•We investigate the interfacial behavior of hydrophilic and/or charged NPs via a partially miscible fluid-based droplet microfluidic system.•The movement of droplets accelerates their dissolution, ...leading to the close packing of the NPs at the liquid–liquid interface and the formation of colloidosomes.•The size of the generated colloidosomes can be adjusted on demand by tuning the concentration of the charged AuNPs and droplet size.•The synthesized colloidosomes can be used as a signal-enhanced probe for bacterial infection in lateral flow assay, and the detection limit is 0.025 μg/mL.•The generated colloidosomes can encapsulate cargo molecules with 99.76% efficiency and release these molecules at a rate of 0.096% every five days.
Colloidosomes are spherical shells composed of closed-packed nanoparticles, which can be prepared through the self-assembly of nanoparticles at the interphase of two immiscible liquids. However, the method leads to colloidosomes of various sizes and limited synthesis due to charge repulsion and nanoparticle wettability. Herein, a microfluidic droplet device and partially miscible liquids are integrated for colloidosome synthesis. Dissolving liquids within droplets enables hydrophilic nanoparticles to close packing at the liquid interface to form colloidosomes. Moreover, the microfluidic droplet system achieved colloidosomes within a narrow and specific range of sizes. The close-packing of nanoparticles is proved by modifying colloidosomes into probes in signal-enhanced lateral flow assays (LFAs). Due to the plasmon resonance effect, the testing lines are black, demonstrating the close packing of nanoparticles and their signal enhancement property compared to traditional LFAs. Accordingly, this approach is a potential bacterial detection platform with a detection time of 10 min, higher sensitivity, and specificity. Moreover, since colloidosomes are hollow spheres, they are capable of drug cargo with an encapsulation rate of 99.76 %. With this proposed partially miscible fluid-based droplet microfluidic system, colloidosomes can be fabricated with versatile properties, extending their application to advanced material fabrication, drug encapsulation, and biosensing.
The introduction of service robots in the public domain has introduced a paradigm shift in how robots are interacting with people, where robots must learn to autonomously interact with the untrained ...public instead of being directed by trained personnel. As an example, a hospital service robot is told to deliver medicine to Patient Two in Ward Three. Without awareness of what "Patient Two" or "Ward Three" is, a service robot must systematically explore the environment to perform this task, which requires a long time. The implementation of a Semantic Map allows for robots to perceive the environment similar to people by associating semantic information with spatial information found in geometric maps. Currently, many semantic mapping works provide insufficient or incorrect semantic-metric information to allow a service robot to function dynamically in human-centric environments. This paper proposes a semantic map with a hierarchical semantic organization structure based on a hybrid metric-topological map leveraging convolutional neural networks and spatial room segmentation methods. Our results are validated using multiple simulated and real environments on our lab's custom developed mobile service robot and demonstrate an application of semantic maps by providing only vocal commands. We show that this proposed method provides better capabilities in terms of semantic map labeling and retain multiple levels of semantic information.
Streptococcus pneumoniae (pneumococcus) is the most common respiratory pathogen worldwide. Nasopharyngeal carriage with S. pneumoniae is the major source of lower respiratory tract infection and ...horizontal spread among children. Investigating nasopharyngeal S. pneumoniae is crucial for clinicians to control pneumococcus disease. Here, we retrospectively analyzed clinical information of 5,960 hospitalized children, focusing on pneumonia children less than five years with positive nasopharyngeal pneumococcal cultures. Nasopharyngeal aspirates (NPAs) were collected between June 2009 and December 2016, which were outside the pneumococcal conjugate vaccine(PCV) period. NPAs were subjected to common bacterial culture and antibiotic susceptibility tests, and serotypes were identified by both multiplex PCR and DNA sequencing. Results clearly revealed that clinical manifestations of the children whose NPAs were S. pneumoniae culture positive were serious, especially in those less than twelve months old. Fifteen different serotypes of nasopharyngeal S. pneumoniae were detected, the most common ones being 19F (35.2%), 6A/B (23.8%), 19A (11.4%), 15B/C (9.3%) and 23F (7.8%). Eight serotypes, accounting for 85.5% of the isolates, corresponded to the PCV13 serotypes. Approximately one-third of all S. pneumoniae strains were susceptible to penicillin. Overall, we consider nasopharyngeal S. pneumoniae culture is beneficial in assessing the situations of pneumonia children. Moreover, PCV13 could be useful in preventing pneumococcal disease in Chongqing, China.
