Semi-arid woodlands, which are characterised by patchy vegetation interspersed with bare, open areas, are frequently exposed to wildfire. During summer, long dry periods are occasionally interrupted ...by rainfall events. It is well known that rewetting of dry soil induces a flush of respiration. However, the magnitude of the flush may differ between vegetation patches and open areas because of different organic matter content, which could be further modulated by wildfire. Soils were collected from under trees, under shrubs or in open areas in unburnt and burnt sandy mallee woodland, where part of the woodland experienced a wildfire which destroyed or damaged most of the aboveground plant parts 4 months before sampling. In an incubation experiment, the soils were exposed to two moisture treatments: constantly moist (CM) and drying and rewetting (DRW). In CM, soils were incubated at 80 % of maximum water holding capacity (WHC) for 19 days; in DRW, soils were dried for 4 days, kept dry for another 5 days, then rewetted to 80 % WHC and maintained at this water content until day 19. Soil respiration decreased during drying and was very low in the dry period; rewetting induced a respiration flush. Compared to soil under shrubs and in open areas, cumulative respiration per gram of soil in CM and DRW was greater under trees, but lower when expressed per gram of total organic carbon (TOC). Organic matter content, available P, and microbial biomass C, but not available N, were greater under trees than in open areas. Wild fire decreased the flush of respiration per gram of TOC in the open areas and under shrubs, and reduced TOC and microbial biomass C (MBC) concentrations only under trees, but had little effect on available N and P concentrations. We conclude that the impact of wildfire and DRW events on nutrient cycling differs among vegetation patches of a native semi-arid woodland which is related to organic matter amount and availability.
Spinal cord stimulation (SCS) is a useful neuromodulatory technique for treatment of certain neuropathic pain conditions. However, the optimal stimulation parameters remain unclear.
In rats after L5 ...spinal nerve ligation, the authors compared the inhibitory effects on mechanical hypersensitivity from bipolar SCS of different intensities (20, 40, and 80% motor threshold) and frequencies (50, 1 kHz, and 10 kHz). The authors then compared the effects of 1 and 50 Hz dorsal column stimulation at high- and low-stimulus intensities on conduction properties of afferent Aα/β-fibers and spinal wide-dynamic-range neuronal excitability.
Three consecutive daily SCS at different frequencies progressively inhibited mechanical hypersensitivity in an intensity-dependent manner. At 80% motor threshold, the ipsilateral paw withdrawal threshold (% preinjury) increased significantly from pre-SCS measures, beginning with the first day of SCS at the frequencies of 1 kHz (50.2 ± 5.7% from 23.9 ± 2.6%, n = 19, mean ± SEM) and 10 kHz (50.8 ± 4.4% from 27.9 ± 2.3%, n = 17), whereas it was significantly increased beginning on the second day in the 50 Hz group (38.9 ± 4.6% from 23.8 ± 2.1%, n = 17). At high intensity, both 1 and 50 Hz dorsal column stimulation reduced Aα/β-compound action potential size recorded at the sciatic nerve, but only 1 kHz stimulation was partially effective at the lower intensity. The number of actions potentials in C-fiber component of wide-dynamic-range neuronal response to windup-inducing stimulation was significantly decreased after 50 Hz (147.4 ± 23.6 from 228.1 ± 39.0, n = 13), but not 1 kHz (n = 15), dorsal column stimulation.
Kilohertz SCS attenuated mechanical hypersensitivity in a time course and amplitude that differed from conventional 50 Hz SCS, and may involve different peripheral and spinal segmental mechanisms.
Infections are associated with morbimortality of patients with giant cell arteritis (GCA). The aim of this work was twofold: the identification of factors predisposing to the risk of infection and ...the description of patients hospitalized with an infection occurring during the treatment period of CAG.
A monocentric retrospective study was conducted in GCA patients, comparing patients hospitalized for infection with patients without infection. The analysis included 21/144 (14.6%) patients with 26 infections (cases) and 42 control matched on sex, age, and diagnosis of GCA.
Both groups were similar except for a higher frequency of seritis in cases (15% vs. 0%, p=0.03). Relapses of GCA were less common in cases (23.8% vs 50.0%, p=0.041). Hypogammaglobulinemia was present during infection. More than half of the infections (53.8%) occurred in the first year of follow-up with an average dose of 15mg/day of corticosteroids. Infections were mainly pulmonary (46.2%) and cutaneous (26.9%).
