed that the mutation causes loss of wild‐type donor splice‐site which leads to aberrant splicing of STAG3 mRNA and consecutive formation of STAG3 alternative transcript (p.Leu490Thrfs*10) . This is the first report of splice‐site mutation of STAG3 gene causes POI in 2 Han Chinese patients.
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