Abstract
The transition from the vegetative growth phase to flowering is a crucial checkpoint for plant reproduction and survival, especially under environmental stress conditions. Numerous factors ...regulate flowering time, including exogenous environmental cues such as day length and temperature, as well as salt and drought stresses, and endogenous phytohormone signaling cascades. Gibberellins and ABA are one classic combination of phytohormones which antagonistically regulate several biological processes, including seed dormancy and germination, primary root growth and seedling development. As regards control of flowering time, gibberellin exhibits a positive role, and represents an important pathway in the regulation of floral transition. However, over the past decades, numerous investigations have demonstrated that the contribution of the stress hormone ABA to floral transition is still controversial, as both positive and negative effects have been documented. It is important to determine why and how ABA shows this contradictory effect on flowering time. In this up to date review, primarily based on recent publications and emerging data, we summarize the distinct and contrasting roles of ABA on floral transition, while the detailed molecular mechanisms underlying these roles are discussed. Finally, the remaining challenges and open questions in this topic are presented.
Summary
Strigolactones and karrikins are butenolide molecules that regulate plant growth. They are perceived by the α/β‐hydrolase DWARF14 (D14) and its homologue KARRIKIN INSENSITIVE2 (KAI2), ...respectively. Plant‐derived strigolactones have a butenolide ring with a methyl group that is essential for bioactivity. By contrast, karrikins are abiotic in origin, and the butenolide methyl group is nonessential. KAI2 is probably a receptor for an endogenous butenolide, but the identity of this compound remains unknown.
Here we characterise the specificity of KAI2 towards differing butenolide ligands using genetic and biochemical approaches.
We find that KAI2 proteins from multiple species are most sensitive to desmethyl butenolides that lack a methyl group. Desmethyl‐GR24 and desmethyl‐CN‐debranone are active by KAI2 but not D14. They are more potent KAI2 agonists compared with their methyl‐substituted reference compounds both in vitro and in plants. The preference of KAI2 for desmethyl butenolides is conserved in Selaginella moellendorffii and Marchantia polymorpha, suggesting that it is an ancient trait in land plant evolution.
Our findings provide insight into the mechanistic basis for differential ligand perception by KAI2 and D14, and support the view that the endogenous substrates for KAI2 and D14 have distinct chemical structures and biosynthetic origins.
Soybean is an important and staple oilseed crop worldwide. Salinity stress has adverse effects on soybean development periods, especially on seed germination and post-germinative growth. Improving ...seed germination and emergence will have positive effects under salt stress conditions on agricultural production. Here we report that NaCl delays soybean seed germination by negatively regulating gibberellin (GA) while positively mediating abscisic acid (ABA) biogenesis, which leads to a decrease in the GA/ABA ratio. This study suggests that fluridone (FLUN), an ABA biogenesis inhibitor, might be a potential plant growth regulator that can promote soybean seed germination under saline stress. Different soybean cultivars, which possessed distinct genetic backgrounds, showed a similar repressed phenotype during seed germination under exogenous NaCl application. Biochemical analysis revealed that NaCl treatment led to high MDA (malondialdehyde) level during germination and the post-germinative growth stages. Furthermore, catalase, superoxide dismutase, and peroxidase activities also changed after NaCl treatment. Subsequent quantitative Real-Time Polymerase Chain Reaction analysis showed that the transcription levels of ABA and GA biogenesis and signaling genes were altered after NaCl treatment. In line with this, phytohormone measurement also revealed that NaCl considerably down-regulated active GA
, GA
, and GA
levels, whereas the ABA content was up-regulated; and therefore ratios, such as GA
/ABA, GA
/ABA, and GA
/ABA, are decreased. Consistent with the hormonal quantification, FLUN partially rescued the delayed-germination phenotype caused by NaCl-treatment. Altogether, these results demonstrate that NaCl stress inhibits soybean seed germination by decreasing the GA/ABA ratio, and that FLUN might be a potential plant growth regulator that could promote soybean seed germination under salinity stress.
Steam gasification of a typical Chinese bituminous coal for hydrogen production in a lab-scale pressurized bubbling fluidized bed with CaO as CO2 sorbent was performed over a pressure range of ...ambient pressure to 4 bar. The compositions of the product gases were analyzed and correlated to the gasification operating variables that affecting H2 production, such as pressure (P), mole ratio of steam to carbon (H2O/C), mole ratio of CaO to carbon (CaO/C) and temperature (T). The experimental results indicated that the H2 concentration was enhanced by raising the temperature, pressure and H2O/C under the circumstances we observed. With the presence of CaO sorbent, CO2 in the production gas was absorbed and converted to solid CaCO3, thus shifting the steam reforming of hydrocarbons and water gas shift reaction beyond the equilibrium restrictions and enhancing the H2 concentration. H2 concentration was up to 78 vol% (dry basis) under a condition of 750 °C, 4 bar, Ca/C = 1 and H2O/C = 2, while CO2 (2.7 vol%) was almost in-situ captured by the CaO sorbent. This study demonstrated that CaO could be used as a substantially excellent CO2 sorbent for the pressurized steam gasification of bituminous coal. For the gasification process with the presence of CaO, H2-rich syngas was yielded at far lower temperatures and pressures in comparison to the commercialized coal gasification technologies. SEM/EDX and gas sorption analyses of solid residues sampled after the gasification showed that the pore structure of the sorbent was recovered after the steam gasification process, which was attributed to the formation of Ca(OH)2. Additionally, a coal-CaO–H2O system was simulated with using Aspen Plus software. Calculation results showed that higher temperatures and pressures favor the H2 production within a certain range.
