The development of a meat-based food, with a pasty texture, meets the need to improve the nutritional intake (mainly protein), in children diagnosed with cerebral palsy and dysphagia disorders. This ...study evaluated the use of oregano essential oil (EO) on the development of an infant food (IF) for children with cerebral palsy and dysphagia disorders, and the shelf-life (polyphenols content, antioxidant activity, lipid oxidation, color, pH, syneresis and adhesives) of the product during 28 days of storage. Four treatments were developed: Standard IF (STD), without EO; Control IF (CON), with BHT; IF with 0.01% EO (EO-0.01%), and IF with 0.05% EO (EO-0.05%). The results indicated that samples containing oregano EO presented higher antioxidant activity (p < 0.01) than CON and STD. Also, EO-0.05% presented the highest polyphenol content (p < 0.02). All samples showed an increase in malonaldehyde value during storage (p < 0.001), however, this increase was more accelerated for the STD and CON. Also, STD, CON and EO-0.01% showed a reduction in syneresis and all treatments showed a reduction in adhesiveness during storage. Sample EO-0.05% showed better results in most of the analyses compared to the other treatments, demonstrating that it is possible to use a natural compound in the development of this product.
•Effect of oregano essential oil and BHT on physical-chemical attributes of infant food.•Oregano essential oils and BHT presented lower lipid oxidation after 28 days.•Oregano essential oil decreased color losses, syneresis and maintained pH.
The use of tilapia waste in the production of mortadella can add value to the Nile tilapia production chain. Aiming to develop and characterize mortadella from filleting residues, two experiments ...were conducted: (1) in a 2 × 3 factorial design, using two types of raw material (100% trimmings and 80% trimmings +20% minced fish) and three processing techniques (smoking, homemade and industrial cooking), and (2) in a 2 × 5 factorial scheme, using two processing techniques (homemade and industrial cooking) and five storage times under refrigeration (until 60 days). Mortadella elaborated with 100% trimmings, cooked by industrial immersion and in a homemade way, had physical-chemical and sensory characteristics that pleased the tasters more. This mortadella also remained within the microbiological and physical-chemical parameters recommended for consumption during 60 days of storage. It is recommended to cook mortadella by the industrial method, as it provides greater oxidative stability.
This study evaluated the genetic similarity and prevalence of the stx1, stx2, eae, and ehxA genes in Escherichia coli isolated from pasteurized cow milk. Eighty‐seven E. coli isolates from ...pasteurized cow milk from 22 dairies located in northwestern Paraná state, Brazil, were analyzed. Genetic similarity was evaluated using enterobacterial repetitive intergenic consensus sequence polymerase chain reaction (ERIC‐PCR) and repetitive extragenic palindromic sequence PCR (REP‐PCR). E. coli isolates were also analyzed by PCR to investigate the presence of the stx1, stx2, eae, and ehxA genes. ERIC‐PCR and REP‐PCR clustered 87 bacterial isolates in 76 and 81 genomic profiles, respectively. Both techniques revealed high genetic diversity among the E. coli isolates, confirming the possibility of their use in epidemiological studies. The stx1, stx2, eae, and ehxA virulence genes were not detected in E. coli isolates, indicating a low prevalence of Shiga toxin‐producing E. coli in milk produced in the region studied.
Current assay deals with the preparation of alfajores with different levels (0 to 15%) of dehydrated fish mixture of salmon (10%) and tilapia (90%) to assess the sensorial characteristics and their ...centesimal composition and microbiological. Fish inclusion in alfajores did not affect the aroma, taste, texture, color and physical aspect, with scores ranging between 6.70 and 7.96 of a hedonic scale of 9 score. An average score of 4 in a 5-score purchasing intention scale was obtained, or rather, tasters would probably buy the product. In the case of centesimal composition, inclusion affected (p <0.05) humidity (between 2.74 and 3.40%) and ash (between 0.31 and 1.01%) rates, with a quadratic effect; protein (between 5.35 and 7.12%) with positive linear effect; carbohydrates (between 72.64 and 75.79%) with negative linear effect. There was no difference (p >0.05) in lipids and calorie rates in the alfajores. Results show that the inclusion of up to 15% of a dehydrated mixture of salmon (10%) and tilapia (90%) in alfajores was greatly accepted and improved their nutrition values. Further, the product was also within the microbiological standards required by Brazilian sanitary laws.
