Schwann cells play a critical role in the development of the peripheral nervous system (PNS), establishing important relationships both with the extracellular milieu and other cell types, ...particularly neurons. In this review, we discuss various Schwann cell interactions integral to the proper establishment, spatial arrangement, and function of the PNS. We include signals that cascade onto Schwann cells from axons and from the extracellular matrix, bidirectional signals that help to establish the axo‐glial relationship and how Schwann cells in turn support the axon. Further, we speculate on how Schwann cell interactions with other components of the developing PNS ultimately promote the complete construction of the peripheral nerve.
Nakamura, FY, Antunes, P, Nunes, C, Costa, JA, Esco, MR, and Travassos, B. Heart rate variability changes from traditional vs. ultra-short-term recordings in relation to preseason training load and ...performance in futsal players. J Strength Cond Res 34(10): 2974-2981, 2020-The aims of this study were to analyze heart rate variability (HRV) changes in futsal players during preseason training using both "criterion" (10-minute) and ultra-shortened (2-minute) measures, and to determine whether they were related to changes in the Yo-Yo Intermittent Recovery Test, level 1 (Yo-Yo IR1) performance and accumulated training load (TL). Eleven male competitive futsal players (age = 25.19 ± 4.70 years; body mass = 73.15 ± 11.70 kg; and height = 176.90 ± 5.01 cm) volunteered for the study. Data collection took place during the first to the fourth weeks of preseason training. Training load was monitored with session ratings of perceived exertion. The total distance (TD) covered during the Yo-Yo IR1 was recorded during week 1 and week 4. Heart rate variability was measured through the log-transformed root mean square of successive normal-to-normal interval differences using the ultra-short analysis, with its weekly mean (lnRMSSDM) and coefficient of variation (lnRMSSDCV) recorded, and by means of the criterion method (weeks 1 and 4). lnRMSSDM was likely higher at week 4 compared with week 1 using both criterion and ultra-short recordings. Moderate-to-large correlations were found between changes in the lnRMSSDM and lnRMSSDCV values and changes in TL and TD (r values ranged from -0.48 to 0.65). Changes in ultra-short HRV measures (i.e., increase in lnRMSSDM and decrease in lnRMSSDCV) during futsal preseason were associated with increased performance. The players who accumulated higher perceived TLs displayed smaller improvements in the Yo-Yo IR1 performance and HRV.
Loturco, I, Nunes, RFH, Lampert, RR, Silva, RLP, Hespanhol, JE, Novack, LF, Conde, JHS, Pereira, LA, and McGuigan, MR. Effects of two different low-volume resistance training programs applied during ...the off-season period on the speed-power performance of elite youth soccer players. . J Strength Cond Res 38(3): 571-576, 2024-The aim of this study was to analyze the changes in the speed-power performance of elite youth soccer players submitted to 2 different low-volume resistance training programs during the off-season period. Twenty under-17 players were randomly allocated to "traditional nonballistic" or "ballistic training" groups. Countermovement jump (CMJ), 20-m sprinting speed, and half-squat (HS) power tests were performed after the final match of the season (pretesting session) and at the beginning of the subsequent season (post-testing session), after 4 weeks of detraining. Between-group differences were assessed using a 2-way ANOVA with repeated measures followed by the Tukey's post hoc test. Performance variations were individually analyzed with the use of the "true changes" calculation. At post-tests, CMJ height and HS power remained unchanged ( p > 0.05) but similar and significant improvements in sprint speed were observed in both groups ( p < 0.05). However, notably, a larger number of players in the ballistic group exhibited "true changes" in HS power (i.e., 55 vs. 33%, compared with the traditional group, respectively). In conclusion, either low-volume ballistic or traditional resistance training schemes were able to increase sprint speed and maintain power output during a short interseason break in youth soccer players. Despite this apparent similarity, at the individual level, ballistic movements were more efficient at improving lower-body power. Practitioners can use the strategies described here to improve the sprint and power performance of soccer players during short periods of soccer-specific training cessation.
Calorie restriction extends lifespan and produces a metabolic profile desirable for treating diseases of ageing such as type 2 diabetes. SIRT1, an NAD+-dependent deacetylase, is a principal modulator ...of pathways downstream of calorie restriction that produce beneficial effects on glucose homeostasis and insulin sensitivity. Resveratrol, a polyphenolic SIRT1 activator, mimics the anti-ageing effects of calorie restriction in lower organisms and in mice fed a high-fat diet ameliorates insulin resistance, increases mitochondrial content, and prolongs survival. Here we describe the identification and characterization of small molecule activators of SIRT1 that are structurally unrelated to, and 1,000-fold more potent than, resveratrol. These compounds bind to the SIRT1 enzyme-peptide substrate complex at an allosteric site amino-terminal to the catalytic domain and lower the Michaelis constant for acetylated substrates. In diet-induced obese and genetically obese mice, these compounds improve insulin sensitivity, lower plasma glucose, and increase mitochondrial capacity. In Zucker fa/fa rats, hyperinsulinaemic-euglycaemic clamp studies demonstrate that SIRT1 activators improve whole-body glucose homeostasis and insulin sensitivity in adipose tissue, skeletal muscle and liver. Thus, SIRT1 activation is a promising new therapeutic approach for treating diseases of ageing such as type 2 diabetes.
Nanodelivery systems for cutaneous melanoma treatment Pereira, Irina; Monteiro, Carina; Pereira-Silva, Miguel ...
European journal of pharmaceutics and biopharmaceutics,
March 2023, 2023-Mar, 2023-03-00, 20230301, Letnik:
184
Journal Article
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•Cutaneous melanoma (CM) is a multifactorial and heterogeneous disease.•Understanding CM molecular biology is crucial to design targeted nanomedicines.•Biomimetic nanovaccines may ...constitute future strategies for CM immunotherapy.•Magnetic nanosystems can be used as CM theranostic platforms.•Different advanced CM therapeutic modalities can be combined using nanosystems.
