We have measured the beam-normal single-spin asymmetry in elastic scattering of transversely polarized 3 GeV electrons from unpolarized protons at Q2=0.15, 0.25 (GeV/c)2. The results are inconsistent ...with calculations solely using the elastic nucleon intermediate state and generally agree with calculations with significant inelastic hadronic intermediate state contributions. A(n) provides a direct probe of the imaginary component of the 2gamma exchange amplitude, the complete description of which is important in the interpretation of data from precision electron-scattering experiments.
Transverse single spin asymmetry, AN, of very forward π0 production from polarized p + p collisions provides new information toward an understanding of its production mechanism. AN of forward π0 in ...the pseudorapidity region of 3 < η < 4 has been described by the partonic structure of the proton in the perturbative QCD framework. However, recent data indicates a potential contribution from not only partonic but also diffractive interactions. In order to provide a new insight on the origin of the AN, we measured the very forward π0 production in the pseudorapidity region of 6 < η from √s = 510 GeV polarized p + p collisions at RHIC in 2017. We report our measurement of the very forward π0 over the transverse momentum range of 0 < pT < 1 GeV/c and the preliminary result.
Bioelectrical impedance analysis (BIA) has been used to estimate skeletal muscle mass, but its application in the elderly is not optimal. The accuracy of BIA may be influenced by the expansion of ...extracellular water (ECW) relative to muscle mass with aging. Multifrequency BIA (MFBIA) can evaluate the distribution between ECW and intracellular water (ICW), and thus may be superior to single-frequency BIA (SFBIA) to estimate muscle mass in the elderly. A total of 58 elderly participants aged 65-85 years were recruited. Muscle cross-sectional area (CSA) was obtained from computed tomography scans at the mid-thigh. Segmental SFBIA and MFBIA were measured for the upper legs. An index of the ratio of ECW and ICW was calculated using MFBIA. The correlation between muscle CSA and SFBIA was moderate (r = 0.68), but strong between muscle CSA and MFBIA (r = 0.85). ECW/ICW index was significantly and positively correlated with age (P < 0.001). SFBIA tends to significantly overestimate muscle CSA in subjects who had relative expansion of ECW in the thigh segment (P < 0.001). This trend was not observed for MFBIA (P = 0.42). Relative expansion of ECW was observed in older participants. The relative expansion of ECW affects the validity of traditional SFBIA, which is lowered when estimating muscle CSA in the elderly. By contrast, MFBIA was not affected by water distribution in thigh segments, thus rendering the validity of MFBIA for estimating thigh muscle CSA higher than SFBIA in the elderly.
Escherichia coli labile toxin (LT) was assessed as mucosal immunogen and as adjuvant for tetanus toxoid (TT) in mice. After oral administration of LT, C57BL/6(H-2b) and BALB/c (H-2d) mice were high ...mucosal and serum antibody responders, while C3H/HeN (H-2k) mice were low responders. High responders exhibited mainly serum IgG (including IgGl, IgG2a, and IgG2b), as well as IgM and IgA, while mucosal responses were IgA. Analysis of LT-B-specific CD4+ T helper (Th) cells from Peyer's patches (PP) or from spleen revealed a mixed Thl (interferon-γ) and Th2 (interleukin4 and -5) cell pattern. Oral LT given with TT induced TT-specific response patterns identical to LT-B. Analysis of mRNA from TT-specific PP CD4+ Th cells also revealed a mixed Thl- and Th2-type response. Thus, antibody response profiles induced by LT are regulated by both CD4+ Thl and Th2 cell types.
The Relativistic Heavy Ion Collider forward experiment is ready to take data in the RHIC Run2017 √s = 510 GeV p-p collisions using the LHCf Arm1 detector. New accelerator data are valuable to verify ...the Feynman scaling of FX1 production and to study the evolution of the break of scaling in neutron production. Using the transversely polarized proton beam, asymmetric production of forward neutrons is precisely measured. That is useful to understand the fundamental meson exchange in the proton-proton collisions.