Factors associated with infectious risk were identified. This preliminary monocentric work will continue with a national multicentre study.
Serum lipids, diabetes, and obesity, individual components of metabolic syndrome, are associated with biliary tract cancer and stone risk, but the associations of metabolic syndrome or insulin ...resistance with biliary tract cancers and stones are not well studied.
In this population-based case-control study in Shanghai, China (627 biliary tract cancers, 1037 biliary stones, and 959 controls), metabolic syndrome was defined as the presence of any three of the five components, including high waist circumference, high triglycerides, low high-density lipoprotein cholesterol (HDL), high blood pressure, and diabetes. Insulin resistance and β-cell function were assessed, using homeostasis assessment models.
Metabolic syndrome was significantly associated with gallbladder cancer (odds ratio (OR)=2.75, 95% confidence interval (95% CI)=1.82-4.15) and biliary stones (OR=1.64, 95% CI=1.24-2.16), with a significant dose effect with increasing number of metabolic syndrome components (P trend <0.0001). The observed association persisted among subjects without a history of diabetes. The association between insulin resistance and gallbladder cancer was borderline (P trend=0.06). There was a significant inverse association between β-cell function and gallbladder cancer risk (P trend <0.001).
Our findings suggest that metabolic syndrome and insulin resistance have a role in the aetiology of biliary tract cancers and biliary stones, and if confirmed, they imply that lifestyle control of these factors may lower the risk of biliary stones and biliary tract cancer.
Antimicrobial resistance (AMR) is a concern as this increases morbidity, mortality, and costs, with sub-Saharan Africa having the highest rates globally. Concerns with rising AMR have resulted in ...international, Pan-African, and country activities including the development of national action plans (NAPs). However, there is variable implementation across Africa with key challenges persisting.
Consequently, there is an urgent need to document current NAP activities and challenges across sub-Saharan Africa to provide future guidance. This builds on a narrative review of the literature.
All surveyed sub-Saharan African countries have developed their NAPs; however, there is variable implementation. Countries including Botswana and Namibia are yet to officially launch their NAPs with Eswatini only recently launching its NAP. Cameroon is further ahead with its NAP than these countries; though there are concerns with implementation. South Africa appears to have made the greatest strides with implementing its NAP including regular monitoring of activities and instigation of antimicrobial stewardship programs. Key challenges remain across Africa. These include available personnel, expertise, capacity, and resources to undertake agreed NAP activities including active surveillance, lack of focal points to drive NAPs, and competing demands and priorities including among donors. These challenges are being addressed, with further co-ordinated efforts needed to reduce AMR.
SUMMARY
Earth observation satellites yield a wealth of data for scientific, operational and commercial exploitation. However, the redistribution of mass in the system Earth is not yet part of the ...standard inventory of Earth Observation (EO) data products to date. It is derived from the Gravity Recovery and Climate Experiment (GRACE) mission and its Follow-On mission (GRACE-FO). Among many other applications, mass redistribution provides fundamental insights into the global water cycle. Changes in continental water storage impact the regional water budget and can, in extreme cases, result in floods and droughts that often claim a high toll on infrastructure, economy and human lives. The initiative for a European Gravity Service for Improved Emergency Management (EGSIEM) established three different prototype services to promote the unique value of mass redistribution products for Earth Observation in general and for early-warning systems in particular. The first prototype service is a scientific combination service to derive improved mass redistribution products from the combined knowledge of the European GRACE analysis centres. Second, the timeliness and reliability of such products is a primary concern for any early-warning system and therefore EGSIEM established a prototype for a near real-time service that provides dedicated gravity field information with a maximum latency of 5 d. Third, EGSIEM established a prototype of a hydrological/early warning service that derives wetness indices as indicators of hydrological extremes and assessed their potential for timely scheduling of high-resolution optical/radar satellites for follow-up observations in case of evolving hydrological extreme events.
