•The overlay element method improves prediction of knit response to ballistic impact.•Knit stretchability highlights the importance of the bending response of yarns.•Simulated slip at the target's ...clamped boundary reduces stress reflection.•Methods allow a better match between simulated and experimental deflection profile.
Knit textiles constructed from continuous filament high-modulus fibers provide a unique combination of in-plane stretchability with resistance to ballistic penetration. A finite element model of ballistic impact for these materials is presented that uses beam elements overlaid on top of truss elements to capture both the tensile and bending response of the comprising knitted yarns. Accurate bending behavior, in particular, is of critical importance for knits because in-plane stretching in these materials is accommodated via bending of the multifiber yarns. Model comparisons of single layer knits impacted experimentally with spherical ballistic projectiles demonstrate improved dynamic deflection predictions compared to solid element or truss element yarn representations. Additionally, slip at the clamped fabric boundary, a common feature in ballistic experiments, is shown to be significant and is effectively represented using an elastic-plastic model at the perimeter of the simulation domain.
We measured binocular and monocular depth thresholds for objects presented in a real environment. Observers judged the depth separating a pair of metal rods presented either in relative isolation, or ...surrounded by other objects, including a textured surface. In the isolated setting, binocular thresholds were greatly superior to the monocular thresholds by as much as a factor of 18. The presence of adjacent objects and textures improved the monocular thresholds somewhat, but the superiority of binocular viewing remained substantial (roughly a factor of 10). To determine whether motion parallax would improve monocular sensitivity for the textured setting, we asked observers to move their heads laterally, so that the viewing eye was displaced by 8-10 cm; this motion produced little improvement in the monocular thresholds. We also compared disparity thresholds measured with the real rods to thresholds measured with virtual images in a standard mirror stereoscope. Surprisingly, for the two naive observers, the stereoscope thresholds were far worse than the thresholds for the real rods-a finding that indicates that stereoscope measurements for unpracticed observers should be treated with caution. With practice, the stereoscope thresholds for one observer improved to almost the precision of the thresholds for the real rods.
Summary
The x–z cross‐sectional profiles of fluorescent objects can be distorted in confocal microscopy, in large part due to mismatch between the refractive index of the immersion medium of typical ...high numerical aperture objectives and the refractive index of the medium in which the sample is present. Here, we introduce a method to mount fluorescent samples parallel to the optical axis. This mounting allows direct imaging of what would normally be an x–z cross‐section of the object, in the x–y plane of the microscope. With this approach, the x–y cross‐sections of fluorescent beads were seen to have significantly lower shape‐distortions as compared to x–z cross‐sections reconstructed from confocal z‐stacks. We further tested the method for imaging of nuclear and cellular heights in cultured cells, and found that they are significantly flatter than previously reported. This approach allows improved imaging of the x–z cross‐section of fluorescent samples.
Lay description
Optical distortions are common in confocal microscopy. In particular, the mismatch between the refractive index of the immersion medium of the microscope objective and the refractive index of the sample medium distorts the shapes of fluorescent objects in the x–z plane of the microscope. Here, we introduced a method to eliminate the shape‐distortion in the x–z cross‐sections. This was achieved by mounting fluorescent samples on vertical glass slides such that the cross‐sections orthogonal to the glass surface could be imaged in the x–y plane of the microscope. Our method successfully improved the imaging of nuclear and cellular heights in cultured cells and revealed that the heights were significantly flatter than previously reported with conventional approaches.
Amblyopia is usually defined as a deficit in optotype (Snellen) acuity with no detectable organic cause. We asked whether this visual abnormality is completely characterized by the deficit in ...optotype acuity, or whether it has distinct forms that are determined by the conditions associated with the acuity loss, such as strabismus or anisometropia. To decide this issue, we measured optotype acuity, Vernier acuity, grating acuity, contrast sensitivity, and binocular function in 427 adults with amblyopia or with risk factors for amblyopia and in a comparison group of 68 normal observers. Optotype acuity accounts for much of the variance in Vernier and grating acuity, and somewhat less of the variance in contrast sensitivity. Nevertheless, there are differences in the patterns of visual loss among the clinically defined categories, particularly between strabismic and anisometropic categories. We used factor analysis to create a succinct representation of our measurement space. This analysis revealed two main dimensions of variation in the visual performance of our abnormal sample, one related to the visual acuity measures (optotype, Vernier, and grating acuity) and the other related to the contrast sensitivity measures (Pelli-Robson and edge contrast sensitivity). Representing our data in this space reveals distinctive distributions of visual loss for different patient categories, and suggests that two consequences of the associated conditions--reduced resolution and loss of binocularity--determine the pattern of visual deficit. Non-binocular observers with mild-to-moderate acuity deficits have, on average, better monocular contrast sensitivity than do binocular observers with the same acuity loss. Despite their superior contrast sensitivity, non-binocular observers typically have poorer optotype acuity and Vernier acuity, at a given level of grating acuity, than those with residual binocular function.
