Abstract With a rapid increase in industrial growth around the world, the demand for an entirely novel category of nanoparticles and technologies for wastewater treatment has become a key concern for ...environmental protection. Recently, hybrids of layered double hydroxides (LDH), particularly those containing LDH, have gained attention as potential nanoscale adsorbents for water treatment. Recent research has shown that LDH-containing composites are interesting versatile materials with the ability to be used in energy storage, photocatalysis, nanocomposites, and water treatment. In the current work, LDH-containing composites were utilized as adsorbents for the purpose of purifying water. The adsorbents investigated are Zn–Co–Fe/LDH/Chitosan-in situ sample preparation (LDH/CS1) and Zn–Co–Fe/LDH/Chitosan-ex situ sample preparation (LDH/CS2). Furthermore, LDH/CS1 and LDH/CS2 were investigated for wastewater treatment from methyl orange dye (MO) with various adsorption conditions. When the initial MO concentration was 20 mg/L and the amount of adsorbent was 0.1 g, the removal efficiency reached 72.8 and 91.7% for LDH/CS1 and LDH/CS2, respectively. The MO’s maximum adsorption capabilities are 160.78 and 165.89 mg/g for LDH/CS1 and LDH/CS2, respectively, which is much greater than that of comparable commercial adsorbents. MO adsorption onto LDH/CS1 and LDH/CS2 was best characterized by the pseudo-second-order kinetic model. The equilibrium adsorption data was followed by the Freundlich and Langmuir models. The adsorption is favorable as evidenced by the equilibrium parameter R L values for MO adsorption onto LDH/CS1 and LDH/CS2, which were 0.227 and 0.144, respectively. Using the free volume distribution method and the positron annihilation lifetime technique, the nanostructure of the materials was examined.
Guava is a nutritious fruit that has perishable behavior during storage. We aimed to determine the influences of some edible coatings (namely, cactus pear stem (10%), moringa (10%), and henna leaf ...(3%) extracts incorporated with gum Arabic (10%)), on the guava fruits’ properties when stored under ambient and refrigeration temperatures for 7, 14, and 21 days. The results revealed that the coating with gum Arabic (10%) only, or combined with the natural plant extracts, exhibited a significant reduction in weight loss, decay, and rot ratio. Meanwhile, there were notable increases in marketability. Moreover, among all tested treatments, the application of gum Arabic (10%) + moringa extract (10%) was the superior treatment for most studied parameters, and exhibited for the highest values for maintaining firmness, total soluble solids, total sugars, and total antioxidant activity. Overall, it was suggested that coating guava with 10% gum Arabic combined with other plant extracts could maintain the postharvest storage quality of the cold-storage guava.
The development of environmentally benign silicone composites from sugar palm fibre and silicone rubber was carried out in this study. The mechanical, physical, and morphological properties of the ...composites with sugar palm (SP) filler contents ranging from 0% to 16% by weight (wt%) were investigated. Based on the uniaxial tensile tests, it was found that the increment in filler content led to higher stiffness. Via dynamic mechanical analysis (DMA), the viscoelastic properties of the silicone biocomposite showed that the storage modulus and loss modulus increased with the increment in filler content. The physical properties also revealed that the density and moisture absorption rate increased as the filler content increased. Inversely, the swelling effect of the highest filler content (16 wt%) revealed that its swelling ratio possessed the lowest rate as compared to the lower filler addition and pure silicone rubber. The morphological analysis via scanning electron microscopy (SEM) showed that the sugar palm filler was evenly dispersed and no agglomeration could be seen. Thus, it can be concluded that the addition of sugar palm filler enhanced the stiffness property of silicone rubber. These new findings could contribute positively to the employment of natural fibres as reinforcements for greener biocomposite materials.
: HLA-DRB1 is the most polymorphic gene in the human leukocyte antigen (HLA) class II, and exon 2 is critical because it encodes antigen-binding sites. This study aimed to detect functional or marker ...genetic variants of HLA-DRB1 exon 2 in renal transplant recipients (acceptance and rejection) using Sanger sequencing.
: This hospital-based case-control study collected samples from two hospitals over seven months. The 60 participants were equally divided into three groups: rejection, acceptance, and control. The target regions were amplified and sequenced by PCR and Sanger sequencing. Several bioinformatics tools have been used to assess the impact of non-synonymous single-nucleotide variants (nsSNVs) on protein function and structure. The sequences data that support the findings of this study with accession numbers (OQ747803-OQ747862) are available in National Center for Biotechnology Information (GenBank database).
