Objectives. To evaluate the inflammatory status and the cartilage regenerative potential of pathological synovial fibroblasts from patients with osteoarthritis (OA) compared with non-inflamed ...synovium (NS)-derived cells from patients with chondropathy. Methods. The inflammatory cell phenotype was investigated based on the constitutive and inducible surface expression and secretion of various effector molecules using flow cytometry or ELISA assays. The capacity of cells to produce cartilage-like extracellular matrix was assessed using acid Alcian blue staining and type II collagen immunostaining after treatment with transforming growth factor β1 (TGF-β1). Results. OA and NS fibroblasts consistently expressed CD29, CD44, CD49e, CD54, CD90 and CD106. Expression of high-affinity receptors for IL-4, IL-15, CXCL8 and CXCL12 was also detected but only intracellularly. All types of fibroblasts spontaneously released abundant amounts of CXCL12, CCL2, IL-6 and tissue inhibitor of metalloproteinase 1, while the production of IL-11, TGF-β1, matrix metalloproteinase 1 (MMP-1) and MMP-9 was detected at moderate levels. Several other secreted factors remained undetectable. No statistically significant differences were noted between the two groups of fibroblasts. Treatment with the proinflammatory cytokine tumour necrosis factor α (TNF-α) up-regulated the same set of surface and secreted molecules, including CD54, CD106, membrane IL-15, CCL2 and CCL5. Under TGF-β1 treatment and adipogenic culture conditions, both OA and NS fibroblasts displayed chondrogenic and adipocytic activities that were reduced in OA compared with NS cells. Conclusions. OA synovial fibroblasts did not display a distinct activated inflammatory phenotype compared with NS cells. However, they did differ in their reduced ability to produce cartilage-like matrix. This difference may be an additional important factor contributing to OA pathogenesis.
The purpose of this study was to determine whether imbalance of the internal and external rotator musculature of the shoulder were etiological factors implicated in impingement syndrome. Shoulder ...torque measurements were obtained from 15 asymptomatic volunteers and 30 patients with chronic impingement syndrome, 15 of whom were evaluated after arthroscopic anterior acromioplasty. Isokinetic strength was assessed using the Biodex system in a modified position (in the plane of the scapula and in 45 degrees abduction) with test speeds of 60 degrees and 180 degrees per second. Internal and external rotator strength values and ratios were calculated for both peak torque and average power. Shoulder rotational strength values and the internal rotator/external rotator ratio were significantly higher in the dominant and nondominant control group shoulders than in the involved and uninvolved impingement shoulders for operated on and nonoperated on patients. These data demonstrate that primary change in the normal internal rotator/external rotator ratio of the shoulder is an etiological factor implicated in impingement syndrome not modified by anterior acromioplasty.
Abstract The exploration of the human microbiome was recently revolutionized by microbial culturomics and taxonogenomics. Thanks to this approach, we report here the main characteristics of ...Romboutsia timonensis strain Marseille-P326, a new bacterium isolated from the right human colon by colonoscopy in a 63-year-old French man with severe anaemia with melaena.
SARS‐CoV‐2 infection presenting as a febrile rash Amatore, F.; Macagno, N.; Mailhe, M. ...
Journal of the European Academy of Dermatology and Venereology,
July 2020, Letnik:
34, Številka:
7
Journal Article
The authors stress the prevalence and severity of wrist involvement in rheumatoid disease. Improved knowledge of aetiopathogenesis and deformities had led to identification of the importance of the ...distal consequences on the digital joints of decentralization of the wrist. Synovectomy retains an important place at the onset of the disease. This may be completed by stabilization procedures (tendon transfer and partial arthrodesis). At the advanced stage of the disease, arthroplasty is currently overtaking arthrodesis. Interposition arthroplasty, with its rare indications, is now well developed. Although the solution of the future, arthroplasties lack sufficient follow up in terms of their results. The Swanson implant offers limited mobility but ensures freedom from pain and a good wrist axis. The ideal treatment of the rheumatoid wrist is oriented towards early conservative surgery and later, at an advanced stage, towards arthroplasty, the ideal technique of which remains to be discovered.
Thirty-one carpal tunnel syndromes were seen in 20 patients treated by haemodialysis for renal failure. At the time of operation, the duration of the haemodialysis was an average of 9 years. On 24 ...occasions, the syndrome existed in the limb of the arterio venous fistula but on 7 occasions it only appeared in the opposite limb. The syndrome is characterised by the development of, or the worsening of paraesthesiae during haemodialysis sessions. The degree and duration of the numbness in the fingers in the morning was particularly striking. At surgery, the nerve was always obviously narrowed. It was noted in 21 cases that there were greyish granulation in the synovium and sometimes intra tendinous nodules. All the operated cases improved. The pathogenesis is discussed in relation to amyloid deposits. The haemodynamic role of the fistula seems to be to make the development of the lesion more likely. Microscopic examination and biochemical studies give rise to the conclusion that this is an AL type of amyloidosis.
Active patient participation in the operative and postoperative programme is very important for tenolysis, tendon grafts and joint releases. Different anesthesic means can be used to perform ...selective sensitive analgesia. Cervical epidural anesthesia was used for fifteen cases of hand surgery. The technique is described, the results are analyzed. Indications and use of cervical epidural anesthesia for hand surgery are discussed.
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