The clinicopathologic characteristics of 69 cases of eccrine porocarcinoma (EP) have been studied. Seven cases of purely in situ disease are included. Forty patients were female, 29 male with ages ...ranging from 29 to 91 years (mean 73 years). The lower extremity represented the single most common site (44%). Other common sites were the trunk (15 cases, 24%) and head (11 cases, 18%). The histologic diagnosis of EP was predicated on the basis of an irregular tumor at least partly formed of characteristic poromatous basaloid epithelial cells displaying ductal differentiation, and significant cytologic atypia. Forty-seven tumors (68%) contained mature well-formed eccrine ducts having an eosinophilic luminal cuticle, with the remaining tumors containing small ill-formed ducts and/or intracytoplasmic lumina. All ducts were discernible via light microscopy and in 49 cases were highlighted with DPAS stain and/or CEA/EMA immunocytochemistry. A variant with a broad pushing tumor margin and marked nuclear pleomorphism showed some resemblance to proliferative bowenoid dysplasia. In 11 cases (18%) the tumors appeared to arise in continuity with a benign preexistent poroma. A variety of histologic patterns were displayed including clear, squamous, and spindle cell differentiation, mucus cell metaplasia, and colonization by melanocytes. Lymphovascular invasion was present in 9 cases (15%). Three cases showed pagetoid extension of malignant cells (epidermotropism) and appeared to be multifocal. Follow-up was available in 54 patients (78%) with 9 (17%) experiencing local recurrence, 10 developing lymph node metastases (19%), and 6 (11%) experiencing distant metastases or death. Mitoses, the presence of lymphovascular invasion, and tumor depth >7 mm were associated with a poorer prognosis. Dividing tumors into those with a "pushing" or "infiltrating" advancing margin was also predictive of outcome with the latter having an increased risk of local recurrence. This report, the largest series of EP to date, suggests that the incidence of aggressive behavior is less than popularly believed. Furthermore, EP can display a wide variety of histologic patterns that may lead to diagnostic error in the unwary. The large number of cases in this series enables a reliable evaluation of prognostic parameters. A more aggressive clinical course may be indicated by more than 14 mitoses per high power field (hazard ratio HR for death 17.0, 95% confidence interval CI 2.71-107), lymphovascular invasion by tumor (HR 4.41, CI 1.13-17.2), and depth >7 mm (HR 5.49, CI 1.0-30.3). Thus, mitoses, lymphovascular invasion, and tumor depth should be evaluated in these tumors. We also suggest that tumors presenting an "infiltrative" advancing margin are particularly prone to local recurrence and require wide excision with close attention to the surgical margins by the reporting pathologist.
We used source imaging of visual evoked potentials to measure neural population responses over a wide range of horizontal disparities (0.5-64 arcmin). The stimulus was a central disk that moved back ...and forth across the fixation plane at 2 Hz, surrounded either by binocularly uncorrelated dots (disparity noise) or by correlated dots presented in the fixation plane. Both disk and surround were composed of dynamic random dots to remove coherent monocular information. Disparity tuning was measured in five visual regions of interest (ROIs) V1, human middle temporal area (hMT+), V4, lateral occipital complex (LOC), and V3A, defined in separate functional magnetic resonance imaging scans. The disparity tuning functions peaked between 2 and 16 arcmin for both types of surround in each ROI. Disparity tuning in the V1 ROI was unaffected by the type of surround, but surround correlation altered both the amplitude and phase of the disparity responses in the other ROIs. Response amplitude increased when the disk was in front of the surround in the V3A and LOC ROIs, indicating that these areas encode figure-ground relationships and object convexity. The correlated surround produced a consistent phase lag at the second harmonic in the hMT+ and V4 ROIs without a change in amplitude, while in the V3A ROI, both phase and amplitude effects were observed. Sensitivity to disparity context is thus widespread in visual cortex, but the dynamics of these contextual interactions differ across regions.
