•Autonomous underground mining technology transfer to large diameter pipes and tunnels.•Onboard inertial positioning system with profiling, 3D referencing and gyro/laser surveying.•Real time creation ...of 3D maps to pinpointing underground infrastructure problems.
This paper presents technology applications from the autonomous mining and construction industries in tunnel and underground environments as applied to critical large diameter utility infrastructure. A self-contained inertial navigation system for the positioning and mapping of underground infrastructures is a significant development in tunnel profiling, 3D referencing and gyro/laser surveying; a key service offered by the results of this project. The underground positioning relies on a network of satellites placed to surround an area of interest, with a range of up to 2km through soil or rock with accuracy better than 3%, enabling accurate positioning of underground assets. The robotic mapping system has the capabilities to accurately map tunnels, pipes and conduits, in detail and sequentially transfer the data collected into popular engineering CAD systems. A specialized military grade inertial referencing system (IRS) linked to multiple scanners provides high precision profiling while measuring roughness, deflection, ovality and positioning. The IRS component is linked to multiple laser scanners supplying high precision profiling while being driven forward. Laser scanning collects hundreds of data points per second linked to an accurate position through the IRS. All data is collected to on-board computer hard drives and transferred to the engineering office via memory storage systems or directly by wireless networks set up within the pipeline. Combining sectional scans with positioning and altitude data in real time creates 3D maps for surface referencing, a valuable service for pinpointing underground infrastructure problem locations in relation to surface features enabling informed risk management decisions.
The bioaccumulation of trace elements in aquatic organisms can be described with a kinetic model that includes linear expressions for uptake and elimination from dissolved and dietary sources. Within ...this model, trace element trophic transfer is described by four parameters: the weight-specific ingestion rate (IR); the assimilation efficiency (AE); the physiological loss rate constant (
k
e
); and the weight-specific growth rate (
g). These four parameters define the trace element trophic transfer potential (TTP=IR·AE/
k
e
+
g) which is equal to the ratio of the steady-state trace element concentration in a consumer due to trophic accumulation to that in its prey. Recent work devoted to the quantification of AE and
k
e
for a variety of trace elements in aquatic invertebrates has provided the data needed for comparative studies of trace element trophic transfer among different species and trophic levels and, in at least one group of aquatic consumers (marine bivalves), sensitivity analyses and field tests of kinetic bioaccumulation models. Analysis of the trophic transfer potentials of trace elements for which data are available in zooplankton, bivalves, and fish, suggests that slight variations in assimilation efficiency or elimination rate constant may determine whether or not some trace elements (Cd, Se, and Zn) are biomagnified. A linear, single-compartment model may not be appropriate for fish which, unlike many aquatic invertebrates, have a large mass of tissue in which the concentrations of most trace elements are subject to feedback regulation.
Diet is an important exposure route for the uptake of trace metals by aquatic invertebrates, with trace metal trophic transfer depending on 2 stages - assimilation and subsequent accumulation by the ...predator. This study investigated the trophic transfer of trace metals from the sediment-dwelling polychaete worm Nereis diversicolor from metal-rich estuarine sediments in southwestern UK to 2 predators - another polychaete N. virens (Cu, Zn, Pb, Cd, Fe) and the decapod crustacean Palaemonetes varians (Cu, Zn, Pb, Cd, Fe, Ag, As, Mn). N. virens showed net accumulation of Cu, Zn, Pb and Cd from the prey; accumulation increased with increasing prey concentration, but a coefficient of trophic transfer decreased with increasing prey concentration, probably because a higher proportion of accumulated metal in the prey is bound in less trophically available (insoluble) detoxified forms. The trace metal accumulation patterns of P. varians apparently restricted significant net accumulation of metals from the diet of N. diversicolor to just Cd. There was significant mortality of the decapods fed on the diets of metal-rich worms. Metal-rich invertebrates that have accumulated metals from the rich historical store in the sediments of particular SW England estuaries can potentially pass these metals along food chains, with accumulation and total food chain transfer depending on the metal assimilation efficiencies and accumulation patterns of the animal at each trophic level. This trophic transfer may be significant enough to have ecotoxicological effects.
Biodynamic modelling has been used to predict bioaccumulated concentrations of Ag, Cd and Zn in the deposit-feeding polychaete Nereis diversicolor from 5 metal-contaminated estuaries in SW England ...and a relatively non-contaminated estuary in SE England. The modelling employed previously measured physiological parameters of bioaccumulation - uptake rate constant, assimilation efficiency (AE) and efflux rate constants after uptake from water and sediment ingestion - and measured sediment metal concentrations specific for each population. AEs were considered to relate to metals in the organic component of the ingested sediment and ingestion rates were therefore expressed in these terms, with the further assumption that the total sediment metal concentration is a proxy for the metal concentration in the sediment organic component. A range of growth rate constants was extracted from the literature, as were concentration ranges of dissolved Ag, Cd and Zn in contaminated coastal waters. The model showed that >99% Cd and >98% Zn accumulated by N. diversicolor is derived from sediment ingestion; more bioaccumulated Ag is derived from solution, the percentage contribution of the dissolved source increasing from 46 to 80% with an increase in Ag dissolved concentration from low to high values for coastal waters. Bioaccumulated metal concentrations predicted from the model generally showed excellent agreement with independently measured concentrations in field-collected worms, supporting the assumptions made in the model.
