The lungworm
is one of the main causes of respiratory diseases in cats worldwide. This report describes the unusual case of a kitten infected with
and presented to a veterinary clinic in Brazil with ...lethargy, dysphagia, non-ambulatory tetraplegia, and pelvic limbs bilateral myoclonus. The clinical picture of the kitten worsened with generalized flaccid tetraplegia and death a few days after hospitalization. At necropsy, hemorrhagic necrosis and subarachnoid hemorrhages were detected in several areas of the central nervous system. Nematode stages were found at post-mortem histological examinations in lungs, cerebellum, subarachnoid space of the brain and spinal cord. Microscopic and molecular (PCRs-coupled-sequencing protocols) examination showed the presence of
in histological samples. This study describes the first neurological aelurostrongylosis due to ectopic localization of adult worms in the central nervous system of a cat, causing acute hemorrhagic multifocal meningoencephalomyelitis. Further studies are necessary to elucidate whether unusual localizations and the migration of
are more frequent than expected.
To evaluate the efficacy and safety of transarterial chemoembolization with polyethylene glycol (PEG) drug-eluting embolic agents in the treatment of hepatocellular carcinoma (HCC).
A single-center ...retrospective study of 302 patients (258 men; 85.4%) with HCC treated during a 20-month period was conducted. The mean patient age was 66 years ± 10; 142 (47%) had Barcelona Clinic Liver Cancer stage A disease and 134 had (44.4%) stage B disease; 174 (57.6%) had a single HCC tumor, 65 (21.5%) had 2, and 62 (20.9%) had 3 or more. Mean index tumor size was 36.6 mm ± 24.8. One-month follow-up computed tomography (CT) response per modified Response Evaluation Criteria In Solid Tumors and clinical and biochemical safety were analyzed. Progression-free and overall survival were calculated by Kaplan–Meier method.
Median follow-up time was 11.9 months (95% confidence interval, 11.0–13.0 mo). One-month follow-up CT revealed complete response in 179 patients (63.2%), partial response in 63 (22.3%), stable disease in 16 (5.7%), and progressive disease in 25 (8.8%). The most frequent complications were postembolization syndrome in 18 patients (6%), liver abscess in 5 (1.7%), and puncture-site hematoma in 3 (1%). Biochemical toxicities occurred in 57 patients (11.6%). Survival analysis at 12 months showed a progression-free survival rate of 65.9% and overall survival rate of 93.5%. Patients who received transplants showed a 57.7% rate of complete pathologic response.
Chemoembolization with PEG embolic agents for HCC is safe and effective, achieving an objective response rate of 85.5%.
Objective Our objective was to evaluate whether there is an enrichment of rare variants in familial hemophagocytic lymphohistiocytosis (HLH)‐associated genes among patients with systemic juvenile ...idiopathic arthritis (sJIA) with or without macrophage activation syndrome (MAS). Methods Targeted sequencing of HLH genes ( LYST , PRF1 , RAB27A , STX11 , STXBP2 , UNC13D ) was performed in patients with sJIA from an established cohort. Sequence data from control participants were obtained in silico (database of Genotypes and Phenotypes: phs000280.v8.p2). Rare variant association testing (RVT) was performed with sequence kernel association test package. Significance was defined as P < 0.05 after 100,000 permutations. Results Sequencing data from 524 sJIA cases were jointly called and harmonized with exome‐derived target data from 3,000 controls. Quality control operations produced a set of 480 cases and 2,924 ancestrally matched control participants. RVT of cases and controls revealed a significant association with rare protein‐altering variants (minor allele frequency MAF < 0.01) of STXBP2 ( P = 0.020) and ultrarare variants (MAF < 0.001) of STXBP2 ( P = 0.006) and UNC13D ( P = 0.046). A subanalysis of 32 cases with known MAS and 90 without revealed a significant difference in the distribution of rare UNC13D variants ( P = 0.0047) between the groups. Additionally, patients with sJIA more often carried two or more HLH variants than did controls ( P = 0.007), driven largely by digenic combinations involving LYST . Conclusion We identified an enrichment of rare HLH variants in patients with sJIA compared with controls, driven by STXBP2 and UNC13D. Biallelic variation in HLH genes was associated with sJIA, driven by LYST . Only UNC13D displayed enrichment in patients with MAS. This suggests that HLH variants may contribute to the pathophysiology of sJIA, even without MAS. image
Melanomas are tumors arising from externally uncontrolled melanocytes that produce varying amounts of melanin. In this study, we report a case of melanoma with neurological impairment without ...evidence of cutaneous neoplastic lesions in an adult buffalo in the state of Pará, Brazil. Clinically, the buffalo exhibited apathy, decreased mandibular tone, and occasionally an open mouth with motor incoordination, and eventually succumbed to the condition. Necropsy revealed multifocal tumor masses in the brain, pituitary gland, trigeminal ganglion, and spinal cord. The neoplastic cells showed strong positive signals for vimentin, Melan-A, PNL-2, and SOX10. The diagnosis was made via necropsy, histopathology, and positive immunostaining for Melan-A and PNL-2, which are specific markers for melanocyte identification.
