Bee bread is a mixture of pollen pellets, honey and lactic acid bacteria, and the basis of food in the hive. In the present study, a Moroccan bee bread has been analyzed concerning the palynological ...analysis, nutritional value and chemical composition (free sugars, organic acids, mineral composition, fatty acid, tocopherols and polyphenols); in vitro antioxidant activity and antimicrobial activity screened against pathogenic bacteria and fungi were also evaluated. The studied bee bread was a good source of protein (19.96 ± 0.08 g/100 g), total free sugars (18 ± 1 g/100 g), macroelements and microelements content, revealed high levels of polyunsaturated fatty acids (64.7 ± 0.4%), tocopherols (10.9 ± 0.9 mg/100 g) and natural antioxidants, such as flavonol glycoside derivatives, being isorhamnetin-O-hexosyl-O-rutinoside the major phenolic compound present. The bee bread showed antioxidant activity and effectiveness against all the bacteria and fungi tested. This work contributes to the chemical and bioactive knowledge of this unexplored natural product.
•Bee bread revealed to be a source of nutrients and bioactive compounds.•Presented high levels of carbohydrates, proteins, and minerals.•Kaempferol-3-O-di-rhamnoside was the most abundant phenolic compound.•Revealed antioxidant activity and inhibited all the tested bacterial and fungi strains.
The popularity of fruits vinegar (FsV) has been increased recently as a healthy drink wealthy in bioactive compounds that provide several beneficial properties. This review was designed in the frame ...of valorization of fruits vinegar as a by-product with high value added by providing overall information on its biochemical constituents and beneficial potencies. It contains a cocktail of bioactive ingredients including polyphenolic acids, organic acids, tetramethylperazine, and melanoidins. Acetic acid is the most abundant organic acid and chlorogenic acid is the major phenol in apple vinegar. The administration of fruits vinegar could prevent diabetes, hypercholesterolemia, oxidative stress, cancer, and boost immunity as well as provide a remarkable antioxidant ability. The production techniques influence the quality of vinegar, and consequently, its health benefits.
Oxidative stress plays a role in hyperoxaluria-induced kidney injury and crystallization. Bee pollen is a hive product with a high content of antioxidants. The antioxidant content and protective ...effect of bee pollen extract (BPE) against ethylene glycol (EG) induced crystalluria, and acute kidney injury (AKI) were investigated. The effect of BPE on the EG-induced liver injury and proteinuria was also examined. Ten groups of male Wister rats were treated daily with vehicle, cystone, BPE (100, 250, and 500 mg/kg b.wt.), and group 6-9 treated with EG, EG + BPE (100, 250, and 500 mg/kg b.wt.) and group 10 EG + cystone. The dose of EG was 0.75% v/v, and the dose of cystone was 500 mg/kg b.wt. On day 30, blood and urine samples were collected for analysis. Kidneys were removed for histopathological study. The antioxidant activity of BPE was assessed, and its total phenols and flavonoids were determined. EG significantly increased urine parameters (pH, volume, calcium, phosphorus, uric acid, and protein), blood urea, creatinine, and liver enzymes (P < 0.05). EG decreased creatinine clearance and urine magnesium and caused crystalluria. Treatment with BPE or cystone mitigates EG's effect; BPE was more potent than cystone (P < 0.05). BPE increases urine volume, sodium, and magnesium compared to the control and EG treated groups. BPE reduces proteinuria and prevents AKI, crystalluria, liver injury, and histopathological changes in the kidney tissue caused by EG. BPE might have a protective effect against EG-induced AKI, crystalluria, proteinuria, and stone deposition, most likely by its antioxidant content and activity.
Monofloral honeys (Bupleurum Spinosum) collected from different geographical locations of Moroccan Middle Atlas were evaluated for various potential attributes. For physicochemical parameters, the ...results showed the following values: pH (4.02–4.81); free acidity (12.16–31.85 mEq/kg); lactonic acidity (4.98–14.14 mEq/kg); total acidity (18.37–45.53 mEq/kg); moisture (15.81–19.20%); ash (0.21–0.55); electrical conductivity (427.38–682.16 mS/cm); diastasic activity (10.76–26.01); TSS (80.40–84.20%); melanoidins (0.21–1.15), and Pfund scale (30.47–175.53 mm). Potassium was the most important mineral among the tent evaluated elements, followed by sodium and calcium. All tested samples have high antioxidant activities and correlate with phenols, flavonoids, and ascorbic acid content. In regards to safety (sulfite-reducing clostridia and Salmonella spp.) and sanitary quality (faecal coliforms), all honey samples were negative. In addition, low counts of yeast and molds were detected, and the values of total aerobic mesophilic bacteria and Bacillus Sp. confirm the good quality of honey. The outcome of this study showed that all samples have good physicochemical properties, high antioxidant activities, and acceptable microbiological state, respecting the quality control criteria set by the international norms.
