We report on a new analysis of neutrino oscillations in MINOS using the complete set of accelerator and atmospheric data. The analysis combines the \(\nu_{\mu}\) disappearance and \(\nu_{e}\) ...appearance data using the three-flavor formalism. We measure \(|\Delta m^{2}_{32}|=2.28-2.46\times10^{-3}\mbox{\,eV}^{2}\) (68% C.L.) and \(\sin^{2}\theta_{23}=0.35-0.65\) (90% C.L.) in the normal hierarchy, and \(|\Delta m^{2}_{32}|=2.32-2.53\times10^{-3}\mbox{\,eV}^{2}\) (68% C.L.) and \(\sin^{2}\theta_{23}=0.34-0.67\) (90% C.L.) in the inverted hierarchy. The data also constrain \(\delta_{CP}\), the \(\theta_{23}\) octant degeneracy and the mass hierarchy; we disfavor 36% (11%) of this three-parameter space at 68% (90%) C.L.
We present a direct measurement of trilinear gauge boson couplings at gamma WW and ZWW vertices inWW and WZ events produced in p (p) over bar collisions at root s = 1:96 TeV. We consider events with ...one electron or muon, missing transverse energy, and at least two jets. The data were collected using the D0 detector and correspond to 1:1 fb(-1) of integrated luminosity. Considering two different relations between the couplings at the gamma WW and ZWW vertices, we measure these couplings at 68% C.L. to be kappa(gamma) = 1.07(-0.29)(+0.26) lambda = 0.00(-0.06)(+0.00), and g(1)(Z) = 1.04(-0.09)(+0.09) in a scenario respecting SU(2)(L) circle times U(1)(Y) gauge symmetry and kappa = 1.04(-0.11)(+0.11) and lambda = 0.00(-0.06)(+0.06) in an "equal couplings'' scenario.
In this report, the regulatable expression by tetracycline of the rat recombinant P2X(3) receptor in stably transfected Chinese hamster ovary (CHO-K1) expressing the tetracycline-controlled ...transactivator (tTA) is described. cDNA encoding the rat P2X(3)-receptor was subcloned into pTRE (a tetracycline-repressible expression vector) which was used to transfect stably CHO-K1 tTA cells. Using whole cell patch clamp techniques, 100 microM ATP evoked inward currents of 2.9+/-1.6 nA in transfected cells grown in the absence of tetracycline (tet-). The P2X(3) receptor protein was detectable by immunoblot as early as 24 h and protein expression levels continued to increase as much as 192 h following activation of tTA by the removal of the antibiotic. Saturation binding isotherms using 35SATP gamma S yielded a pK(d) of 8.2+/-0.1 and a B(max) of 31.9+/-3.5 pmol/mg protein in tet- cell membranes and a pK(d) of 8.1+/-0.1 and a B(max) of 5.8+/-0.8 pmol/mg protein in tet+ cell membranes. The agonist ligands 2MeSATP and alpha beta MeATP displaced the binding of 35SATP gamma S in tet- cell membranes with very high affinity, yielding pIC(50) values of 9.4+/-0.2 and 7.5+/-0. 2, respectively. In tet+ cell membrane, displacement of 35SATP gamma S by 2MeSATP and alpha beta MeATP was of much lower affinity (pIC(50) values of 7.8 and 6.2, respectively). ATP, ADP and UTP showed similar displacement of 35SATP gamma S binding in tet- and tet+ cell membranes. In other experiments, cytosolic Ca(2+) was monitored using the fluorescent indicator, fluo-3. Increases in cytosolic Ca(2+) were elicited by 100 nM alpha beta MeATP in tet- cells while no increases in cytosolic Ca(2+) were detected below 100 microM alpha beta MeATP in either tet+ cells or untransfected cells. These calcium responses to alpha beta MeATP had a pEC(50) of 6.7 and were transient, returning to baseline within 120 s. Suramin produced concentration-dependent, parallel, dextral shifts of E/A curves to alpha beta MeATP yielding a pK(B) of 5.6. PPADS produced non-parallel, dextral shifts of E/A curves to alpha beta MeATP which were insurmountable. These results show for the first time, expression of a functional, homomeric recombinant rat P2X(3) receptor which is under regulated expression in a stably transfected mammalian cell line.
How to Take a BAER, ELLEN D.; MCGOWAN, MADELINE N.; MCGIVERN, DIANE O.
The American journal of nursing,
07/1977, Letnik:
77, Številka:
7
Journal Article
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