Form, development and function of grass stomata Nunes, Tiago D. G.; Zhang, Dan; Raissig, Michael T.
The Plant journal : for cell and molecular biology,
February 2020, Letnik:
101, Številka:
4
Journal Article
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Summary
Stomata are cellular breathing pores on leaves that open and close to absorb photosynthetic carbon dioxide and to restrict water loss through transpiration, respectively. Grasses (Poaceae) ...form morphologically innovative stomata, which consist of two dumbbell‐shaped guard cells flanked by two lateral subsidiary cells (SCs). This ‘graminoid’ morphology is associated with faster stomatal movements leading to more water‐efficient gas exchange in changing environments. Here, we offer a genetic and mechanistic perspective on the unique graminoid form of grass stomata and the developmental innovations during stomatal cell lineage initiation, recruitment of SCs and stomatal morphogenesis. Furthermore, the functional consequences of the four‐celled, graminoid stomatal morphology are summarized. We compile the identified players relevant for stomatal opening and closing in grasses, and discuss possible mechanisms leading to cell‐type‐specific regulation of osmotic potential and turgor. In conclusion, we propose that the investigation of functionally superior grass stomata might reveal routes to improve water‐stress resilience of agriculturally relevant plants in a changing climate.
Significance Statement
A key challenge for plants is to efficiently use water, particularly when growing in a hot and dry climate. Stomata – cellular breathing pores on leaves that mediate gas exchange between plant and atmosphere – have a pivotal role in controlling water‐use efficiency. Stomata can adjust their pore size to balance carbon dioxide uptake with water vapour loss. Interestingly, grasses like the three most important food crops rice, maize and wheat have improved stomata that can regulate water use more efficiently by opening and closing faster than other plants. Rapid stomatal movements are linked to the grass stomata's unique morphology consisting of dumbbell‐shaped guard cells and lateral subsidiary or helper cells. Recently, work on domesticated and wild grass species has started to reveal some of the secrets regarding how grass stomata form and function more efficiently.
The global acidification of the earth's oceans is predicted to impact biodiversity via physiological effects impacting growth, survival, reproduction, and immunology, leading to changes in species ...abundances and global distributions. However, the degree to which these changes will play out critically depends on the evolutionary rate at which populations will respond to natural selection imposed by ocean acidification, which remains largely unquantified. Here we measure the potential for an evolutionary response to ocean acidification in larval development rate in two coastal invertebrates using a full-factorial breeding design. We show that the sea urchin species Strongylocentrotus franciscanus has vastly greater levels of phenotypic and genetic variation for larval size in future CO(2) conditions compared to the mussel species Mytilus trossulus. Using these measures we demonstrate that S. franciscanus may have faster evolutionary responses within 50 years of the onset of predicted year-2100 CO(2) conditions despite having lower population turnover rates. Our comparisons suggest that information on genetic variation, phenotypic variation, and key demographic parameters, may lend valuable insight into relative evolutionary potentials across a large number of species.
Sharp Alfvénic Impulses in the Near-Sun Solar Wind Horbury, Timothy S.; Woolley, Thomas; Laker, Ronan ...
The Astrophysical journal. Supplement series,
02/2020, Letnik:
246, Številka:
2
Journal Article
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Measurements of the near-Sun solar wind by the Parker Solar Probe have revealed the presence of large numbers of discrete Alfvénic impulses with an anti-sunward sense of propagation. These are ...similar to those previously observed near 1 au, in high speed streams over the Sun's poles and at 60 solar radii. At 35 solar radii, however, they are typically shorter and sharper than seen elsewhere. In addition, these spikes occur in "patches" and there are also clear periods within the same stream when they do not occur; the timescale of these patches might be related to the rate at which the spacecraft magnetic footpoint tracks across the coronal hole from which the plasma originated. While the velocity fluctuations associated with these spikes are typically under 100 km s−1, due to the rather low Alfvén speeds in the streams observed by the spacecraft to date, these are still associated with large angular deflections of the magnetic field-and these deflections are not isotropic. These deflections do not appear to be related to the recently reported large-scale, pro-rotation solar wind flow. Estimates of the size and shape of the spikes reveal high aspect ratio flow-aligned structures with a transverse scale of 104 km. These events might be signatures of near-Sun impulsive reconnection events.