Lys-gingipain (KGP), a lysine-specific cysteine proteinase, is one of the major virulence factors of Porphyromonas gingivalis. Here we examined the involvement of the catalytic domain of KGP ...(KGP(cd)) in hemoglobin binding by P. gingivalis, using a specific immunoglobulin G (IgG) elicited by the administration of plasmid DNA encoding KGP(cd) or the catalytic domain of Arg-gingipain (RGP(cd)). The pSeq2A/kgp(cd) and pSeq2B/rgp(cd) plasmids were constructed by the ligation of kgp(cd) and rgp(cd) DNA fragments, respectively. Female BALB/c mice were immunized with each of these plasmids. pSeq2A/kgp(cd) elicited a strong response to recombinant KGP(cd) (rKGP(cd)), as well as to comparably produced rRGP(cd)-reactive antibodies. The serum antibodies elicited by pSecTag2B/rgp(cd) also cross-reacted with rKGP(cd) as well as rRGP(cd). Anti-KGP(cd) IgG significantly inhibited hemoglobin binding by P. gingivalis. Furthermore, the inhibition of hemoglobin binding was markedly enhanced by a combination of anti-KGP(cd) and anti-fimbriae. Anti-RGP(cd) IgG showed a negligible inhibitory effect, while both anti-KGP(cd) and anti-RGP(cd) IgGs showed significant inhibitory effects on Lys- and Arg-specific proteolytic activities and on the growth of P. gingivalis under iron-restricted conditions where supplemented hemoglobin was the sole iron source. Immunized mice were challenged by intraperitoneal inoculation with P. gingivalis. All nonimmunized mice died within 72 h; however, vaccination with pSeq2A/kgp(cd) and pSeq2B/rgp(cd) prevented inflammatory responses and prolonged the survival rate of immunized mice by 43 and 27%, respectively. These results suggest that KGP(cd) acts as a hemoglobin-binding protein and can also be useful as an immunogen inducing a protective response to P. gingivalis infection.
Glioblastoma (GBM) is the most common malignant brain tumor and has “immunologically cold” features. Changing GBM to an “immunologically hot” tumor requires a strong trigger that induces initial ...immune responses in GBM. Allogeneic natural killer cells (NKCs) have gained considerable attention as promising immunotherapeutic tools against cancer, where gene-edited NKCs would result in effective anti-cancer treatment. The present study focused on the immune checkpoint molecule cytokine-inducible SH2-containing protein (CISH) as a critical negative regulator in NKCs.
We generated human primary CISH-deleted NKCs (NK dCISH) using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) with single guide RNA targeting genome sites on CISH coding exons. The genome-edited NKCs underwent microarray with differential expression analysis and gene set enrichment analysis (GSEA). The anti-GBM activity of the genome-edited NKCs was evaluated by apoptosis induction effects against allogeneic GBM cells and spheroids. We further detected in vivo antitumor effects using xenograft brain tumor mice.
We successfully induced human CISH-deleted NKCs (NK dCISH) by combining our specific human NKC expansion method available for clinical application and genome editing technology. CISH gene-specific guide RNA/Cas9 protein complex suppressed CISH expression in the expanded NKCs with high expansion efficacy. Comprehensive gene expression analysis demonstrated increased expression of 265 genes and decreased expression of 86 genes in the NK dCISH. Gene set enrichment analysis revealed that the enriched genes were involved in NKC effector functions. Functional analysis revealed that the NK dCISH had increased interferon (IFN) gamma and tumor necrosis factor (TNF) production. CISH deletion enhanced NKC-mediated apoptosis induction against allogeneic GBM cells and spheroids. Intracranial administration of the allogeneic NKCs prolonged the overall survival of xenograft brain tumor mice. Furthermore, the NK dCISH extended the overall survival of the mice.
The findings demonstrated the successful induction of human primary NK dCISH with CRISPR/Cas9 with efficient expansion. CISH deletion enhanced the NKC-mediated anti-tumor effects in allogeneic GBM and could be a promising immunotherapeutic alternative for patients with GBM.
The Brookhaven Relativistic Heavy Ion Collider (RHIC) has been providing collisions of polarized protons at a beam energy of 100 GeV since 2001. Equipped with two full Siberian snakes in each ring, ...polarization is preserved during acceleration from injection to 100 GeV. However, the intrinsic spin resonances beyond 100 GeV are about a factor of 2 stronger than those below 100 GeV making it important to examine the impact of these strong intrinsic spin resonances on polarization survival and the tolerance for vertical orbit distortions. Polarized protons were first accelerated to the record energy of 205 GeV in RHIC with a significant polarization measured at top energy in 2005. This Letter presents the results and discusses the sensitivity of the polarization survival to orbit distortions.
Experimental studies of the collisions of heavy nuclei at relativistic energies have established the properties of the quark–gluon plasma (QGP), a state of hot, dense nuclear matter in which quarks ...and gluons are not bound into hadrons1–4. In this state, matter behaves as a nearly inviscid fluid5 that efficiently translates initial spatial anisotropies into correlated momentum anisotropies among the particles produced, creating a common velocity field pattern known as collective flow. In recent years, comparable momentum anisotropies have been measured in small-system proton–proton (p+p) and proton–nucleus (p+A) collisions, despite expectations that the volume and lifetime of the medium produced would be too small to form a QGP. Here we report on the observation of elliptic and triangular flow patterns of charged particles produced in proton–gold (p+Au), deuteron–gold (d+Au) and helium–gold (3He+Au) collisions at a nucleon–nucleon centre-of-mass energy \\sqrt {s_{{\mathrm{NN}}}\ = 200 GeV. The unique combination of three distinct initial geometries and two flow patterns provides unprecedented model discrimination. Hydrodynamical models, which include the formation of a short-lived QGP droplet, provide the best simultaneous description of these measurements.
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