We investigate theoretically the band structure of flexural waves propagating in an elastic metamaterial thin plate. Kirchhoff-Love thin plate theory is considered. We study the influence of periodic ...arrays of multiple degrees of freedom local resonators in square and triangular lattices. Plane wave expansion and extended plane wave expansion methods, also known as ω(k) and k(ω), respectively, are used to solve the governing equation of motion for a thin plate. The locally resonant band gaps for square and triangular lattices present almost the same attenuation for all examples analysed. However, square lattice presents broader Bragg-type band gaps with higher attenuation than triangular lattice. An experimental analysis is conducted with a real elastic metamaterial thin plate with resonators in a square lattice. Modal analysis and forced response are computed by finite element method. Plane wave expansion, finite element and experimental results present good agreement.
•A new EEG bioelectrode lying between the wet and dry electrode concepts was developed.•The electrode expels 30μl of moistener upon the application of the adduction pressure.•Electrode design and ...polymer properties were optimized by finite element analysis.•In vivo EEG testing demonstrated a performance similar to commercial electrodes.
The present work reports on the development of a novel polymer-based electrode prototype for electroencephalography (EEG). The new electrode concept lies between the classic “wet” and “dry” electrodes, allowing addressing most of their drawbacks, while keeping the advantages of both. A localized skin hydration effect at the electrode/scalp contact point is achieved by the release of a small amount of a moisturizing agent (30μl) from a reservoir inside the electrode, triggered by the electrode/scalp adduction. The electrode's functionality relies on the polymer mechanical properties and on the design of an electrode reservoir that deforms upon the application of a specified adduction force. Numerical modeling tools were used for the definition and analysis of the electrode geometry and the material mechanical properties. The new electrode prototype proved to be able to monitor EEG signals similar to those of the commercial silver/silver chloride electrodes by using a fraction of the hydrating solution necessary for the commercial electrodes, thus avoiding to damage or dirtying the patient's hair. Furthermore, due to the small amount of moisturizing agent employed, the risk of conductive bridges between adjacent electrodes due to gel running is considerably reduced.
In this double-blind placebo-controlled randomized investigation, we assessed the tolerability of glutamine in older adults recruited from three daycare centers. The relevance of studying glutamine ...supplementation in elderly patients lies in its potential to provide a well-tolerated intervention. Glutamine, a crucial amino acid, plays a vital role in various physiological processes, including immune function and protein synthesis. Understanding its impact on older adults is essential, given the potential implications for their health and well-being. Participants received a daily dose of 12.4 g of oral effervescent glutamine (EGln group) or maltodextrin (placebo group) for 60 days. Fifteen patients from each group completed the study. The mean ages were 77.0±9.1 and 79.0±6.9 years for the EGln and placebo groups, respectively. We evaluated body mass index, aminogram, hemogram, plasma levels of glucose, prealbumin, albumin, urea, creatinine, uric acid, C-reactive protein, vitamin D, calcium, sodium, potassium, and the plasma activities of aspartate aminotransferase and alanine aminotransferase. Notably, we quantified a broad array of inflammatory markers and growth factors providing a holistic understanding of the potential effects of glutamine supplementation. The results demonstrated that oral glutamine did not induce significant changes in any evaluated parameters, and no adverse effects were reported. This finding suggested that the dosage of glutamine used in this study was well-tolerated and safe. This information contributes to the broader understanding of glutamine supplementation, emphasizing its safety and supporting its potential as a viable intervention for maintaining health in aging individuals.
The present study aimed to evaluate concentrations and forms of application of salicylic acid used for water stress mitigation on the gas exchange and growth of yellow passion fruit. The experimental ...design was arranged in randomized blocks in a 4 × 4 × 2 factorial scheme, with four concentrations of salicylic acid (SA) via foliar application (0.0, 0.7, 1.4, and 2.1 mM), four SA concentrations via fertigation (0.0, 0.7, 1.4, and 2.1 mM), and two irrigation depths estimated based on the actual evapotranspiration - ETr (50 and 100% of ETr), with three replications. Water stress negatively affected the physiology and growth of yellow passion fruit seedlings at 75 days after sowing (DAS). The application of salicylic acid, regardless of the form of application, attenuates the effects of water stress on gas exchange and growth of yellow passion fruit, with the best results obtained when applying a concentration of 1.30 mM via leaf or 0.90 mM via fertirrigation. The combination of foliar application of AS and fertigation contributed to improve photosynthetic and growth parameters under water conditions of 50 and 100% of ETr. The foliar application of AS presents superior responses to the application via fertigation. These results reinforce the hypothesis that the attenuation of water stress by salicylic acid is related to the maintenance of gas exchange, which depends on the concentration and form of application, and studies testing combinations throughout the crop cycle become promising for advances in knowledge from the action of this phytohormone on abiotic stress.
