Background: Since cyclosporin A (CsA)‐induced overgrowth seldom occurs at sites distant from teeth, the periodontal ligament has been considered significant. The aim of this study was to examine ...overgrowth occurrence at the edentulous ridge—the sites without the ligament—after CsA therapy in rats.
Methods: After extracting all right maxillary molars, 16 Sprague‐Dawley rats underwent a 2‐week healing period. The animals were separated into CsA and control groups. CsA rats received 15 mg/kg of CsA by gastric feeding for 4 weeks, while the control group received only mineral oil. At the end of study, all animals were sacrificed and stone models were immediately obtained by rubber‐based impressions. The edentulous ridge morphology, including the bucco‐lingual width and the vertical height, was measured on the models. For histometry, 10 sections were selected from the edentulous ridge of each animal after undecalcified tissue preparation. The soft tissue areas of the edentulous ridge and the trabecular bone morphology of the dental alveolus were measured.
Results: CsA therapy produced a significant increase of the ridge width and height, measured from the stone models, when compared to the control group. Under histometry, CsA resulted in a significant increase of the epithelium, connective tissue, and total soft tissue areas. The measured trabecular bone volume was affected by both examining factors: the drug therapy and the location of the dental alveolus. CsA therapy produced a significant loss of bone volume but a significant increase of the bone‐specific surface area. Although the mean osteoid volume was similar between CsA and control groups, a significant decrease of the fractional formation surface in the CsA group was revealed.
Conclusions: An enlarged edentulous ridge and an altered dental alveolar bone morphology were observed in CsA‐treated animals at the end of the study; therefore, we suggest that CsA may induce not only a soft tissue overgrowth but also an alveolar bone alteration at the edentulous ridge. The hypothesis that tooth or periodontal ligament is an essential component for the overgrowth development is questioned. J Periodontol 2001;72:889‐894.
Diamond-like carbon (DLC) films were prepared on magnetic disk surfaces using an electron cyclotron resonance assisted microwave plasma chemical vapor deposition (ECR-MPCVD) system with variable ...radio-frequency (r.f.) substrate bias. Surface roughness of DLC deposited on hard disks was investigated by atomic force microscopy (AFM), which revealed that the DLC coated surfaces are smoother than those of the uncoated disks. Nitrogen incorporation into the films (a-C:N) further reduced the root-mean-square (RMS) roughness to 2.5 angstrom for films prepared at a substrate bias of -120 V. Scratch resistance was improved when the DLC coatings were deposited at bias voltages greater than -90 V. However, the nitrogen introduction into the films deteriorated their scratch resistance. When the DLC films were subjected to an accelerated corrosive environment, pinhole density remarkably varied with the deposition conditions. The DLC films deposited with lower substrate biases and nitrogen incorporation resulted in poorer corrosion resistance. Internal stress, hydrogen content and graphitic cluster size of these films were correlated with data acquired by Raman analysis.
Previously, 4 cases of myelodysplastic syndrome were reported that had unusual, distinct monocytic nodules in bone marrow. The monocytic nodules, predominantly composed of monocytes with CD68+ ...immunostaining, had no or low expression of Ki-67 and topoisomerase II alpha. The purpose of the present study was to further define the associated clinical diseases, histologic features, and immunohistochemical characteristics of 21 such cases. Relevant hematopathologic slides of all cases were reviewed, and extensive immunohistochemical staining was performed. Most patients (15/21 71%) had monocytosis in the bone marrow, and chronic myelomonocytic leukemia was the most commonly associated clinical disease. In 4 patients, the monocytic nodules also were present in lymph node, spleen, or skin. Immunohistochemical staining results for the monocytes in the nodules were similar to those for plasmacytoid monocytes. Our study established that monocytic nodules can be present in myelodysplastic syndromes, myeloproliferative diseases, and acute myeloid leukemia; verified the monocytic lineage; and revealed the low proliferative state of these cells.
The China Dark Matter Experiment Collaboration reports the first experimental limit on weakly interacting massive particles (WIMPs) dark matter from 14.6 kg-days of data taken with a 994 g p-type ...point-contact germanium detector at the China Jinping underground laboratory where the rock overburden is more than 2400 m. The energy threshold achieved was 400 eVee. According to the 14.6 kg-day live data, we placed the limit of sigma sub( chi )N = 1.75 x 10 super(-40) cm super(2) at a 90% confidence level on the spin-independent cross section at a WIMP mass of 7 GeV before differentiating bulk signals from the surface backgrounds.
In-situ morphological evolution of displacement in pouch-type commercial lithium-ion batteries during multiple fifty-five electrochemical charging-discharging cycles was measured via digital image ...correlation technique. The maximum principal strain on the battery surface reached 0.35% during 55 cycles. The whole volume change analysis of LIBs shows that the maximum volume change rate arrives at 4.27% at the fully 52nd charging end, and the maximum residual volume change rate is about 2.89% at the 54th discharging end. The surface morphologies of cathodes and anodes before and after electrochemical cycling were observed by scanning electron microscopy. The elastic modulus of the copper foil in LIBs decrease from as-received 16.7 GPa to 10.6 GPa after 55 cycles by using tensile tests.
On the potential energy surface, 12 isomers of H2CO3 are located. Their molecular properties such as geometries, vibrational frequencies, rotational constants, dipole moments, gas-phase acidities, ...and relative energies are calculated. Various reaction pathways and decomposition products have also been discussed. Among these products, CO2 and H2O are definitely the most favorable products with predominant abundance. Large energy barriers are predicted for other dissociation channels leading to the formation of oxygen, formaldehyde, and so on. These high energy channels are not important thermodynamically and kinetically, but they might occur in the presence of cosmic rays in astronomic environments. From the present work we suggest that chemical reactions between CO2 and H2O at the polar ice caps could be a potential source of H2CO and O2, in addition to the previously proposed mechanisms, i.e., the oxidation of methane and cosmic-ray-mediated production through the intermediate H2CO3. The results of the present work may provide useful data to improve our understanding of icy chemistry at the polar caps on Mars.
Objective
Our aim is to evaluate the expression of
SATB
1 in human oral squamous cell carcinomas (
OSCC
) and its role in the invasiveness and metastasis of
OSCC
.
Subjects and methods
A human
OSCC
...tissue microarray was used to evaluate the expression pattern of
SATB
1.
SATB
1
mRNA
knockdown was performed in human
OSCC
cell lines
SCC
25 and Cal27 to assess the function of
SATB
1 in the invasiveness and metastasis of
OSCC
.
Results
SATB
1 is highly expressed in human
OSCC
determined by immunohistochemistry, and its nuclear/cytoplasmic ratio of histoscore is significantly correlated with patients’ prognosis. Reduced cell motility, invasiveness, expression of epithelial to mesenchymal transition (
EMT
) markers (N‐cadherin and β‐catenin), and elevated expression of epithelial markers were observed in
SATB
1‐knockdown cells in
in vitro
studies. Depletion of
SATB
1 also restored a cobblestone‐like morphology in
TGF
‐β1‐treated cells.
Conclusions
These findings suggest
SATB
1 may play an important role in
OSCC
invasiveness and metastasis.
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