Mucopolissacaridose em um cão: relato de caso Veiga, C. C. P.; Oliveira, L. I.; Silva, R. S. ...
Revista brasileira de medicina veterinária,
2017, Letnik:
39, Številka:
4
Journal Article
Recenzirano
Odprti dostop
A Mucopolissacaridose é uma doença rara, multissistêmica, progressiva e sem tratamento. Os objetivos deste estudo foram descrever os achados clínicos, radiográficos e anátomo-patológicos de um caso ...de mucopolissacaridose em um cão. Relata-se um caso de um cão, macho, Fox Paulistinha, de quatro meses de idade, oriundo de cruzamento com alto grau de endogamia. O cão apresentava apatia, dificuldades de locomoção, aumento de volume nas articulações, opacidade de córnea e dispneia inspiratória. Ao exame radiográfico observaram-se alterações bilaterais em linhas fiseais de vértebras, rádio e ulna, hepatomegalia e hipoplasia traqueal. Devido à acentuada dificuldade respiratória, o proprietário optou pela eutanásia. À necropsia, observou-se ausência das cartilagens da laringe, cricoide e aritenoide, coração globoso, e fígado e baço moderadamente aumentados. As alterações ósseas incluíram aumento do perímetro do crânio, má formação do esterno e costelas sinuosas. A histopatologia revelou displasia nas cartilagens da epiglote, da traqueia e articulares, nas quais os condrócitos apresentavam-se com citoplasma vacuolizado; havia ainda acentuado espessamento da íntima da aorta. O histórico de cruzamentos com alto grau de endogamia, associado aos achados clínicos, radiográficos e anátomo-patológicos confluem para o diagnóstico de mucopolissacaridose.
Angiolipoma in a Dog Correia Oliveira, Mariana; Jorge Chipitelli de Carvalho, Marcelo; Manhãs de Souza Basto, Jade ...
Acta Scientiae Veterinariae,
01/2021, Letnik:
49
Journal Article
Recenzirano
Odprti dostop
Background: Angiolipoma is a benign tumor composed of endothelial cells and mature adipocytes. Tumors reported in domestic species include two variants; infiltrative or non-infiltrative. Bitches and ...intact males seem predisposed. This mesenchyme tumor is commonly mistaken with lipoma due to its soft texture and subcutaneous site and often requires histopathology to confirm its diagnosis. Microscopic examination also enables the evaluation of surgical margins and rule out possible infiltrative sites. Complete surgical excision is usually curative. This study reports a case of non-infiltrating angiolipoma in a dog.Case: A 14-year-old mixed-breed dog was presented to a veterinary clinic in the city of Rio de Janeiro, RJ, Brazil. On palpation, a painless mass was noted, with high mobility and covered by intact hirsute skin in the right subcutaneous ventrolateral region. Computed tomography of the chest showed an expansive mass of uptake only from the edges of the soft tissues of the right subcutaneous ventrolateral region. The mass was homogeneous and well delimited, suggesting a neoplastic process. Subsequently, the mass was surgically removed, fixed in 10% buffered formalin, and sent for histopathological analysis. On macroscopic examination, the mass was well delimited, without skin coverage, and measured 2.3 × 1.9 × 0.6 cm. The consistency was smooth and unctuous in appearance with a compact cream-colored surface with blackish multifocal spots. Under microscopy, the histological sections showed neoplasm of mature adipocytes and of endothelial cells of blood vessels benign were filled with a marked amount of red blood cells. Multifocal fibrin thrombi and a mild inflammatory infiltrate composed of lymphocytes and rare mast cells were evident. There was no infiltration in the regional skeletal musculature. Thus, a diagnosis of non-infiltrative angiolipoma was established.Discussion: The diagnosis of non-infiltrating angiolipoma in this case was established through the results of histopathological examination. The occurrence of this neoplasm in dogs is uncommon, and the data reported in the veterinary medicine literature are scarce. However, in this study, it was found that the neoplasm on screening presented a behavior like that of lipomas, with noninvasive growth and the absence of local recurrence. The canine species does not commonly convey pain on palpation during a clinical examination, as observed in the present case. In humans, multiple angiolipoma nodules are common; this clinical presentation differs from that in animals, in which solitary nodules are generally observed. In dogs, as in the present case, they seem to have a predilection for the trunk. In animals, the pathogenesis of angiolipomas is not established, but in humans, it is based on theories that include the reaction to harmful stimuli and congenital malformation of adipose tissue. In humans, the presence of fibrin thrombi on the periphery of the region of cell proliferation are microscopic findings that can assist in the diagnosis of angiolipomas, an approach that was implemented in the present case. The occurrence of this neoplasm in dogs is uncommon, and the data reported in the veterinary medicine literature are scarce. The importance of an adequate description of angiolipomas is based on the need to provide information about its epidemiology, biological behavior, and prognosis.