Contrast sensitivity is known to be strongly influenced by the target surround, yet the role of the surround interaction in visual processing remains unclear. Previously, we have shown that the ...surround strongly suppresses contrast sensitivity in the periphery when the surround spatial frequency and orientation match those of the target (Petrov, Carandini, & McKee, 2005). Here, we explore how various spatial characteristics of the iso-oriented and frequency-matched surround, such as surround phase and spatial layout, affect suppression. We manipulated surround geometry (annulus ring, half annulus, and bow tie) and its separation from the target (both laterally and in depth) and varied the position of the half-annulus and bow-tie surrounds with respect to Gabor target's orientation and with respect to its location in the visual field (i.e., radial vs. tangential surrounds). We also compared monoptic, dichoptic, and binocular surround suppression. Except for a significant radial-tangential anisotropy, only the area of the surround and the lateral separation between the surround and target had a significant effect on the magnitude of suppression. We showed that, although suppression amplitude remains constant with stimulus eccentricity, the lateral extent of suppression scales in proportion to the eccentricity. The most surprising finding was that the extent of surround suppression does not scale with stimulus size or spatial frequency. We suggest that the properties of surround suppression are best explained by a mechanism that selects salient targets for subsequent saccades.
State updating of distributed rainfall-runoff models via streamflow assimilation is subject to overfitting because large dimensionality of the state space of the model may render the assimilation ...problem seriously under-determined. To examine the issue in the context of operational hydrologic forecasting, we carried out a set of real-world experiments in which streamflow data is assimilated into the gridded Sacramento Soil Moisture Accounting (SAC-SMA) and kinematic-wave routing models of the US National Weather Service (NWS) Research Distributed Hydrologic Model (RDHM) via variational data assimilation (DA). The nine study basins include four in Oklahoma and five in Texas. To assess the sensitivity of the performance of DA to the dimensionality of the control vector, we used nine different spatiotemporal adjustment scales, with which the state variables are adjusted in a lumped, semi-distributed, or distributed fashion and biases in precipitation and PE are adjusted at hourly or 6-hourly scale, or at the scale of the fast response of the basin. For each adjustment scale, three different assimilation scenarios were carried out in which streamflow observations are assumed to be available at basin interior points only, at the basin outlet only, or at all locations. The results for the nine basins show that the optimum spatiotemporal adjustment scale varies from basin to basin and between streamflow analysis and prediction for all three streamflow assimilation scenarios. The most preferred adjustment scale for seven out of the nine basins is found to be distributed and hourly. It was found that basins with highly correlated flows between interior and outlet locations tend to be less sensitive to the adjustment scale and could benefit more from streamflow assimilation. In comparison with outlet flow assimilation, interior flow assimilation produced streamflow predictions whose spatial correlation structure is more consistent with that of observed flow for all adjustment scales. We also describe diagnosing the complexity of the assimilation problem using spatial correlation of streamflow and discuss the effect of timing errors in hydrograph simulation on the performance of the DA procedure.
This narrative review summarizes the current knowledge of the genetic and epigenetic contributions to the development of fibromyalgia (FM). Although there is no single gene that results in the ...development of FM, this study reveals that certain polymorphisms in genes involved in the catecholaminergic pathway, the serotonergic pathway, pain processing, oxidative stress, and inflammation may influence susceptibility to FM and the severity of its symptoms. Furthermore, epigenetic changes at the DNA level may lead to the development of FM. Likewise, microRNAs may impact the expression of certain proteins that lead to the worsening of FM-associated symptoms.
The Northeast Canyons and Seamounts Marine National Monument (NEC&SMNM) was designated by President Barack Obama in 2016, using his authority under the Antiquities Act of 1906. The Act allows a ...President to proclaim as national monuments “historic landmarks, historic and prehistoric structures, and other objects of historic or scientific interest” that are “upon the lands owned or controlled” by the United States but to reserve each designation to “the smallest area compatible with the proper care and management of the objects to be protected.” Protection in general excludes commercial scale extraction and is in perpetuity. Analyses of physiographic and ecological data sets facilitated assessment of the conservation benefits of protections for the NEC&SMNM and syntheses of the ecological literature describe processes that operate in continental margin and deep-sea settings. Results indicate that the current monument designation is an area of high diversity and ecological connectivity across depths and along the continental margin. The monument boundaries contain hot spots (areas of high abundance and species richness) for seafloor communities (inclusive of benthic invertebrate and demersal fish) as well as marine mammals in the epipelagic. Many species are sensitive to disturbance and vulnerable to human activities (e.g., deep-sea corals, sponges) with very long recovery times and extremely low resilience. The monument contains at least nine exemplars of offshore northwest Atlantic marine wildlife communities and habitats (e.g., deep shelf invertebrates, shelf fish, deep sea corals and sponges in canyons and on seamounts, deep sea fish, chemosynthetic communities, deep sea soft sediment, shelf edge cetaceans & seabirds). The region is relatively undisturbed and can serve as a reference site to focus future research on ecological processes in an increasingly industrialized ocean and one subject to the synergies of regional climate effects. These results suggest that there is great potential for discovery and novel research in this first Atlantic Ocean Marine National Monument.
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