Food contamination caused by enteric pathogens is a major cause of diarrheal disease worldwide, resulting in high morbidity and mortality and significant economic losses. Bacteria are important ...agents of foodborne diseases, particularly diarrheagenic Escherichia coli. The present study assessed the genetic diversity and antimicrobial resistance of E. coli isolates from pasteurized milk processed in 21 dairies in northwestern State of Parana, Brazil. The 95 E. coli isolates were subjected to antimicrobial susceptibility testing according to the recommendations of the Clinical and Laboratory Standards Institute and assessed genotypically by Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR). The highest rate of resistance was observed for cephalothin (55.78%). ERIC-PCR revealed high genetic diversity, clustering the 95 bacterial isolates into 90 different genotypic patterns. These results showed a heterogeneous population of E. coli in milk samples produced in the northwestern region of Paraná and the need for good manufacturing practices throughout the processing of pasteurized milk to reduce the risk of foodborne illnesses.
A contaminação de alimentos por patógenos entéricos é uma das principais causas de doenças diarréicas em todo o mundo, resultando em altas taxas de morbidade e mortalidade e perdas econômicas significativas. As bactérias são importantes agentes de doenças de origem alimentar, particularmente Escherichia coli diarreiogênicas. O presente estudo teve como objetivo avaliar a diversidade genética e a resistência a antimicrobianos de E. coli isoladas de leite pasteurizado, processados em 21 laticínios na região noroeste do Paraná - Brasil. Os 95 isolados de E. coli foram submetidos a testes de suscetibilidade aos antimicrobianos de acordo com as recomendações do Clinical and Laboratory Standards Institute e avaliados genotipicamente por ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus - Polymerase Chain Reaction). O principal perfil de resistência encontrado entre os isolados foi resistência à cefalotina (55,78%). ERIC-PCR revelou alta diversidade genética, agrupando os 95 isolados bacterianos em 90 diferentes perfis genotípicos. Estes resultados mostraram uma população heterogênea de E. coli em amostras de leite produzido na região noroeste do Paraná e a necessidade de boas práticas na manipulação de todo o processamento de leite pasteurizado, a fim de reduzir o risco de doenças transmitidas por alimentos.
O objetivo deste trabalho foi avaliar a qualidade microbiológica e a vida útil de filés de tilápia-do-nilo, submetidos a diferentes métodos de defumação e condições de armazenamento. Foram utilizados ...dois processos de defumação (a frio ou a quente), em filés com ou sem pigmentação. Os produtos foram armazenados sob refrigeração ou congelados, e monitorados por 28 dias para avaliação da vida útil. Os filés congelados foram monitorados continuamente por 146 dias, apenas para a análise de ácido tiobarbitúrico (TBA). Defumação a quente e a frio reduziram a quantidade de coliformes, respectivamente em 99,78% e 97,80%. O armazenamento do produto sob refrigeração permitiu a redução de 99,73% dos coliformes, e o armazenamento sob congelamento os reduziu em 99,83%. Os valores encontrados de coliformes fecais estiveram dentro do limite permitido. Os valores de TBA nos filés atingiram o máximo no 14o dia de armazenamento. Os valores de TBA nos tratamentos sob refrigeração foram superiores aos daqueles sob congelamento e, também, em filés defumados a frio, em comparação aos defumados a quente. O processo de defumação a quente, com posterior armazenamento sob congelamento, é a técnica mais apropriada para assegurar qualidade e maior período de vida útil para os filés de tilápia-do-nilo, independentemente do processo de pigmentação.
Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In the present study, the aim was to evaluate ...protein profile changes in S. Typhimurium biofilm treated with carvacrol. Proteomic analysis of treated versus untreated biofilm showed several changes in proteins involved with S. Typhimurium biofilm and antioxidant activity. The proteins DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and SODs (superoxide dismutases) A, B and C had their synthesis decreased after treatment with carvacrol. These proteins play a key role in S. Typhimurium biofilm formation, demonstrating the dynamic antibiofilm action of carvacrol. The differentially expressed proteins identified provide possible action targets for future studies in order to gain more insight into the mechanism of action of carvacrol on S. Typhimurium biofilm.
Kaftas with V-shaped filleting chips of the Nile tilapia (Oreochromis niloticus) were developed and the effects of the smoking technique on the characteristics of chemical composition, ...microbiological, sensory and benzo(a)pyrene were investigated. The filleting chips were ground and filleting included condiments and bacon. Kaftas were molded, frozen and distributed in a completely randomized design with three treatments (T 1 = baked in a grid; T 2 = smoked by friction and T 3 = smoked by liquid smoke) with 10 replications. The kaftas subjected to hot smoke had lower moisture content (13.97%), whereas the no-smoking kaftas had the highest content (20.49%). Kaftas with liquid smoke had high crude protein content (48.06%) and ash (9.49%), whereas the ash content was different only from no-smoking kaftas (8.79%). There was no significant difference in sensory parameters, except for flavor; smoked kaftas with liquid smoke were more accepted by the judges and the worst kaftas were no-smoked kaftas. Microbiological analysis showed that kaftas developed were appropriate to feed human beings within the required standards. Chips filleting is an alternative for the development of kaftas and those subjected to liquid smoke were considered the best.
Abstract This study evaluated the chemical composition, fatty acid profile, technological quality, sensory profile and microbiological characteristics of rolls with inclusion of 0, 5, 10 and 15% meal ...prepared with tilapia carcasses. Moisture and carbohydrate content were linearly reduced (P < 0.05) with increasing inclusion of tilapia meal, but a linear increase (P < 0.0001) was observed in protein (9.91 to 14.30%) and ash (1.74 to 4.65%) content. Lipids and calorific value of the rolls were not affected (P > 0.05) by inclusion levels, but 15% tilapia meal resulted in greater amount of polyunsaturated fatty acids (4.63%) in relation to 0% inclusion (4.46%). There was a linear increase (P < 0.0001) in the firmness of the breads (3.65 to 13.17N) and a linear decrease (P < 0.001) in volume and specific volume. Sensory attributes showed a negative linear effect (P < 0.01), with acceptance rates ranging from 85.24% (0% inclusion) to 70.57% (15% inclusion). It can be concluded that the inclusion of 5 to 15% tilapia meal in roll is effective to increase the protein, mineral matter, polyunsaturated fatty acids and the firmness of the roll, in addition to reducing carbohydrates and volume. Considering the reduction in sensory acceptance, it is recommended to use up to 10% tilapia meal in rolls.
This study aimed to evaluate the risk concerning child population's health because of the occurrence of AFM1 in UHT milk, powdered milk (PM) and infant formulae (IF). Determination of AFM1 was ...performed in 60 samples and evaluation of the mycotoxin exposure was carried out through the determination of the estimated daily intake (EDI), whereas risk characterization was evaluated with the calculation of the risk of Hepatocellular Carcinoma (HCC) and the Margin of Exposure (MOE). AFM1 ranged from 150 to 1020 ng/kg, and all the positive samples exceeded the limits stablished by European Community. The EDI for AFM1 ranged according to the age group of the population studied (0–5 years old) from 0.828 to 2.523, 0–2.113 and 0.029–0.833 ng/kg b. w./day in UHT, PM and IF, respectively. The number of HCC cases associated with AFM1 exposure (0.0015 a 0.0045) was higher than the limit of 0.001 case/100,000. MOE values for AFM1 were 728 to 239, considerably below the security margin of 10,000. These results point to a potential risk to the health of Brazilian child population exposed to AFM1 in dairy products.
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