: Seven SNVs were identified, two of which were novel (chr6(GRCh38.p12): 32584356C>A (K41N) and 32584113C>A (R122R)). Three of the seven SNVs were non-synonymous and found in the rejection group (chr6(GRCh38.p12): 32584356C>A (K41N), 32584304A>G (Y59H), and 32584152T>A (R109S)). The nsSNVs had varying effects on protein function, structure, and physicochemical parameters and could play a role in renal transplant rejection. The chr6(GRCh38.p12):32584152T>A variant showed the greatest impact. This is because of its conserved nature, main domain location, and pathogenic effects on protein structure, function, and stability. Finally, no significant markers were identified in the acceptance samples.
Pathogenic variants can affect intramolecular/intermolecular interactions of amino acid residues, protein function/structure, and disease risk. HLA typing based on functional SNVs could be a comprehensive, accurate, and low-cost method for covering all HLA genes while shedding light on previously unknown causes in many graft rejection cases.
Federated clouds are interconnected cooperative cloud infrastructures offering vast hosting capabilities, smooth workload migration and enhanced reliability. However, recent devastating attacks on ...such clouds have shown that such features come with serious security challenges. The oblivious heterogeneous construction, management, and policies employed in federated clouds open the door for attackers to induce conflicts to facilitate pervasive coordinated attacks. In this paper, we present a novel proactive defense that aims to increase attacker uncertainty and complicate target tracking, a critical step for successful coordinated attacks. The presented systemic approach acts as a VM management platform with an intrinsic multidimensional hierarchical attack representation model (HARM) guiding a dynamic, self and situation-aware VM live-migration for moving-target defense (MtD). The proposed system managed to achieve the proposed goals in a resource-, energy-, and cost-efficient manner.
Phenaloenones are structurally unique aromatic polyketides that have been reported in both microbial and plant sources. They possess a hydroxy perinaphthenone three-fused-ring system and exhibit ...diverse bioactivities, such as cytotoxic, antimicrobial, antioxidant, and anti-HIV properties, and tyrosinase, α-glucosidase, lipase, AchE (acetylcholinesterase), indoleamine 2,3-dioxygenase 1, angiotensin-I-converting enzyme, and tyrosine phosphatase inhibition. Moreover, they have a rich nucleophilic nucleus that has inspired many chemists and biologists to synthesize more of these related derivatives. The current review provides an overview of the reported phenalenones with a fungal origin, including their structures, sources, biosynthesis, and bioactivities. Moreover, more than 135 metabolites have been listed, and 71 references have been cited. SuperPred, an artificial intelligence (AI) webserver, was used to predict the potential targets for selected phenalenones. Among these targets, we chose human glucose transporter 1 (hGLUT1) for an extensive in silico study, as it shows high probability and model accuracy. Among them, aspergillussanones C (60) and G (60) possessed the highest negative docking scores of −15.082 and −14.829 kcal/mol, respectively, compared to the native inhibitor of 5RE (score: −11.206 kcal/mol). The MD (molecular dynamics) simulation revealed their stability in complexes with GLUT1 at 100 ns. The virtual screening study results open up a new therapeutic approach by using some phenalenones as hGLUT1 inhibitors, which might be a potential target for cancer therapy.
Deficit irrigation water (DW) is one of the main stress factors that negatively affect cotton cultivation. Hence, the identification of cotton cultivars tolerant to DW and sandy soil conditions is ...particularly needed. Understanding the response of cultivars to DW is essential for estimating water needs. Besides, by understanding the physiological and antioxidant status, reflecting distinct growth, yield, and fiber quality traits under DW, the cultivar tolerant to DW can be identified in the early stage of plant growth. Therefore, two cotton cultivars (Giza 86 and Giza 92, selected for their suitability to the climatic conditions of the study area) were evaluated in this study under two DW regimes (80% or 60% of crop evapotranspiration; ETc) vs. complete irrigation water (CW; 100% of ETc as a control). These regimes amounted to 1228 or 922 vs. 1536 mm season−1, respectively, for field trials conducted during the 2019 and 2020 summer seasons. DW (80% or 60% of ETc) significantly decreased relative water content, membrane stability index, chlorophyll content, plant height, yield components, and fiber quality traits. Otherwise, phenolic compounds, proline contents, as well as antioxidant enzyme activities increased in concomitance with an increase in electrolyte leakage and malondialdehyde content. The harmful effects of the higher DW (60% of ETc) were more pronounced in both cultivars. However, compared to Giza 86, Giza 92 showed higher performance under both CW and DW regimes, accounting for higher values for all studied traits in the blooming stage. The correlation coefficient showed that most of the physiological traits and antioxidants under study were effective criteria in identifying a high-yielding cultivar under DW in the cotton blooming stage and therefore can be used to select the cotton cultivar more suitable for the conditions of the study area. Biplot analysis was used to study the relationship between DW and all evaluated traits, as it was found that the most prominent traits were elongation (%) with Giza 92 + 100% ETc, yellowness degree with Giza 86 + 100% ETc, and SOD with Giza 92 + 60% ETc.