Trophic transfer of trace metals Rainbow, P. S.; Poirier, L.; Smith, B. D. ...
Marine ecology. Progress series,
02/2006, Letnik:
308
Journal Article
Recenzirano
Odprti dostop
The chemical form of accumulated trace metal in prey is important in controlling the bioavailability of dietary metal to a predator. This study investigated the trophic transfer of radiolabelled Ag, ...Cd and Zn from the polychaete wormNereis diversicolorto the decapod crustaceanPalaemonetes varians. We used 2 populations of worms with different proportions of accumulated metals in different subcellular fractions as prey, and loaded the worms with radiolabelled metals either from sediment or from solution. Accumulated radiolabelled metals were fractionated into 5 components:metal-rich granules (MRG), cellular debris, organelles, metallothionein-like proteins (MTLP), and other (heat-sensitive) proteins (HSP). Assimilation efficiencies (AE) of the metals byP. varianswere measured from the 4 categories of prey (i.e. 2 populations, radiolabelled from sediment or solution). There were significant differences for each metal between the AEs from the different prey categories, confirming that origin of prey and route of uptake of accumulated trace metal will cause intraspecific differences in subsequent metal assimilation. Correlations were sought between AEs and selected fractions or combinations of fractions of metals in the prey-MRG, Trophically Available Metal (TAM = MTLP + HSP + organelles) and total protein (MTLP + HSP). TAM explained 28% of the variance in AEs for Ag, but no consistent relationships emerged between AEs and TAM or total protein when the metals were considered separately. AEs did, however, show significant positive regressions with both TAM and total protein when the 3 metals were considered together, explaining only about 21% of the variance in each case. A significant negative relationship was observed between MRG and AE for all metals combined. The predator (P. varians) can assimilate dietary metal from a range of the fractions binding metals in the prey (N. diversicolor), with different assimilation efficiencies summated across these fractions. TAM and/or total protein may represent an approximate minimum for trophic availability but neither of these alone is a fully accurate predictor.
Oxidative processes play an important role in atherogenesis. Because superoxide anion and nitric oxide (NO) are important mediators in vascular pathology, we studied the expression of extracellular ...superoxide dismutase (EC-SOD) and inducible nitric oxide synthase (iNOS) in human and rabbit atherosclerotic lesions by using simultaneous in situ hybridization and immunocytochemistry and EC-SOD enzyme activity measurements. We also analyzed the presence in the arterial wall of oxidized lipoproteins and peroxynitrite-modified proteins as indicators of oxidative damage and possible mediators in vascular pathology. EC-SOD and iNOS mRNA and protein were expressed in smooth muscle cells and macrophages in early and advanced lesions. The expression of both enzymes was especially prominent in macrophages. As measured by enzyme activity, EC-SOD was the major SOD isoenzyme in the arterial wall. EC-SOD activity was higher in highly cellular rabbit lesions but lower in advanced, connective tissue-rich human lesions. Despite the abundant expression of EC-SOD, malondialdehyde-lysine and hydroxynonenal-lysine epitopes characteristic of oxidized lipoproteins and nitrotyrosine residues characteristic of peroxynitrite-modified proteins were detected in iNOS-positive, macrophage-rich lesions, thus implying that malondialdehyde, hydroxynonenal, and peroxynitrite are important mediators of oxidative damage. We conclude that EC-SOD, iNOS, and the balance between NO and superoxide anion play important roles in atherogenesis. EC-SOD and iNOS are highly expressed in lesion macrophages. High EC-SOD expression in the arterial wall may be required not only to prevent deleterious effects of superoxide anion but also to preserve NO activity and prevent peroxynitrite formation. Modulation of arterial EC-SOD and iNOS activities could provide means to protect arteries against atherosclerotic vascular disease.
Oxidation of low density lipoproteins (LDL) obviously plays an important role in the pathogenesis of atherosclerosis. The purpose of the study was to determine whether antibodies against oxidized LDL ...are associated with coronary artery disease (CAD). We determined the serum levels of antibodies against copper-oxidized LDL by enzyme-linked immunosorbent assay in 58 patients with angiographically verified CAD and 34 controls without CAD. The mean antibody level, expressed in optical density units, was significantly higher in patients than in controls (0.150+/-0.088 versus 0.094+/-0.054, respectively; P=0.00089). In logistic regression analysis, high antibody level against oxidized LDL was associated significantly with CAD (P=0.0114), independent of age (P=0.00137), gender (P=0.0021), body mass index (P=0.5947), triglyceride concentration (P=0.9813), and total cholesterol-high density lipoprotein (HDL) cholesterol (P=0.0080) group. Similar analysis in nondiabetic subjects (n=79) and in men only (n=75) showed analogous results, with only minor changes in P values. The antibody level against oxidized LDL differed significantly between nonsmokers and smokers in CAD patients (P<0.00197) but not in controls (P=NS). In addition, the antibody level against oxidized LDL differed significantly between nonsmokers and smokers in subjects with low HDL cholesterol (</=0.9 mmol/L) but not in subjects with high HDL cholesterol (>0.9 mmol/L). In conclusion, elevated levels of antibodies against oxidized LDL were associated with CAD. The data suggest that oxidized LDL plays a role in the pathogenesis of atherosclerosis and suggest a protective function for HDL against LDL oxidation.