We quantify uncertainty in the impacts of climate change on the discharge of Rio Grande, a major tributary of the Paraná River in South America and one of the most important basins in Brazil for ...water supply and hydro-electric power generation. We consider uncertainty in climate projections associated with the greenhouse-gas emission scenarios (A1b, A2, B1, B2) and increases in global mean air temperature of 1 to 6° C for the HadCM3 GCM (Global Circulation Model) as well as uncertainties related to GCM structure. For the latter, multimodel runs using 6 GCMs (CCCMA CGCM31, CSIRO Mk30, IPSL CM4, MPI ECHAM5, NCAR CCSM30, UKMO HadGEM1) and HadCM3 as baseline, for a +2° C increase in global mean temperature. Pattern-scaled GCM-outputs are applied to a large-scale hydrological model (MGB-IPH) of Rio Grande Basin. Based on simulations using HadCM3, mean annual river discharge increases, relative to the baseline or control run period (1961–1990), by +5% to +10% under the SRES emissions scenarios and from +8% to +51% with prescribed increases in global mean air temperature of between 1 and 6° C. Substantial uncertainty in projected changes to mean river discharge (−28% to +13%) under the 2° C warming scenario is, however, associated with the choice of GCM. We conclude that, in the case of Rio Grande Basin, the most important source of uncertainty derives from the GCM rather than the emission scenario or the magnitude of rise in mean global temperature.
Oral tolerance blocks the development of specific immune responses to proteins ingested by the oral route. One of the first registries of oral tolerance showed that guinea pigs fed corn became ...refractory to hypersensitivity to corn proteins. Mice fed with chow containing corn are tolerant to zein, and parenteral injection of zein plus adjuvant blocks immunization to unrelated proteins injected concomitantly and reduces unspecific inflammation. Extensive and prolonged inflammatory infiltrate in the wound bed is one of the causes of pathological wound healing. Previous research shows that intraperitoneal injection of zein concomitant with skin injuries reduces the inflammatory infiltrate in the wound bed and improves wound healing. Herein, we tested if one subcutaneous injection of zein before skin injury improves wound healing. We also investigated how long the effects triggered by zein could improve skin wound healing. Mice fed zein received two excisional wounds on the interscapular skin under anesthesia. Zein plus Al(OH)3 was injected at the tail base at 10 min, or 3, 5, or 7 days before skin injuries. Wound healing was analyzed at days 7 and 40 after injury. Our results showed that a zein injection up to 5 days before skin injury reduced the inflammatory infiltrate, increased the number of T-cells in the wound bed, and improved the pattern of collagen deposition in the neodermis. These findings could promote the development of new strategies for the treatment and prevention of pathological healing using proteins normally found in the common diet.
Abstract Meat products represent an important component of the human diet and are a good source of nutrients. Food-borne microorganisms are the main pathogens that cause human diseases as a result of ...food consumption, especially products of animal origin. The objective of the present research was to verify the antibacterial activity of the essential oil of Thymus vulgaris against strains of Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus saprophyticus isolated from meat products. For this, the analyses of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were performed in microdilution plates. The association of the product with antimicrobials was also studied using disk diffusion. And the anti-adherent activity, which was determined in the presence of sucrose, in glass tubes. Thyme oil showed a strong inhibitory activity against K. pneumoniae, P. aeruginosa and S. saprophyticus, with the MIC values ranging from 64 to 512 μg/mL, and bactericidal effect for most strains, with MBC values ranging from 256 to 1,024 μg/mL. T. vulgaris oil exhibited varied interactions in association with the antimicrobials, with synergistic (41.67%), indifferent (50%) and antagonistic (8.33%) effects. Regarding the anti-adherent activity, the test product was effective in inhibiting the adherence of all bacterial strains under study. Therefore, thyme oil presents itself as an antibacterial and anti-adherent agent against K. pneumoniae, P. aeruginosa and S. saprophyticus, being a natural product that can represent an interesting alternative in the efforts to combat foodborne diseases.
Resumo Os produtos cárneos representam um importante componente da dieta humana e constituem uma boa fonte de nutrientes. Microrganismos de origem alimentar são os principais patógenos que causam doenças humanas como resultado do consumo de alimentos, principalmente, produtos de origem animal. O objetivo da presente pesquisa foi verificar a atividade antibacteriana do óleo essencial de Thymus vulgaris frente às cepas de Klebsiella pneumoniae, Pseudomonas aeruginosa e Staphylococcus saprophyticus isoladas de produtos cárneos. Para isso, foram realizadas as análises de Concentração Inibitória Mínima (CIM) e a Concentração Bactericida Mínima (CBM) em placas de microdiluição. Assim como, o estudo de associação do produto com antimicrobianos, realizado por difusão em disco. E a atividade antiaderente, que foi determinada na presença de sacarose, em tubos de vidro. O óleo de tomilho apresentou uma forte atividade inibitória contra K. pneumoniae, P. aeruginosa e S. saprophyticus, com os valores de CIM variando entre 64 a 512 μg/mL, e efeito bactericida para a maioria das cepas, com valores de CBM entre 256 a 1.024 μg/mL. O óleo de T. vulgaris exibiu interações variadas na associação com os antimicrobianos, com efeitos sinérgicos (41,67%), indiferente (50%) e antagonista (8,33%). Em relação a atividade antiaderente, o produto teste foi eficaz na inibição a aderência de todas cepas bacterianas em estudo. Portanto, o óleo de tomilho apresenta-se como agente antibacteriano e antiaderente frente a K. pneumoniae, a P. aeruginosa e a S. saprophyticus, sendo um produto natural que pode representar uma alternativa interessante nos esforços para combater doenças transmitidas por alimentos.
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