Introduction
Human adenovirus 7 (HAdV‐7) is an important viral pathogen of severe pneumonia in children and a serious threat to health.
Methods
A cohort of 45 pediatric patients diagnosed with ...HAdV‐7‐associated severe pneumonia and admitted to the Pediatric Intensive Care Unit at the Children's Hospital of Chongqing Medical University from May 2018 to January 2020 were included. Risk factors of death were analyzed by the Cox proportional risk mode with Clinical data, serum, and nasopharyngeal aspirate adenovirus load, Genome analysis, Olink proteomics, and cytokine profile between dead and surviving patients were also analyzed.
Results
A total of 45 children with a median age of 12.0 months (interquartile range IQR: 6.5, 22.0) were included (female 14), including 14 (31.1%) who died. High serum viral load was an independent risk factor for mortality (hazard ratio HR = 2.16, 95% confidence interval CI, 1.04–4.49, p = 0.039). BTB and CNC homology 1 (BACH1), interleukin‐5 (IL‐5), and IL‐9 levels were significantly correlated with serum viral load (p = 0.0400, 0.0499, and 0.0290; r = 0.4663, 0.3339, and −0.3700, respectively), with significant differences between the dead and survival groups (p = 0.021, 0.001, and 0.021).
Conclusions
Severe cytokine storm‐associated high serum viral load after HAdV‐7 infection may be the main mechanism responsible for poor prognosis in children.
Endoscopy is the primary modality for detecting asymptomatic esophageal squamous cell carcinoma (ESCC) and precancerous lesions. Improving detection rate remains challenging. We developed a system ...based on deep convolutional neural networks (CNNs) for detecting esophageal cancer and precancerous lesions high-risk esophageal lesions (HrELs) and validated its efficacy in improving HrEL detection rate in clinical practice (trial registration ChiCTR2100044126 at www.chictr.org.cn). Between April 2021 and March 2022, 3117 patients ≥50 years old were consecutively recruited from Taizhou Hospital, Zhejiang Province, and randomly assigned 1:1 to an experimental group (CNN-assisted endoscopy) or a control group (unassisted endoscopy) based on block randomization. The primary endpoint was the HrEL detection rate. In the intention-to-treat population, the HrEL detection rate 28 of 1556 (1.8%) was significantly higher in the experimental group than in the control group 14 of 1561 (0.9%),
= 0.029, and the experimental group detection rate was twice that of the control group. Similar findings were observed between the experimental and control groups 28 of 1524 (1.9%) versus 13 of 1534 (0.9%), respectively;
= 0.021. The system's sensitivity, specificity, and accuracy for detecting HrELs were 89.7, 98.5, and 98.2%, respectively. No adverse events occurred. The proposed system thus improved HrEL detection rate during endoscopy and was safe. Deep learning assistance may enhance early diagnosis and treatment of esophageal cancer and may become a useful tool for esophageal cancer screening.
RNA live-cell imaging systems have been used to visualize subcellular mRNA distribution in living cells. The RNA-binding protein (RBP)-based RNA imaging system exploits specific RBP and the ...corresponding RNA recognition sequences to indirectly label mRNAs. Co-expression of fluorescent protein-fused RBP and target mRNA conjugated with corresponding RNA recognition sequences allows for visualizing mRNAs by confocal microscopy. To minimize the background fluorescence in the cytosol, the nuclear localization sequence has been used to sequester the RBP not bound to mRNA in the nucleus. However, strong fluorescence in the nucleus may limit the visualization of nucleus-localized RNA and sometimes may interfere in detecting fluorescence signals in the cytosol, especially in cells with low signal-to-noise ratio.
We eliminated the background fluorescence in the nucleus by using the split fluorescent protein-based approach. We fused two different RBPs with the N- or C-terminus of split fluorescent proteins (FPs). Co-expression of RBPs with the target mRNA conjugated with the corresponding RNA recognition sequences can bring split FPs together to reconstitute functional FPs for visualizing target mRNAs. We optimized the system with minimal background fluorescence and used the imaging system to visualize mRNAs in living plant cells.
We established a background-free RNA live-cell imaging system that provides a platform to visualize subcellular mRNA distribution in living plant cells.