•Thermodynamic simulation of coal-CaO–H2O and coal-H2O system was performed.•A typical Chinese bituminous coal was steam gasified in a pressurized fluidized bed at a pressure of 4 bar.•H2-rich syngas production was promoted with the presence of CaO.•H2 production was enhanced with the increase of operating pressure, temperature and H2OC.•Solid residues were analyzed with SEM/EDX and XRD.
SUMMARY
KARRIKIN INSENSITIVE2 (KAI2) is an α/β‐hydrolase required for plant responses to karrikins, which are abiotic butenolides that can influence seed germination and seedling growth. Although ...represented by four angiosperm species, loss‐of‐function kai2 mutants are phenotypically inconsistent and incompletely characterised, resulting in uncertainties about the core functions of KAI2 in plant development. Here we characterised the developmental functions of KAI2 in the grass Brachypodium distachyon using molecular, physiological and biochemical approaches. Bdkai2 mutants exhibit increased internode elongation and reduced leaf chlorophyll levels, but only a modest increase in water loss from detached leaves. Bdkai2 shows increased numbers of lateral roots and reduced root hair growth, and fails to support normal root colonisation by arbuscular‐mycorrhizal (AM) fungi. The karrikins KAR1 and KAR2, and the strigolactone (SL) analogue rac‐GR24, each elicit overlapping but distinct changes to the shoot transcriptome via BdKAI2. Finally, we show that BdKAI2 exhibits a clear ligand preference for desmethyl butenolides and weak responses to methyl‐substituted SL analogues such as GR24. Our findings suggest that KAI2 has multiple roles in shoot development, root system development and transcriptional regulation in grasses. Although KAI2‐dependent AM symbiosis is likely conserved within monocots, the magnitude of the effect of KAI2 on water relations may vary across angiosperms.
Significance Statement
KAI2 is an α/β‐hydrolase receptor for abiotic karrikins and yet elusive plant hormones. The roles of KAI2 have been extensively studied in the dicotyledon Arabidopsis. Here we provide a comprehensive characterisation of the roles of KAI2 in the monocotyledon Brachypodium distachyon.
Karrikins (KAR) are a class of signal compounds, discovered in wildfire smoke, which affect seed germination. Currently, numerous studies have focused on the model plant Arabidopsis in the KAR ...research field, rather than on crops. Thus the regulatory mechanisms underlying KAR regulation of crop seed germination are largely unknown. Here, we report that KAR delayed soybean seed germination through enhancing abscisic acid (ABA) biosynthesis, while impairing gibberellin (GA) biogenesis. Interestingly, KAR only retarded soybean seed germination under shaded conditions, rather than under dark and white light conditions, which differs from in Arabidopsis. Phytohormone quantification showed that KAR enhanced ABA biogenesis while impairing GA biosynthesis during the seed imbibition process, and subsequently, the ratio of active GA4 to ABA was significantly reduced. Further qRT-PCR analysis showed that the transcription pattern of genes involved in ABA and GA metabolic pathways are consistent with the hormonal measurements. Finally, fluridone, an ABA biogenesis inhibitor, remarkably rescued the delayed-germination phenotype of KAR-treatment; and paclobutrazol, a GA biosynthesis inhibitor, inhibited soybean seed germination. Taken together, these evidences suggest that KAR inhibit soybean seed germination by mediating the ratio between GA and ABA biogenesis.
Global climate change is strongly associated with variations in precipitation and flooding events. Flooding usually causes submergence- or partial submergence stress in plants, which significantly ...has a negative influence on agricultural production, from seed germination to vegetative and reproductive growth. Flooding stress results in crop growth under low oxygen conditions and thus, negatively affects the developmental periods of plant lifecycle. The survival strategies of different plant species under this stressful condition are distinct, whereas the perception pathways associated with flooding stress are similar at the molecular level. Plants respond to flooding stress by mediating changes in their architecture, energy metabolism, photosynthesis, respiration and endogenous phytohormone biosynthesis/signaling, because aerobic respiration is inhibited under flooding stress, the decrease of energy metabolism further constrains plant development. Consequently, to acclimate under these unfavorable conditions, the anaerobic respiration cascade must be promoted. In this updated review, we primarily focus on recent advances in our understanding of the mechanisms underlying plant responses to flooding stress. We summarize the functions of the flooding response factors involved in energy metabolism and phytohormone biosynthesis/signaling cascades. Finally, the current understanding of how plants circumvent flooding stress, and the potential challenges for future research, are discussed.
