A new experimental model of whole uterus and ovary transplantation in the laboratory rat was achieved. The main goals of this study were concerned with developing and standardizing the microsurgical ...technique of uterus transplantation in rats and observing the particular cellular patterns of acute allograft rejection at the level of the transplanted graft. Thirty-five orthotopic uterus transplantations were performed. An additional 20 female rats were used for dissection training sessions. Recipients were euthanasied at 24 hours, 48 hours and 72 hours. Immediate postoperative survival was 100%. Patency of the microsurgical anastomoses, checked at 24 hours, was 100%. At 72 hours thrombosis occurred in all anastomoses. The explanted uterine grafts were fixed in formaline and analyzed under light microscopy and specific imunohistochemical analysis. The acute allograft rejection has a particular cellular reaction pattern, probably due to the unique diversity of the tissues that compose it. Inflammatory cells like LTCD8+, LBCD20+ and mastocytes tend to agglomerate in the vicinity of nervous and vascular structures, showing no signs of lymphoid tissue disposition like in typical acute rejection. Uterus transplantation in rats has proven to be a valid experiment that allows us to express hope that by further research on transplantation of the uterus gynecologists will be able to introduce an adapted technique in the treatment of specific cases of human female infertility.
In this study, ZnO has been used as inorganic shell material (in situ synthetized) for the encapsulation of KNO.sub.3, an inorganic molten salt commonly used in concentrated solar plants ...applications. The thermal stability of microparticles encapsulated by using a solvothermal process has been optimized by adjusting the parameters affecting the properties of the microparticles, such as the core:shell ratio and the temperature during the microencapsulation process. The energy stored and released after each thermal cycle was evaluated by differential scanning calorimetry. Chemical composition of microparticles was evaluated by infrared spectroscopy and inductively coupled plasma spectroscopy, as well as morphology was characterized by scanning electron microscopy. Results have shown the solvothermal synthesis as a feasible process for the microencapsulation of molten salts by obtaining KNO.sub.3 particles covered by ZnO microcrystals. These particles have thermal energy storage and release capacities and temperatures similar to those of raw KNO.sub.3, being the temperature used during the solvothermal process the parameter determining the thermal stability of the microparticles, as demonstrated by carrying out durability tests through consecutive heating-cooling thermal cycles (250-400 °C).
To investigate the length and three-dimensional orientation and to detail the morphological variations of the styloid process.
Forty-four patients undergoing temporal bone evaluation for different ...reasons were randomly selected and included in the present study. The length, angulation in the coronal and sagittal planes, as well as morphological variations of the styloid processes were assessed using conebeam computer tomography. Pearson's correlation coefficient was used to test possible associations between the length of styloid process and angulations, as well as between angulations. Student's t-test was used to compare the differences between the sample mean length and angulations in normal and elongated styloid process groups.
The sagittal angle showed weak positive correlations with the styloid process length and the transverse angle (r = 0.24, p = 0.02, n = 88). A medium positive correlation was found between the sagittal and transverse angulations in the elongated styloid process group (r = 0.49, p = 0.0015, n = 38). There was a statistical significant difference between the mean sagittal angulation in elongated styloid and normal styloid process groups (p = 0.015). The styloid process morphology also varied in terms of shape, number, and degree of ossification.
The morphometric and morphologic variations of the styloid process may be important factors to be taken into account not only from the viewpoint of styloid syndromes, but also in preoperatory planning and during surgery.
The present experiment aims to formulate and characterize a new phytotherapy ointment based on a total extract of Hypericum perforatum included in a novel ointment base. In order to investigate the ...healing properties of the ointment, in vivo experimental wound models of linear incision, circular excision and thermal burn were performed on Wistar rats. Topical treatment was achieved daily, for 21 days. Clinical and macroscopic evaluation, determination of wound contraction rate, period of re-epithelialization, and histopathological examination were achieved, along with the determination of the particle diameter and particle size distribution of the ointment. The results demonstrate that the tested novel ointment has significant wound healing effect in skin injuries and reveals to be safe for use.
This paper presents results of a systematic 2×2 MIMO channel measurement campaign, which has been carried out in order to analyze the impact of human body shadowing (HBS) on the 60 GHz wideband ...channel. A piecewise linear and two analytical models, namely the double knife-edge (DKE) model and a cylinder approach incorporating the uniform theory of diffraction (UTD), are used to approximate and to predict the measurement curves. We propose measures to quantify the degree of match between attenuation curves in the millimeter wave frequency band and use them to evaluate the model results with respect to different scenario groups. The presented results are beneficial to refine existing diffraction models regarding the prediction of HBS effects.
The study focuses on the macroscopic and microscopic aspects of the placentae resulting from abortions or febrile births and their correlation with acute disorders of the upper or lower respiratory ...apparatus in pregnant women in various stages of pregnancy. The viral, bacterial or mycotic disorders were considered responsible for triggering septic abortion, premature or full-term deliveries, followed by septic complications of the child/fetus or of the mother. When the mother's acute respiratory infection is induced by highly virulent pathogens, in patients with low immunity or lacking adequate medical treatment, the infection may spread through the mother's bloodstream to the placenta. The study was conducted on 90 placentae. Microscopic analysis of the tissue samples revealed acute inflammatory infiltration. Two of the study cases should be mentioned here: a four-month pregnant woman suffering from septic abortion and a nine-month pregnant woman whose fetus died in the womb because of acute pneumopathy on a non-breathing lung. Both pregnant women had the same type of disorder and neither followed any medical treatment prescribed by a physician. The prevention of placental infection is closely connected to the prevention of acute respiratory diseases or their proper treatment after their onset.
A presentation of the clinical and pathogenic aspects of choroidal ossification.
We report three clinical cases of choroidal ossification: choroidal osteoma, ossified choroidal hemangioma and total ...ossification of the choroid. The three patients underwent complete eye examination. The optical microscopy of a sample of ossified choroidal tissue revealed a spongy, osseous structure consisting of circular osseous lamellae, osteocytes, canaliculi and adipose tissue with microfoci of calcification.
Choroidal ossification is characterized by reduced frequency of occurrence, accessible clinical diagnosis, and unspecified pathogenesis. Several of the factors identified in the pathogenesis of intraocular ossification may play a role in the ossification of the choroid: chronic inflammatory cells, bone morphogenetic proteins, growth factors and mesenchymal stem cells. In addition to these factors, pericytes have a special role in the pathogenesis of choroidal ossification. Under the influence of bone morphogenetic proteins and growth factors, mesenchymal stem cells differentiate into osteoblasts. They secrete bone matrix (osteoid), whose regeneration and remodeling lead to the formation of bone tissue. The spongy bone structure of choroidal tissue points to a model of endoconjunctive/desmal ossification.
The knowledge of the clinical aspect of ossification of the choroids is required for the differential diagnosis with the posterior pole affections, and also for the prevention and treatment of secondary complications.
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