The sterile insect technique (SIT) is an environmentally safe and proven technology to suppress wild populations. To further advance its utility, a novel CRISPR-based technology termed precision ...guided SIT (pgSIT) is described. PgSIT mechanistically relies on a dominant genetic technology that enables simultaneous sexing and sterilization, facilitating the release of eggs into the environment ensuring only sterile adult males emerge. Importantly, for field applications, the release of eggs will eliminate burdens of manually sexing and sterilizing males, thereby reducing overall effort and increasing scalability. Here, to demonstrate efficacy, we systematically engineer multiple pgSIT systems in Drosophila which consistently give rise to 100% sterile males. Importantly, we demonstrate that pgSIT-generated sterile males are fit and competitive. Using mathematical models, we predict pgSIT will induce substantially greater population suppression than can be achieved by currently-available self-limiting suppression technologies. Taken together, pgSIT offers to potentially transform our ability to control insect agricultural pests and disease vectors.
The use of a site-specific homing-based gene drive for insect pest control has long been discussed, but the easy design of such systems has become possible only with the recent establishment of ...CRISPR/Cas9 technology. In this respect, novel targets for insect pest management are provided by new discoveries regarding sex determination. Here, we present a model for a suppression gene drive designed to cause an all-male population collapse in an agricultural pest insect. To evaluate the molecular details of such a sex conversion-based suppression gene drive experimentally, we implemented this strategy in Drosophila melanogaster to serve as a safe model organism. We generated a Cas9-based homing gene-drive element targeting the transformer gene and showed its high efficiency for sex conversion from females to males. However, nonhomologous end joining increased the rate of mutagenesis at the target site,which resulted in the emergence of drive-resistant alleles and therefore curbed the gene drive. This confirms previous studies that simple homing CRISPR/Cas9 gene-drive designs will be ineffective. Nevertheless, by performing population dynamics simulations using the parameters we obtained in D. melanogaster and by adjusting the model for the agricultural pest Ceratitis capitata, we were able to identify adequate modifications that could be successfully applied for the management of wild Mediterranean fruit fly populations using our proposed sex conversion-based suppression gene-drive strategy.
We perform a search for stellar streams around the Milky Way using the first 3 yr of multiband optical imaging data from the Dark Energy Survey (DES). We use DES data covering ∼5000 deg2 to a depth ...of g > 23.5 with a relative photometric calibration uncertainty of <1%. This data set yields unprecedented sensitivity to the stellar density field in the southern celestial hemisphere, enabling the detection of faint stellar streams to a heliocentric distance of ∼50 kpc. We search for stellar streams using a matched filter in color-magnitude space derived from a synthetic isochrone of an old, metal-poor stellar population. Our detection technique recovers four previously known thin stellar streams: Phoenix, ATLAS, Tucana III, and a possible extension of Molonglo. In addition, we report the discovery of 11 new stellar streams. In general, the new streams detected by DES are fainter, more distant, and lower surface brightness than streams detected by similar techniques in previous photometric surveys. As a by-product of our stellar stream search, we find evidence for extratidal stellar structure associated with four globular clusters: NGC 288, NGC 1261, NGC 1851, and NGC 1904. The ever-growing sample of stellar streams will provide insight into the formation of the Galactic stellar halo, the Milky Way gravitational potential, and the large- and small-scale distribution of dark matter around the Milky Way.
Shear peak statistics has gained a lot of attention recently as a practical alternative to the two-point statistics for constraining cosmological parameters. We perform a shear peak statistics ...analysis of the Dark Energy Survey (DES) Science Verification (SV) data, using weak gravitational lensing measurements from a 139 deg super( 2) field. We measure the abundance of peaks identified in aperture mass maps, as a function of their signal-to-noise ratio, in the signal-to-noise range ... To predict the peak counts as a function of cosmological parameters, we use a suite of N-body simulations spanning 158 models with varying ... and ..., fixing ..., to which we have applied the DES SV mask and redshift distribution. In our fiducial analysis we measure ..., after marginalizing over the shear multiplicative bias and the error on the mean redshift of the galaxy sample. We introduce models of intrinsic alignments, blending and source contamination by cluster members. These models indicate that peaks with ... would require significant corrections, which is why we do not include them in our analysis. We compare our results to the cosmological constraints from the two-point analysis on the SV field and find them to be in good agreement in both the central value and its uncertainty. We discuss prospects for future peak statistics analysis with upcoming DES data. (ProQuest: ... denotes formulae/symbols omitted.)
