Antifungal activity of new copper(II) complexes of 2-methylthionicotinate (2-MeSNic) of the composition Cu(2-MeSNic)2(MeNia)(2).4H2O (where MeNia is N-methylnicotinamide), and ...Cu(2-MeSNic)2(Nia)(2).2H2O (where Nia is nicotinamide) and Cu(2-MeSNic)2L2 (where L is isonicotinamide, iNia, or ethyl nicotinate, EtNic) were tested on various strains of filamentous fungi by the macrodilution method. Most sensitive against copper(II) adducts with bioactive ligands were Rhizopus oryzae and Microsporum gypseum (IC50 1.5-2.3 mmol/L). The adducts with Nia, MeNia and EtNic at 5 mmol/L induced morphological changes in growing hyphae of Botrytis cinerea, mainly their intensive branching attached to release of cytoplasm with partial growth inhibition. Inhibition of sporulation (> 90%) of Alternaria alternata by Cu(2-MeSNic)2.H2O was observed as a change in the color of the colonies. The highest resistance was marked by B. cinerea and Fusarium moniliforme (average IC50 values 4.25 and 3.13 mmol/L, respectively). The presence of all bioactive ligands in copper(II) complexes caused an increase in the inhibition effect against model fungi (except significant inhibition activity of EtNic on R. oryzae).
Antifungal activity of new copper(II) complexes of 2-methylthionicotinate (2-MeSNic) of the composition Cu(2-MeSNic)2(MeNia)2·4H2O (where MeNia isN-methylnicotinamide), and Cu(2-MeSNic)2(Nia)2·2H2O ...(where Nia is nicotinamide) and Cu(2-MeSNic)2L2 (where L is isonicotinamide, iNia, or ethyl nicotinate, EtNic) were tested on various strains of filamentous fungi by the macrodilution method. Most sensitive against copper(II) adducts with bioactive ligands wereRhizopus oryzae andMicrosporum gypseum (IC50 1.5–2.3 mmol/L). The adducts with Nia, MeNia and EtNic at 5 mmol/L induced morphological changes in growing hyphae ofBotrytis cinerea, mainly their intensive branching attached to release of cytoplasm with partial growth inhibition. Inhibition of sporulation (>90%) ofAlternaria alternata by Cu(2-MeSNic)2·H2O was observed as a change in the color of the colonies. The highest resistance was marked byB. cinerea andFusarium moniliforme (average IC50 values 4.25 and 3.13 mmol/L, respectively). The presence of all bioactive ligands in copper(II) complexes caused an increase in the inhibition effect against model fungi (except significant inhibition activity of EtNic onR. oryzae).
226 wild mice originating from 16 different localities were tested serologically, using a battery of anti-H-2 sera. The results indicate the existence of a large number of hitherto unknown H-2 ...haplotypes. Three cogenic resistant strains (B10.W44, B10.W67, B10.W625) were prepared, their H-2 haplotypes being derived from wild mice. The H-2 haplotypes of these strains are different from the haplotypes of the existing inbred mouse strains but they share with them some public H-2 antigenic specificities. An analysis of the antisera prepared against H-2wild haplotypes allowed the detection of the private antigens, H-2.107 (B10.W44), H-2.108 (B10.W67), and H-2.109 (B10. W625). Furthermore, new public antigenic specificities, H-2.60, 61, 62, were defined. All the three H-2wild haplotypes possess the Ss-high allele. Blood cells from many wild mice as well as from the individuals of the new CR strains gave positive reactions with some antisera containing antibodies against the private specificities of H-2 haplotypes of the inbred mouse strains. However, these reactions were only the consequence of the cross-reactions of anti-H-2 inbred sera with antigenic products of wild mice. In the set of wild mice tested, a frequent occurrence of the same H-2 phenotype in individuals originating from the same locality could not be confirmed.
The role of H-2 and non-H-2 gene products was studied in a model of non-immune cell-cell interactions which underlie the homing affinity to lymph nodes of injected 51Cr-labelled lymph node cells. The ...effect of various donor-recipient combinations was tested by comparing the allogeneic/syngeneic ratio of radioactivity recovered from the lymph nodes. The homing affinity was reduced to about 50% when the donor-recipient incompatibility extended over the whole H-2 chromosome. When confined to a single region (H-2K, I or D) H-2 incompatibility caused no significant allogeneic inhibition of the homing affinity. The cumulative effect of two partial incompatibilities (K + I or D + I) was, however, reflected in a significant allogeneic inhibition. Non-H-2 incompatibilities had, as a rule, a weak effect; the non-H-2 gene products may not be directly involved in the cell interactions under test.