Cutaneous melanoma (CM) is a multifactorial disease whose treatment still presents challenges: the rapid progression to advanced CM, which leads to frequent recurrences even after surgical excision and, notably, the low response rates and resistance to the available therapies, particularly in the case of unresectable metastatic CM. Thereby, alternative innovative therapeutic approaches for CM continue to be searched.
In this review we discuss relevant preclinical research studies, and provide a broad-brush analysis of patents and clinical trials which involve the application of nanotechnology-based delivery systems in CM therapy.
Nanodelivery systems have been developed for the delivery of anticancer biomolecules to CM, which can be administered by different routes. Overall, nanosystems could promote technological advances in several therapeutic modalities and can be used in combinatorial therapies.
Nevertheless, the results of these preclinical studies have not been translated to clinical applications. Thus, concerted and collaborative research studies involving basic, applied, translational, and clinical scientists need to be performed to allow the development of effective and safe nanomedicines to treat CM.
•Adsorption of cellulases on lignin depends on temperature.•Adsorption at 45 °C exceeds that at 30 °C for liquid hot water pretreated bagasse.•Acid treated bagasse adsorbs enzymes more ...strongly.•Results explain lower sensitivity of SSF (at 30 °C) to inhibitors.
Extents of adsorption of cellulolytic enzymes on lignin, derived from sugarcane bagasse, were an inverse function of incubation temperature and varied with type of lignin extraction. At 45 °C, lignin derived from acid hydrolyzed liquid hot water pretreated bagasse completely adsorbed cellulolytic enzymes from Trichoderma reesei within 90 min. Lignin derived from enzyme hydrolyzed liquid hot water pretreated bagasse adsorbed only 60% of T. reesei endoglucanase, exoglucanase and β-glucosidase activities. β-Glucosidase from Aspergillus niger was not adsorbed. At 30 °C, adsorption of all of the enzymes was minimal and enzyme hydrolysis at 30 °C approached that at 45 °C after 168 h. Hence, temperature provided an approach to decrease loss of enzyme activity by reducing enzyme adsorption on lignin. This helps to explain why simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP), both carried out at 30–32 °C, could offer viable options for mitigating lignin-derived inhibition effects.
The effect of the support size on the properties of enzyme immobilization was investigated by using chitosan macroparticles and nanoparticles. They were prepared by precipitation and ionotropic ...gelation, respectively, and were characterized by Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), transmission electron microscopy (TEM), light scattering analysis (LSA), and N2 adsorption–desorption isotherms. β-Galactosidase was used as a model enzyme. It was found that the different sizes and porosities of the particles modify the enzymatic load, activity, and thermal stability of the immobilized biocatalysts. The highest activity was shown by the enzyme immobilized on nanoparticles when 204.2 mg protein·(g dry support)−1 were attached. On the other hand, the same biocatalysts presented lower thermal stability than macroparticles. β-Galactosidase immobilized on chitosan macro and nanoparticles exhibited excellent operational stability at 37 °C, because it was still able to hydrolyze 83.2 and 75.93% of lactose, respectively, after 50 cycles of reuse.
Neuronal excitation imposes a high demand of ATP in neurons. Most of the ATP derives primarily from pyruvate-mediated oxidative phosphorylation, a process that relies on import of pyruvate into ...mitochondria occuring exclusively via the mitochondrial pyruvate carrier (MPC). To investigate whether deficient oxidative phosphorylation impacts neuron excitability, we generated a mouse strain carrying a conditional deletion of MPC1, an essential subunit of the MPC, specifically in adult glutamatergic neurons. We found that, despite decreased levels of oxidative phosphorylation and decreased mitochondrial membrane potential in these excitatory neurons, mice were normal at rest. Surprisingly, in response to mild inhibition of GABA mediated synaptic activity, they rapidly developed severe seizures and died, whereas under similar conditions the behavior of control mice remained unchanged. We report that neurons with a deficient MPC were intrinsically hyperexcitable as a consequence of impaired calcium homeostasis, which reduced M-type potassium channel activity. Provision of ketone bodies restored energy status, calcium homeostasis and M-channel activity and attenuated seizures in animals fed a ketogenic diet. Our results provide an explanation for the seizures that frequently accompany a large number of neuropathologies, including cerebral ischemia and diverse mitochondriopathies, in which neurons experience an energy deficit.
A thorough characterization of the genetic diversity of viruses present in vector and vertebrate host populations is essential for the early detection of and response to emerging pathogenic viruses, ...yet genetic characterization of many important viral groups remains incomplete. The Simbu serogroup of the genus Orthobunyavirus, family Bunyaviridae, is an example. The Simbu serogroup currently consists of a highly diverse group of related arboviruses that infect both humans and economically important livestock species. Here, we report complete genome sequences for 11 viruses within this group, with a focus on the large and poorly characterized Manzanilla and Oropouche species complexes. Phylogenetic and pairwise divergence analyses indicated the presence of high levels of genetic diversity within these two species complexes, on a par with that seen among the five other species complexes in the Simbu serogroup. Based on previously reported divergence thresholds between species, the data suggested that these two complexes should actually be divided into at least five species. Together these five species formed a distinct phylogenetic clade apart from the rest of the Simbu serogroup. Pairwise sequence divergences among viruses of this clade and viruses in other Simbu serogroup species complexes were similar to levels of divergence among the other orthobunyavirus serogroups. The genetic data also suggested relatively high levels of natural reassortment, with three potential reassortment events present, including two well-supported events involving viruses known to infect humans.
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