The prediction of a supersonic solar wind
was first confirmed by spacecraft near Earth
and later by spacecraft at heliocentric distances as small as 62 solar radii
. These missions showed that plasma ...accelerates as it emerges from the corona, aided by unidentified processes that transport energy outwards from the Sun before depositing it in the wind. Alfvénic fluctuations are a promising candidate for such a process because they are seen in the corona and solar wind and contain considerable energy
. Magnetic tension forces the corona to co-rotate with the Sun, but any residual rotation far from the Sun reported until now has been much smaller than the amplitude of waves and deflections from interacting wind streams
. Here we report observations of solar-wind plasma at heliocentric distances of about 35 solar radii
, well within the distance at which stream interactions become important. We find that Alfvén waves organize into structured velocity spikes with duration of up to minutes, which are associated with propagating S-like bends in the magnetic-field lines. We detect an increasing rotational component to the flow velocity of the solar wind around the Sun, peaking at 35 to 50 kilometres per second-considerably above the amplitude of the waves. These flows exceed classical velocity predictions of a few kilometres per second, challenging models of circulation in the corona and calling into question our understanding of how stars lose angular momentum and spin down as they age
.
Quantum materials are amenable to nonequilibrium manipulation with light, enabling modification and control of macroscopic properties. Light-based augmentation of superconductivity is particularly ...intriguing. Copper-oxide superconductors exhibit complex interplay between spin order, charge order, and superconductivity, offering the prospect of enhanced coherence by altering the balance between competing orders. We utilize terahertz time-domain spectroscopy to monitor the c-axis Josephson plasma resonance (JPR) in La2−xBaxCuO4 (x = 0.115) as a direct probe of superconductivity dynamics following excitation with near-infrared pulses. Starting from the superconducting state, c-axis polarized excitation with a fluence of 100 μJ/cm2 results in an increase of the far-infrared spectral weight by more than an order of magnitude as evidenced by a blueshift of the JPR, interpreted as resulting from nonthermal collapse of the charge order. The photoinduced signal persists well beyond our measurement window of 300 ps and exhibits signatures of spatial inhomogeneity. The electrodynamic response of this metastable state is consistent with enhanced superconducting fluctuations. Our results reveal that La2−xBaxCuO4 is highly sensitive to nonequilibrium excitation over a wide fluence range, providing an unambiguous example of photoinduced modification of order-parameter competition.
The dissipation of magnetized turbulence is an important paradigm for describing heating and energy transfer in astrophysical environments such as the solar corona and wind; however, the specific ...collisionless processes behind dissipation and heating remain relatively unconstrained by measurements. Remote sensing observations have suggested the presence of strong temperature anisotropy in the solar corona consistent with cyclotron resonant heating. In the solar wind, in situ magnetic field measurements reveal the presence of cyclotron waves, while measured ion velocity distribution functions have hinted at the active presence of cyclotron resonance. Here, we present Parker Solar Probe observations that connect the presence of ion-cyclotron waves directly to signatures of resonant damping in observed proton-velocity distributions using the framework of quasilinear theory. We show that the quasilinear evolution of the observed distribution functions should absorb the observed cyclotron wave population with a heating rate of 10^{-14} W/m^{3}, indicating significant heating of the solar wind.
Over the past 75 years, human pathogens have acquired antibiotic resistance genes (ARGs), often from environmental bacteria. Integrons play a major role in the acquisition of antibiotic resistance ...genes. We therefore hypothesized that focused exploration of integron gene cassettes from microbial communities could be an efficient way to find novel mobile resistance genes. DNA from polluted Indian river sediments were amplified using three sets of primers targeting class 1 integrons and sequenced by long- and short-read technologies to maintain both accuracy and context.
Up to 89% of identified open reading frames encode known resistance genes, or variations thereof (> 1000). We identified putative novel ARGs to aminoglycosides, beta-lactams, trimethoprim, rifampicin, and chloramphenicol, including several novel OXA variants, providing reduced susceptibility to carbapenems. One dihydropteroate synthase gene, with less than 34% amino acid identity to the three known mobile sulfonamide resistance genes (sul1-3), provided complete resistance when expressed in Escherichia coli. The mobilized gene, here named sul4, is the first mobile sulfonamide resistance gene discovered since 2003. Analyses of adjacent DNA suggest that sul4 has been decontextualized from a set of chromosomal genes involved in folate synthesis in its original host, likely within the phylum Chloroflexi. The presence of an insertion sequence common region element could provide mobility to the entire integron. Screening of 6489 metagenomic datasets revealed that sul4 is already widespread in seven countries across Asia and Europe.
Our findings show that exploring integrons from environmental communities with a history of antibiotic exposure can provide an efficient way to find novel, mobile resistance genes. The mobilization of a fourth sulfonamide resistance gene is likely to provide expanded opportunities for sulfonamide resistance to spread, with potential impacts on both human and animal health.
