Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the predominant protein in photosynthesizing plant parts and the most abundant protein on earth. Amino acids deriving from its net ...degradation during senescence are transported to sinks (e.g. developing leaves, fruits). Rubisco catabolism is not controlled only by the overall sink demand. An accumulation of carbohydrates may also accelerate senescence and Rubisco degradation under certain conditions. Amino acids produced by proteolysis are rapidly redistributed in plants with proper source-sink relationships. In leaves of wheat plants with reduced sink capacity (e.g. sink removal, phloem interruption by steam girdling at the leaf base), Rubisco is degraded and free amino acids accumulate. They may be washed out in the rain during late senescence. In leaves of depodded soybeans, Rubisco is degraded and amino acids can be reutilized in these leaves for the synthesis of special vacuolar proteins in the paraveinal mesophyll (vegetative storage proteins). Nitrogen deriving from Rubisco degradation in older (senescing) leaves of annual crops is integrated to some extent again in newly synthesized Rubisco in younger leaves or photosynthesizing tissues of fruits. Finally, a high percentage of this nitrogen is accumulated in protein bodies (storage proteins). At the subcellular level, Rubisco can be degraded in intact chloroplasts. Reactive oxygen species may directly cleave the large subunit or modify it to become more susceptible to proteolysis. A metalloendopeptidase may play an important role in Rubisco degradation within intact chloroplasts. Additionally, the involvement of vacuolar endopeptidase(s) in Rubisco catabolism (at least under certain conditions) was postulated by various laboratories.
Microplastics are a global concern, and yet, Philippine studies about it are still wanting. This study investigated microplastic ingestion among detritus-feeding mullet versus the herbivorous ...rabbitfish, and between freshwater and marine fishes. The first part aimed to compare microplastics in fishes’ guts differing in feeding guilds, individual sizes, and body weights. The second part compared MPs in fishes’ guts and their surrounding waters. Part one revealed that herbivores (58.57%) ingested more MPs than their detritivore-counterparts (30.0%). Pearson correlation, averaging 0.06, suggests a weak correlation between fish weight and MPs amount for both species. Part two showed marine fishes (66.0%) ingested more MPs than its freshwater counterparts (45.0%). A very weak correlation was observed between fish weight and amount of MPs ingested. Fish along the estuary ingested more MPs than those in other stations. No significant differences (p = 0.23) between the MPs in the water samples from each sampling stations was found.
The interaction of tetramethylammonium acetate with (MeCN)2Ni(CF3)2, (MeCN)2Ni(C2F5)2, and NMe4(MeCN)Ni(CF3)3 was explored by 19F NMR spectroscopy. We show that depending on the nature of the ...nickel complex, one or two acetate ligands can add to the metal center and replace the nickel-bound MeCN ligands, depending on the acetate concentration. The number of acetates that could bind to nickel, and whether the resulting complex exists as a monomer or dimer, was determined to be dependent on the nature of the fluoroalkyl ligand. Moreover, we observe that oxidation of the nickel center of (MeCN)2Ni(CF3)2 in the presence of two equivalents of acetate leads cleanly to the octahedral, paramagnetic (EPR spectroscopy), and anionic Ni(III) complex NMe4Ni(CF3)2(OAc)2.
To help address the need for preventive measures against dengue fever, a leading cause of child mortality in the Philippines, vaccine trials are ongoing and a tetravalent vaccine (Dengvaxia™, Sanofi ...Pasteur) has been developed. It is hypothesized that while acceptability would be high among primary caregivers (i.e., parents/guardians), the willingness to have one's child immunized against dengue would be associated with socio-demographic variables, attitudes and knowledge regarding dengue and vaccination, and past experience with dengue. This study aimed to assess the aforementioned factors’ association with primary caregivers’ willingness to avail of a dengue vaccine for their 9 to 14-year-old children in an urban community in the Philippines.
A cross-sectional study utilizing interviews was conducted to determine which factors were associated with willingness-to-avail assuming a free vaccine, and a case study utilizing a focus group discussion was employed to capture some underlying reasons for their willingness. Data were analyzed using multiple logistic regression and thematic analysis.
Among the 202 study participants, 193 (95.54%) were willing to avail of the vaccine. There was a high probability of vaccine acceptance by primary caregivers (95.54%), with good attitude towards vaccination (≥12/15 points) aOR 10.62, 90% CI (1.73–26.28) and large household size (>5) aOR 9.63, 90% CI (2.04–45.58) being positively associated with willingness-to-avail, and good knowledge regarding dengue fever aOR 0.10, 90% CI (0.03–0.74) and older age (>44 years) aOR 0.14, 90% CI (0.03–0.61) being negatively associated.
Crude analysis showed that household size, knowledge regarding dengue, and attitude towards vaccination were significantly associated with willingness. Multivariate analysis revealed that these factors and the primary caregiver’s age were associated with willingness. Thematic analysis showed various perceptions regarding dengue and vaccination. Knowing these factors are associated with willingness-to-avail of the vaccine may help in understanding the audience of health promotion projects aimed at increasing immunization coverage.