Our previous study showed that neonatal S. pneumoniae infection aggravated airway inflammation and airway hyperresponsiveness (AHR) in an OVA-induced allergic asthma model. As airway smooth muscle ...(ASM) plays a pivotal role in AHR development, we aim to investigate the effects of neonatal S. pneumoniae pneumonia on ASM structure and AHR development. Non-lethal neonatal pneumonia was established by intranasally infecting 1-week-old BALB/C mice with the S. pneumoniae strain D39. Five weeks after infection, the lungs were collected to assess the levels of α-SMA and the contractile proteins of ASM. Our results indicate that neonatal S. pneumoniae pneumonia significantly increased adulthood lung α-SMA and SMMHC proteins production and aggravated airway inflammatory cells infiltration and cytokines release. In addition, the neonatal S. pneumoniae pneumonia group had significantly higher Penh values compared to the uninfected controls. These data suggest that neonatal S. pneumoniae pneumonia promoted an aberrant ASM phenotype and AHR development in mice model.
The UK screening and treatment of retinopathy of prematurity (ROP) updated 2022 guidelines were developed by a multidisciplinary guideline development group from the Royal College of Paediatrics and ...Child Health and the Royal College of Ophthalmologists, following the standards of the National Institute for Health and Care Excellence. They were published on the websites of the Royal College of Paediatrics and Child Health and the Royal College of Ophthalmologists in March 2022, and formally published in
in March 2023. The guidelines provide evidence-based recommendations for the screening and treatment of ROP. The most significant change in the 2022 updated version compared to the previous guidelines is the lowering of the gestational age screening criterion to below 31 weeks. The treatment section covers treatment indications, timing, methods, and follow-up visits of ROP. This article interprets the guidelines and compares them with ROP guidelines/consensus in China, providing a reference for domestic peers.
This study aimed to investigate whether self-rated health status (SRH) and subjective health complaints (SHC) of urban Chinese women are associated with their health-promoting lifestyles (HPL).
We ...conducted a cross-sectional study on 8142 eligible Chinese participants between 2012 and 2013. Demographic and SHC data were collected. Each subject completed the SRH questionnaire and the Chinese version of the Health-Promoting Lifestyle Profile-II (HPLP-II). Correlation and binary regression analyses were performed to examine the associations of SRH and SHC with HPL.
Both SRH and HPL of urban Chinese women were moderate. The most common complaints were fatigue (1972, 24.2%), eye discomfort (1571, 19.3%), and insomnia (1542, 18.9%). Teachers, highly educated subjects and elderly women had lower SRH scores, while college students and married women had better HPL. All items of HPLP-II were positively correlated with SRH (r = 0.127-0.533, P = 0.000) and negatively correlated with SHC to a significant extent (odds ratio OR = 1.40-11.37).
Aspects of HPL, particularly stress management and spiritual growth, are associated with higher SRH and lower SHC ratings among urban Chinese women. Physical activity and health responsibility are additionally related to reduced fatigue and nervousness. We believe that these findings will be instrumental in encouraging researchers and urban women to adopt better health-promoting lifestyles with different priorities in their daily lives.
USPs are a family of enzymes that regulate protein degradation, and their dysregulation has been implicated in the development and progression of cancer.
This study aimed to determine whether ...ubiquitin-specific proteases 3 (USP3) could be a potential target for therapy in hepatocellular carcinoma (HCC), particularly in resistant HCC. This study systematically investigated the role of USP3 in HCC, with a focus on chemo-resistant HCC cells.
The level of USP3 from clinical samples was measured using an ELISA assay. Cell proliferation, apoptosis, migration, and anchorage-independent colony formation assays were performed. Transfection was performed to knock down USP3 expression and measure β-catenin activity, and real-time PCR was used to measure levels of MYC and CYCLIN D1 genes.
USP3 protein was upregulated in HCC tissues, but its upregulation was not associated with clinicopathology. USP3 knockdown had a similar inhibitory effect on growth in both sensitive and resistant HCC cells, did not affect migration, and induced apoptosis in sensitive but not resistant HCC cells. Furthermore, USP3 knockdown was more effective in suppressing anchorage-independent colony formation in chemoresistant HCC cells compared to their chemo-sensitive counterparts. Pearson correlation coefficient analysis revealed a strong positive correlation between USP3 and CTNNB1, and consistently, USP3 knockdown reduced the levels and activities of β-catenin in HCC cells. Using a Wnt activator (lithium) in rescue studies significantly reversed the inhibitory effects of USP3 knockdown.
The findings suggest that inhibiting USP3 is an effective strategy against cancer stem cells and chemo-resistant HCC cells.
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