Although normothermic machine perfusion of donor livers may allow assessment of graft viability prior to transplantation, there are currently no data on what would be a good parameter of graft ...viability. To determine whether bile production is a suitable biomarker that can be used to discriminate viable from non-viable livers we have studied functional performance as well as biochemical and histological evidence of hepatobiliary injury during ex vivo normothermic machine perfusion of human donor livers. After a median duration of cold storage of 6.5 h, twelve extended criteria human donor livers that were declined for transplantation were ex vivo perfused for 6 h at 37 °C with an oxygenated solution based on red blood cells and plasma, using pressure controlled pulsatile perfusion of the hepatic artery and continuous portal perfusion. During perfusion, two patterns of bile flow were identified: (1) steadily increasing bile production, resulting in a cumulative output of ≥ 30 g after 6 h (high bile output group), and (2) a cumulative bile production <20 g in 6 h (low bile output group). Concentrations of transaminases and potassium in the perfusion fluid were significantly higher in the low bile output group, compared to the high bile output group. Biliary concentrations of bilirubin and bicarbonate were respectively 4 times and 2 times higher in the high bile output group. Livers in the low bile output group displayed more signs of hepatic necrosis and venous congestion, compared to the high bile output group. In conclusion, bile production could be an easily assessable biomarker of hepatic viability during ex vivo machine perfusion of human donor livers. It could potentially be used to identify extended criteria livers that are suitable for transplantation. These ex vivo findings need to be confirmed in a transplant experiment or a clinical trial.
High-resolution time-of-flight measurements of H atom products from photolysis of phenol, 4-methylphenol, 4-fluorophenol, and thiophenol, at many UV wavelengths (λphot), have allowed systematic study ...of the influence of ring substituents and the heteroatom on the fragmentation dynamics. All dissociate by XFormula H (X = O, S) bond fission after excitation at their respective S₁(¹ππ*)-S₀ origins and at all shorter wavelengths. The achieved kinetic energy resolution reveals population of selected vibrational levels of the various phenoxyl and thiophenoxyl coproducts, providing uniquely detailed insights into the fragmentation dynamics. Dissociation in all cases is deduced to involve nuclear motion on the ¹πσ* potential energy surface (PES). The route to accessing this PES, and the subsequent dynamics, is seen to be very sensitive to λphot and substitution of the heteroatom. In the case of the phenols, dissociation after excitation at long λphot is rationalized in terms of radiationless transfer from S₁ to S₀ levels carrying sufficient OFormula H stretch vibrational energy to allow coupling via the conical intersection between the S₀ and ¹πσ* PESs at longer OFormula H bond lengths. In contrast, H + C₆H₅O(X²B₁) products formed after excitation at short λphot exhibit anisotropic recoil-velocity distributions, consistent with prompt dissociation induced by coupling between the photoprepared ¹ππ* excited state and the ¹πσ* PES. The fragmentation dynamics of thiophenol at all λphot matches the latter behavior more closely, reflecting the different relative dispositions of the ¹ππ* and ¹πσ* PESs. Additional insights are provided by the observed branching into the ground (X²B₁) and first excited (²B₂) states of the resulting C₆H₅S radicals.
In a single-center phase 1-2a study, the anti-CD19 chimeric antigen receptor (CAR) T-cell therapy tisagenlecleucel produced high rates of complete remission and was associated with serious but mainly ...reversible toxic effects in children and young adults with relapsed or refractory B-cell acute lymphoblastic leukemia (ALL).
We conducted a phase 2, single-cohort, 25-center, global study of tisagenlecleucel in pediatric and young adult patients with CD19+ relapsed or refractory B-cell ALL. The primary end point was the overall remission rate (the rate of complete remission or complete remission with incomplete hematologic recovery) within 3 months.
For this planned analysis, 75 patients received an infusion of tisagenlecleucel and could be evaluated for efficacy. The overall remission rate within 3 months was 81%, with all patients who had a response to treatment found to be negative for minimal residual disease, as assessed by means of flow cytometry. The rates of event-free survival and overall survival were 73% (95% confidence interval CI, 60 to 82) and 90% (95% CI, 81 to 95), respectively, at 6 months and 50% (95% CI, 35 to 64) and 76% (95% CI, 63 to 86) at 12 months. The median duration of remission was not reached. Persistence of tisagenlecleucel in the blood was observed for as long as 20 months. Grade 3 or 4 adverse events that were suspected to be related to tisagenlecleucel occurred in 73% of patients. The cytokine release syndrome occurred in 77% of patients, 48% of whom received tocilizumab. Neurologic events occurred in 40% of patients and were managed with supportive care, and no cerebral edema was reported.
In this global study of CAR T-cell therapy, a single infusion of tisagenlecleucel provided durable remission with long-term persistence in pediatric and young adult patients with relapsed or refractory B-cell ALL, with transient high-grade toxic effects. (Funded by Novartis Pharmaceuticals; ClinicalTrials.gov number, NCT02435849 .).
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