Nowadays, retailers are embracing the Internet of Things as the latest technology to drive superior customer experience. Leverage data sources from sensors, beacons and mobile devices to identify and ...analyze in-store customer shopping behavior. With this motivation, this study implemented an in-store customer traffic and path monitoring system for supermarket using image processing and object detection. the system utilized the ultra-wideband indoor positioning technique to monitor the customer shopping path and the single shot multibox detection technique to monitor the real-time customer traffic. The customer monitoring system was implemented and evaluated in an actual small-scale supermarket. Results showed that the detection model prediction score and the traffic counting both obtained an accuracy score of 99%. In addition, the localization system achieved the minimum error difference of 9.73% for x coordinate and 3.86% for y coordinate between pre-determined positions and the actual anchor position readings. Furthermore, the system successfully generated the most frequent path and the total customer traffic of the day. In the future, this work can aid retail owners make better choices, run businesses more efficiently, and deliver improved customer service.
Despite the effort to eradicate rabies in the Philippines, human rabies cases have not decreased in the past decade. Rabid dogs pose the most significant hazard in the countries with the highest ...burden of rabies, and 70% rabies vaccine coverage is recommended for dogs in high-risk areas. Ascertaining the owned dog population and community knowledge on rabies can help improve vaccine coverage and information campaigns.
We conducted a cross-sectional survey in six randomly selected communities (five urban, one rural) in Central Luzon, Philippines. We first conducted the complete mapping of 9,173 households and then randomly selected 727 households. More than half (54.1%) of the households owned dogs (1.21 dogs/household). In the 727 households, we identified 878 owned dogs and 3256 humans. According to these results, the dog-to-human ratio was approximately 1:3.7. Only 8.8% of households reported a history of dog bite in 2019. Among dog-owning households, 31% reported that they allow their dogs to roam freely. Of the recorded dogs, 35.9% have never been vaccinated, and only 3.5% were spayed or castrated. Factors associated with lower rabies knowledge include (1) no education aOR: 0.30 (0.16-0.59), and (2) only primary school education aOR: 0.33 (0.22-0.49). In contrast, factors associated with higher knowledge include (1) owning a dog and not allowing them to roam freely aOR: 2.01 (1.41-2.87) and (2) owning a dog and allowing them to roam freely aOR: 1.84 (1.17-2.92), when compared to those with no dogs.
We identified a larger dog population in the community than the usual estimates (1:10), suggesting that annual vaccine needs in the Philippines must be reassessed. Our survey shows a relatively good understanding of rabies; however, awareness of the concept of rabies as a disease, and how animals and humans can acquire it, is lacking.
Abstract The idea that the environmental toxin β- N -methylamino- l -alanine (BMAA) is involved in neurodegenerative diseases on Guam has risen and fallen over the years. The theory has gained ...greater interest with recent reports that BMAA is biomagnified, is widely distributed around the planet, and is present in the brains of Alzheimer's patients in Canada. We provide two important new findings. First, we show that BMAA at concentrations as low as 10 μM can potentiate neuronal injury induced by other insults. This is the first evidence that BMAA at concentrations below the mM range can enhance death of cortical neurons and illustrates potential synergistic effects of environmental toxins with underlying neurological conditions. Second, we show that the mechanism of BMAA toxicity is threefold: it is an agonist for NMDA and mGluR5 receptors, and induces oxidative stress. The results provide further support for the hypothesis that BMAA plays a role in neurodegenerative diseases.
Modern jeepneys represent a notable step forward in the Philippines’ modernization efforts. However, there is a need for further study since this has caused a lot of criticism in the country, and no ...studies have established its service quality and the behavioral intentions among passengers. This study assessed the factors influencing passengers’ intention to use modern jeepneys, employing a higher-order reflective construct using partial least square structural equation modeling. An online questionnaire gathered data from 502 commuters using modern jeepneys, employing convenience sampling for a diverse sample. Significant relationships (p-value < 0.05) were found between service quality (β: 0.559), attitude (β: 0.169), perceived behavioral control (β: 0.183), and behavioral intentions (β: 0.160), with assurance emerging as the most influential factor for higher-ordered constructs (β: 0.869). Meanwhile, satisfaction’s significant influence on behavioral intention was highest for lower-ordered constructs (β: 0.673). The study underscored the importance of building trust with passengers, prioritizing safety and security, and integrating modern jeepneys with traditional designs to enhance commuter experiences and promote modernized and sustainable transportation alternatives. It highlights the critical role of providing high-quality service in fostering positive intentions toward using modern jeepneys and its utilization. Despite the backlash of the majority on the redevelopment of traditional jeepneys, results presented that passengers are more inclined to use public transportation based on travel experiences rather than societal approval. The study offers theoretical and practical implications for future works and government organizations, transportation management, and modern jeepney manufacturers, emphasizing the importance of improving service quality.
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