We report the results from a search for z > 6.5 quasars using the Dark Energy Survey (DES) Year 3 data set combined with the VISTA Hemisphere Survey (VHS) and WISE All-Sky Survey. Our photometric ...selection method is shown to be highly efficient in identifying clean samples of high-redshift quasars, leading to spectroscopic confirmation of three new quasars – VDES J0244-5008 (z = 6.724), VDES J0020-3653 (z = 6.834), and VDES J0246-5219 (z = 6.90) – which were selected as the highest priority candidates in the survey data without any need for additional follow-up observations. Here, we have obtained spectroscopic observations in the near-infrared for VDES J0244-5008 and VDES J0020-3653 as well as our previously identified quasar, VDES J0224-4711 at z = 6.50 from Reed et al. We use the near-infrared spectra to derive virial black hole masses from the full width at half-maximum of the Mg ii line. These black hole masses are ≃1–2 × 109 M⊙. Combined with the bolometric luminosities of these quasars of Lbol ≃ 1–3 × 1047, these imply that the Eddington ratios are high, ≃0.6–1.1. We consider the C iv emission line properties of the sample and demonstrate that our high-redshift quasars do not have unusual C iv line properties when compared to carefully matched low-redshift samples. Our new DES + VHS z > 6.5 quasars now add to the growing census of luminous, rapidly accreting supermassive black holes seen well into the epoch of reionization.
is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control ...methods, the development of innovative control measures has become of paramount importance. The use of gene drives has sparked significant enthusiasm for genetic control of mosquitoes; however, no such system has been developed in
. To fill this void, here we develop several CRISPR-based split gene drives for use in this vector. With cleavage rates up to 100% and transmission rates as high as 94%, mathematical models predict that these systems could spread anti-pathogen effector genes into wild populations in a safe, confinable and reversible manner appropriate for field trials and effective for controlling disease. These findings could expedite the development of effector-linked gene drives that could safely control wild populations of
to combat local pathogen transmission.
Large-scale integrated quantum photonic technologies will require on-chip integration of identical photon sources with reconfigurable waveguide circuits. Relatively complex quantum circuits have been ...demonstrated already, but few studies acknowledge the pressing need to integrate photon sources and waveguide circuits together on-chip. A key step towards such large-scale quantum technologies is the integration of just two individual photon sources within a waveguide circuit, and the demonstration of high-visibility quantum interference between them. Here, we report a silicon-on-insulator device that combines two four-wave mixing sources in an interferometer with a reconfigurable phase shifter. We configured the device to create and manipulate two-colour (non-degenerate) or same-colour (degenerate) path-entangled or path-unentangled photon pairs. We observed up to 100.0 ± 0.4% visibility quantum interference on-chip, and up to 95 ± 4% off-chip. Our device removes the need for external photon sources, provides a path to increasing the complexity of quantum photonic circuits and is a first step towards fully integrated quantum technologies.
The Twentieth Century Reanalysis Project Compo, G. P.; Whitaker, J. S.; Sardeshmukh, P. D. ...
Quarterly journal of the Royal Meteorological Society,
January 2011 Part A, 2011, 2011-01-00, 20110101, 2011-01-01, Letnik:
137, Številka:
654
Journal Article
Abstract
The mosquito
Aedes aegypti
is the principal vector for arboviruses including dengue/yellow fever, chikungunya, and Zika virus, infecting hundreds of millions of people annually. ...Unfortunately, traditional control methodologies are insufficient, so innovative control methods are needed. To complement existing measures, here we develop a molecular genetic control system termed precision-guided sterile insect technique (pgSIT) in
Aedes aegypti
. PgSIT uses a simple CRISPR-based approach to generate flightless females and sterile males that are deployable at any life stage. Supported by mathematical models, we empirically demonstrate that released pgSIT males can compete, suppress, and even eliminate mosquito populations. This platform technology could be used in the field, and adapted to many vectors, for controlling wild populations to curtail disease in a safe, confinable, and reversible manner.
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