Solanum elaeagnifolium is among the invasive plants of Morocco; studies on its chemical composition and biological activities are few in number in Morocco. S. elaeagnifolium has shown molluscicidal ...and nematicidal and cancer-inhibitory effects, anti-inflammatory, analgesic activity, and antibacterial activity. The objective of this research is to improve this plant and assess its antibacterial and antioxidant properties as well as its total polyphenolic content (TPC) and total flavonoid content (TFC). The Folin-Ciocalteu method and the aluminium-trichloride method were used to determine TPC and TFC in hydro-ethanolic (HEE) and hydro-acetonic (HAE) leaf extract. Three assays were performed to determine the antioxidant activity: the DPPH test (radical 2,2’-diphenyl-1-picrylhydrazyl), the FRAP test (Ferric Reducing Antioxidant Power), and the TAC test. Disk diffusion and microdilution were used to test antibacterial activity against four pathogenic bacteria and Candida albicans. The hydro-ethanolic extract 2.54 ± 0.4 mg EAG/g has a greater polyphenol concentration than the hydro-acetonic extract 1.58 ± 0.03 mg EAG/g. Although the flavonoid content of the hydro-acetonic extract (0.067 ± 0.001 mg EQ/g) is larger than that of the hydro-ethanolic extract (0.012 ± 0.001 mg EQ/g), the flavonoid content of the hydro-ethanolic extract (0.012 ± 0.001 mg EQ/g). The DPPH values were IC-50 = 0.081 ± 0.004 mg/mL for hydro-ethanoic extract and 0.198 ± 0.019 mg/mL for hydro-acetonic extract, both extracts superior to BHT (0.122 ± 0.021 g/mL). While the FRAP assay showed a low iron-reducing power values for both extracts compared to BHT), the overall antioxidant activity of the two extracts was found to be considerable. The overall antioxidant activity of the hydro-ethanolic extract was 8.95 ± 0.42 mg EAA/g, whereas the total antioxidant activity of the hydro-acetonic extract was 6.44 ± 0.61 mg EAA/g. In comparison with the antibiotic Erythromycin, HAE and HEE from S. elaeagnifolium leaves demonstrated significant antibacterial action. HAE had the best inhibitory efficacy against Bacillus subtilis DSM 6333, with an inhibition diameter of 10.5 ± 0.50 mm and a MIC of 7.5 ± 0.00 mg/mL, as well as against Proteus mirabilis ATCC 29906, with an inhibitory diameter of 8.25 ± 0.75 mm and a MIC of 15 ± 0.00 mg/mL.
The biological activities of propolis samples are the result of many bioactive compounds present in the propolis. The aim of the present study was to determine the various chemical compounds of some ...selected propolis samples collected from Palestine and Morocco by the High-Performance Liquid Chromatography–Photodiode Array Detection (HPLC-PDA) method, as well as the antioxidant and antibacterial activities of this bee product. The chemical analysis of propolis samples by HPLC-PDA shows the cinnamic acid content in the Palestinian sample is higher compared to that in Moroccan propolis. The results of antioxidant activity demonstrated an important free radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH); 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and reducing power assays) with EC50 values ranging between 0.02 ± 0.001 and 0.14 ± 0.01 mg/mL. Additionally, all tested propolis samples possessed a moderate antibacterial activity against bacterial strains. Notably, Minimum Inhibitory Concentrations (MICs) values ranged from 0.31 to 2.50 mg/mL for Gram-negative bacterial strains and from 0.09 to 0.125 mg/mL for Gram-positive bacterial strains. The S2 sample from Morocco and the S4 sample from Palestine had the highest content of polyphenol level. Thus, the strong antioxidant and antibacterial properties were apparently due to the high total phenolic and flavone/flavonol contents in the samples. As a conclusion, the activities of propolis samples collected from both countries are similar, while the cinnamic acid in the Palestinian samples was more than that of the Moroccan samples.
The world is currently grappling with the coronavirus disease (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The infection can cause fever, a dry cough, ...fatigue, severe pneumonia, respiratory distress syndrome, and in some cases death. There is currently no effective antiviral SARS-CoV-2 drug. To reduce the number of infections and deaths, it is critical to focus on strengthening immunity. This review aims to conduct a comprehensive search on the previous studies using Google Scholar, ScienceDirect, Medline, PubMed, and Scopus for the collection of research papers based on the role of zinc in the immune system, the antiviral activity of zinc, the effect of zinc supplementation in respiratory infections, the therapeutic approaches against viral infections based on medicinal plants, and the role of plants’ bioactive molecules in fighting viral infections. In conclusion, we highlighted the pivotal role of zinc in antiviral immunity and we suggested the bioactive molecules derived from medicinal plants as a search matrix for the development of anti-SARS-CoV-2 drugs.