•A low cost TRM composite is proposed to strengthen adobe vaults.•The strengthening techniques are tested by imposing increasing cyclic displacements.•The tested techniques improve the vaults’ ...structural behavior.•The low cost TRM composite applied at the extrados improves the ultimate displacement.
This paper deals with the structural behaviour and strengthening of adobe vaults representative from historical buildings of the city of Yazd, Iran. Eight destructive tests were performed on 1:3 scaled adobe vault models by loading them at 30% of the span in loading–unloading cycles of increasing vertical displacement. The models were tested considering both unstrengthened and strengthened conditions, where the strengthening consisted of an externally bonded low-cost textile reinforced mortar (LC-TRM) composed of a low-cost fiber-glass mesh embedded in earth-based mortar. Three different strengthening strategies were evaluated, namely by testing undamaged models strengthened at the extrados, undamaged models strengthened at the intrados and repaired models strengthened at the intrados. The experimental results show that the adopted LC-TRM composite improves the structural behavior of adobe vaults, by increasing substantially their load-bearing capacity and by changing the failure mechanism to more ductile ones.
Diversos modelos cirúrgicos são propostos para a retirada das neoplasias mamárias em cadelas, no entanto, os estudos de comparação entre as técnicas são escassos. O objetivo principal deste ...trabalho foi estabelecer um modelo de procedimento clínico e cirúrgico no tratamento das neoplasias mamárias em cadelas, similar ao observado em mulheres e designado como quadrantectomia. Foram atendidas 154 cadelas no Serviço de Obstetrícia do Hospital Veterinário da UFRRJ no período compreendido entre março de 2014 a dezembro de 2015. Destas, 141 cadelas com diversos tipos de neoplasias mamárias foram operadas. Na avaliação pré-operatória foram realizados exames complementares para determinação do estadiamento tumoral (Sistema TNM) e do risco cirúrgico. A pré-anestesia foi realizada com Acepromazina 0,2% (0,2 mg/kg, IM) ou Diazepan 0,5% (0,5 mg/kg, IM). A indução anestésica geral foi feita com Diisopropilfenol 1% (2,0 a 8,0 mg/kg, IV) e a manutenção anestésica geral com Isoflurano ofertado por traqueotubo e diluído em oxigênio a 100%, em circuito fechado de anestesia. O emprego da técnica de quadrantectomia para exérese de neoplasias mamárias constituiu uma manobra preventiva ao surgimento de novas massas tumorais, comprovada mediante a taxa de sobrevivência de 95,5%, em comparação com o emprego da técnica de mastectomia unilateral direita ou esquerda ou ainda a mastectomia bilateral total, cuja taxa de sobrevivência foi de 90,5%. A técnica é facilmente executável, associada ao método de dissecção, uso de dreno cirúrgico e curativo compressivo.
•Fulvic acids increase paclobutrazol absorption by mango plant.•Fulvic acids associated to paclobutrazol inhibit gibberellin biosynthesis quickly.•Fulvic acids associated to paclobutrazol promote ...uniform flowering.•Fulvic acids associated to paclobutrazol increase mango fruit yield.
The present study aimed to assess the effectiveness of fulvic acids and free amino acids in potentiating the effect of paclobutrazol on flowering management of mango 'Keitt' grown in tropical semiarid. The experiment was carried out simultaneously in two orchards with the same mango cultivar and management characteristics. The experiment was performed from 2017 to 2018 in two experimental orchards located in Cabrobó, State of Pernambuco, Brazil. The experimental design was randomized blocks with four treatments, five replications and four trees per replication. The treatments consisted of several combinations of paclobutrazol with fulvic acids and free amino acids, as follows: T1: paclobutrazol + water (control); T2: paclobutrazol + fulvic acids; T3: paclobutrazol + free amino acids; and T4: paclobutrazol + fulvic acids + free amino acids. According to the results, the use of fulvic acids, free amino acids, or both affect the PBZ action in ‘Keitt’mango trees. The addition of fulvic acids to PBZ improves its efficiency by inhibiting gibberellin content at the most appropriate time, maintaining carbohydrate, protein and amino acids levels, uniform flowering and, consequently, providing higher yield in mango ‘Keitt,’ and therefore may be recommended for growing mangoes in tropical semiarid conditions.
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