The purpose of this study was to investigate neuronal and vascular functional deficits in the retina and their association in a diabetic mouse model. We measured electroretinography (ERG) responses ...and choroidal and retinal blood flow (ChBF, RBF) with magnetic resonance imaging (MRI) in healthy and diabetic mice under basal conditions and under hypercapnic challenge.
Ins2Akita diabetic (Diab, n = 8) and age-matched, wild-type C57BL/6J mice (Ctrl, n = 8) were studied under room air and moderate hypercapnia (5% CO2). Dark-adapted ERG a-wave, b-wave, and oscillatory potentials (OPs) were measured for a series of flashes. Regional ChBF and RBF under air and hypercapnia were measured using MRI in the same mice.
Under room air, Diab mice had compromised ERG b-wave and OPs (e.g., b-wave amplitude was 422.2±10.7 μV in Diab vs. 600.1±13.9 μV in Ctrl, p < 0.001). Under hypercapnia, OPs and b-wave amplitudes were significantly reduced in Diab (OPs by 30.3±3.0% in Diab vs. -3.0±3.6% in Ctrl, b-wave by 17.9±1.4% in Diab vs. 1.3±0.5% in Ctrl). Both ChBF and RBF had significant differences in regional blood flow, with Diab mice having substantially lower blood flow in the nasal region (ChBF was 5.4±1.0 ml/g/min in Diab vs. 8.6±1.0 ml/g/min in Ctrl, RBF was 0.91±0.10 ml/g/min in Diab vs. 1.52±0.24 ml/g/min in Ctrl). Under hypercapnia, ChBF increased in both Ctrl and Diab without significant group difference (31±7% in Diab vs. 17±7% in Ctrl, p > 0.05), but an increase in RBF was not detected for either group.
Inner retinal neuronal function and both retinal and choroidal blood flow were impaired in Diab mice. Hypercapnia further compromised inner retinal neuronal function in diabetes, while the blood flow response was not affected, suggesting that the diabetic retina has difficulty adapting to metabolic challenges due to factors other than impaired blood flow regulation.
Due to their abundance in the blood, low RNA content, and short lifespan, neutrophils have been classically considered to be one homogenous pool. However, recent work has found that mature ...neutrophils and neutrophil progenitors are composed of unique subsets exhibiting context-dependent functions. In this study, we ask if neutrophil heterogeneity is associated with melanoma incidence and/or disease stage.
Using mass cytometry, we profiled melanoma patient blood for unique cell surface markers among neutrophils. Markers were tested for their predictiveness using flow cytometry data and random forest machine learning.
We identified CD79b
neutrophils (CD3
CD56
CD19
Siglec8
CD203c
CD86
CD66b
CD79b
) that are normally restricted to the bone marrow in healthy humans but appear in the blood of subjects with early-stage melanoma. Further, we found CD79b
neutrophils present in tumors of subjects with head and neck cancer. AI-mediated machine learning analysis of neutrophils from subjects with melanoma confirmed that CD79b expression among peripheral blood neutrophils is highly important in identifying melanoma incidence. We noted that CD79b
neutrophils possessed a neutrophilic appearance but have transcriptional and surface-marker phenotypes reminiscent of B cells. Compared to remaining blood neutrophils, CD79b
neutrophils are primed for NETosis, express higher levels of antigen presentation-related proteins, and have an increased capacity for phagocytosis.
Our work suggests that CD79b
neutrophils are associated with early-stage melanoma.
A search for neutral heavy resonances is performed in the
W
W
→
e
ν
μ
ν
decay channel using
pp
collision data corresponding to an integrated luminosity of
36.1
fb
-
1
, collected at a centre-of-mass ...energy of 13
TeV
by the ATLAS detector at the Large Hadron Collider. No evidence of such heavy resonances is found. In the search for production via the quark–antiquark annihilation or gluon–gluon fusion process, upper limits on
σ
X
×
B
(
X
→
W
W
)
as a function of the resonance mass are obtained in the mass range between 200
GeV
and up to 5
TeV
for various benchmark models: a Higgs-like scalar in different width scenarios, a two-Higgs-doublet model, a heavy vector triplet model, and a warped extra dimensions model. In the vector-boson fusion process, constraints are also obtained on these resonances, as well as on a Higgs boson in the Georgi–Machacek model and a heavy tensor particle coupling only to gauge bosons.
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