A series of experiments was performed to evaluate the extent to which Cd, Co, Ag, Se, Cr, and Zn bound to sediments with different geochemical properties could be assimilated by the mussel Mytilus ...edulis and the clam Macoma balthica. Oxidized and reduced radiolabeled sediments were fed to suspension-feeding animals, the depuration patterns of the individuals were followed by γ-spectrometry, and the assimilation efficiencies (AEs) of ingested metals were determined. AEs from geochemically diverse sediments typically varied less than 2-fold and ranged from 1% for Cr to 42% for Zn. Metals were assimilated from anoxic sediment by both animals; Ag, Cd, and Co AEs in M. balthica were 9−16%, 2-fold lower than from oxic sediment, but in M. edulis AEs were about two times greater from anoxic sediment for all metals but Ag. For oxic sediment, Cd and Co AEs in M. edulis decreased 3−4-fold with increased sediment exposure time to the metals with smaller but significant effects also noted for Zn and Se but not Ag. A less pronounced decrease in AE for M. balthica was evident only after 6 months exposure time. Sequential extractions of the oxidized sediments showed a transfer of metals into more resistant sediment components over time, but the rate did not correlate with a decrease in metal AEs. Comparing the two bivalves, TOC concentrations had an inconsistent effect on metal AEs. AEs of metals from bacteria-coated glass beads were slightly higher than from humic acid-coated beads, which were comparable with whole-sediment AEs. There was correspondence of AE with desorption of Ag, Cd, Co, and Se (but not Zn) from sediments into pH 5 seawater, measured to simulate the gut pH of these bivalves. The results imply that metals associated with sulfides and anoxic sediments are bioavailable, that the bioavailability of metals from sediments decreases over exposure time, that organic carbon content generally has a small effect on AEs, and that AEs of sediment-bound metals differ among species.
Although the differential responses of stream taxa to metal exposure have been exploited for bioassessment and monitoring, the mechanisms affecting these responses are not well understood. In this ...study, the subcellular partitioning of metals in operationally defined metal‐sensitive and detoxified fractions were analyzed in five insect taxa. Samples were collected in two separate years along an extensive metal contamination gradient in the Clark Fork River (MT, USA) to determine if interspecific differences in the metal concentrations of metal‐sensitive fractions and detoxified fractions were linked to the differences in distributions of taxa relative to the gradient. Most of the Cd, Cu, and Zn body burdens were internalized and potentially biologically active in all taxa, although all taxa appeared to detoxify metals (e.g., metal bound to cytosolic metal‐binding proteins). Metal concentrations associated with metal‐sensitive fractions were highest in the mayflies Epeorus albertae and Serratella tibialis, which were rare or absent from the most contaminated sites but occurred at less contaminated sites. Relatively low concentrations of Cu were common to the tolerant taxa Hydropsyche spp. and Baetis spp., which were widely distributed and dominant in the most contaminated sections of the river. This suggested that distributions of taxa along the contamination gradient were more closely related to the bioaccumulation of Cu than of other metals. Metal bioaccumulation did not appear to explain the spatial distribution of the caddisfly Arctopsyche grandis, considered to be a bioindicator of metal effects in the river. Thus, in this system the presence/absence of most of these taxa from sites where metal exposure was elevated could be differentiated on the basis of differences in metal bioaccumulation.
This study examined the extent to which dissolved Cd and Zn uptake rates vary in several aquatic insect taxa commonly used as indicators of ecological health. We further attempted to explain the ...mechanisms underlying observed differences. By comparing dissolved Cd and Zn uptake rates in several aquatic insect species, we demonstrated that species vary widely in these processes. Dissolved uptake rates were not related to gross morphological features such as body size or gill sizefeatures that influence water permeability and therefore have ionoregulatory importance. However, finer morphological features, specifically, the relative numbers of ionoregulatory cells (chloride cells), appeared to be related to dissolved metal uptake rates. This observation was supported by Michaelis−Menten type kinetics experiments, which showed that dissolved Cd uptake rates were driven by the numbers of Cd transporters and not by the affinities of those transporters to Cd. Calcium concentrations in exposure media similarly affected Cd and Zn uptake rates in the caddisfly Hydropsyche californica. Dissolved Cd and Zn uptake rates strongly co-varied among species, suggesting that these metals are transported by similar mechanisms.