•The waterlogging/flooding stress influence the plant growth and development.•The review reveal the plant response to waterlogging/flooding stress throughout life-cycle.•Waterlogging/flooding stress influence the plant photosynthesis and energy metabolism.•Phytohormone-mediated pathways involved in plant waterlogging stress.
Auxin is an important phytohormone which mediates diverse development processes in plants. Published research has demonstrated that auxin induces seed dormancy. However, the precise mechanisms ...underlying the effect of auxin on seed germination need further investigation, especially the relationship between auxins and both abscisic acid (ABA) and gibberellins (GAs), the latter two phytohormones being the key regulators of seed germination. Here we report that exogenous auxin treatment represses soybean seed germination by enhancing ABA biosynthesis, while impairing GA biogenesis, and finally decreasing GA
/ABA and GA
/ABA ratios. Microscope observation showed that auxin treatment delayed rupture of the soybean seed coat and radicle protrusion. qPCR assay revealed that transcription of the genes involved in ABA biosynthetic pathway was up-regulated by application of auxin, while expression of genes involved in GA biosynthetic pathway was down-regulated. Accordingly, further phytohormone quantification shows that auxin significantly increased ABA content, whereas the active GA
and GA
levels were decreased, resulting insignificant decreases in the ratiosGA
/ABA and GA
/ABA.Consistent with this, ABA biosynthesis inhibitor fluridone reversed the delayed-germination phenotype associated with auxin treatment, while paclobutrazol, a GA biosynthesis inhibitor, inhibited soybean seed germination. Altogether, exogenous auxin represses soybean seed germination by mediating ABA and GA biosynthesis.
The bolt-steel plate connection stands as a dry connection method in precast structures characterized by its simplicity, high efficiency, ease of assembly and disassembly, and environmental ...protection. Five precast concrete sandwich walls (PCSWs) with glass fiber-reinforced polymer (GFRP) to connect the interior and exterior concrete wythes were designed and tested to investigate the seismic performance of such walls under cyclic loads. Ultra-high performance concrete (UHPC) was used to strengthen the bolt-steel plate connection joint. This study investigated the effect of the sandwich wall between the two bolt-steel plate connection joints, opening, and UHPC on the seismic performance of PCSWs. The findings revealed that the sandwich wall between the two bolt-steel plate connection joints, UHPC, and the interface between UHPC and ordinary concrete had a significant influence on the failure modes, including four failure modes: shear failure of bolt-steel plate connection joint, concrete crushing at the wall toe and through-crack of the shear oblique cracks and horizontal joint, shear failure at the interface between the solid and sandwich walls, and shear failure typified by a "Keyway" configuration. The use of UHPC to strengthen the bolt-steel plate connection area could significantly improve the load-bearing capacity and peak drift ratio. Whether the sandwich wall was used in the non-bolt-steel plate connection area in the horizontal joint zone had minimal influence on both the load-bearing capacity and peak drift ratio. For the walls without UHPC strengthening, the ultimate load and ultimate drift ratio could be increased by using the sandwich wall between the two bolt-steel plate connection areas, however, it was the opposite when UHPC strengthening was used. The paper also compared the sandwich walls with the solid walls, and the results showed that the sandwich wall with bolt-steel plate connections had good seismic performance.
•A bolt-steel plate connection precast concrete sandwich wall with glass fiber-reinforced polymer (GFRP) was presented.•Tests were conducted to study the seismic performance of the bolt-steel plate connection precast concrete sandwich wall.•Comparative analysis of the sandwich and solid walls with bolt connections.
The plant growth regulator diethyl aminoethyl hexanoate (DA-6) promotes the germination and seedling establishment of aged soybean seeds through enhancing the conversion from triacylglycerol to fatty ...acids and sugars.
Abstract
Soybean seeds contain higher concentrations of oil (triacylglycerol) and fatty acids than do cereal crop seeds, and the oxidation of these biomolecules during seed storage significantly shortens seed longevity and decreases germination ability. Here, we report that diethyl aminoethyl hexanoate (DA-6), a plant growth regulator, increases germination and seedling establishment from aged soybean seeds by increasing fatty acid metabolism and glycometabolism. Phenotypic analysis showed that DA-6 treatment markedly promoted germination and seedling establishment from naturally and artificially aged soybean seeds. Further analysis revealed that DA-6 increased the concentrations of soluble sugars during imbibition of aged soybean seeds. Consistently, the concentrations of several different fatty acids in DA-6-treated aged seeds were higher than those in untreated aged seeds. Subsequently, quantitative PCR analysis indicated that DA-6 induced the transcription of several key genes involved in the hydrolysis of triacylglycerol to sugars in aged soybean seeds. Furthermore, the activity of invertase in aged seeds, which catalyzes the hydrolysis of sucrose to form fructose and glucose, increased following DA-6 treatment. Taken together, DA-6 promotes germination and seedling establishment from aged soybean seeds by enhancing the hydrolysis of triacylglycerol and the conversion of fatty acids to sugars.
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