Short chain fatty acids (SCFAs) are the main products of indigestible carbohydrates that are fermented by microbiota in the hindgut. This study was designed to investigate the effects of oral SCFAs ...administration on the lipid metabolism of weaned pigs. A total of 21 barrows were randomly allocated into three groups, including control group (orally infused with 200 mL physiological saline per day), low dose SCFAs group (orally infused with 200 mL SCFAs containing acetic acid 20.04 mM, propionic acid 7.71 mM and butyric acid 4.89 mM per day), and high dose SCFAs group (orally infused with 200 mL SCFAs containing acetic acid 40.08 mM, propionic acid 15.42 mM and butyric acid 9.78 mM per day). The results showed that the average daily feed intake of SCFAs groups were lower than that of control group (P<0.05). Oral administration of SCFAs decreased the concentrations of triglyceride (TG), total cholesterol (TC), high density lipoprotein-cholesterol and insulin (P<0.05), and increased the leptin concentration in serum (P<0.05). The total fat, as well as TC and TG levels in liver, was decreased by oral SCFAs administration (P<0.05). In addition, SCFAs down-regulated the mRNA expressions of fatty acid synthase (FAS) and sterol regulatory element binding protein 1c (P<0.05), and enhanced the mRNA expression of carnitine palmitoyltransferase-1α (CPT-1α) in liver (P<0.05). SCFAs also decreased FAS, acetyl-CoA carboxylase (ACC) and peroxisome proliferator activated receptor σ mRNA expressions in longissimus dorsi (P<0.05). And in abdominal fat, SCFAs reduced FAS and ACC mRNA expressions (P<0.05), and increased CPT-1α mRNA expression (P<0.05). These results suggested that oral administration of SCFAs could attenuate fat deposition in weaned pigs via reducing lipogenesis and enhancing lipolysis of different tissues.
Abstract Previous data demonstrate that traumatic brain injury (TBI) activates autophagy, and increases microtubule-associated protein 1 light chain 3 (LC3) immunostaining mainly in neurons. However, ...the role of autophagy in traumatic brain damage remains elusive. The aim of the present study was to investigate the autophagic mechanisms participating in traumatic brain injury. The autophagy inhibitors 3-methyladenine (3-MA) and bafliomycin A1 (BFA) were administered with a single i.c.v. injection before TBI. We first examined the protein levels of Beclin-1 and LC3 II, which have been found to promote autophagy previously. Immunoblotting analysis showed that 3-MA pretreatment reduced post-TBI Beclin-1 and LC3-II levels, and maintained p62/SQSTM1 (p62) levels. In addition, double immunolabeling showed that the increased punctate LC3-II dots colocalizing with Propidium Iodide (PI)-stained nuclei at 24 h after injury, were partially inhibited by 3-MA pretreatment. Furthermore, inhibition of autophagy could reduce TBI-induced cell injury assessed with i.p. injection of PI and lesion volume, and attenuate behavioral outcome evaluated by motor test and Morris water maze. The neuroprotective effects were associated with an inhibition on TBI-induced up-regulation of LC3, Beclin-1, cathepsin B, caspase-3 and the Beclin-1/Bcl-2 ratio. Taken together, these data imply that the autophagy pathway is involved in the pathophysiologic responses after TBI, and inhibition of this pathway may help attenuate traumatic damage and functional outcome deficits.
Deformation twinning in pure aluminum has been considered to be a unique property of nanostructured aluminum. A lingering mystery is whether deformation twinning occurs in coarse-grained or ...single-crystal aluminum at scales beyond nanotwins. Here, we present the first experimental demonstration of macrodeformation twins in single-crystal aluminum formed under an ultrahigh strain rate (∼10^{6} s^{-1}) and large shear strain (200%) via dynamic equal channel angular pressing. Large-scale molecular dynamics simulations suggest that the frustration of subsonic dislocation motion leads to transonic deformation twinning. Deformation twinning is rooted in the rate dependences of dislocation motion and twinning, which are coupled, complementary processes during severe plastic deformation under ultrahigh strain rates.