Bee products are known for their beneficial properties widely used in complementary medicine. This study aims to unveil the physicochemical, nutritional value, and phenolic profile of bee pollen and ...honey collected from Boulemane–Morocco, and to evaluate their antioxidant and antihyperglycemic activity. The results indicate that Citrus aurantium pollen grains were the majority pollen in both samples. Bee pollen was richer in proteins than honey while the inverse was observed for carbohydrate content. Potassium and calcium were the predominant minerals in the studied samples. Seven similar phenolic compounds were found in honey and bee pollen. Three phenolic compounds were identified only in honey (catechin, caffeic acid, vanillic acid) and six phenolic compounds were identified only in bee pollen (hesperidin, cinnamic acid, apigenin, rutin, chlorogenic acid, kaempferol). Naringin is the predominant phenolic in honey while hesperidin is predominant in bee pollen. The results of bioactivities revealed that bee pollen exhibited stronger antioxidant activity and effective α-amylase and α-glycosidase inhibitory action. These bee products show interesting nutritional and bioactive capabilities due to their chemical constituents. These features may allow these bee products to be used in food formulation, as functional and bioactive ingredients, as well as the potential for the nutraceutical sector.
Background: In the realms of herbal pharmacology, the Apiaceae family has long held the torch, with parsley, coriander, and celery at the forefront. They have been extensively utilized in traditional ...therapies, including their use as aphrodisiacs. The primary objective of this study was to evaluate the aphrodisiac potential of three Apiaceae plants: Petroselinum crispum, Coriandrum sativum, and Apium graveolens, both individually and in combination, to substantiate their traditional medicinal usage. Methods: The administration of extracts orally (at a dose of 250 mg/kg of body weight) was carried out daily for 15 days in various groups of male rats. To induce receptivity in female rats used for mating, hormonal treatment was employed. The study encompassed the assessment of general mating behavior, which was compared to that induced by the standard reference drug, sildenafil citrate. Additionally, hematological and biochemical assays were conducted. Results: The results of this study demonstrated that oral administration of the extracts at a dose of 250 mg/kg significantly heightened sexual activity in male rats. This was characterized by a substantial increase in parameters such as mounting frequency, erection frequency, ejaculation frequency, and copulatory rate, coupled with a noteworthy reduction in mounting latency, intromission latency, and latency time. However, when compared to the positive control group (sildenafil), the polyherbal formulation did not induce significant alterations in hematological parameters such as red blood cell count, white blood cell count, blood platelet count, and hemoglobin levels. Furthermore, the hepatic and renal markers in treated rats displayed no significant disturbances when administered with the individual extracts of the three Apiaceae plants or in combination, in comparison to the control group. The results also unveiled a substantial augmentation effect in blood testosterone levels following the individual administration of Apiaceae plant extracts when compared to the positive control group. The combined administration of these extracts at a dose of 250 mg/kg exhibited an even more pronounced effect in enhancing testosterone levels than administering the individual extracts. Conclusions: In conclusion, the polyherbal formulation comprising these Apiaceae plants demonstrated a substantial and sustained enhancement in the sexual activity of healthy male rats, without any discernible adverse effects. This suggests that the formulation maintains aphrodisiac properties, possibly attributed to its nerve-stimulating effects, thereby enhancing libido and potency. These findings not only support the traditional use of these herbs for their aphrodisiac properties but also propose potential applications for human use as a natural alternative for addressing male sexual disorders and as aphrodisiac agents.
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Acorn oil has been receiving increasing attention due to its nutritional potentials. However, its application as a novel food ingredient has not yet been fully explored. This paper summarizes ...chemical composition, extraction methods, potential health benefits, and current applications of acorn oil, with the aim of providing suggestions for its exploitation. Acorn oil is an excellent source of essential fatty acids (oleic, linoleic, α-linolenic, and palmitic acids). Acorns are a rich source of tocopherols, with γ-tocopherol being the most abundant. It also contains various bioactive compounds such as polyphenols and sterols (mainly β-sitosterol). Diets enriched with acorn oil can be beneficial in preventing cardiovascular disease (CVD), cancer, and type 2 diabetes as well as offer antioxidant activity. Further studies should focus on producing better quality acorn oil such as the application of more innovative and optimized techniques that can increase its health benefits and hence utilization.
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