The positron annihilation lifetime (PAL) technique was used to investigate the yield and lifetime of positronium (Ps) in three different samples of per‐fluorinated sulfonic ...acid/polytetrafluoroethylene (PTFE) proton exchange membranes. The high probability of Ps formation enables the study of the hole volume size by the PAL technique and explains the mechanism of both methanol permeation and proton conductivity of the membranes in relation to the relative humidity (RH). The influence of various membrane parameters, including water uptake, proton conductivity, and mechanical properties, was studied. The concentration of water molecules and SO3−–H3O+groups is correlated with the ion exchange capacity (IEC) of the membranes where higher IEC leads to high absorbance of water. The proton conductivity is enhanced by increasing the IEC value of the membrane. The activation energies of decomposition temperature for the F‐950 membrane are the lowest (Eb = 2.856 and 1.030 eV for the 1st and 2nd decomposition peaks, respectively, implying that the membrane with high IEC is thermally less stable. With increasing the IEC of the membranes, the concentration of the water molecules/SO3 group increases. In addition, the proton conductivity of the membrane is positively influenced by increasing water content at high RH. The fact that the variations in hole volume size and both methanol permeability and proton conductivity are highly dependent on RH suggests that hole volume size plays an important role in many parameters of hydrated perfluorinated sulfonic acid/PTFE proton exchange membranes. The outcomes data were introduced and interpreted appropriately with a theoretical model and compared with the literature.
The goal of the current study was to compare how well Nile tilapia,
Oreochromis niloticus
, utilized copper (Cu) as bulk and nano sources by evaluating fish growth, body indices, hematological ...assays, plasma metabolites, immune and anti-oxidative abilities, and intestinal morphometric measurements. The basal diet served as a control, with no Cu added, whereas the experimental diets were formed by adding bulk-Cu and nano-Cu to the basal diet to keep Cu levels at 3 and 6 mg kg
−1
, respectively, in both Cu sources. Tilapia (9.10 ± 0.014 g) were fed the control diet and four experimental diets for 12 weeks. Results indicated that growth, feed utilization, and body indices demonstrated a substantial improvement (
P
≤ 0.05) in tilapia that received a diet containing 3 and 6 Nano-Cu mg kg
−1
diet, compared to the performance of fish that received diets containing 3 and 6 Bulk-Cu mg kg
−1
diet and the control diet. Villi height, villi width, absorption area of villous (AAV), and mucosal to serosal amplification ratio (MSR) values demonstrated a substantial increase (
P
≤ 0.05) in tilapia fed 3 and 6 mg kg
−1
Nano-Cu compared to the values observed in fish fed the control and Bulk-Cu supplemented diets. Fish fed Bulk-Cu recorded the highest (
P
≤ 0.05) hemoglobin concentration in those fed 6 mg kg
−1
compared to 3 mg kg
−1
. Hematocrit value considerably improved (
P
≤ 0.05) by supplementation of Cu, whereas the highest significant value demonstrated in fish provided 6 mg/kg
−1
Nano-Cu. A fish-fed diet containing 3 mg kg
−1
Nano-Cu revealed the best (
P
≤ 0.05) values of plasma albumin, total protein, and globulins. Plasma HDL-C highest concentrations (
P
≤ 0.05) were reported in fish fed diet supplemented with 6 mg/kg
−1
either Bulk or Nano Cu, whereas values of plasma TG and VLDL-C declined as Cu supplementation level increased either from Bulk or Nano source. Also, the best (
P
≤ 0.05) values of CAT and GPX were seen in fish given diet supplemented with 6 Nano-Cu mg/kg
−1
. Fillets of fish-fed Nano-Cu-supplemented diets showed a marked decline (
P
≤ 0.05) in moisture and fat contents, while crude protein, ash, and Cu contents considerably increased in the fillet by dietary supplementation of Nano-Cu at both levels 3 and 6 mg kg
−1
. In conclusion, the supplemental diets with 3 or 6 Nano-Cu mg/kg
−1
enhanced growth, feed utilization, body indices, fillet nutrient composition, hematological assay, plasma metabolites, immune, antioxidant activities, and intestinal morphometry of Nile tilapia.