Here, in an analysis of a 2.92 fb–1 data sample taken at 3.773 GeV with the BESIII detector operated at the BEPCII collider, we measure the absolute decay branching fractions to be B(D0 → K–e+νe) = ...(3.505 ± 0.014 ± 0.033)% and B(D0 → π–e+νe) = (0.295 ± 0.004 ± 0.003)%. From a study of the differential decay rates we obtain the products of hadronic form factor and the magnitude of the CKM matrix element $f$ $^{K}_{+}$(0)|Vcs| = 0.7172 ± 0.0025 ± 0.0035 and $f$ $^{π}_{+}$(0)|Vcd| = 0.1435 ± 0.0018 ± 0.0009.
The cross sections of e+e-→π+π-hc at center-of-mass energies from 3.896 to 4.600 GeV are measured using data samples collected with the BESIII detector operating at the Beijing Electron Positron ...Collider. The cross sections are found to be of the same order of magnitude as those of e+e-→π+π- J/ψ and e+e-→π+π-ψ (2S), but the line shape is inconsistent with the Y states observed in the latter two modes. Two structures are observed in the e+e- → π+π- hc cross sections around 4.22 and 4.39 GeV / c 2 , which we call Y ( 4220 ) and Y ( 4390 ) , respectively. A fit with a coherent sum of two Breit-Wigner functions results in a mass of (4218.4 $+5.5\atop{-4.5 ± 0.9) MeV/c2 and a width of 66.0$+12.3\atop-8.3$±0.4 MeV for the Y (4220), and a mass of (4391.5 $+6.3\atop-16.8$ ± 1.0) MeV/c2 and a width of (139.5$+16.2\atop-20.6 ± 0.6) MeV for the Y (4390), where the first uncertainties are statistical and the second ones systematic. The statistical significance of Y ( 4220 ) and Y(4390) is 10σ over one structure assumption.
The nodal-line semimetals have attracted immense interest due to the unique electronic structures such as the linear dispersion and the vanishing density of states as the Fermi energy approaching the ...nodes. Here, we report temperature-dependent transport and scanning tunneling microscopy (spectroscopy) STM(S) measurements on nodal-line semimetal ZrSiSe. Our experimental results and theoretical analyses consistently demonstrate that the temperature induces Lifshitz transitions at 80 and 106 K in ZrSiSe, which results in the transport anomalies at the same temperatures. More strikingly, we observe a V-shaped dip structure around Fermi energy from the STS spectrum at low temperature, which can be attributed to co-effect of the spin-orbit coupling and excitonic instability. Our observations indicate the correlation interaction may play an important role in ZrSiSe, which owns the quasi-two-dimensional electronic structures.
Fatigue life scattering and prediction of Inconel 718 fabricated by selective laser melting were investigated using miniature specimen tests combined with statistical method and machine learning ...algorithms. The relationship between pore features and fatigue life of the selective laser melting-fabricated specimens was analyzed statistically. The results show that the increase in the size and/or the number of the pores in the specimens, and/or the decrease in the distance from a pore center to the specimen surface degraded the fatigue life. The machine learning and statistical analysis results reveal that the fatigue life are most closely related to the location of the pores compared with the size and the number of pores in the specimens. The finding may provide a potential way to get high-throughput statistical data helping in evaluating defect-dominated scattering and prediction of fatigue life of additive manufactured metallic parts using miniature specimen testing assisted by the machine learning approach.
In this paper, the spin and parity of the Zc(3900)± state are determined to be JP = 1+ with a statistical significance larger than 7σ over other quantum numbers in a partial wave analysis of the ...process e+e- → π+π-J/Ψ. We use a data sample of 1.92 fb-1 accumulated at $ \sqrt{s}=4.23 $ and 4.26 GeV with the BESIII experiment. When parametrizing the Zc(3900)± with a Flatté-like formula, we determine its pole mass Mpole = (3881.2±4.2stat ±52.7syst) MeV/c2 and pole width Γpole = (51.8± 4.6stat ± 36.0syst) MeV. Finally, we also measure cross sections for the process e+e- → Zc(3900)+π- + c.c. → J/Ψπ+π- and determine an upper limit at the 90% confidence level for the process e+e- → Zc(4020)+π- + c.c. → J/Ψ π+π-.
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